##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146671_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5691569 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.367496730690604 31.0 31.0 34.0 28.0 34.0 2 31.435763670790955 31.0 31.0 34.0 28.0 34.0 3 31.547285467328955 33.0 31.0 34.0 28.0 34.0 4 34.97694431886884 37.0 35.0 37.0 32.0 37.0 5 35.12305815847967 37.0 35.0 37.0 32.0 37.0 6 35.24059745212612 37.0 35.0 37.0 32.0 37.0 7 35.146375630340245 37.0 35.0 37.0 32.0 37.0 8 35.13841209691036 37.0 35.0 37.0 32.0 37.0 9 36.62285742297071 39.0 37.0 39.0 32.0 39.0 10-11 36.45031422793961 39.0 37.0 39.0 31.5 39.0 12-13 36.379809855595184 39.0 37.0 39.0 31.5 39.0 14-15 37.54592072941574 40.0 37.0 41.0 31.0 41.0 16-17 37.577897149274655 40.0 37.0 41.0 31.0 41.0 18-19 37.550521481862035 40.0 37.0 41.0 31.0 41.0 20-21 37.634033251639394 40.0 37.0 41.0 31.0 41.0 22-23 37.574099426713445 40.0 37.0 41.0 31.0 41.0 24-25 37.54115745587904 40.0 37.0 41.0 31.0 41.0 26-27 37.58593254338127 40.0 37.0 41.0 31.0 41.0 28-29 37.58491726973705 40.0 37.0 41.0 31.0 41.0 30-31 37.46187334283394 40.0 37.0 41.0 31.0 41.0 32-33 37.46148891456819 40.0 37.0 41.0 31.0 41.0 34-35 37.39810481081754 40.0 36.0 41.0 30.5 41.0 36-37 37.27581884714039 40.0 36.0 41.0 30.0 41.0 38-39 37.16885326348499 40.0 36.0 41.0 30.0 41.0 40-41 37.0800075515205 40.0 36.0 41.0 30.0 41.0 42-43 36.911586418437516 39.5 35.5 41.0 30.0 41.0 44-45 36.740727117601494 39.0 35.0 41.0 30.0 41.0 46-47 36.729991677163184 39.0 35.0 41.0 30.0 41.0 48-49 36.58828778145359 39.0 35.0 41.0 29.5 41.0 50-51 36.40225270044165 39.0 35.0 41.0 29.0 41.0 52-53 36.18853579742247 38.5 35.0 40.5 29.0 41.0 54-55 35.97239996563338 38.0 34.5 40.0 28.5 41.0 56-57 35.725190277057166 38.0 34.0 40.0 28.0 41.0 58-59 35.42028973030108 37.0 34.0 40.0 28.0 41.0 60-61 35.17353431013487 37.0 34.0 40.0 28.0 41.0 62-63 34.86654295151302 36.0 33.5 39.5 27.5 41.0 64-65 34.709312230072236 36.0 33.0 39.0 27.0 41.0 66-67 34.45532479708143 35.0 33.0 39.0 27.0 41.0 68-69 34.33361275247651 35.0 33.0 39.0 27.0 41.0 70-71 34.253999029090224 35.0 33.0 38.5 27.5 41.0 72-73 34.03774583071909 35.0 33.0 37.5 27.0 40.0 74-75 33.783042602136604 35.0 33.0 37.0 27.0 39.5 76-77 33.528052633641096 35.0 33.0 36.5 26.5 39.0 78-79 33.279846734705316 35.0 33.0 36.0 26.0 39.0 80-81 33.10042793120843 35.0 33.0 36.0 26.0 38.5 82-83 32.8424626847184 35.0 33.0 35.5 26.0 37.0 84-85 32.66409244972695 35.0 33.0 35.0 26.0 37.0 86-87 32.49560955862962 35.0 33.0 35.0 25.0 36.5 88-89 32.370528056499005 35.0 32.0 35.0 25.0 36.0 90-91 32.21988637930947 35.0 32.0 35.0 25.0 36.0 92-93 32.0682919771332 35.0 32.0 35.0 25.0 36.0 94-95 32.00040068037478 35.0 32.0 35.0 25.0 35.5 96-97 31.892776578830897 35.0 32.0 35.0 25.0 35.0 98-99 31.801062413545367 35.0 32.0 35.0 24.5 35.0 100 30.853632451789657 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46039513351500716 1101 2 0.535374172724417 1101 3 0.5360897032562519 1101 4 0.6204707418105215 1101 5 0.5231844576690037 1101 6 0.5264714271359381 1101 7 0.515062299430241 1101 8 0.5682676999118996 1101 9 0.6855548677737673 1101 10-11 0.7481784242631235 1101 12-13 0.7166741161039027 1101 14-15 0.8756291631623014 1101 16-17 0.7753520616104268 1101 18-19 0.8248854804876302 1101 20-21 0.8219436873903376 1101 22-23 0.839756434939865 1101 24-25 0.8777880016615072 1101 26-27 0.8224810794654474 1101 28-29 0.7907448574112692 1101 30-31 0.8232205800109469 1101 32-33 0.8288946919993876 1101 34-35 0.8399790230774826 1101 36-37 0.823106626586096 1101 38-39 0.792391100398774 1101 40-41 0.7680184373492196 1101 42-43 0.7591351101592991 1101 44-45 0.7717388114689143 