FastQCFastQC Report
Mon 16 Jan 2017
ERR1146672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103222
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146430.23992245407425786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127030.20813596490509437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77480.1269493392178754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119850.043.570491
GGGCACC64050.042.2891894
GTATCAA207550.038.510411
GTGGTAT26900.038.4749371
CAACGCA234250.032.7442555
ATCAACG236700.032.4737783
GTACTAG74250.031.8697781
CTGTGCG92950.030.7980339
TACCCCG4350.030.2526445
TATCAAC265700.030.0644742
TAGGCAT87550.029.7404965
TAGTACT99850.029.20944
ACTGTGC103050.029.1431678
AACGCAG277400.028.869076
GTACTGT104750.028.1757356
CTAACGC30950.027.8143843
TCTAACG32100.027.2593462
TACTGTG117650.026.8839327
ACGCAGA286650.026.7406317
TAACGCC32400.026.7147944
Produced by FastQC (version 0.11.5)