##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146672_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6103222 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45159589475854 31.0 31.0 34.0 28.0 34.0 2 31.582466441495985 33.0 31.0 34.0 28.0 34.0 3 31.70430864222209 33.0 31.0 34.0 28.0 34.0 4 35.131837249243105 37.0 35.0 37.0 32.0 37.0 5 35.29670492077791 37.0 35.0 37.0 32.0 37.0 6 35.36821518207923 37.0 35.0 37.0 32.0 37.0 7 35.302083227514906 37.0 35.0 37.0 32.0 37.0 8 35.269388037990424 37.0 35.0 37.0 32.0 37.0 9 36.79174147687893 39.0 37.0 39.0 32.0 39.0 10-11 36.63218288635085 39.0 37.0 39.0 32.0 39.0 12-13 36.554757716497946 39.0 37.0 39.0 32.0 39.0 14-15 37.75090501377797 40.0 38.0 41.0 32.0 41.0 16-17 37.7800610071205 40.0 38.0 41.0 32.0 41.0 18-19 37.75937676853308 40.0 38.0 41.0 32.0 41.0 20-21 37.827028248358005 40.0 38.0 41.0 32.0 41.0 22-23 37.76531068343901 40.0 37.5 41.0 31.5 41.0 24-25 37.73046154965361 40.0 37.0 41.0 31.5 41.0 26-27 37.76599835300109 40.0 37.0 41.0 32.0 41.0 28-29 37.75550979466256 40.0 37.0 41.0 32.0 41.0 30-31 37.62526129641032 40.0 37.0 41.0 31.0 41.0 32-33 37.61888966188678 40.0 37.0 41.0 31.0 41.0 34-35 37.557513228258784 40.0 37.0 41.0 31.0 41.0 36-37 37.445572027365216 40.0 36.5 41.0 31.0 41.0 38-39 37.34203163509373 40.0 36.0 41.0 30.0 41.0 40-41 37.25512344135606 40.0 36.0 41.0 30.0 41.0 42-43 37.09491781554071 40.0 36.0 41.0 30.0 41.0 44-45 36.94945292830574 39.5 35.0 41.0 30.0 41.0 46-47 36.94050273773426 39.0 35.0 41.0 30.0 41.0 48-49 36.798285970918315 39.0 35.0 41.0 30.0 41.0 50-51 36.61513574305506 39.0 35.0 41.0 30.0 41.0 52-53 36.39909805017743 39.0 35.0 41.0 29.0 41.0 54-55 36.18370591795612 38.0 35.0 40.5 29.0 41.0 56-57 35.939857914393414 38.0 34.5 40.0 29.0 41.0 58-59 35.63669943515081 37.0 34.0 40.0 28.0 41.0 60-61 35.38485540915929 37.0 34.0 40.0 28.0 41.0 62-63 35.07535396877256 36.0 34.0 40.0 28.0 41.0 64-65 34.914414140596556 36.0 34.0 39.0 28.0 41.0 66-67 34.657848428256415 35.0 33.5 39.0 28.0 41.0 68-69 34.52873220079493 35.0 34.0 39.0 28.0 41.0 70-71 34.440637830313236 35.0 34.0 38.5 28.0 41.0 72-73 34.216258068279345 35.0 33.5 37.5 28.0 40.5 74-75 33.95832021184876 35.0 33.0 37.0 27.5 40.0 76-77 33.69822841115726 35.0 33.0 36.5 27.0 39.0 78-79 33.443217697144235 35.0 33.0 36.0 27.0 39.0 80-81 33.2524570628432 35.0 33.0 36.0 27.0 38.5 82-83 32.99298182173285 35.0 33.0 35.5 26.0 37.0 84-85 32.815748960139416 35.0 33.0 35.0 26.0 37.0 86-87 32.642095191687275 35.0 33.0 35.0 26.0 36.5 88-89 32.51399908114108 35.0 33.0 35.0 26.0 36.0 90-91 32.36560483954213 35.0 33.0 35.0 25.5 36.0 92-93 32.213474128910924 35.0 32.5 35.0 25.0 36.0 94-95 32.14672110894868 35.0 32.0 35.0 25.0 35.5 96-97 32.04027176137457 35.0 32.0 35.0 25.0 35.0 98-99 31.950282408209958 35.0 32.0 35.0 25.0 35.0 100 31.01589734078164 34.0 30.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34971061656059277 1101 2 0.46627728498814136 1101 3 0.5079965333224479 1101 4 0.5705241857593464 1101 5 0.48867074455505843 1101 6 0.46638133125340175 1101 7 0.4786584735202055 1101 8 0.4942902605139281 1101 9 0.7050880799387897 1101 10-11 0.7185465507775533 1101 12-13 0.6323293160979162 1101 14-15 0.8626733780451659 1101 16-17 0.7943772666874906 1101 18-19 0.8108487596974356 1101 20-21 0.7405469373988609 1101 22-23 0.797497070217986 1101 24-25 0.8423916288037532 1101 26-27 0.783527724117377 1101 28-29 0.7882107377683951 1101 30-31 0.800348198110072 1101 32-33 0.744166233685398 1101 