##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146670_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6211838 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17171439435478 33.0 31.0 34.0 30.0 34.0 2 32.22990441798385 33.0 31.0 34.0 30.0 34.0 3 32.39915190962804 34.0 31.0 34.0 30.0 34.0 4 35.99718617903429 37.0 35.0 37.0 35.0 37.0 5 35.90195816439515 37.0 35.0 37.0 35.0 37.0 6 35.944787516995774 37.0 35.0 37.0 35.0 37.0 7 35.873405262661386 37.0 35.0 37.0 35.0 37.0 8 35.88326353649274 37.0 35.0 37.0 35.0 37.0 9 37.603054361688116 39.0 37.0 39.0 35.0 39.0 10-11 37.54491392080733 39.0 37.0 39.0 35.0 39.0 12-13 37.50481588541105 39.0 37.0 39.0 34.5 39.0 14-15 38.74288672692366 40.0 38.0 41.0 34.0 41.0 16-17 38.73951751478387 40.0 38.0 41.0 34.0 41.0 18-19 38.805737850858314 40.0 38.0 41.0 34.5 41.0 20-21 38.80598568410831 40.0 38.0 41.0 34.0 41.0 22-23 38.76817690673839 40.0 38.0 41.0 34.0 41.0 24-25 38.69299191318254 40.0 38.0 41.0 34.0 41.0 26-27 38.555462891981406 40.0 38.0 41.0 34.0 41.0 28-29 38.500593866098896 40.0 38.0 41.0 34.0 41.0 30-31 38.371460427654426 40.0 38.0 41.0 34.0 41.0 32-33 38.22883291547526 40.0 37.0 41.0 34.0 41.0 34-35 38.125578451981525 40.0 37.0 41.0 33.0 41.0 36-37 38.11402333737615 40.0 37.0 41.0 33.0 41.0 38-39 38.01945994084198 40.0 37.0 41.0 33.0 41.0 40-41 37.90489038831985 40.0 37.0 41.0 33.0 41.0 42-43 37.772372685829865 40.0 37.0 41.0 33.0 41.0 44-45 37.569715195405934 40.0 36.5 41.0 32.5 41.0 46-47 37.32542646797937 40.0 36.0 41.0 31.5 41.0 48-49 37.0581217668587 40.0 36.0 41.0 31.0 41.0 50-51 36.85219205652176 39.0 35.0 41.0 31.0 41.0 52-53 36.67276239335282 39.0 35.0 41.0 31.0 41.0 54-55 36.48904293705019 39.0 35.0 41.0 30.0 41.0 56-57 36.27238870363328 38.5 35.0 41.0 30.0 41.0 58-59 36.00137036735343 38.0 35.0 40.0 30.0 41.0 60-61 35.82614984486074 37.0 35.0 40.0 30.0 41.0 62-63 35.61081188852639 37.0 35.0 40.0 30.0 41.0 64-65 35.36337401265133 36.0 34.5 40.0 29.0 41.0 66-67 35.11093343065289 36.0 34.0 39.0 29.0 41.0 68-69 34.85776327392955 35.5 34.0 39.0 29.0 41.0 70-71 34.63306230780648 35.0 34.0 39.0 29.0 41.0 72-73 34.38792777596583 35.0 34.0 37.5 29.0 40.5 74-75 34.125485484328465 35.0 34.0 37.0 29.0 40.0 76-77 33.876643917629536 35.0 34.0 37.0 29.0 39.0 78-79 33.64602650616452 35.0 34.0 36.0 29.0 39.0 80-81 33.505861711139275 35.0 34.0 36.0 29.0 38.5 82-83 33.33931535239651 35.0 34.0 36.0 29.0 37.0 84-85 33.20311291118668 35.0 33.0 35.0 29.0 37.0 86-87 33.05743501359823 35.0 33.0 35.0 29.0 36.5 88-89 32.94958118675986 35.0 33.0 35.0 29.0 36.0 90-91 32.8783371362872 35.0 33.0 35.0 29.0 36.0 92-93 32.81055357528641 35.0 33.0 35.0 29.0 36.0 94-95 32.761057596801464 35.0 33.0 35.0 29.0 35.5 96-97 32.673137886081385 35.0 33.0 35.0 28.5 35.0 98-99 32.59924180250677 35.0 33.0 35.0 27.0 35.0 100 31.38217142816667 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3744301774586134 1101 2 0.4635064346366846 1101 3 0.38234609281164467 1101 4 0.2474284110127556 1101 5 0.24529577224112842 1101 6 0.2519141711453301 1101 7 0.28503281881751263 1101 8 0.2940929794021514 1101 9 0.3929370336607221 1101 10-11 0.4080793608440203 1101 12-13 0.40031831802572526 1101 14-15 0.5258900729610403 1101 16-17 