##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146670_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6211838
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482753896672772	31.0	31.0	34.0	28.0	34.0
2	31.593996173113336	33.0	31.0	34.0	28.0	34.0
3	31.72967968578704	33.0	31.0	34.0	28.0	34.0
4	35.14953931509482	37.0	35.0	37.0	32.0	37.0
5	35.330052232527635	37.0	35.0	37.0	32.0	37.0
6	35.387079637298974	37.0	35.0	37.0	32.0	37.0
7	35.301293755568	37.0	35.0	37.0	32.0	37.0
8	35.312995123182546	37.0	35.0	37.0	32.0	37.0
9	36.84284120094568	39.0	37.0	39.0	32.0	39.0
10-11	36.68086088207709	39.0	37.0	39.0	32.0	39.0
12-13	36.62591039882238	39.0	37.0	39.0	32.0	39.0
14-15	37.841601793221265	40.0	38.0	41.0	32.0	41.0
16-17	37.87377906506899	40.0	38.0	41.0	32.0	41.0
18-19	37.84602544367706	40.0	38.0	41.0	32.0	41.0
20-21	37.90699588430993	40.0	38.0	41.0	32.0	41.0
22-23	37.854921280947764	40.0	38.0	41.0	32.0	41.0
24-25	37.82316103542945	40.0	37.5	41.0	32.0	41.0
26-27	37.85723074233424	40.0	37.0	41.0	32.0	41.0
28-29	37.844104595129494	40.0	37.0	41.0	32.0	41.0
30-31	37.71568632343599	40.0	37.0	41.0	32.0	41.0
32-33	37.70621336229309	40.0	37.0	41.0	31.5	41.0
34-35	37.65359664885015	40.0	37.0	41.0	31.0	41.0
36-37	37.54656117883306	40.0	37.0	41.0	31.0	41.0
38-39	37.44300889688366	40.0	37.0	41.0	31.0	41.0
40-41	37.35482517412721	40.0	36.0	41.0	31.0	41.0
42-43	37.1991516842519	40.0	36.0	41.0	30.0	41.0
44-45	37.050833102859414	40.0	36.0	41.0	30.0	41.0
46-47	37.04291580044425	40.0	35.5	41.0	30.0	41.0
48-49	36.908468314853025	39.0	35.0	41.0	30.0	41.0
50-51	36.733073125860656	39.0	35.0	41.0	30.0	41.0
52-53	36.5251700060433	39.0	35.0	41.0	30.0	41.0
54-55	36.30646927688713	38.5	35.0	40.5	29.0	41.0
56-57	36.074859164067064	38.0	35.0	40.0	29.0	41.0
58-59	35.78199350981143	37.5	34.0	40.0	28.5	41.0
60-61	35.53400603814845	37.0	34.0	40.0	28.0	41.0
62-63	35.23287326552946	36.5	34.0	40.0	28.0	41.0
64-65	35.0782282152239	36.0	34.0	40.0	28.0	41.0
66-67	34.82155949656124	35.5	34.0	39.0	28.0	41.0
68-69	34.691625409419885	35.0	34.0	39.0	28.0	41.0
70-71	34.59620598605437	35.0	34.0	39.0	28.0	41.0
72-73	34.36931267364024	35.0	34.0	38.0	28.0	40.5
74-75	34.10756252819213	35.0	34.0	37.0	28.0	40.0
76-77	33.836438989555106	35.0	33.0	37.0	27.5	39.0
78-79	33.57770630850322	35.0	33.0	36.0	27.0	39.0
80-81	33.38136538654099	35.0	33.0	36.0	27.0	39.0
82-83	33.11842082810273	35.0	33.0	36.0	27.0	37.0
84-85	32.934078689753335	35.0	33.0	35.0	26.5	37.0
86-87	32.756820284109146	35.0	33.0	35.0	26.0	36.5
88-89	32.623624440946465	35.0	33.0	35.0	26.0	36.0
90-91	32.47062141672078	35.0	33.0	35.0	26.0	36.0
92-93	32.32107340854671	35.0	33.0	35.0	25.0	36.0
94-95	32.25027359052184	35.0	33.0	35.0	25.0	36.0
96-97	32.14286657185844	35.0	33.0	35.0	25.0	35.0
98-99	32.050468959428756	35.0	32.0	35.0	25.0	35.0
100	31.124649580365748	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33102182561518134
1101	2	0.44006345725411933
1101	3	0.4770456538837067
1101	4	0.5505563101172086
1101	5	0.4232962782792882
1101	6	0.44151415469760735
1101	7	0.45594937672598945
1101	8	0.4574940875098008
1101	9	0.6537582448409296
1101	10-11	0.7008936197714846
1101	12-13	0.5987178073074162
1101	14-15	0.8057805111490595
1101	16-17	0.779921818165235
1101	18-19	0.7752061337490161
1101	20-21	0.7410354539603645
1101	22-23	0.750338087655841
1101	24-25	0.7977907260592545
1101	26-27	0.6877509866674743
1101	28-29	0.6774699909923214
1101	30-31	0.7527276946670796
1101	32-33	0.7446022463271973
1101	34-35	0.7727846712551951
1101	36-37	0.7456506920923189
1101	38-39	0.7195540023104314
1101	40-41	0.7561768911248663
1101	42-43	0.6987552914866413
1101	44-45	0.7452147189534699
1101	46-47	0.7383103184885726
1101	48-49	0.7083059299922283
1101	50-51	0.6727808058336109
1101	52-53	0.6189616855204036
1101	54-55	0.6565505432794012
1101	56-57	0.5701891319544217
1101	58-59	0.5686336664313814
1101	60-61	0.5334142575297989
1101	62-63	0.5968428485932549
1101	64-65	0.5690439248669747