1101 46-47 0.7946369055064721 1101 48-49 0.7952187579797112 1101 50-51 0.7192892537042255 1101 52-53 0.7344187303719494 1101 54-55 0.6921131793541093 1101 56-57 0.6789098327952559 1101 58-59 0.6645004913343797 1101 60-61 0.6506319866494579 1101 62-63 0.6575124862835651 1101 64-65 0.6644769155708801 1101 66-67 0.7282538296392573 1101 68-69 0.623129291556701 1101 70-71 0.6144441687421889 1101 72-73 0.6060886474049667 1101 74-75 0.5899133019036711 1101 76-77 0.6170336026658418 1101 78-79 0.6266671665488559 1101 80-81 0.5741038605846214 1101 82-83 0.5682818621662449 1101 84-85 0.560525679031862 1101 86-87 0.5416670228315539 1101 88-89 0.5744359178017078 1101 90-91 0.5531925655019663 1101 92-93 0.5088814799241277 1101 94-95 0.5159794373636188 1101 96-97 0.5565628945753787 1101 98-99 0.5563038973567842 1101 100 0.6130719417712776 1102 1 0.3377066953344645 1102 2 0.42370374659603627 1102 3 0.412483753564441 1102 4 0.5042809542541349 1102 5 0.3711953060581976 1102 6 0.4190236892588999 1102 7 0.4073950260715975 1102 8 0.44337216276796454 1102 9 0.5502074398621417 1102 10-11 0.6436331589828583 1102 12-13 0.5663516324943316 1102 14-15 0.7250023326434274 1102 16-17 0.6478048828348761 1102 18-19 0.682520457441079 1102 20-21 0.6311925103732037 1102 22-23 0.6544692224746242 1102 24-25 0.6683943252976405 1102 26-27 0.5889475751480049 1102 28-29 0.626485674222053 1102 30-31 0.6538927295929469 1102 32-33 0.6737280145227658 1102 34-35 0.723102002717134 1102 36-37 0.7396579870788358 1102 38-39 0.6758362433530749 1102 40-41 0.6155771360656601 1102 42-43 0.5822711204642559 1102 44-45 0.5534783705882802 1102 46-47 0.5203805702729909 1102 48-49 0.5135546016245343 1102 50-51 0.5229500645788718 1102 52-53 0.5008570667921219 1102 54-55 0.46751910646513295 1102 56-57 0.4871245702588425 1102 58-59 0.44352261490575984 1102 60-61 0.4904656388097024 1102 62-63 0.4919545738911708 1102 64-65 0.4682472272738991 1102 66-67 0.48654920135086854 1102 68-69 0.4080002006898269 1102 70-71 0.39218511260330047 1102 72-73 0.404335940178818 1102 74-75 0.3563345559277096 1102 76-77 0.42072857833277055 1102 78-79 0.4529978518438753 1102 80-81 0.4175254164866331 1102 82-83 0.40548055472234523 1102 84-85 0.42714539580039457 1102 86-87 0.3894887637572637 1102 88-89 0.4540028230080182 1102 90-91 0.4419873677558783 1102 92-93 0.4134506071765003 1102 94-95 0.44118049583158836 1102 96-97 0.4488061388213467 1102 98-99 0.41936326909294763 1102 100 0.46933145219514216 1103 1 0.26627837316114267 1103 2 0.28373417596185035 1103 3 0.34407594924297413 1103 4 0.4408403370457208 1103 5 0.31425879807895996 1103 6 0.32392989144595674 1103 7 0.35413024733256293 1103 8 0.33198769698470443 1103 9 0.3883288882151774 1103 10-11 0.5162074870053033 1103 12-13 0.45101380040283345 1103 14-15 0.6218650821484744 1103 16-17 0.5830295322477568 1103 18-19 0.5988055890962016 1103 20-21 0.493427061187397 1103 22-23 0.5998669827922853 1103 24-25 0.5801760982525153 1103 26-27 0.555700984066064 1103 28-29 0.523037096797971 1103 30-31 0.5819973885396266 1103 32-33 0.526879960395128 1103 34-35 0.5375693770778582 1103 36-37 0.5452968971508625 1103 38-39 0.6143580483376709 1103 40-41 0.47674118543113053 1103 42-43 0.5130349839736184 1103 44-45 0.4897784424606755 1103 46-47 0.4962592922396283 1103 48-49 0.5323504842639437 1103 50-51 0.4882386929718123 1103 52-53 0.478517180424312 1103 54-55 0.4802540841966021 1103 56-57 0.5283968528058267 1103 58-59 0.4890625959962591 1103 60-61 0.4975120206397179 1103 62-63 0.44962130432445235 1103 64-65 0.40604612793860895 1103 66-67 0.4746217750222783 1103 68-69 0.47967421020251777 1103 70-71 0.47739030657565706 1103 