FastQCFastQC Report
Mon 16 Jan 2017
ERR1146671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691569
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59150.041.73164494
GTACTAG66550.034.6224171
TAGGCAT80050.031.9356655
CTGTGCG83250.031.5531799
TAGTACT87250.031.132654
GCATCGT68250.031.052268
GGTATCA83150.030.9338871
TACCCCG3850.030.5153055
GTATCAA122950.030.4434571
CAACGCA111100.030.0318935
ACTGTGC94500.029.6866978
GTACTGT93750.029.1224586
TATTCTC32200.029.0426085
CTAGGCA91150.029.0272184
ATCAACG116850.028.8366053
TACTGTG101950.028.6699097
CAGTACG7900.027.9567249
CTAGTAC101900.027.9019723
CGCCTAC31400.026.9380427
AGGGCTA70900.026.512595
Produced by FastQC (version 0.11.5)