34-35 0.7937722004004186 1101 36-37 0.836271342090825 1101 38-39 0.7746531605908658 1101 40-41 0.7573259791630278 1101 42-43 0.7399873948347562 1101 44-45 0.7680872132829606 1101 46-47 0.7195773103612098 1101 48-49 0.7116649956163172 1101 50-51 0.6760027182685633 1101 52-53 0.677463989226851 1101 54-55 0.6248836611345467 1101 56-57 0.602135520099047 1101 58-59 0.6023055489822013 1101 60-61 0.5994764457379276 1101 62-63 0.609005925013399 1101 64-65 0.6674144219017961 1101 66-67 0.6899579359022638 1101 68-69 0.6610566665331632 1101 70-71 0.6504929640336528 1101 72-73 0.6460308924912397 1101 74-75 0.5948533418439865 1101 76-77 0.6432488682404269 1101 78-79 0.589678470303511 1101 80-81 0.603643086609047 1101 82-83 0.5966376252155641 1101 84-85 0.5930803059334124 1101 86-87 0.5986226858329573 1101 88-89 0.6377563915944364 1101 90-91 0.5860281811460126 1101 92-93 0.5924120835371838 1101 94-95 0.585576823266102 1101 96-97 0.6100894717444731 1101 98-99 0.5665468953450379 1101 100 0.693702307960173 1102 1 0.35920648914480324 1102 2 0.4375860829623299 1102 3 0.46594601824332926 1102 4 0.5398414178541415 1102 5 0.4591458684013361 1102 6 0.4562263632616492 1102 7 0.4522806750545527 1102 8 0.4961954047206092 1102 9 0.6282483544096564 1102 10-11 0.637185628089604 1102 12-13 0.6228050825287497 1102 14-15 0.7323516028379018 1102 16-17 0.751290931089386 1102 18-19 0.7505730145671023 1102 20-21 0.6926833128808454 1102 22-23 0.7732123818576113 1102 24-25 0.8057926206052457 1102 26-27 0.7581049272800016 1102 28-29 0.7161364262362611 1102 30-31 0.7884560206250484 1102 32-33 0.7291106907787892 1102 34-35 0.7571210317890049 1102 36-37 0.7951343035964413 1102 38-39 0.7840366056691224 1102 40-41 0.7102353402316908 1102 42-43 0.7655960503297052 1102 44-45 0.7811678499097283 1102 46-47 0.7043768140638065 1102 48-49 0.7215893818588199 1102 50-51 0.6884586447029406 1102 52-53 0.6889958780803482 1102 54-55 0.6548808319775503 1102 56-57 0.651714761087689 1102 58-59 0.6348379786777016 1102 60-61 0.5875298518525582 1102 62-63 0.6136285176516836 1102 64-65 0.6752392771516895 1102 66-67 0.7070650142884531 1102 68-69 0.5984022925354608 1102 70-71 0.5741700579568985 1102 72-73 0.5563689028749295 1102 74-75 0.557693361296046 1102 76-77 0.5668386480124212 1102 78-79 0.5321925387403539 1102 80-81 0.6014637531596208 1102 82-83 0.5879987551568746 1102 84-85 0.5678183039810563 1102 86-87 0.5134422524533093 1102 88-89 0.579851838010228 1102 90-91 0.5580981145750386 1102 92-93 0.5633546678139396 1102 94-95 0.5725115818942825 1102 96-97 0.5760876752843203 1102 98-99 0.6038654643727064 1102 100 0.7403495337996873 1103 1 0.28304736204209746 1103 2 0.3358699940178802 1103 3 0.322710140352477 1103 4 0.39152578426401163 1103 5 0.27329489161440534 1103 6 0.3484650086932035 1103 7 0.2910147102716252 1103 8 0.3380979248541678 1103 9 0.4325279008260736 1103 10-11 0.5247278596081983 1103 12-13 0.4953636160909056 1103 14-15 0.5742907112181044 1103 16-17 0.5357146514166544 1103 18-19 0.5994192874507291 1103 20-21 0.506692470723408 1103 22-23 0.5783839993386053 1103 24-25 0.6048148843863785 1103 26-27 0.5214223573673493 1103 28-29 0.5702322061564757 1103 30-31 0.5206492556061164 1103 32-33 0.53900629454116 1103 34-35 0.544841677520516 1103 36-37 0.5288648889282399 1103 38-39 0.6101576418787928 1103 40-41 0.5424890817734607 1103 42-43 0.560525606267646 1103 44-45 0.4704736253763855 1103 46-47 0.5179719332012382 1103 48-49 0.514203884466653 1103 50-51 0.40435620448155163 