72-73 0.40715987397559417 1103 74-75 0.3698629344854183 1103 76-77 0.3433799810636913 1103 78-79 0.36607514676580877 1103 80-81 0.35604874548904775 1103 82-83 0.40068165051110327 1103 84-85 0.4105324906425736 1103 86-87 0.3792133157683111 1103 88-89 0.4102949107362406 1103 90-91 0.3738692621293964 1103 92-93 0.30952289706725367 1103 94-95 0.2861298502270131 1103 96-97 0.30930565944652244 1103 98-99 0.3499965299727634 1103 100 0.5444114777297067 1104 1 0.2026155751538461 1104 2 0.2724087498934118 1104 3 0.2276732816736846 1104 4 0.3589380792764274 1104 5 0.27491070956129704 1104 6 0.2452351369509458 1104 7 0.21949832164758476 1104 8 0.2484703546737066 1104 9 0.35074603675722216 1104 10-11 0.4701838706140862 1104 12-13 0.3866477255562941 1104 14-15 0.45801144155076656 1104 16-17 0.45366889746636474 1104 18-19 0.43729132283220196 1104 20-21 0.40312254868356945 1104 22-23 0.4011537680592525 1104 24-25 0.45220233867516413 1104 26-27 0.3955075939091586 1104 28-29 0.3804150872757788 1104 30-31 0.33150473573007133 1104 32-33 0.3945984303443524 1104 34-35 0.43145387081391107 1104 36-37 0.3925846262259185 1104 38-39 0.40293710478320577 1104 40-41 0.4403349441695994 1104 42-43 0.43409350679473846 1104 44-45 0.40942199829341064 1104 46-47 0.374913330921018 1104 48-49 0.33889860942624495 1104 50-51 0.28499286510645305 1104 52-53 0.30329904131732377 1104 54-55 0.3655664583356497 1104 56-57 0.4025855916081298 1104 58-59 0.39689349215223046 1104 60-61 0.3748492408409376 1104 62-63 0.43557385412366045 1104 64-65 0.39473537435748796 1104 66-67 0.39397358815695327 1104 68-69 0.39315573457397335 1104 70-71 0.4041006972545418 1104 72-73 0.3267941155562184 1104 74-75 0.3410953079915018 1104 76-77 0.3349682495010029 1104 78-79 0.37889949679765067 1104 80-81 0.38623234152358066 1104 82-83 0.394755083343135 1104 84-85 0.37941055991556993 1104 86-87 0.3652247926068597 1104 88-89 0.3451271599041732 1104 90-91 0.3282408206656555 1104 92-93 0.3511663492306667 1104 94-95 0.375920263432441 1104 96-97 0.35299973058026524 1104 98-99 0.34423582423235644 1104 100 0.38985728127369157 1105 1 0.19489229929254392 1105 2 0.2395749329637198 1105 3 0.21608947806620904 1105 4 0.28161833770961664 1105 5 0.22253237245777768 1105 6 0.19647530737758245 1105 7 0.24412998464175928 1105 8 0.24782672522308502 1105 9 0.32658367649614206 1105 10-11 0.3424386758255551 1105 12-13 0.26793470025030786 1105 14-15 0.4236099886301332 1105 16-17 0.4000449472568093 1105 18-19 0.3481029598302996 1105 20-21 0.36046254445598436 1105 22-23 0.36828532725117924 1105 24-25 0.3463498568799679 1105 26-27 0.39778200109284967 1105 28-29 0.45360596950829546 1105 30-31 0.4671921625134061 1105 32-33 0.4204389315837602 1105 34-35 0.44741307797344376 1105 36-37 0.4371867455458798 1105 38-39 0.4437291264868648 1105 40-41 0.4416622946077595 1105 42-43 0.43552281379982816 1105 44-45 0.45992631624491764 1105 46-47 0.48265536057360237 1105 48-49 0.3188133877801391 1105 50-51 0.36117264149753936 1105 52-53 0.4487996523628581 1105 54-55 0.431937532834354 1105 56-57 0.4159934976980679 1105 58-59 0.4790610623980456 1105 60-61 0.4426665804126202 1105 62-63 0.3622470298019138 1105 64-65 0.37834058631835177 1105 66-67 0.3888574972763621 1105 68-69 0.3716199181354227 1105 70-71 0.3504595669402022 1105 72-73 0.3513990647364196 1105 74-75 0.3153285646332975 1105 76-77 0.3015994485490836 1105 78-79 0.35096975326802493 1105 80-81 0.3039315907724287 1105 82-83 0.3219115084469806 1105 84-85 0.23755696038664809 1105 86-87 0.268516414644111 1105 88-89 0.23895819226915904 1105 90-91 0.29236129588677784 1105 92-93 0.3006575766639443 1105 94-95 0.33445830090605 1105 