0.5682390852601955 1101 18-19 0.5403277701320661 1101 20-21 0.5501657325313687 1101 22-23 0.5385706057011248 1101 24-25 0.4909976433421761 1101 26-27 0.5421576773532308 1101 28-29 0.551371680212597 1101 30-31 0.5749806868270042 1101 32-33 0.6111895530424079 1101 34-35 0.645984640872129 1101 36-37 0.559792683566684 1101 38-39 0.627515908836692 1101 40-41 0.5830360269919126 1101 42-43 0.5296810247757122 1101 44-45 0.5638601890732318 1101 46-47 0.603178321224398 1101 48-49 0.5584077240961989 1101 50-51 0.5553831896969257 1101 52-53 0.6154290584955859 1101 54-55 0.6214558692713794 1101 56-57 0.5975254784535835 1101 58-59 0.6088087442853976 1101 60-61 0.5797556718869288 1101 62-63 0.5689923540164443 1101 64-65 0.5730698464099149 1101 66-67 0.5581371376446143 1101 68-69 0.5157501532024611 1101 70-71 0.540155806080179 1101 72-73 0.5360070075896743 1101 74-75 0.548134911265663 1101 76-77 0.4643148949214648 1101 78-79 0.4730289592403949 1101 80-81 0.4889152668645451 1101 82-83 0.4578694568386936 1101 84-85 0.4176952901608644 1101 86-87 0.40630577298277615 1101 88-89 0.4869773101373269 1101 90-91 0.4547913525154783 1101 92-93 0.42241029364409854 1101 94-95 0.4387327696152852 1101 96-97 0.4376556810328722 1101 98-99 0.4681187009886614 1101 100 0.8149958648135538 1102 1 0.36402071466001473 1102 2 0.3921447537872069 1102 3 0.3240261974343426 1102 4 0.21951724628481628 1102 5 0.25047068563192454 1102 6 0.22205463183237129 1102 7 0.2651980860229983 1102 8 0.2782673989495521 1102 9 0.3174219543642778 1102 10-11 0.297030069492223 1102 12-13 0.3513375340844789 1102 14-15 0.4639698218005037 1102 16-17 0.43553739343165887 1102 18-19 0.41184977340036966 1102 20-21 0.44262311441811164 1102 22-23 0.4592511296817534 1102 24-25 0.47813212557806395 1102 26-27 0.4807391509123633 1102 28-29 0.5056612091841473 1102 30-31 0.5278909840136876 1102 32-33 0.4791602751726316 1102 34-35 0.5259882198272336 1102 36-37 0.5274998994691558 1102 38-39 0.5522229359844246 1102 40-41 0.5081000364491857 1102 42-43 0.48993260697641716 1102 44-45 0.4961947898505983 1102 46-47 0.5726836503894006 1102 48-49 0.5110157913484699 1102 50-51 0.5051106626282476 1102 52-53 0.5142134565065177 1102 54-55 0.4788896973136616 1102 56-57 0.42305836517588347 1102 58-59 0.46923553011610153 1102 60-61 0.5013093751525588 1102 62-63 0.5061721826538204 1102 64-65 0.4570196065198644 1102 66-67 0.45848757666814066 1102 68-69 0.42963800779158134 1102 70-71 0.37881373841291577 1102 72-73 0.46397025258626456 1102 74-75 0.4552640040255582 1102 76-77 0.3871780097749564 1102 78-79 0.3972482376191806 1102 80-81 0.3821225260051193 1102 82-83 0.34198809963729815 1102 84-85 0.3443927984156119 1102 86-87 0.3827123035316191 1102 88-89 0.34766251209100574 1102 90-91 0.35853695495479343 1102 92-93 0.3528447408368791 1102 94-95 0.355754889971891 1102 96-97 0.32675476504429923 1102 98-99 0.40003843043331955 1102 100 0.6556485464768897 1103 1 0.2830836878563119 1103 2 0.34016540577239596 1103 3 0.2531202659113987 1103 4 0.21032299163989165 1103 5 0.1954383343022954 1103 6 0.2717654177407596 1103 7 0.25583512828261235 1103 8 0.2223868815332608 1103 9 0.29854657880515134 1103 10-11 0.3065322636573953 1103 12-13 0.2738547569691434 1103 14-15 