1103 52-53 0.4067906123876597 1103 54-55 0.434110023044326 1103 56-57 0.4718618715988896 1103 58-59 0.48522293759773305 1103 60-61 0.4848468218916011 1103 62-63 0.4006046378817061 1103 64-65 0.3667977580496711 1103 66-67 0.36741205118429576 1103 68-69 0.3987657039835071 1103 70-71 0.4259100461341774 1103 72-73 0.39190812108665796 1103 74-75 0.36512603224377926 1103 76-77 0.3252009466434984 1103 78-79 0.3430629068209754 1103 80-81 0.33354514831764703 1103 82-83 0.4072826311029729 1103 84-85 0.402363199620126 1103 86-87 0.38379060786170527 1103 88-89 0.4297667309817328 1103 90-91 0.4633409718051311 1103 92-93 0.4009234365445451 1103 94-95 0.36467531444116474 1103 96-97 0.46505331839598085 1103 98-99 0.4345240871586604 1103 100 0.4993821081200984 1104 1 0.2441443170084483 1104 2 0.36745484571041587 1104 3 0.29676373906707454 1104 4 0.46201690038634524 1104 5 0.33444497581541555 1104 6 0.3371228461199536 1104 7 0.33841346578184783 1104 8 0.38770891142063846 1104 9 0.43448248820802604 1104 10-11 0.5667100730883803 1104 12-13 0.4410840681971351 1104 14-15 0.6154113682987017 1104 16-17 0.5862433038583674 1104 18-19 0.5407178759570783 1104 20-21 0.5084621267925513 1104 22-23 0.482939412688836 1104 24-25 0.559608836594343 1104 26-27 0.5082374354367829 1104 28-29 0.46098855177024234 1104 30-31 0.4455746162060805 1104 32-33 0.4913056286596529 1104 34-35 0.5477263714442486 1104 36-37 0.47368839427299747 1104 38-39 0.4863351360185746 1104 40-41 0.5389462972864578 1104 42-43 0.5718149247527577 1104 44-45 0.4916299331300493 1104 46-47 0.47103702368835343 1104 48-49 0.42975238586534203 1104 50-51 0.37709605961239845 1104 52-53 0.4460609215343112 1104 54-55 0.5095211583285106 1104 56-57 0.5333329295954883 1104 58-59 0.46395282419037187 1104 60-61 0.47108063798717836 1104 62-63 0.4964513960969654 1104 64-65 0.453276924325408 1104 66-67 0.40755293103973855 1104 68-69 0.43947552012471647 1104 70-71 0.4219343678442016 1104 72-73 0.36504207629694463 1104 74-75 0.2870817068617981 1104 76-77 0.2904013899267497 1104 78-79 0.26864240272608697 1104 80-81 0.2836607890271452 1104 82-83 0.3328098578121086 1104 84-85 0.35433992724927776 1104 86-87 0.35767930450666086 1104 88-89 0.32167042338995344 1104 90-91 0.35177148767012767 1104 92-93 0.36157034292560297 1104 94-95 0.4336003783047886 1104 96-97 0.41815366127087117 1104 98-99 0.354757936735961 1104 100 0.4169951852704443 1105 1 0.20409497683350963 1105 2 0.3107006879452996 1105 3 0.3034712775395789 1105 4 0.41328437585436717 1105 5 0.31110464637122703 1105 6 0.2746874265860342 1105 7 0.32966753961759565 1105 8 0.3637944545057792 1105 9 0.4621945105898959 1105 10-11 0.49271628927508004 1105 12-13 0.336329294151291 1105 14-15 0.49898544225691666 1105 16-17 0.4943130727923659 1105 18-19 0.4661557607470641 1105 20-21 0.44144709334082677 1105 22-23 0.5087102827592958 1105 24-25 0.49062412527299415 1105 26-27 0.45290048708380937 1105 28-29 0.519308242975832 1105 30-31 0.529764060167679 1105 32-33 0.4535725050188475 1105 34-35 0.5083484551316175 1105 36-37 0.4762482844118239 1105 38-39 0.4711534878374479 1105 40-41 0.49885535849464446 1105 42-43 0.49329523915577056 1105 44-45 0.4986571042816692 1105 46-47 0.5098902372336411 1105 48-49 0.3437355479432682 1105 50-51 0.42021894410088123 1105 52-53 0.38445760745348423 1105 54-55 0.38150824815893714 1105 56-57 0.37812397519480356 1105 58-59 0.43376983841957895 1105 60-61 0.47314751795824606 1105 62-63 0.46638941792942745 1105 64-65 