96-97 0.27058524142942986 1105 98-99 0.27163889069510105 1105 100 0.26769971434510964 1106 1 0.10715616480229428 1106 2 0.17864380036736094 1106 3 0.16822499637888555 1106 4 0.27537370781321613 1106 5 0.147534115452558 1106 6 0.22346672435359238 1106 7 0.21108875116757986 1106 8 0.24480647046718929 1106 9 0.3400035247845494 1106 10-11 0.38259087368965794 1106 12-13 0.2660436472821033 1106 14-15 0.4122883976663516 1106 16-17 0.27913967706838605 1106 18-19 0.35903011905940474 1106 20-21 0.39361997758253864 1106 22-23 0.37376539372399264 1106 24-25 0.43392727696351585 1106 26-27 0.38657565980797415 1106 28-29 0.4121078106945433 1106 30-31 0.4820289857096327 1106 32-33 0.3626748590624018 1106 34-35 0.35775776303989204 1106 36-37 0.38603578248206105 1106 38-39 0.3782825910483396 1106 40-41 0.42811810993777044 1106 42-43 0.32046482301407764 1106 44-45 0.41017376918708237 1106 46-47 0.3888375097517027 1106 48-49 0.31312391411389484 1106 50-51 0.37310879645215067 1106 52-53 0.3734755957636011 1106 54-55 0.38359879410613473 1106 56-57 0.36372375501916565 1106 58-59 0.39105271232406835 1106 60-61 0.3773763087246209 1106 62-63 0.4460484705894885 1106 64-65 0.40102955972030685 1106 66-67 0.3488907645209096 1106 68-69 0.41374017188903167 1106 70-71 0.33891029900351555 1106 72-73 0.27456653288697197 1106 74-75 0.28088813039244087 1106 76-77 0.27150965870965393 1106 78-79 0.26562525268917625 1106 80-81 0.23858473370868438 1106 82-83 0.29926112599592614 1106 84-85 0.3049338694340378 1106 86-87 0.28616324557119555 1106 88-89 0.31062686113109805 1106 90-91 0.25179182562172286 1106 92-93 0.2710333288914768 1106 94-95 0.2599540989393816 1106 96-97 0.26708757682216344 1106 98-99 0.3037967039211793 1106 100 0.3101131932404435 1107 1 0.0813293640401902 1107 2 0.1153875519011045 1107 3 0.13843868333997023 1107 4 0.2503127002858818 1107 5 0.12768689494181729 1107 6 0.2241573706384301 1107 7 0.20314376484358831 1107 8 0.14599481389559799 1107 9 0.2383844160789863 1107 10-11 0.36386993586415173 1107 12-13 0.2496041066105903 1107 14-15 0.3634908805173609 1107 16-17 0.24149302992834265 1107 18-19 0.2403558913562449 1107 20-21 0.24852274996258217 1107 22-23 0.33239333785721215 1107 24-25 0.3640934059635015 1107 26-27 0.32836336730466087 1107 28-29 0.30589997224100074 1107 30-31 0.40808686202628763 1107 32-33 0.3582346854081706 1107 34-35 0.2862605219387788 1107 36-37 0.3700355644663773 1107 38-39 0.37496656731727995 1107 40-41 0.3622131289641146 1107 42-43 0.4069103301532948 1107 44-45 0.40813964078111553 1107 46-47 0.3977733878651435 1107 48-49 0.3968114702634651 1107 50-51 0.388170493835851 1107 52-53 0.3916035204123318 1107 54-55 0.347164556376363 1107 56-57 0.34755496980885425 1107 58-59 0.2944441463090257 1107 60-61 0.3243100551292031 1107 62-63 0.3631269446482577 1107 64-65 0.3469241243893393 1107 66-67 0.28966535737399113 1107 68-69 0.27179284953231075 1107 70-71 0.26261436957740614 1107 72-73 0.2627712264380264 1107 74-75 0.24618540582839188 1107 76-77 0.2512889678632604 1107 78-79 0.3119745432956478 1107 80-81 0.21308079626128773 1107 82-83 0.19083229231661392 1107 84-85 0.2629897593856185 1107 86-87 0.22871295472564412 1107 88-89 0.30828880096509437 1107 90-91 0.25093901042844635 1107 92-93 0.26605040678627034 1107 94-95 0.2668010024321603 1107 96-97 0.2755560377473216 1107 98-99 0.26490370749053227 1107 100 0.2949515193434493 1108 1 0.028736225044294628 1108 2 0.07709879597035396 1108 3 0.06398121628353692 1108 4 0.13824538339928694 1108 5 0.10079884387131699 1108 6 0.1256567923616032 1108 7 0.11539276082752536 1108 8 0.13800821898989568 1108 9 0.14548037360523836 1108 10-11 0.25800611778002036 