FastQCFastQC Report
Mon 16 Jan 2017
ERR1146671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691569
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169700.2981603139661489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145840.2562386575652513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97130.17065592984992362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126750.046.5767441
GGGCACC60100.042.56762794
GTGGTAT26400.042.0514951
GTATCAA218500.039.311781
GTACTAG61350.032.7406041
CTGTGCG84850.032.1864559
ATCAACG262200.031.752623
CAACGCA262400.031.6856985
TAGTACT89250.030.7782294
TATCAAC286000.030.01722
ACTGTGC94750.029.910138
TCTAACG31150.029.5986142
CTAACGC30850.029.5791423
CGCCTAC30550.029.5367417
TAACGCC31450.028.865274
GTACTGT95950.028.8498066
TAGGCAT79550.028.7141115
AACGCAG304100.028.3899966
TACTGTG105500.028.3319997
TGAACCG7850.028.1402195
Produced by FastQC (version 0.11.5)