1101	66-67	0.5546269599502409
1101	68-69	0.6079260079658724
1101	70-71	0.6217305148682897
1101	72-73	0.6046737997076477
1101	74-75	0.5505909203154928
1101	76-77	0.5449834917187957
1101	78-79	0.499648213556803
1101	80-81	0.5038810072874398
1101	82-83	0.5387145935790372
1101	84-85	0.5123647790288501
1101	86-87	0.518597585618302
1101	88-89	0.5895522603957417
1101	90-91	0.5573999764815909
1101	92-93	0.5818385832826252
1101	94-95	0.5653064926914979
1101	96-97	0.561048481608232
1101	98-99	0.5347307652986402
1101	100	0.6046702938101873
1102	1	0.3091747544384411
1102	2	0.399035783362919
1102	3	0.4264033993913259
1102	4	0.48432682071824473
1102	5	0.4184410250569002
1102	6	0.38222499537407373
1102	7	0.3726039136967856
1102	8	0.38272024975899654
1102	9	0.4960224886177258
1102	10-11	0.6312061744394839
1102	12-13	0.5519178473929855
1102	14-15	0.7135314146029472
1102	16-17	0.6942336165437126
1102	18-19	0.7065870942637034
1102	20-21	0.6032248176107089
1102	22-23	0.6508753160548792
1102	24-25	0.6782001237426343
1102	26-27	0.6148512365590619
1102	28-29	0.6600810098700975
1102	30-31	0.6917626520932387
1102	32-33	0.6796588818822897
1102	34-35	0.7422904570208715
1102	36-37	0.743920949815994
1102	38-39	0.716523126814792
1102	40-41	0.638345511605614
1102	42-43	0.6911339911530661
1102	44-45	0.6220392852761449
1102	46-47	0.621461146551745
1102	48-49	0.6357108119347501
1102	50-51	0.6148368913238613
1102	52-53	0.5536551250264949
1102	54-55	0.5428232691877355
1102	56-57	0.5779235104347578
1102	58-59	0.565961818022167
1102	60-61	0.5644664973307414
1102	62-63	0.5576164246126254
1102	64-65	0.5299878129335838
1102	66-67	0.5528653747407404
1102	68-69	0.4859393440404034
1102	70-71	0.45315227960047366
1102	72-73	0.4561949469489761
1102	74-75	0.4049997689663485
1102	76-77	0.47695459866412193
1102	78-79	0.4214347434560821
1102	80-81	0.43317871735174407
1102	82-83	0.44981680693287984
1102	84-85	0.4750539933509259
1102	86-87	0.45568645146275344
1102	88-89	0.5171855022262903
1102	90-91	0.4441802636572092
1102	92-93	0.46116700867498395
1102	94-95	0.4665567526560963
1102	96-97	0.5342206714356266
1102	98-99	0.4007684585965592
1102	100	0.5735979437278687
1103	1	0.2314026402401801
1103	2	0.3072614916033167
1103	3	0.32251388597984487
1103	4	0.34223976459135486
1103	5	0.2306283196229586
1103	6	0.2708822079028721
1103	7	0.2496483753668457
1103	8	0.2784789811691155
1103	9	0.3648054951323658
1103	10-11	0.4774138228905258
1103	12-13	0.4175186072128696
1103	14-15	0.5432045966836156
1103	16-17	0.5092390714650463
1103	18-19	0.5213175739011504
1103	20-21	0.4548147520419832
1103	22-23	0.5324350325434821
1103	24-25	0.5030440003532277
1103	26-27	0.413317004384659
1103	28-29	0.4588811539441764
1103	30-31	0.45697118234080136
1103	32-33	0.4169117987239588
1103	34-35	0.45390328987382134
1103	36-37	0.43096105836198717
1103	38-39	0.512915062386611
1103	40-41	0.4387931500492641
1103	42-43	0.40259906146413016
1103	44-45	0.3745763592162703
1103	46-47	0.4033875155425193
1103	48-49	0.3814829084017646
1103	50-51	0.3641292428215763
1103	52-53	0.3244681199512556
1103	54-55	0.3641289289101124
1103	56-57	0.37712699557833673
1103	58-59	0.35742945013996064
1103	60-61	0.3306558193062372
1103	62-63	0.2993345285558675
1103	64-65	0.3341281056488299
1103	66-67	0.3876957174376017
1103	68-69	0.37435237395619225
1103	70-71	0.3508540368697197
1103	72-73	0.31860049473899466
1103	74-75	0.24556065525616333
1103	76-77	0.24116664938451038
1103	78-79	0.28310077389890154
1103	80-81	0.22417296740498927
1103	82-83	0.29021273751904175
1103	84-85	0.2738617411072042
1103	86-87	0.25030861238747093
1103	88-89	0.2919140027284115
1103	90-91	0.2887994215438283
1103	92-93	0.27042693684190766
1103	94-95	0.2492729949719532
1103	96-97	0.3001090426164694
1103	98-99	0.308318809962266
1103	100	0.40417451627952516
1104	1	0.27262607700145125
1104	2	0.32185138751835396
1104	3	0.2580690588090704
1104	4	0.3867078537842019
1104	5	0.24582280403756585
1104	6	0.23582662388265163