0.3593577087542599 1103 16-17 0.32235394475296175 1103 18-19 0.30340177884560404 1103 20-21 0.3367970642208462 1103 22-23 0.3611134078950897 1103 24-25 0.3632943027027906 1103 26-27 0.40080819952119384 1103 28-29 0.44633523724010615 1103 30-31 0.3813860411974801 1103 32-33 0.4369349652703747 1103 34-35 0.43105437070929753 1103 36-37 0.3971384707671177 1103 38-39 0.3798551219317261 1103 40-41 0.40654026692056533 1103 42-43 0.4077160924910146 1103 44-45 0.47142584954587363 1103 46-47 0.45312335794268677 1103 48-49 0.4406394079267173 1103 50-51 0.4351020745030212 1103 52-53 0.4292265881361388 1103 54-55 0.38368729792722434 1103 56-57 0.35973541871585013 1103 58-59 0.37400254945013245 1103 60-61 0.3373847231206284 1103 62-63 0.3523038967756591 1103 64-65 0.340714503589453 1103 66-67 0.3449806672207032 1103 68-69 0.3149545454301048 1103 70-71 0.35247802319890553 1103 72-73 0.3324430903629647 1103 74-75 0.28003429723482043 1103 76-77 0.31205972159071393 1103 78-79 0.2844914727377983 1103 80-81 0.25808308181724726 1103 82-83 0.2682065157222624 1103 84-85 0.29602145845796457 1103 86-87 0.2922693864173169 1103 88-89 0.26065382654640246 1103 90-91 0.23816249199127526 1103 92-93 0.23660166524825144 1103 94-95 0.21235590079656674 1103 96-97 0.2955289277142583 1103 98-99 0.3190874582074201 1103 100 0.5506157646776408 1104 1 0.22889938058848003 1104 2 0.27383475282749004 1104 3 0.1808036145095997 1104 4 0.17385194398655557 1104 5 0.17117841746006945 1104 6 0.1690599672630384 1104 7 0.19483540793904552 1104 8 0.1694894982755173 1104 9 0.2196018855939883 1104 10-11 0.269404731495932 1104 12-13 0.2900983652724278 1104 14-15 0.2902027367965303 1104 16-17 0.3076510840800637 1104 18-19 0.3075068622487933 1104 20-21 0.3685862673872151 1104 22-23 0.38762268932647004 1104 24-25 0.34345452532405574 1104 26-27 0.34718415695405724 1104 28-29 0.38374500729907624 1104 30-31 0.33170032973036 1104 32-33 0.36346408237474037 1104 34-35 0.4299590981737751 1104 36-37 0.35403885385666456 1104 38-39 0.3506391847742023 1104 40-41 0.44433470523018315 1104 42-43 0.42717329443477325 1104 44-45 0.40818690131014534 1104 46-47 0.3944636565125563 1104 48-49 0.3875126333984795 1104 50-51 0.3561482085510903 1104 52-53 0.29784471892339326 1104 54-55 0.36129512207018166 1104 56-57 0.3533554410398665 1104 58-59 0.307163927945858 1104 60-61 0.31025070104804087 1104 62-63 0.29193979947229565 1104 64-65 0.28262104460117143 1104 66-67 0.23325601783886185 1104 68-69 0.28385448003143665 1104 70-71 0.2855288559212852 1104 72-73 0.2707118788036027 1104 74-75 0.31523989467039826 1104 76-77 0.2836596248507348 1104 78-79 0.27096027964758207 1104 80-81 0.27239880796656735 1104 82-83 0.22011350245764305 1104 84-85 0.22333081022540568 1104 86-87 0.2291529333746638 1104 88-89 0.3181353370170825 1104 90-91 0.29332024955213143 1104 92-93 0.2698764583480866 1104 94-95 0.2864695848718952 1104 96-97 0.2437346195811756 1104 98-99 0.25219087807678164 1104 100 0.48701367775968407 1105 1 0.2195322151835981 1105 2 0.2305843574523081 1105 3 0.18106536694075714 1105 4 0.19692267198242774 1105 5 0.2046510922521847 1105 6 0.15498779962879183 1105 7 0.18956913318989166 1105 8 0.16882837053098854 1105 9 0.1628314551944925 1105 