0.4220325137928924 1105 66-67 0.34423238168241 1105 68-69 0.3624131450065349 1105 70-71 0.3815687783443238 1105 72-73 0.37215082510950026 1105 74-75 0.3661810497722655 1105 76-77 0.4100143912272429 1105 78-79 0.4041662038572795 1105 80-81 0.32100679593930437 1105 82-83 0.39251936435400836 1105 84-85 0.3594488308752375 1105 86-87 0.3270606092762307 1105 88-89 0.3778739097578381 1105 90-91 0.3567906935851468 1105 92-93 0.3637523456822578 1105 94-95 0.4126618531623407 1105 96-97 0.3443851811130543 1105 98-99 0.38991360452865464 1105 100 0.5303863036307348 1106 1 0.12289899448314046 1106 2 0.18825683633351886 1106 3 0.18044376523692662 1106 4 0.3150427376460101 1106 5 0.17296279234154355 1106 6 0.25480919205975283 1106 7 0.23156056781466816 1106 8 0.20205139499519476 1106 9 0.31350910150364797 1106 10-11 0.3697017818995789 1106 12-13 0.27627462376809575 1106 14-15 0.4226318989479907 1106 16-17 0.31413550705682525 1106 18-19 0.3048636358174903 1106 20-21 0.2852355086506648 1106 22-23 0.30134181635691704 1106 24-25 0.3358601934479637 1106 26-27 0.37366007530714285 1106 28-29 0.35517161727864277 1106 30-31 0.40323175297696423 1106 32-33 0.288719833309365 1106 34-35 0.28903693641478867 1106 36-37 0.3526762306349198 1106 38-39 0.3558696566215289 1106 40-41 0.2912168557181616 1106 42-43 0.21056629799417692 1106 44-45 0.24465097384915424 1106 46-47 0.2980736185351489 1106 48-49 0.20891619446140197 1106 50-51 0.21380972024581268 1106 52-53 0.20222089344041905 1106 54-55 0.32743364013408893 1106 56-57 0.31086880204529166 1106 58-59 0.283755074034616 1106 60-61 0.29398917903460386 1106 62-63 0.3499072041131015 1106 64-65 0.276764333451645 1106 66-67 0.273151242512796 1106 68-69 0.2692654806985999 1106 70-71 0.295640415133569 1106 72-73 0.24900612005460232 1106 74-75 0.2741397441888296 1106 76-77 0.24724303003801396 1106 78-79 0.20415771822476358 1106 80-81 0.18954096574780266 1106 82-83 0.1604840964426728 1106 84-85 0.1657208126237606 1106 86-87 0.1423970527329388 1106 88-89 0.2367730060670965 1106 90-91 0.22107835342743698 1106 92-93 0.18283174019087056 1106 94-95 0.22249332634586239 1106 96-97 0.26475767212814105 1106 98-99 0.2505339023968922 1106 100 0.3113479323568171 1107 1 0.11573500612278309 1107 2 0.13110328147356043 1107 3 0.09840977455357347 1107 4 0.2144892809723089 1107 5 0.1390134269194192 1107 6 0.1859038011412366 1107 7 0.12412181299600888 1107 8 0.14168717889005222 1107 9 0.13721057903793366 1107 10-11 0.2616029191961715 1107 12-13 0.20080744163522013 1107 14-15 0.2417673669898548 1107 16-17 0.15712236195881246 1107 18-19 0.11892859621385554 1107 20-21 0.13155068188609675 1107 22-23 0.2612774918828791 1107 24-25 0.2781723646031651 1107 26-27 0.1594477298278889 1107 28-29 0.19502894493173528 1107 30-31 0.24544654442427571 1107 32-33 0.22396713352363662 1107 34-35 0.24886893886912276 1107 36-37 0.2738947419975233 1107 38-39 0.25112452155467224 1107 40-41 0.17892870091560553 1107 42-43 0.23614880487713208 1107 44-45 0.28032970155773995 1107 46-47 0.18663011168730748 1107 48-49 0.22366536598072173 1107 50-51 0.1637755873415614 1107 52-53 0.2149646268126375 1107 54-55 0.2396790240930784 1107 56-57 0.19194626040344076 1107 58-59 0.16018572821391786 1107 60-61 0.15897492240195277 1107 62-63 0.22295789254668108 1107 64-65 0.15391103714469523 1107 66-67 0.1826943515370516 1107 68-69 0.15320424463412508 1107 70-71 0.16526345644091833 1107 72-73 0.13585398219716183 1107 74-75 0.18969422819269255 1107 76-77 0.16463578367189058 