1104	7	0.28228569026542516
1104	8	0.36616919459072506
1104	9	0.372319297530602
1104	10-11	0.5485383393118752
1104	12-13	0.41846795253744773
1104	14-15	0.5109767813057502
1104	16-17	0.508165121156388
1104	18-19	0.48898172267372075
1104	20-21	0.5019260213523893
1104	22-23	0.48912897743361583
1104	24-25	0.5423370814124624
1104	26-27	0.47352288555075006
1104	28-29	0.4019979692888711
1104	30-31	0.4192040197487614
1104	32-33	0.45722686739451035
1104	34-35	0.4565472338812242
1104	36-37	0.45628010847490685
1104	38-39	0.4733043365292673
1104	40-41	0.4495311677943974
1104	42-43	0.4710908982219877
1104	44-45	0.47532905868173003
1104	46-47	0.4230160652537478
1104	48-49	0.3752725324593129
1104	50-51	0.3581486522197821
1104	52-53	0.4017569606700846
1104	54-55	0.4630896034243648
1104	56-57	0.4689604141439929
1104	58-59	0.4199888819824622
1104	60-61	0.42712489733629866
1104	62-63	0.46984032572802903
1104	64-65	0.47234839557194164
1104	66-67	0.4583325871945263
1104	68-69	0.44398280671651946
1104	70-71	0.39217870116074494
1104	72-73	0.4075971901757711
1104	74-75	0.37938648696486865
1104	76-77	0.38887255358855555
1104	78-79	0.4084239902427811
1104	80-81	0.3834821825452721
1104	82-83	0.41773745408438145
1104	84-85	0.44002027148209066
1104	86-87	0.3761536079341141
1104	88-89	0.3614864162361968
1104	90-91	0.4020267998797067
1104	92-93	0.4224626656917607
1104	94-95	0.4644384418722467
1104	96-97	0.45053104810521916
1104	98-99	0.4288309753081876
1104	100	0.4560243883049395
1105	1	0.15668576193485606
1105	2	0.19370549778140855
1105	3	0.25411623013460627
1105	4	0.31951915694890687
1105	5	0.19287733474558877
1105	6	0.21017783729571704
1105	7	0.24646340904330089
1105	8	0.21555069392706372
1105	9	0.3459419165328015
1105	10-11	0.4021956468931762
1105	12-13	0.3126273347415349
1105	14-15	0.4266362344637926
1105	16-17	0.375501950630607
1105	18-19	0.392604427376817
1105	20-21	0.37287930916487255
1105	22-23	0.4088842441528229
1105	24-25	0.4179941577381001
1105	26-27	0.39538942586329284
1105	28-29	0.40653938194695627
1105	30-31	0.421103195009799
1105	32-33	0.43253939532992547
1105	34-35	0.44762439117688757
1105	36-37	0.4405064873579505
1105	38-39	0.4230495849043763
1105	40-41	0.38689982717566807
1105	42-43	0.39180237387798655
1105	44-45	0.4068405629563614
1105	46-47	0.41405131800006956
1105	48-49	0.3570748123676637
1105	50-51	0.4090945155079737
1105	52-53	0.4179474619651984
1105	54-55	0.3292852262599837
1105	56-57	0.3650879084105725
1105	58-59	0.3292440561401335
1105	60-61	0.3574039155358406
1105	62-63	0.35096819083334907
1105	64-65	0.3957293745032402
1105	66-67	0.32185067799363054
1105	68-69	0.2891736174921675
1105	70-71	0.31890814647215393
1105	72-73	0.33807870524370287
1105	74-75	0.26868969374213947
1105	76-77	0.25189674166483655
1105	78-79	0.3119747267029851
1105	80-81	0.21509095620231022
1105	82-83	0.3072305875048684
1105	84-85	0.2808059818962789
1105	86-87	0.26263607555843294
1105	88-89	0.2944986948503683
1105	90-91	0.2752866841224062
1105	92-93	0.25609275548761445
1105	94-95	0.2149690808786815
1105	96-97	0.1894609419879032
1105	98-99	0.18925098539263274
1105	100	0.24572717667034993
1106	1	0.11251775961713051
1106	2	0.2477700619681542
1106	3	0.20657616797004152
1106	4	0.28433776504546415
1106	5	0.17642170028616277
1106	6	0.22239647696320475
1106	7	0.20009788853171528
1106	8	0.20116387266750024
1106	9	0.34798132715651064
1106	10-11	0.34770316746519825
1106	12-13	0.2770171919215514
1106	14-15	0.4225429098513942
1106	16-17	0.30961013970768647
1106	18-19	0.3821242232714823
1106	20-21	0.3629767161391442
1106	22-23	0.3709903216543182
1106	24-25	0.42115761763270143
1106	26-27	0.38235178590361585
1106	28-29	0.3943888883025508
1106	30-31	0.48433167572172664
1106	32-33	0.4382452599189435
1106	34-35	0.3909686507450232
1106	36-37	0.398901561702921
1106	38-39	0.4099138190697431
1106	40-41	0.38824005795312644
1106	42-43	0.382233418723267
1106	44-45	0.3804546042456636
1106	46-47	0.39167736499678085