10-11 0.2311970876502798 1105 12-13 0.2083500256475972 1105 14-15 0.31163025829138746 1105 16-17 0.3170736701603545 1105 18-19 0.2506369128182584 1105 20-21 0.31087316127158715 1105 22-23 0.2828926389575912 1105 24-25 0.2853927635569633 1105 26-27 0.3116312808432298 1105 28-29 0.3444215080984847 1105 30-31 0.35802697397165417 1105 32-33 0.36538258592396033 1105 34-35 0.2384069812452836 1105 36-37 0.30203334793849734 1105 38-39 0.36498603974944643 1105 40-41 0.3594405841341697 1105 42-43 0.27510314929619994 1105 44-45 0.33448879371822926 1105 46-47 0.35451556641410065 1105 48-49 0.37130605748633627 1105 50-51 0.3509503520673647 1105 52-53 0.30214777595200104 1105 54-55 0.36322695940312855 1105 56-57 0.27240117382108764 1105 58-59 0.2481719410256389 1105 60-61 0.2535894994092729 1105 62-63 0.2811419247725766 1105 64-65 0.270100262702627 1105 66-67 0.2685811882525968 1105 68-69 0.24801709858415677 1105 70-71 0.2776221501038947 1105 72-73 0.2172252687259686 1105 74-75 0.2525090521152151 1105 76-77 0.24281312833773683 1105 78-79 0.21248484008971502 1105 80-81 0.19475919401995156 1105 82-83 0.19525532947046287 1105 84-85 0.20570997571653749 1105 86-87 0.23445580195247828 1105 88-89 0.18068141777585822 1105 90-91 0.19810938681712997 1105 92-93 0.21448499760403905 1105 94-95 0.2158298540894208 1105 96-97 0.23173459601342472 1105 98-99 0.21587970284802083 1105 100 0.4315525223538188 1106 1 0.14390910818060831 1106 2 0.18659236374960386 1106 3 0.1607238862569318 1106 4 0.14791400941611244 1106 5 0.10568971791288106 1106 6 0.16496038595987983 1106 7 0.1802278665708954 1106 8 0.12219720423129132 1106 9 0.09082435925549248 1106 10-11 0.16102496426571378 1106 12-13 0.18369461856222813 1106 14-15 0.2209298258118224 1106 16-17 0.21453930004424393 1106 18-19 0.21290405574068672 1106 20-21 0.21522046740403766 1106 22-23 0.21707510967387122 1106 24-25 0.21658938836993258 1106 26-27 0.24151525766194482 1106 28-29 0.19194344820405718 1106 30-31 0.24011467167428435 1106 32-33 0.25280464105803446 1106 34-35 0.21119190067496874 1106 36-37 0.19858632141860255 1106 38-39 0.24189141794904145 1106 40-41 0.26137592789118713 1106 42-43 0.2165952170001475 1106 44-45 0.2197625640297005 1106 46-47 0.24662630434487198 1106 48-49 0.3072353260157712 1106 50-51 0.34403604599653903 1106 52-53 0.26755384730029164 1106 54-55 0.25710981830819435 1106 56-57 0.2663265949893443 1106 58-59 0.21532776803786646 1106 60-61 0.26211590012975705 1106 62-63 0.28041452988876614 1106 64-65 0.26858761891314487 1106 66-67 0.26691360916044005 1106 68-69 0.19966857553251316 1106 70-71 0.2329138727003368 1106 72-73 0.22309027448355323 1106 74-75 0.20930597439340914 1106 76-77 0.21507643792953246 1106 78-79 0.20728826462557493 1106 80-81 0.17984634813778655 1106 82-83 0.18634202757348106 1106 84-85 0.26026906176571174 1106 86-87 0.1812695874987469 1106 88-89 0.17418978198409718 1106 90-91 0.21388378096022365 1106 92-93 0.22346950233644947 1106 94-95 0.23941834020773456 1106 96-97 0.20546039788017367 1106 98-99 0.17299966311562542 1106 100 0.4188949442279615 1107 1 0.10311450098851083 1107 2 0.1490706025672921 1107 3 0.13698417445306887 1107 4 0.1100182947837709 1107 5 0.04209708427615766 1107 6 