FastQCFastQC Report
Mon 16 Jan 2017
ERR1146670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6211838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC67400.040.96720594
GTACTAG66650.036.5414961
CAACGCA112950.033.617745
GTATCAA131100.032.4207041
GCATCGT67850.032.3434228
TAGGCAT80000.031.9559945
CTGTGCG91850.031.9245179
ATCAACG122400.031.139443
TAGTACT97750.030.3856264
GGTATCA86250.029.8184411
CTAGGCA90750.029.20794
ACTGTGC106900.029.0124998
GTACTGT107650.028.3743086
TACTGTG115350.027.9056367
CTAGTAC111300.027.8266943
CTAACGC35350.027.7861423
TAACGCC35400.027.6139144
TCTAACG35900.027.491362
AACGCAG145700.027.4793196
CGCCTAC36150.026.9079727
Produced by FastQC (version 0.11.5)