1106	48-49	0.3361933754741031
1106	50-51	0.3836032681732888
1106	52-53	0.33749884132866725
1106	54-55	0.3816255228798582
1106	56-57	0.3575308800579222
1106	58-59	0.34368104540781985
1106	60-61	0.3660729418417148
1106	62-63	0.3974605415673338
1106	64-65	0.3037098010521504
1106	66-67	0.30620574754974683
1106	68-69	0.30687301720899995
1106	70-71	0.33185216330740275
1106	72-73	0.3091749722688846
1106	74-75	0.3279435762126681
1106	76-77	0.290168906204201
1106	78-79	0.2883265337826728
1106	80-81	0.30224114856377327
1106	82-83	0.29528944702006044
1106	84-85	0.2856836867518595
1106	86-87	0.34632322942116645
1106	88-89	0.34830912577601936
1106	90-91	0.31260479334278557
1106	92-93	0.3189754150621198
1106	94-95	0.31737866047989627
1106	96-97	0.3035817743926472
1106	98-99	0.3228031600990917
1106	100	0.33484698977112615
1107	1	0.11317899080657057
1107	2	0.16540442362179775
1107	3	0.11914497357066978
1107	4	0.2822122067355366
1107	5	0.17180786454129304
1107	6	0.21468127970112505
1107	7	0.18090231380330124
1107	8	0.1389325247913078
1107	9	0.1858716877431874
1107	10-11	0.31857341430853836
1107	12-13	0.2106632452649606
1107	14-15	0.3394491202037315
1107	16-17	0.23995081226141934
1107	18-19	0.17472266019584737
1107	20-21	0.2047905801215819
1107	22-23	0.2984235912922699
1107	24-25	0.33131986486802845
1107	26-27	0.18996995309762355
1107	28-29	0.23147928634134018
1107	30-31	0.35135323606181856
1107	32-33	0.28515224717561694
1107	34-35	0.3037331344866345
1107	36-37	0.2988093430702534
1107	38-39	0.34102741645914136
1107	40-41	0.26408457568112453
1107	42-43	0.30079542555932903
1107	44-45	0.31182549388018543
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2208	96-97	0.17110668274285956
2208	98-99	0.11339154223795589
2208	100	-0.08989807700001862
2209	1	0.07242902849711541
2209	2	0.10798426187749399
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2209	4	0.14293743159940675
2209	5	0.07367352921577464
2209	6	0.01453376789948635
2209	7	-0.00953599265333338
2209	8	0.02615428904075401
2209	9	0.06538513047361505
2209	10-11	0.1523677940604884
2209	12-13	0.03313433938343735
2209	14-15	0.1319091180875276
2209	16-17	0.05618342965881595
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2209	20-21	0.04420720483302176
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2211	2	-0.19771981677555672
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2211	4	0.021755100034376085
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2211	10-11	-0.13033430834663307
2211	12-13	-0.15775589339304474
2211	14-15	-0.22454104754270077
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2211	22-23	-0.10648759222807058
2211	24-25	-0.051280933026284004
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2211	28-29	-0.07239728297970771
2211	30-31	-0.07503505239930774
2211	32-33	-0.16085778131812845
2211	34-35	-0.20287917599877403
2211	36-37	-0.2276335904122817
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2211	40-41	-0.16341493827254538
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2211	44-45	-0.29081547963500753
2211	46-47	-0.1786218002340405
2211	48-49	-0.048564144281876054
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2211	66-67	-0.06741821747138488
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2211	74-75	4.3447672089769185E-4
2211	76-77	0.023970742802077893
2211	78-79	-0.02532791592285122
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2211	92-93	0.03171705552990289
2211	94-95	-0.017681766717750236
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2212	9	-0.32190359439927363
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2213	8	-0.2930433029076909
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2213	50-51	-0.5713450422745439
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2213	70-71	-0.5163352172596731
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2213	82-83	-0.41201036335488084
2213	84-85	-0.43157199905009236
2213	86-87	-0.38102349948665193