0.037210061616512746 1107 7 0.10751249156847109 1107 8 0.09585238456305945 1107 9 0.07744350301694425 1107 10-11 0.14672021853746742 1107 12-13 0.04897246208888362 1107 14-15 0.15256170333675811 1107 16-17 0.14645870641596304 1107 18-19 0.17565121962496022 1107 20-21 0.16627013875472585 1107 22-23 0.07796717206973369 1107 24-25 0.05223444518832565 1107 26-27 0.1473783066765293 1107 28-29 0.11327958545313521 1107 30-31 0.09616046025010405 1107 32-33 0.18647616542208567 1107 34-35 0.13889807488531147 1107 36-37 0.11149795407412455 1107 38-39 0.08863434475689047 1107 40-41 0.17353275365307042 1107 42-43 0.14251326114717955 1107 44-45 0.16965659563132363 1107 46-47 0.26595551186051836 1107 48-49 0.24497881827371515 1107 50-51 0.20613724492719143 1107 52-53 0.21276032131098788 1107 54-55 0.26859265225958495 1107 56-57 0.1505244611270271 1107 58-59 0.17884873017471392 1107 60-61 0.21096791260458758 1107 62-63 0.172164013218989 1107 64-65 0.1478435775379836 1107 66-67 0.1478318712249873 1107 68-69 0.13966948475951568 1107 70-71 0.132799130829099 1107 72-73 0.12508879757923097 1107 74-75 0.1573831732685136 1107 76-77 0.16286176270873653 1107 78-79 0.12536268101275994 1107 80-81 0.05405975432748278 1107 82-83 0.08161562258194266 1107 84-85 0.07318127087639681 1107 86-87 0.08971139994015687 1107 88-89 0.08932932103483182 1107 90-91 0.08399109049026521 1107 92-93 -0.009921497984443306 1107 94-95 0.026214008077332096 1107 96-97 0.052665897201265466 1107 98-99 0.11582801939915299 1107 100 0.13372927974897308 1108 1 0.0857860821398333 1108 2 0.11463330043582687 1108 3 0.09227140736715 1108 4 0.07281154427321468 1108 5 0.07468584967690362 1108 6 0.0846459732096605 1108 7 0.05708788882808591 1108 8 0.03343984743212047 1108 9 0.012849350806952486 1108 10-11 0.019507315928024127 1108 12-13 0.104468175362598 1108 14-15 0.09981865485714536 1108 16-17 0.06822134005303582 1108 18-19 0.10190197211516505 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-0.3559573347451064 2215 7 -0.4205003186469227 2215 8 -0.4000522659775996 2215 9 -0.4526267215351183 2215 10-11 -0.48519384344704264 2215 12-13 -0.49861070611067504 2215 14-15 -0.6527266344445835 2215 16-17 -0.6448657740337893 2215 18-19 -0.6012222653137442 2215 20-21 -0.6187926144546907 2215 22-23 -0.656973761210466 2215 24-25 -0.6598068289954284 2215 26-27 -0.6881765089924698 2215 28-29 -0.6081587599121647 2215 30-31 -0.6479950984109095 2215 32-33 -0.675458215429309 2215 34-35 -0.6641941317102749 2215 36-37 -0.8008729230733564 2215 38-39 -0.8968064294311873 2215 40-41 -0.8868838970609332 2215 42-43 -0.8234960501003954 2215 44-45 -0.7723553269510077 2215 46-47 -0.893878139113994 2215 48-49 -0.907909108083814 2215 50-51 -0.8999333019228715 2215 52-53 -0.8697108828510522 2215 54-55 -0.8714498627287242 2215 56-57 -0.9105268983408621 2215 58-59 -0.8355215075494868 2215 60-61 -0.8943530052567894 2215 62-63 -0.8740889985750115 2215 64-65 -0.77709518563762 2215 66-67 -0.8695139258998807 2215 68-69 -0.8587300427376761 2215 70-71 -0.8351377825441375 2215 72-73 -0.7798603513878604 2215 74-75 -0.8086067911381818 2215 76-77 -0.7772882962767298 2215 78-79 -0.7905669609623374 2215 80-81 -0.8209875486984615 2215 82-83 -0.7769264526424919 2215 