1107 78-79 0.17474606051438002 1107 80-81 0.10480226933605508 1107 82-83 0.05630697722418887 1107 84-85 0.09895189464944565 1107 86-87 0.11821924977726184 1107 88-89 0.13650998195531372 1107 90-91 0.11984737837891402 1107 92-93 0.13075708116141982 1107 94-95 0.09124369047820124 1107 96-97 0.17950516601046473 1107 98-99 0.13750214166913466 1107 100 0.14630569778972458 1108 1 5.055734137506818E-4 1108 2 0.025982073674491346 1108 3 0.008631384379480522 1108 4 0.11128411250444259 1108 5 0.0551830013781327 1108 6 0.04019734703366851 1108 7 0.064169827658624 1108 8 0.07339515395627672 1108 9 0.1159721165365184 1108 10-11 0.2165884587482978 1108 12-13 0.0775290211466384 1108 14-15 0.12195798188547968 1108 16-17 0.19682366282118835 1108 18-19 0.15037014180793307 1108 20-21 0.11621285306657114 1108 22-23 0.1699820904819589 1108 24-25 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-0.9430134622361024 2215 42-43 -0.9106817353433811 2215 44-45 -0.9569161821582242 2215 46-47 -1.0629853129874647 2215 48-49 -1.1085430781607428 2215 50-51 -0.9911160119208944 2215 52-53 -0.986684186456813 2215 54-55 -0.938792519691475 2215 56-57 -0.9856706060301264 2215 58-59 -1.0033041535566056 2215 60-61 -0.9707722021346257 2215 62-63 -0.9533310881031625 2215 64-65 -0.9453168960954841 2215 66-67 -0.8993019697695317 2215 68-69 -0.8677600033824788 2215 70-71 -0.9408241738251419 2215 72-73 -0.9268196676937848 2215 74-75 -1.0280363600739477 2215 76-77 -1.0025275167914245 2215 78-79 -0.9715726864291412 2215 80-81 -0.9233358221657753 2215 82-83 -0.8969756640504585 2215 84-85 -0.8527351523518583 2215 86-87 -0.8433232820264251 2215 88-89 -0.8753884626058905 2215 90-91 -0.9076819324066712 2215 92-93 -0.8884129983985147 2215 94-95 -0.9105505476649043 2215 96-97 -1.1620502770818995 2215 98-99 -1.0237020195975042 2215 100 -0.5716337300085073 2216 1 -0.4510877312057957 2216 2 -0.720557360936553 2216 3 -0.6583737716010845 2216 4 -0.46885636457798086 2216 5 -0.4521724311935671 2216 6 -0.3518657989192704 2216 7 -0.5180608927692489 2216 8 -0.5600481880636394 2216 9 -0.6565523611962689 2216 10-11 -1.0080547721939581 2216 12-13 -0.6413204876237586 2216 14-15 -0.7537974095070084 2216 16-17 -0.7398444496797723 2216 18-19 -0.9746609877158008 2216 20-21 -1.0209644334521002 2216 22-23 -0.9364550824180355 2216 24-25 -0.9970618301361895 2216 26-27 -1.3504677523629454 2216 28-29 -1.2033464727110612 2216 30-31 -1.3243261624153604 2216 32-33 -1.5781832917024516 2216 34-35 -1.1828323066896544 2216 36-37 -1.2577608407760366 2216 38-39 -1.1582336906433284 2216 40-41 -1.0630179536786386 2216 42-43 -1.0607561232914549 2216 44-45 -1.063939300134102 2216 46-47 -1.1379138056174796 2216 48-49 -1.1368295366020718 2216 50-51 -1.2164509560015375 2216 52-53 -1.2593271091940181 2216 54-55 -1.2303147737951576 2216 56-57 -1.1658491913281424 2216 58-59 -1.1453528568395939 2216 60-61 -1.159061305436147 2216 62-63 -1.2536351666954744 2216 64-65 -1.2044924548159202 2216 66-67 -1.2481142854596001 2216 68-69 -1.168933085232517 2216 70-71 -1.0749467010402682 2216 72-73 -1.0012165273591052 2216 74-75 -1.0651979310586484 2216 76-77 -1.0654118141490088 2216 78-79 -0.987670446245609 2216 80-81 -1.1242487959731804 2216 82-83 -1.0877285741121057 2216 84-85 -1.0438303027034763 2216 86-87 -1.003389774052657 2216 88-89 -1.042926217759213 2216 90-91 -1.1445858253265868 2216 92-93 -1.0550267745554862 2216 94-95 -1.0439163426238736 2216 96-97 -1.0947373501068007 2216 98-99 -1.0824753015090245 2216 100 -0.8470543985867884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 2.0 2 17.0 3 28.0 4 53.0 5 37.0 6 68.0 7 197.0 8 598.0 9 1009.0 10 1420.0 11 2455.0 12 4040.0 13 6221.0 14 8889.0 15 12324.0 16 15341.0 17 18137.0 18 20994.0 19 23839.0 20 27483.0 21 31734.0 22 37017.0 23 43237.0 24 50907.0 25 59319.0 26 66899.0 27 74580.0 28 85419.0 29 104396.0 30 122406.0 31 143513.0 32 177307.0 33 231994.0 34 337164.0 35 487410.0 36 781896.0 37 1402431.0 38 1238849.0 39 482662.0 40 930.