1108 12-13 0.13705823150017693 1108 14-15 0.28092387781266837 1108 16-17 0.23441414750522682 1108 18-19 0.21518081463211303 1108 20-21 0.19181132375890542 1108 22-23 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-0.750849194258187 2215 30-31 -3.3783099901171667 2215 32-33 -1.301023191533318 2215 34-35 -1.1794379722264026 2215 36-37 -0.9204048861430323 2215 38-39 -0.7269679802636091 2215 40-41 -0.8117932463017823 2215 42-43 -0.8355169036987959 2215 44-45 -0.8153116807351211 2215 46-47 -0.8971482383981808 2215 48-49 -0.9112864007790478 2215 50-51 -0.8585784358698518 2215 52-53 -0.8170131553457338 2215 54-55 -0.7921931590793321 2215 56-57 -0.8320292662229747 2215 58-59 -0.8971177791140335 2215 60-61 -0.7563246728809645 2215 62-63 -0.8015757163461643 2215 64-65 -0.8193803463943965 2215 66-67 -0.7583201128303898 2215 68-69 -0.707761370164441 2215 70-71 -0.8482315205587625 2215 72-73 -0.7995894365444727 2215 74-75 -0.8678694348071403 2215 76-77 -0.8270872839829337 2215 78-79 -0.7872819067503656 2215 80-81 -0.8014689120901224 2215 82-83 -0.7063474052306233 2215 84-85 -0.7142514787032326 2215 86-87 -0.7306793239908416 2215 88-89 -0.7163050307240582 2215 90-91 -0.7542185603010267 2215 92-93 -0.7222247452830004 2215 94-95 -0.7110217599736721 2215 96-97 -0.9801052957901675 2215 98-99 -0.8349582529485176 2215 100 -0.3507551938868758 2216 1 -0.4693897595985774 2216 2 -0.5914877324433903 2216 3 -0.5839975365206378 2216 4 -0.42481758476225195 2216 5 -0.4075786342795382 2216 6 -0.3583742430826504 2216 7 -0.441769737196509 2216 8 -0.523646991158607 2216 9 -0.6777474304370443 2216 10-11 -0.9221093864134389 2216 12-13 -0.5886397256809772 2216 14-15 -0.6586873335377348 2216 16-17 -0.7525361060257509 2216 18-19 -0.9834877032923472 2216 20-21 -0.9986342764297476 2216 22-23 -0.8454550897780564 2216 24-25 -0.892027028617008 2216 26-27 -1.3040539980049886 2216 28-29 -1.1512056493801097 2216 30-31 -1.2609061718562415 2216 32-33 -1.5300167331440022 2216 34-35 -1.1292858077812014 2216 36-37 -1.229773443567197 2216 38-39 -1.0839940689679821 2216 40-41 -1.0161755504048244 2216 42-43 -1.046087802683921 2216 44-45 -0.8933304497130905 2216 46-47 -1.0623118834996674 2216 48-49 -1.099097156145298 2216 50-51 -1.0890777214290068 2216 52-53 -1.0927801677358033 2216 54-55 -1.1133876852402054 2216 56-57 -1.0612642769832092 2216 58-59 -1.0114177269631668 2216 60-61 -1.0426073004170604 2216 62-63 -1.1496573353179755 2216 64-65 -1.1014168861050635 2216 66-67 -1.1081513763008957 2216 68-69 -1.0477974789280822 2216 70-71 -0.9406721533857336 2216 72-73 -0.8848853877600007 2216 74-75 -0.9526650905217835 2216 76-77 -0.9195810595168581 2216 78-79 -0.8074338215217836 2216 80-81 -0.8998121770882719 2216 82-83 -0.9074603108772941 2216 84-85 -0.8952328044427702 2216 86-87 -0.8471667933925495 2216 88-89 -0.9630482471670376 2216 90-91 -1.0150584380741954 2216 92-93 -0.9641041374623924 2216 94-95 -0.9635410043761716 2216 96-97 -1.0020256475382254 2216 98-99 -1.0302263284230087 2216 100 -0.7019059956444629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 2.0 2 13.0 3 29.0 4 54.0 5 32.0 6 61.0 7 225.0 8 739.0 9 1139.0 10 1605.0 11 2777.0 12 4724.0 13 6915.0 14 9766.0 15 12764.0 16 15835.0 17 18733.0 18 21791.0 19 24755.0 20 28300.0 21 32841.0 22 38109.0 23 43612.0 24 50838.0 25 58482.0 26 66107.0 27 72707.0 28 82766.0 29 101532.0 30 119220.0 31 138313.0 32 170587.0 33 221682.0 34 325856.0 35 466994.0 36 745237.0 37 1287816.0 38 1093353.0 39 424360.0 40 898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70307207796066 21.076783138151566 12.2049990489086 24.015145734979175 2 15.586384014152541 19.840298963264615 