2213	88-89	-0.33763677013329385
2213	90-91	-0.42020399719717005
2213	92-93	-0.46207496948049
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2213	96-97	-0.351988487594884
2213	98-99	-0.43415281777278025
2213	100	-0.2981248353623336
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2214	2	-0.7578717040233087
2214	3	-0.6114788392158275
2214	4	-0.555655973258169
2214	5	-0.4983703229432095
2214	6	-0.5457984759917451
2214	7	-0.5012825489209476
2214	8	-0.6483798539307557
2214	9	-1.068437114318435
2214	10-11	-0.7197667110151826
2214	12-13	-0.7447473005145042
2214	14-15	-3.1902288205704608
2214	16-17	-1.4731994707523839
2214	18-19	-0.8417279165694183
2214	20-21	-0.7007049081475358
2214	22-23	-0.7368604579270865
2214	24-25	-0.6742353515851178
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2214	30-31	-0.7756087021608948
2214	32-33	-0.7915626771367315
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2214	36-37	-0.735082566493162
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2214	70-71	-0.4979806897395491
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2214	74-75	-0.6046220880751534
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2214	80-81	-0.5680311024309432
2214	82-83	-0.5286516889480382
2214	84-85	-0.5866495190187564
2214	86-87	-0.6344434059563753
2214	88-89	-0.6060548461512596
2214	90-91	-0.654558621148901
2214	92-93	-0.6183793192706588
2214	94-95	-0.5866493306368383
2214	96-97	-0.5868122501920112
2214	98-99	-0.5650822500963031
2214	100	-0.42321582059966545
2215	1	-0.7857769701628285
2215	2	-1.0077000910266527
2215	3	-0.7851214610207968
2215	4	-2.2641307373208903
2215	5	-0.9606305579262653
2215	6	-0.6904535022041784
2215	7	-0.6707932046374623
2215	8	-0.5778587653994123
2215	9	-0.8944909449618805
2215	10-11	-0.6632680379639027
2215	12-13	-0.7912719734064737
2215	14-15	-0.8548206805343952
2215	16-17	-0.8927412116511135
2215	18-19	-0.7870625687314501
2215	20-21	-0.867029500815832
2215	22-23	-0.8464261045265218
2215	24-25	-0.8724205046818838
2215	26-27	-0.8210966129871835
2215	28-29	-0.7857331397720486
2215	30-31	-3.407170814016439
2215	32-33	-1.3065796736699866
2215	34-35	-1.1233064563183106
2215	36-37	-1.0046504738670947
2215	38-39	-0.8289710036167364
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2215	82-83	-0.7779657973913885
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2215	86-87	-0.7083157255879726
2215	88-89	-0.7311765014865337
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2215	92-93	-0.7134860595095738
2215	94-95	-0.6780658216772046
2215	96-97	-0.9076616105770263
2215	98-99	-0.8303792794737461
2215	100	-0.45255477776054676
2216	1	-0.49875558824044575
2216	2	-0.7047372308426567
2216	3	-0.715172541965778
2216	4	-0.5175289431635761
2216	5	-0.49415585369798265
2216	6	-0.40957096566125983
2216	7	-0.5021469877949585
2216	8	-0.5663444265521775
2216	9	-0.7287354865354772
2216	10-11	-1.0592883929245005
2216	12-13	-0.7453362596516797
2216	14-15	-0.8110568246826872
2216	16-17	-0.8106896678373232
2216	18-19	-1.1108610679606414
2216	20-21	-1.166722443602012
2216	22-23	-0.9811798290576021
2216	24-25	-1.037811765129824
2216	26-27	-1.3312701724374776
2216	28-29	-1.2303290736503243
2216	30-31	-1.3546607638387016
2216	32-33	-1.6257987520067587
2216	34-35	-1.237149209768063
2216	36-37	-1.3585584805526096
2216	38-39	-1.2492323818390787
2216	40-41	-1.1390410169422083
2216	42-43	-1.1738205933797445
2216	44-45	-1.0894573510538024
2216	46-47	-1.1891548669374998
2216	48-49	-1.2012260557967451
2216	50-51	-1.213908612420056
2216	52-53	-1.2613907769549613
2216	54-55	-1.2357074000958619
2216	56-57	-1.2088750848192973
2216	58-59	-1.123499162572955
2216	60-61	-1.196692331655008
2216	62-63	-1.3029418953255671
2216	64-65	-1.200633939503632
2216	66-67	-1.191839349504896
2216	68-69	-1.1591368434545188
2216	70-71	-1.0651368961611354
2216	72-73	-1.0316666339791496
2216	74-75	-1.1160285461643085
2216	76-77	-1.0513430238814578
2216	78-79	-0.9736104447577176
