FastQCFastQC Report
Mon 16 Jan 2017
ERR1146670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6211838
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152220.24504824497998823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135370.21792261807213903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.13569252771884907No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121000.045.7623751
GTATCAA208250.039.4662061
GGGCACC67200.039.40312294
GTGGTAT28950.035.2637631
GTACTAG66950.033.0969351
CAACGCA240550.032.705435
ATCAACG242800.032.4713823
CTGTGCG91050.030.563219
TATCAAC271800.030.186622
TAGGCAT82150.029.5769085
TAGTACT97050.029.3248184
AACGCAG279950.029.0929586
ACTGTGC106600.028.040598
GTACTGT104850.027.5661746
TCAACGC293700.027.1321954
CTAGTAC109250.026.9973753
CTAGGCA92600.026.7716484
ACGCAGA294050.026.7231947
CAGTACG11150.026.559689
CGCAGAG297600.026.421068
Produced by FastQC (version 0.11.5)