40.65887396079586 23.914443061786983 3 18.359785589304344 26.933525023308185 29.08754213132216 25.61914725606531 4 12.032032753182628 16.586007854438108 40.45761326995897 30.924346122420292 5 12.858977715383116 38.87570128106238 35.28139973328039 12.983921270274118 6 29.58161854843938 38.941264880039874 18.11742315160347 13.359693419917267 7 26.63137439559204 32.0626827279883 23.941758967727424 17.36418390869223 8 26.20504599503398 35.14185404017652 19.999546691161743 18.653553273627757 9 26.287359435962255 14.925712470486868 21.62815859485334 37.15876949869754 10-11 25.65435289119516 25.88805945105533 27.660233901586196 20.797353756163314 12-13 25.674772012976426 23.96983283294258 27.65399490332338 22.701400250757615 14-15 23.09819615265208 25.173384014251283 24.9979575009088 26.730462332187837 16-17 21.993912542276576 27.686160780604403 26.367808985215508 23.952117691903517 18-19 21.372809988289482 28.506535663489103 28.010090547864213 22.110563800357202 20-21 23.208746291295835 27.593962550708206 26.890932888717835 22.306358269278125 22-23 23.78098458771582 27.25119515965829 26.50148932644985 22.466330926176038 24-25 23.126199706755433 27.271859151242335 27.430675068677036 22.1712660733252 26-27 23.021712490108904 27.570945093835732 26.32680386948241 23.080538546572956 28-29 22.053151468674724 28.13289000469749 26.927611141034753 22.886347385593037 30-31 22.904718923362893 27.717092315134195 26.942643979549757 22.435544781953155 32-33 23.39819372758775 27.914955369438577 25.883494120376753 22.80335678259692 34-35 23.29969795542503 27.63327021739988 27.032844503313214 22.03418732386187 36-37 22.861781660823503 27.27050770739867 26.68967325865855 23.178037373119274 38-39 23.099485488353817 27.309161371670264 27.255763349242045 22.33558979073387 40-41 22.66491449925561 26.390387537893783 27.997217476622392 22.947480486228216 42-43 22.627524435705585 26.74673330285725 27.215261330060446 23.410480931376714 44-45 22.762779491658414 27.796427884188724 27.06088166784261 22.379910956310248 46-47 22.805968869312213 27.417686682247442 26.594612813487462 23.18173163495288 48-49 23.51951322788701 27.007766699731224 26.829580115294515 22.643139957087254 50-51 23.054506794128887 26.83623359678064 26.799207461460394 23.310052147630074 52-53 22.406540450589603 27.5154041456268 26.61703328307724 23.46102212070636 54-55 22.445436656121682 27.43398607724888 26.608546250954657 23.51203101567478 56-57 23.683259533467464 26.767311074495996 26.316542776553124 23.232886615483412 58-59 22.475133232243124 27.23925676379773 27.3738377159494 22.91177228800974 60-61 22.532819417553526 27.941965169049922 26.52649434532146 22.9987210680751 62-63 23.01367299468236 27.148017580505936 26.1060321518548 23.732277272956903 64-65 22.06537234695884 27.428835054276345 26.633329051695764 23.87246354706905 66-67 22.926885242041525 26.926246669856603 26.56454139042504 23.58232669767684 68-69 22.250494461582228 27.007446376626465 26.895960257878198 23.84609890391311 70-71 22.287108192711596 26.70011658564828 27.03687954602761 23.975895675612513 72-73 22.927653661543847 26.80103252383303 27.47528664695696 22.796027167666168 74-75 23.14110398185187 27.100174048422538 26.947817981020727 22.810903988704865 76-77 22.790305826644612 27.04467442413972 26.626882110312877 23.538137638902786 78-79 22.596995522857778 27.01136216072412 26.73730215587779 23.654340160540315 80-81 22.20798827906385 27.386477591950676 27.387593386274112 23.01794074271136 82-83 23.09802643369963 26.25245553677419 28.50827239150832 22.14124563801786 84-85 23.31924937860125 26.91881546475689 26.98025467091279 22.78168048572907 86-87 