84-85 -0.7461215264079755 2215 86-87 -0.6761254657869102 2215 88-89 -0.7358989299390686 2215 90-91 -0.682026734385552 2215 92-93 -0.6519297996527698 2215 94-95 -0.6387852901414703 2215 96-97 -0.656948911473549 2215 98-99 -0.7410466992570548 2215 100 -0.3718663357599752 2216 1 -0.3970834934963996 2216 2 -0.24624067818305662 2216 3 -0.30874233123767425 2216 4 -0.37347285122928753 2216 5 -0.39400039973661194 2216 6 -0.36270988565750173 2216 7 -0.4109667475731129 2216 8 -0.4165751016569885 2216 9 -0.48516169248269136 2216 10-11 -0.554861910896129 2216 12-13 -0.4944006488999406 2216 14-15 -0.6883915808457601 2216 16-17 -0.6626403545577944 2216 18-19 -0.6514051639192999 2216 20-21 -0.6267671199601423 2216 22-23 -0.6409995531986397 2216 24-25 -0.6891517260530051 2216 26-27 -0.743732693034346 2216 28-29 -0.636794074712931 2216 30-31 -0.6478497499699145 2216 32-33 -0.6114800224204728 2216 34-35 -0.5767215526441234 2216 36-37 -0.820512449671412 2216 38-39 -0.9532619683098957 2216 40-41 -1.0073035175375793 2216 42-43 -0.9728807226184344 2216 44-45 -0.9930747217722953 2216 46-47 -0.9070926394655601 2216 48-49 -0.9542958690543273 2216 50-51 -0.9421128452292749 2216 52-53 -0.9029181993768205 2216 54-55 -0.8897109916161554 2216 56-57 -0.7813414202457167 2216 58-59 -0.840057644664796 2216 60-61 -0.820966849086993 2216 62-63 -0.8730943394668955 2216 64-65 -0.7810864478301767 2216 66-67 -0.8546689215301555 2216 68-69 -0.8556387260076548 2216 70-71 -0.8689056619198396 2216 72-73 -0.8630491177234347 2216 74-75 -0.8392838353709564 2216 76-77 -0.8865695520523644 2216 78-79 -0.8335689687262473 2216 80-81 -0.8691925508801432 2216 82-83 -0.8113753143613849 2216 84-85 -0.8137771685462241 2216 86-87 -0.8472617287690838 2216 88-89 -0.7752155014968238 2216 90-91 -0.7974004396204393 2216 92-93 -0.8509538633792815 2216 94-95 -0.8050741335491551 2216 96-97 -0.8086284737708382 2216 98-99 -0.8059653637378617 2216 100 -0.2922913536468492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 5.0 12 16.0 13 82.0 14 297.0 15 924.0 16 2223.0 17 4598.0 18 8376.0 19 13232.0 20 19069.0 21 27575.0 22 38735.0 23 46106.0 24 47907.0 25 46932.0 26 48401.0 27 56639.0 28 71420.0 29 89406.0 30 111929.0 31 140018.0 32 177333.0 33 229167.0 34 326770.0 35 483169.0 36 779290.0 37 1490923.0 38 1401420.0 39 548324.0 40 1550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.163458866763754 21.57065267960948 13.243938428529528 25.02195002509724 2 14.34049894611977 20.629906757519965 39.817015094346914 25.21257920201335 3 17.17060232414303 26.82985293563676 29.900361213540982 26.09918352667922 4 11.57197978612428 17.21598689275631 39.10096206369478 32.111071257424626 5 12.281739435616364 38.905153379467784 35.483707380841025 13.329399804074827 6 29.833578993392614 39.19664009159289 17.41969684969146 13.550084065323038 7 25.99887150898245 32.61472656630448 23.83949348300462 17.546908441708446 8 26.435750578170264 34.94564732692643 19.533268575259047 19.085333519644266 9 26.110987780265322 14.213235150890146 21.39573205156542 38.28004501727911 10-11 25.54728568259507 25.95746218752002 27.666038618521604 20.829213511363303 12-13 25.807536692529716 23.796106231616708 28.162328514511763 22.234028561341816 