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.843652400586926 21.03242042445754 12.174422703314178 23.94950447164135 2 15.705155410164002 19.829195146977906 40.72220508970212 23.74344435315597 3 18.525534190467592 26.952242263678656 28.947592220476743 25.574631325377016 4 11.970949009192564 16.642739898689896 40.73919375012392 30.647117341993617 5 12.892336379381252 38.88903635758866 35.32008081284747 12.898546450182616 6 29.534691328146977 38.94500912410105 18.298652136704384 13.221647411047593 7 26.591635725100936 31.950522113832303 24.128194998960375 17.329647162106387 8 26.087491528934017 35.151925373349535 20.03733167647803 18.72325142123842 9 26.1749183623646 14.867929721997841 21.672993958896907 37.28415795674066 10-11 25.654472994709494 25.842800645498183 27.833479950576688 20.669246409215635 12-13 25.73565458405126 23.827787830785272 27.73428513941202 22.702272445751444 14-15 23.137597312496872 25.080426919499676 25.080082838354855 26.701892929648597 16-17 22.067185837703587 27.536193771422194 26.434294781205438 23.962325609668778 18-19 21.339653266097823 28.46085054146041 28.036928300981597 22.162567891460178 20-21 23.300721838587027 27.53499209016961 26.871082462951602 22.293203608291766 22-23 23.796021020953365 27.17903718056344 26.504196973086923 22.520744825396267 24-25 23.170917537229187 27.19735998277609 27.382805446768238 22.248917033226494 26-27 23.076705025610725 27.4812451135788 26.266950756016357 23.17509910479412 28-29 22.098466650718173 28.08475531625534 26.89720431657977 22.91957371644672 30-31 22.968326648949972 27.645294909892527 26.92601467841593 22.460363762741572 32-33 23.430987675925945 27.832691993841568 25.870486454529782 22.865833875702705 34-35 23.312346136285452 27.612505410243294 26.97293538848435 22.102213064986902 36-37 22.93807832050539 27.178493166869117 26.57643109361719 23.306997419008297 38-39 23.140446642480043 27.26880658344507 27.234076754315172 22.356670019759708 40-41 22.75301781580087 26.348023813827037 27.941781540761095 22.957176829610994 42-43 22.71957296178448 26.651198209989623 27.175220994144677 23.45400783408122 44-45 22.92151004149802 27.64460966591489 27.017075446683027 22.416804845904064 46-47 22.80169824226976 27.366392147981163 26.51993404209782 23.31197556765126 48-49 23.576235683446942 26.90048905287069 26.843441215366404 22.67983404831596 50-51 23.100838001209258 26.721538124831053 26.84148903664726 23.33613483731243 52-53 22.480857730985566 27.419825359573103 26.65771557997142 23.441601329469915 54-55 22.55964818265397 27.271047503863464 26.589364522953833 23.579939790528726 56-57 23.69577203329357 26.647711392804585 26.358695496315747 23.297821077586097 58-59 22.480968361680894 27.1124489656623 27.36177127324891 23.044811399407898 60-61 22.555206520640102 27.79649317961667 26.524628811500335 23.12367148824289 62-63 23.073838584170684 26.99777156428191 26.124652129533416 23.80373772201399 64-65 22.104641754694295 27.206036882500783 26.729616406424427 