FastQCFastQC Report
Mon 16 Jan 2017
ERR1146672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103222
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63300.041.83234894
GTACTAG71550.034.1723821
CAACGCA107700.032.7706535
GCATCGT70250.031.5736318
TAGGCAT82950.031.3873235
TACCCCG4850.031.0076685
CTGTGCG93350.030.606319
GTATCAA131600.030.4771751
ATCAACG116300.030.3486183
TAGTACT98850.029.5725544
TCTAACG36900.028.4025382
TATTCTC34300.028.3620175
ACTGTGC105050.028.31638
CTAACGC37200.028.1734873
TAACGCC37650.027.8365234
GTACTGT105800.027.671636
CTAGGCA97400.027.5519454
CGCCTAC38100.027.3814747
GGTATCA87200.027.2844751
GCTATTG70700.027.0522798
Produced by FastQC (version 0.11.5)