21.894325818797522 27.648149492036545 27.497351547400932 22.960173141765004 88-89 23.151391088336908 26.268202094923904 27.206959944299356 23.373446872439825 90-91 22.72323913764697 26.354947452471055 27.466363536682053 23.455449873199914 92-93 23.08875194206812 26.529520314006188 27.40193544374038 22.979792300185306 94-95 21.81434605457977 26.606924458211566 28.099120840522463 23.4796086466862 96-97 22.28733406875553 26.56963673915343 27.539104300743865 23.60392489134717 98-99 21.59313416483961 27.381264795638437 27.344391286184383 23.68120975333757 100 22.471368793195623 26.609570128951628 26.402275171373287 24.516785906479466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1421.0 1 948.0 2 577.0 3 734.0 4 750.0 5 660.0 6 587.0 7 571.5 8 620.5 9 650.5 10 570.0 11 490.5 12 511.0 13 542.0 14 572.0 15 928.5 16 1148.0 17 1327.5 18 1787.0 19 2148.5 20 2637.5 21 3356.5 22 4638.5 23 6320.5 24 8080.5 25 10168.5 26 13460.0 27 17297.5 28 22740.0 29 34711.0 30 51499.5 31 67140.0 32 87378.0 33 106285.5 34 118813.5 35 133853.5 36 148024.5 37 175420.5 38 186154.0 39 170875.5 40 157100.5 41 143325.5 42 141095.0 43 151824.0 44 168388.0 45 203217.0 46 237663.0 47 259843.5 48 272797.5 49 263901.5 50 250092.0 51 259351.5 52 263697.0 53 249992.0 54 236562.0 55 204298.5 56 177519.0 57 144921.5 58 112759.0 59 109028.5 60 89884.0 61 60633.0 62 44995.0 63 37704.0 64 27723.0 65 13039.0 66 6697.5 67 4716.0 68 3449.5 69 2431.5 70 1821.0 71 1387.5 72 997.5 73 797.0 74 608.5 75 463.0 76 320.0 77 204.0 78 126.0 79 80.0 80 61.0 81 49.0 82 39.5 83 35.5 84 28.5 85 24.0 86 21.5 87 18.5 88 17.5 89 14.0 90 14.0 91 14.5 92 12.0 93 9.5 94 10.0 95 10.0 96 7.5 97 4.5 98 5.5 99 5.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05915767690772087 2 0.007642883710976709 3 0.007642883710976709 4 0.00911875091033773 5 0.0034963996746767015 6 0.0023894992751559365 7 8.609225329605948E-4 8 0.0014934370469724604 9 6.149446664004249E-4 10-11 5.270954283432213E-4 12-13 2.9868740939449206E-4 14-15 2.1083817133728854E-4 16-17 5.358803521489417E-4 18-19 0.0018272641515898339 20-21 0.0010454059328807222 22-23 0.0012298893328008499 24-25 0.0013001687232466125 26-27 0.0037511624650425923 28-29 0.004049849874437084 30-31 0.005130395502540688 32-33 0.006070382349752766 34-35 0.003347055969979456 36-37 0.007844936958508276 38-39 0.006096737121169927 40-41 0.005833189406998316 42-43 0.013484858041780744 44-45 0.019397111763030547 46-47 0.017754331011360838 48-49 0.020829054343362963 50-51 0.01746442852577207 52-53 0.024246389703788184 54-55 0.01804423349694961 56-57 0.014767456917415918 58-59 0.016550796449977152 60-61 0.01551417544090215 62-63 0.010656112576338791 64-65 0.010541908566864428 66-67 0.013142246013357652 68-69 0.004875632712174798 70-71 0.008328107767822899 72-73 0.00817876406312565 74-75 0.010963584909539005 76-77 0.010252006081275656 78-79 0.011631239118773751 80-81 0.009768835271961035 82-83 0.006131876816392808 84-85 0.010146586995607011 86-87 0.008960622281834764 88-89 0.007581389244336667 90-91 0.012667860127848752 92-93 0.008275398224988576 94-95 0.006931304882713361 96-97 0.008907912739000441 98-99 0.008635580101023109 100 0.25711714994582335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5691569.