23.95970495638049 66-67 22.995571793360188 26.808868539740583 26.573213712897026 23.622345954002203 68-69 22.259354044478822 26.918128756063837 26.947180606406988 23.87533659305035 70-71 22.300974746837916 26.548310110269103 27.097849028657418 24.05286611423557 72-73 22.925498506148852 26.673972742808367 27.528949152364625 22.871579598678153 74-75 23.161784255486747 26.93577001755264 26.968289474126223 22.93415625283439 76-77 22.80647419993016 26.87542164674798 26.619955130313322 23.698149023008536 78-79 22.71881449513732 26.8364171648207 26.661921575223968 23.78284676481801 80-81 22.248735635461216 27.22274536234438 27.428003096727338 23.100515905467063 82-83 23.124976598896524 26.159611215193397 28.478083990974323 22.23732819493576 84-85 23.394465383307924 26.722432384667766 27.015883266589423 22.867218965434883 86-87 21.942383474434802 27.44476305875087 27.517067813994974 23.095785652819355 88-89 23.228679631006823 26.091282821767265 27.29405906175031 23.385978485475604 90-91 22.79635447631321 26.184364219384282 27.440798585733976 23.578482718568534 92-93 23.116693802321343 26.397125718046127 27.41896500418177 23.06721547545075 94-95 21.942592250457682 26.439239047658454 28.081409234119548 23.536759467764316 96-97 22.336642976032024 26.360519596288317 27.550524385213077 23.75231304246658 98-99 21.6236276219608 27.157055220554653 27.43069102750172 23.788626129982827 100 22.5569826158852 26.337780761018898 26.538459579793404 24.5667770433025 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 521.5 2 296.0 3 336.5 4 311.0 5 287.5 6 268.0 7 284.5 8 299.0 9 312.0 10 323.5 11 300.5 12 287.5 13 309.5 14 354.5 15 537.5 16 665.5 17 772.5 18 1053.5 19 1338.0 20 1741.5 21 2271.5 22 3167.0 23 4732.5 24 6641.5 25 8705.0 26 11883.0 27 15999.5 28 21950.0 29 33901.5 30 50528.0 31 66284.0 32 87410.5 33 108268.5 34 123523.5 35 142084.5 36 159245.5 37 188163.0 38 199247.5 39 185174.0 40 173560.5 41 160743.0 42 159390.0 43 170979.0 44 187905.5 45 225438.5 46 262232.0 47 285179.5 48 300107.0 49 293925.5 50 278075.5 51 281979.5 52 283660.0 53 268338.5 54 252077.5 55 215607.0 56 187070.5 57 152997.0 58 118550.0 59 114034.0 60 93553.5 61 62189.5 62 45380.0 63 38485.5 64 27968.0 65 12269.5 66 5942.0 67 3959.5 68 2925.5 69 2186.0 70 1599.0 71 1167.0 72 863.0 73 726.0 74 540.5 75 407.0 76 314.5 77 186.0 78 104.5 79 63.5 80 44.5 81 36.0 82 31.5 83 31.5 84 28.0 85 19.0 86 18.0 87 18.5 88 15.5 89 13.0 90 11.0 91 10.0 92 9.5 93 7.5 94 7.0 95 9.5 96 7.5 97 6.0 98 5.0 99 4.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06016494238616914 2 0.00766808089235489 3 0.007913852715827804 4 0.009388483656665282 5 0.0038012708697143897 6 0.0025396421758867695 7 8.356241998079048E-4 8 0.0015401700937635893 9 5.243132234088814E-4 10-11 4.751588587142987E-4 12-13 2.949261881674958E-4 14-15 1.7204027643103923E-4 16-17 5.079284351773538E-4 18-19 0.0016057092466896991 20-21 9.3393292919707E-4 22-23 0.0012042819350172745 24-25 0.0013107830585222034 26-27 0.003481767499199603 28-29 0.003965118752029666 30-31 0.00516940068704694 32-33 0.006062371645665191 34-35 0.0035309218638941856 36-37 0.008667552974478071 38-39 0.00653753050437949 40-41 0.006242604316211995 42-43 0.014926542078921592 44-45 0.020382676560020265 46-47 0.01880973688979362 48-49 0.021718036800889757 50-51 0.018719620554520217 52-53 0.025265343453015475 54-55 0.019530667571980832 56-57 0.01577035867284526 58-59 0.01770376368416551 60-61 0.016368403443296015 62-63 0.012042819350172745 