2216	80-81	-1.0594361560297187
2216	82-83	-1.094111991063187
2216	84-85	-1.1090047103574001
2216	86-87	-1.1111355316846456
2216	88-89	-1.1027865007977766
2216	90-91	-1.1578477486823289
2216	92-93	-1.1365748129240743
2216	94-95	-1.1416587449999973
2216	96-97	-1.1566045495922879
2216	98-99	-1.1582467308911433
2216	100	-0.8472035181194926
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17.0
3	32.0
4	58.0
5	47.0
6	75.0
7	252.0
8	661.0
9	1004.0
10	1397.0
11	2489.0
12	4038.0
13	6271.0
14	8719.0
15	11620.0
16	14496.0
17	17517.0
18	20164.0
19	23188.0
20	27107.0
21	31380.0
22	36500.0
23	42725.0
24	49688.0
25	58071.0
26	65757.0
27	72618.0
28	82860.0
29	102180.0
30	121328.0
31	140218.0
32	173771.0
33	228410.0
34	334740.0
35	486516.0
36	786473.0
37	1423027.0
38	1310468.0
39	524647.0
40	1309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17808363049028	20.64792536210138	12.08618107058329	24.087809936825057
2	15.705532139602726	19.80094472368281	40.52676234113734	23.966760795577123
3	18.50141120985707	26.591344182419984	29.063984130952804	25.843260476770137
4	11.895870201930434	16.440174341774792	40.46156263604351	31.202392820251262
5	12.832795065613603	38.84784466722144	35.41011138366599	12.909248883498966
6	29.981100090635852	38.76289307062352	18.024573101919454	13.231433736821177
7	26.838738903929332	31.965521587965817	23.805304477579746	17.39043503052511
8	26.18797900172045	35.24206636232931	19.903724346790042	18.6662302891602
9	26.18934240253299	14.905307548833719	21.34446184145836	37.56088820717493
10-11	25.588023766048586	25.872176393244956	27.970649421001394	20.56915041970507
12-13	25.627690233235917	23.80448119730411	27.83999605912726	22.727832510332718
14-15	23.037442542042196	25.133911944872235	25.09756191598781	26.73108359709776
16-17	22.02402943946816	27.68846921430304	26.390326341958698	23.897175004270103
18-19	21.4360348350256	28.459425154302664	27.937847975360835	22.1666920353109
20-21	23.205105275130073	27.561004744057694	26.969096252904368	22.26479372790787
22-23	23.626418118431	27.24193593047501	26.610189846600445	22.52145610449355
24-25	23.073556012180774	27.207902242135717	27.501571214323295	22.216970531360214
26-27	22.988419238536963	27.479512105988878	26.404490312069605	23.127578343404554
28-29	22.035527442401992	28.003106141777966	27.046438944649132	22.91492747117091
30-31	22.887312209420354	27.64637470423844	27.026645868707867	22.43966721763334
32-33	23.343685758522156	27.7760408357049	26.05584030851526	22.824433097257682
34-35	23.23996533262305	27.589921024418253	27.073677513382815	22.09643612957588
36-37	22.869298789048113	27.1687205537252	26.710508182490265	23.251472474736417
38-39	23.03872181528452	27.356227241567726	27.297295600339634	22.307755342808118
40-41	22.63936427690394	26.445120311347768	27.961828215054258	22.95368719669404
42-43	22.61012902712664	26.72918214338501	27.253426291857174	23.40726253763117
44-45	22.82182049106901	27.638904397784202	27.08664638894817	22.45262872219861
46-47	22.758304216915967	27.398489703254064	26.64194052200235	23.201265557827618
48-49	23.486896030249397	27.022519188661597	26.825731601310586	22.66485317977842
50-51	23.019880692978358	26.812134994788835	26.92194550082044	23.24603881141237
52-53	22.50052352918491	27.446769299588397	26.705261965469855	23.34744520575683
54-55	22.512939667108945	27.335477982539828	26.736120475624897	23.415461874726326
56-57	23.604170349711328	26.743951836885316	26.49383486499689	23.15804294840646
58-59	22.497758925153537	27.213954519967203	27.32609078053809	22.962195774341172
60-61	22.52078420891199	27.85468804969372	26.609656173998868	23.01487156739542
62-63	22.904262500847274	27.138024565299744	26.24600333691884	23.711709596934146
64-65	22.10147733396274	27.343155929501293	26.727534369399663	23.827832367136303
66-67	22.8114836039483	26.954084679431272	26.649116556058733	23.585315160561695
68-69	22.29356033164292	27.055582387507044	26.95742574257426	23.693431538275778