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.480916183431205 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68611532754507 46.14181194807102 2 15.533550056144255 19.721679782904527 3 5.664349806928659 10.787343459818183 4 2.563670605041569 6.509766352022808 5 1.3368637675299613 4.243266838761776 6 0.7335589226864205 2.7940195491998847 7 0.4521281046212382 2.0091054419544108 8 0.295696307718282 1.501685802081147 9 0.1990553117418704 1.1372592204497198 >10 0.5304055910485947 4.7863015898991845 >50 0.0038390174833743845 0.15926262016077783 >100 5.166635880585794E-4 0.04509297121397133 >500 1.6701195237511988E-4 0.06674004124781921 >1k 8.350597016707474E-5 0.09666438221471237 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7569847611440712E-5 0.0 5 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 6 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 7 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 8 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 9 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 10-11 0.0 0.0 0.0 1.7569847611440712E-5 5.2709542834322136E-5 12-13 0.0 0.0 0.0 1.7569847611440712E-5 1.2298893328008498E-4 14-15 0.0 0.0 0.0 1.7569847611440712E-5 1.5812862850296641E-4 16-17 0.0 0.0 0.0 1.7569847611440712E-5 2.1083817133728854E-4 18-19 0.0 0.0 0.0 2.6354771417161068E-5 2.547627903658903E-4 20-21 0.0 0.0 0.0 5.2709542834322136E-5 3.1625725700593283E-4 22-23 0.0 0.0 0.0 1.4934370469724606E-4 3.3382710461737355E-4 24-25 0.0 0.0 0.0 2.547627903658903E-4 3.3382710461737355E-4 26-27 0.0 0.0 0.0 6.412994378175859E-4 3.6018187603453457E-4 28-29 0.0 0.0 0.0 0.002846315313053395 3.777517236459753E-4 30-31 0.0 0.0 0.0 0.010269575928887095 3.95321571257416E-4 32-33 0.0 0.0 0.0 0.025326935331891787 4.3046126648029745E-4 34-35 0.0 0.0 0.0 0.04851034925518781 4.6560096170317883E-4 36-37 0.0 0.0 0.0 0.08899127815194721 4.743858855088992E-4 38-39 0.0 0.0 0.0 0.14770970886938206 4.8317080931461955E-4 40-41 0.0 0.0 0.0 0.21031107590894532 4.919557331203399E-4 42-43 0.0 0.0 0.0 0.27398420365280646 4.919557331203399E-4 44-45 0.0 0.0 0.0 0.3532769259232384 5.095255807317806E-4 46-47 0.0 0.0 0.0 0.4502185601193625 5.270954283432213E-4 48-49 0.0 0.0 0.0 0.5655206850694421 5.270954283432213E-4 50-51 0.0 0.0 0.0 0.6881494364734926 5.446652759546621E-4 52-53 0.0 0.0 0.0 0.8078352384026267 6.149446664004249E-4 54-55 0.0 0.0 0.0 0.9406720712689243 6.149446664004249E-4 56-57 0.0 0.0 0.0 1.1011901990470465 6.41299437817586E-4 58-59 0.0 0.0 0.0 1.2985698671139716 6.500843616233064E-4 60-61 0.0 0.0 0.0 1.5032058822444214 6.500843616233064E-4 62-63 0.0 0.0 0.0 1.7175931627992211 7.027939044576284E-4 64-65 0.0 0.0 0.0 1.9427595448636394 7.203637520690691E-4 66-67 0.0 0.0 0.0 2.2035312231126425 7.379335996805099E-4 68-69 0.0 0.0 0.0 2.492186249521002 7.555034472919506E-4 70-71 0.0 0.0 0.0 2.8139867934483442 7.555034472919506E-4 72-73 0.0 0.0 0.0 3.1500979782552054 7.730732949033913E-4 74-75 0.0 0.0 0.0 3.4783115165607237 7.994280663205524E-4 76-77 0.0 0.0 0.0 3.860192154395387 8.082129901262727E-4 78-79 0.0 0.0 0.0 4.271994242712335 8.609225329605949E-4 80-81 0.0 0.0 0.0 4.723091295212269 8.609225329605949E-4 82-83 0.0 0.0 0.0 5.198970617768141 8.960622281834763E-4 84-85 0.0 0.0 0.0 5.665177739213915 8.960622281834763E-4 86-87 0.0 0.0 0.0 6.163968143055104 9.224169996006374E-4 88 0.0 0.0 0.0 6.5578753415798 9.487717710177984E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5915 0.0 41.731644 94 GTACTAG 6655 0.0 34.622417 1 TAGGCAT 8005 0.0 31.935665 5 CTGTGCG 8325 0.0 31.553179 9 TAGTACT 8725 0.0 31.13265 4 GCATCGT 6825 0.0 31.05226 8 GGTATCA 8315 0.0 30.933887 1 TACCCCG 385 0.0 30.515305 5 GTATCAA 12295 0.0 30.443457 1 CAACGCA 11110 0.0 30.031893 5 ACTGTGC 9450 0.0 29.686697 8 GTACTGT 9375 0.0 29.122458 6 TATTCTC 3220 0.0 29.042608 5 CTAGGCA 9115 0.0 29.027218 4 ATCAACG 11685 0.0 28.836605 3 TACTGTG 10195 0.0 28.669909 7 CAGTACG 790 0.0 27.956724 9 CTAGTAC 10190 0.0 27.901972 3 CGCCTAC 3140 0.0 26.938042 7 AGGGCTA 7090 0.0 26.51259 5 >>END_MODULE