64-65 0.01203462695605698 66-67 0.014164649426155562 68-69 0.005579020392835129 70-71 0.009183673803771188 72-73 0.008831400856793346 74-75 0.011821624709047124 76-77 0.010830345021039706 78-79 0.01270640327354961 80-81 0.010658304744608667 82-83 0.006742340357273584 84-85 0.010936846144544637 86-87 0.00951136956840174 88-89 0.008315280027500229 90-91 0.013664913385093971 92-93 0.009011633527340149 94-95 0.007774582015859819 96-97 0.009822680544800762 98-99 0.009585101115443613 100 0.26023959148135195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6103222.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.45959906050302 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0413813258829 45.71717174705338 2 15.866684254507346 20.137868424212648 3 5.9383683353609635 11.305394209067803 4 2.6492023318203444 6.724692712298749 5 1.3387030609031636 4.247677975299146 6 0.7472199877200464 2.845096849842488 7 0.44718705104017437 1.9864817674840711 8 0.28775558555183434 1.4608683269231746 9 0.18916769145594342 1.0804055269495598 >10 0.49148289213975743 4.321745138236866 >50 0.0024816892952301034 0.10043824274434307 >100 3.258965681313784E-4 0.043042281853753146 >500 0.0 0.0 >1k 3.989775414132225E-5 0.02911679803406259 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.276957646305509E-5 0.0 24-25 0.0 0.0 0.0 1.146935176206928E-4 0.0 26-27 0.0 0.0 0.0 3.5227294697784216E-4 0.0 28-29 0.0 0.0 0.0 0.0027526444228966273 0.0 30-31 0.0 0.0 0.0 0.010240492644704714 0.0 32-33 0.0 0.0 0.0 0.024560797559059787 0.0 34-35 0.0 0.0 0.0 0.047220959683262384 0.0 36-37 0.0 0.0 0.0 0.08682299283886447 0.0 38-39 0.0 0.0 0.0 0.1445875637491148 0.0 40-41 0.0 0.0 0.0 0.2070299916994663 0.0 42-43 0.0 0.0 0.0 0.27199567703747296 0.0 44-45 0.0 0.0 0.0 0.3536574615834063 0.0 46-47 0.0 0.0 0.0 0.45413717541324894 0.0 48-49 0.0 0.0 0.0 0.5720011495567423 0.0 50-51 0.0 0.0 0.0 0.6954113745166077 0.0 52-53 0.0 0.0 0.0 0.8178385121825815 0.0 54-55 0.0 0.0 0.0 0.9552167691098243 0.0 56-57 0.0 0.0 0.0 1.1239555107122108 0.0 58-59 0.0 0.0 0.0 1.3308790012881722 0.0 60-61 0.0 0.0 0.0 1.5449216823507321 0.0 62-63 0.0 0.0 0.0 1.7688443907169034 0.0 64-65 0.0 0.0 0.0 2.0072676366679763 0.0 66-67 0.0 0.0 0.0 2.2743642620242226 0.0 68-69 0.0 0.0 0.0 2.577876079225039 0.0 70-71 0.0 0.0 0.0 2.9161809942355035 0.0 72-73 0.0 0.0 0.0 3.2726730241829642 1.6384788231527545E-5 74-75 0.0 0.0 0.0 3.621365894932218 1.6384788231527545E-5 76-77 0.0 0.0 0.0 4.020056946969977 1.6384788231527545E-5 78-79 0.0 0.0 0.0 4.456211817299125 1.6384788231527545E-5 80-81 0.0 0.0 0.0 4.930756246454742 1.6384788231527545E-5 82-83 0.0 0.0 0.0 5.427543025634657 1.6384788231527545E-5 84-85 0.0 0.0 0.0 5.915785137751831 1.6384788231527545E-5 86-87 0.0 0.0 0.0 6.4350272692030535 1.6384788231527545E-5 88 0.0 0.0 0.0 6.854248460894918 1.6384788231527545E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 6330 0.0 41.832348 94 GTACTAG 7155 0.0 34.172382 1 CAACGCA 10770 0.0 32.770653 5 GCATCGT 7025 0.0 31.573631 8 TAGGCAT 8295 0.0 31.387323 5 TACCCCG 485 0.0 31.007668 5 CTGTGCG 9335 0.0 30.60631 9 GTATCAA 13160 0.0 30.477175 1 ATCAACG 11630 0.0 30.348618 3 TAGTACT 9885 0.0 29.572554 4 TCTAACG 3690 0.0 28.402538 2 TATTCTC 3430 0.0 28.362017 5 ACTGTGC 10505 0.0 28.3163 8 CTAACGC 3720 0.0 28.173487 3 TAACGCC 3765 0.0 27.836523 4 GTACTGT 10580 0.0 27.67163 6 CTAGGCA 9740 0.0 27.551945 4 CGCCTAC 3810 0.0 27.381474 7 GGTATCA 8720 0.0 27.284475 1 GCTATTG 7070 0.0 27.052279 8 >>END_MODULE