70-71	22.26560028438578	26.654236058552172	27.141720041324806	23.93844361573724
72-73	22.868458611421254	26.753086906717822	27.55071188629139	22.827742595569536
74-75	23.079034052840495	27.03139277552953	27.002992130332558	22.886581041297422
76-77	22.772132885419907	26.957640270863596	26.72579186161449	23.544434982102004
78-79	22.65063878088246	26.91107783708069	26.77904702103509	23.659236361001764
80-81	22.206708126734014	27.27898283704551	27.392994668097415	23.121314368123056
82-83	23.04511567197369	26.278102962727136	28.40606962414809	22.270711741151082
84-85	23.25598450146824	26.798683146441515	27.096686630165756	22.848645721924488
86-87	21.92907168387977	27.49532824032237	27.543096349096487	23.032503726701375
88-89	23.142311066726247	26.27074104058127	27.25869425798177	23.32825363471072
90-91	22.722203666525225	26.30873411425044	27.46018555213986	23.508876667084472
92-93	23.088005269122	26.50700181596986	27.39716433718227	23.00782857772587
94-95	21.983439274589838	26.55010378124355	28.007178756098178	23.459278188068435
96-97	22.345064764354543	26.453224339679853	27.522252571282568	23.679458324683033
98-99	21.673748675594833	27.18433675254324	27.397473209623975	23.744441362237957
100	22.55608998933679	26.444367466466424	26.545317663831003	24.454224880365786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	952.0
1	667.0
2	443.5
3	563.0
4	555.5
5	472.0
6	458.5
7	467.0
8	510.5
9	554.0
10	507.0
11	457.5
12	442.5
13	471.5
14	553.5
15	928.5
16	1154.0
17	1311.5
18	1785.0
19	2267.5
20	2893.5
21	3692.5
22	5161.5
23	7253.5
24	9533.0
25	12419.0
26	16902.0
27	21462.0
28	27290.0
29	40000.0
30	57905.0
31	74257.5
32	95270.5
33	115210.0
34	128110.5
35	144838.0
36	160947.5
37	188038.0
38	199501.5
39	186160.0
40	174217.5
41	161858.0
42	160642.0
43	171343.0
44	187185.0
45	221690.5
46	254705.0
47	276747.0
48	290863.0
49	286343.5
50	273752.5
51	279695.0
52	282580.0
53	267789.0
54	253768.0
55	218735.0
56	190812.5
57	158061.5
58	123026.0
59	118689.0
60	99679.0
61	68904.5
62	51982.0
63	43875.5
64	32002.5
65	15560.5
66	8738.5
67	6168.0
68	4591.5
69	3478.5
70	2802.0
71	2200.5
72	1733.0
73	1563.5
74	1242.0
75	915.0
76	640.5
77	420.5
78	252.5
79	162.0
80	116.0
81	73.0
82	52.5
83	38.0
84	31.0
85	27.5
86	22.0
87	20.0
88	17.5
89	9.5
90	9.5
91	13.0
92	10.5
93	9.5
94	9.5
95	8.5
96	9.5
97	11.0
98	9.0
99	6.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061801354124173875
2	0.008016950860598747
3	0.00806524574530115
4	0.00949799399147241
5	0.00394408225069617
6	0.002704513543334517
7	0.0010785857583536468
8	0.0014649448359728633
9	7.083249756352307E-4
10-11	5.714894689784248E-4
12-13	2.575727184128112E-4
14-15	2.1732698116080942E-4
16-17	6.600300909328286E-4
18-19	0.0016339769324312707
20-21	0.0010785857583536468
22-23	0.00138445336146886
24-25	0.0013200601818656572
26-27	0.0039118856608945695
28-29	0.004402883655368991
30-31	0.00532853561216503
32-33	0.006616399204229087
34-35	0.003775050154237763
36-37	0.008467703117821166
38-39	0.006503711139923481
40-41	0.006302482453663473
42-43	0.014424072231117423
44-45	0.02003432800404647
46-47	0.01831985959711119
48-49	0.02148317454511853
50-51	0.018335957892011995
52-53	0.025475551680517104
54-55	0.019116725194700826
56-57	0.015196790386355857
58-59	0.01755519058932316
60-61	0.01598560683649509
62-63	0.011574674033675701
64-65	0.0115424774438741
66-67	0.013860631909589399
68-69	0.005441223676470636
70-71	0.00896675025974599
72-73	0.008419408233118764
74-75	0.011453936821919697
76-77	0.010745611846284467
78-79	0.012685456381830948
80-81	0.010310957883962846
82-83	0.00668884153128269
84-85	0.010769759288635666
86-87	0.009353109337365205
88-89	0.008234277841759556
90-91	0.012999373132396562
92-93	0.008958701112295587
94-95	0.007332773327314717
96-97	0.00948994484402201
98-99	0.008998946849547591
100	0.2689059180229748
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6211838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.10916719303794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11247657309366	44.788458641823055
2	15.624893188832075	19.408982068770623
3	5.939003327996172	11.065996519755691
4	2.594480253813518	6.445640314525572
5	1.3737969125373686	4.266269106503138
6	0.7625916914779478	2.841836091961528
7	0.452613765609787	1.9678024829490122
8	0.30446209985257167	1.5127909970949431
9	0.19435301606980968	1.0863993572595898
>10	0.6265386495578309	5.79116045967981
>50	0.011438790884258636	0.45982869205138743
>100	0.0032285340780525917	0.2990058914954462
>500	8.213080323928516E-5	0.029688261049639134
>1k	4.106539378834357E-5	0.036141115080544614
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.6098294900800696E-5
7	0.0	0.0	0.0	0.0	1.6098294900800696E-5
8	0.0	0.0	0.0	0.0	1.6098294900800696E-5
9	0.0	0.0	0.0	0.0	1.6098294900800696E-5
10-11	0.0	0.0	0.0	0.0	1.6098294900800696E-5
12-13	0.0	0.0	0.0	0.0	1.2878635920640557E-4
14-15	0.0	0.0	0.0	0.0	1.3683550665680592E-4
16-17	0.0	0.0	0.0	0.0	1.4488465410720627E-4
18-19	0.0	0.0	0.0	0.0	1.6098294900800697E-4
20-21	0.0	0.0	0.0	2.4147442351201043E-5	1.8513039135920802E-4
22-23	0.0	0.0	0.0	8.049147450400348E-5	1.9317953880960837E-4
24-25	0.0	0.0	0.0	1.2878635920640557E-4	1.9317953880960837E-4
26-27	0.0	0.0	0.0	4.266048148712185E-4	2.3342527606161012E-4
28-29	0.0	0.0	0.0	0.0026803661009833163	2.4147442351201047E-4
30-31	0.0	0.0	0.0	0.010359252768665249	2.4147442351201047E-4
32-33	0.0	0.0	0.0	0.024896013064088278	2.5757271841281114E-4
34-35	0.0	0.0	0.0	0.047731444380874066	2.7367101331361184E-4
36-37	0.0	0.0	0.0	0.08730910239449258	2.7367101331361184E-4
38-39	0.0	0.0	0.0	0.14388655982335663	2.7367101331361184E-4
40-41	0.0	0.0	0.0	0.20464152477897846	2.7367101331361184E-4
42-43	0.0	0.0	0.0	0.2694130143123501	2.817201607640122E-4
44-45	0.0	0.0	0.0	0.34919616384071833	2.978184556648129E-4
46-47	0.0	0.0	0.0	0.452289644385446	3.139167505656136E-4
48-49	0.0	0.0	0.0	0.5700406224373527	3.2196589801601394E-4
50-51	0.0	0.0	0.0	0.6946655724119013	3.5416248781761534E-4
52-53	0.0	0.0	0.0	0.8165296648109626	4.1855566742081814E-4
54-55	0.0	0.0	0.0	0.9516587522082836	4.427031097720192E-4
56-57	0.0	0.0	0.0	1.11886369219545	4.5075225722241954E-4
58-59	0.0	0.0	0.0	1.3185630404398827	4.5075225722241954E-4
60-61	0.0	0.0	0.0	1.5272452372389622	4.5075225722241954E-4
62-63	0.0	0.0	0.0	1.7476550418732748	4.5075225722241954E-4
64-65	0.0	0.0	0.0	1.9771684322739904	4.5075225722241954E-4
66-67	8.049147450400348E-6	0.0	0.0	2.23912793604727	4.5075225722241954E-4
68-69	1.6098294900800696E-5	0.0	0.0	2.5349582522918337	4.5075225722241954E-4
70-71	1.6098294900800696E-5	0.0	0.0	2.8644983980586742	4.5075225722241954E-4
72-73	1.6098294900800696E-5	0.0	0.0	3.208333829697426	4.829488470240209E-4
74-75	1.6098294900800696E-5	0.0	0.0	3.5454884689523456	5.392928791768234E-4
76-77	1.6098294900800696E-5	0.0	0.0	3.9352040410583795	5.55391174077624E-4
78-79	1.6098294900800696E-5	0.0	0.0	4.3567217947409445	5.956369113296258E-4
80-81	1.6098294900800696E-5	0.0	0.0	4.821318585578053	5.956369113296258E-4
82-83	1.6098294900800696E-5	0.0	0.0	5.303486665299385	6.278335011312272E-4
84-85	1.6098294900800696E-5	0.0	0.0	5.7760601612598395	6.278335011312272E-4
86-87	1.6098294900800696E-5	0.0	0.0	6.281602965177134	6.68079238383229E-4
88	1.6098294900800696E-5	0.0	0.0	6.68985572386144	7.083249756352307E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6740	0.0	40.967205	94
GTACTAG	6665	0.0	36.541496	1
CAACGCA	11295	0.0	33.61774	5
GTATCAA	13110	0.0	32.420704	1
GCATCGT	6785	0.0	32.343422	8
TAGGCAT	8000	0.0	31.955994	5
CTGTGCG	9185	0.0	31.924517	9
ATCAACG	12240	0.0	31.13944	3
TAGTACT	9775	0.0	30.385626	4
GGTATCA	8625	0.0	29.818441	1
CTAGGCA	9075	0.0	29.2079	4
ACTGTGC	10690	0.0	29.012499	8
GTACTGT	10765	0.0	28.374308	6
TACTGTG	11535	0.0	27.905636	7
CTAGTAC	11130	0.0	27.826694	3
CTAACGC	3535	0.0	27.786142	3
TAACGCC	3540	0.0	27.613914	4
TCTAACG	3590	0.0	27.49136	2
AACGCAG	14570	0.0	27.479319	6
CGCCTAC	3615	0.0	26.907972	7
>>END_MODULE