14-15 23.041740488005303 24.823490895151334 25.913984579688687 26.220784037154676 16-17 21.46464540768771 27.301033929088298 27.239691376368796 23.994629286855197 18-19 20.910386529968445 27.252313889907555 29.936537260976387 21.900762319147617 20-21 22.471674245207296 26.499346892175872 29.25391808350443 21.775060779112398 22-23 22.780238312718392 26.145345387307266 29.000120415245856 22.074295884728482 24-25 22.313910955179452 26.15458580858033 29.796390375924165 21.73511286031606 26-27 22.300686205918442 26.382948170895638 28.696353639615204 22.620011983570723 28-29 21.442067549089337 26.903937288770248 29.335536438651488 22.318458723488927 30-31 22.351057770663047 26.523333351578067 29.22230103231926 21.903307845439627 32-33 22.821152129208777 26.712496365809923 28.416677962303588 22.049673542677706 34-35 22.631103708757376 26.584675904297566 29.161055069369162 21.623165317575893 36-37 22.3752615570464 26.118445136528027 28.788733704903446 22.717559601522126 38-39 22.46668377378805 26.27406735333407 29.49657573169165 21.76267314118623 40-41 22.019247765315196 25.536435431831933 29.96876286857449 22.475553934278388 42-43 22.24134789091409 25.727119734931915 29.057398148502905 22.97413422565109 44-45 22.47656812685714 26.62837472580579 28.74285356443616 22.152203582900906 46-47 22.469718302376847 26.551867579289734 27.992962791367066 22.98545132696635 48-49 23.40882038456251 26.383592102691665 27.832664019892338 22.374923492853487 50-51 22.725463864318417 26.34564037246303 27.752687690825162 23.17620807239339 52-53 22.153338512691413 26.94191316644124 27.539369185094653 23.365379135772695 54-55 22.235045408460426 26.900291024975214 27.41925980683978 23.445403759724577 56-57 23.332007370443336 26.295445888962334 27.179805719337818 23.19274102125651 58-59 22.174901269125584 26.740161845869725 28.00729929122404 23.077637593780647 60-61 22.340135077572853 27.34148894417401 27.23840351277673 23.079972465476402 62-63 22.745168177277 26.599945137010977 26.859505994844042 23.795380690867983 64-65 21.94545651383871 26.815653834838592 27.319748238543134 23.919141412779563 66-67 22.551749456701838 26.439767292352002 27.283874627253475 23.724608623692685 68-69 22.16030907438346 26.34655797527238 27.639774250390946 23.853358699953215 70-71 22.085079269204595 26.020616867201447 27.72096750591253 24.17333635768143 72-73 22.687541619280484 25.99768553938628 28.069962488416227 23.24481035291701 74-75 22.814383533692226 26.40995303865869 27.515306927777832 23.260356499871254 76-77 22.515533462232515 26.387531599979813 27.097008346089236 23.99992659169844 78-79 22.264544653142806 26.449024088852312 27.255194760454078 24.031236497550804 80-81 21.88571240911305 26.8899720179438 27.78315371392493 23.441161859018216 82-83 22.697686256467087 25.819008802225685 28.817155244550808 22.666149696756417 84-85 22.968218102275042 26.38763277471177 27.320359932116713 23.32378919089648 86-87 21.562072288427352 26.982690147424965 27.824188267627072 23.63104929652061 88-89 22.574977003585733 25.875594308802004 27.57200042885858 23.97742825875369 90-91 22.150625950000627 25.83612128970524 27.830796617683852 24.182456142610288 92-93 22.517232419776562 26.115418657086682 27.52504170263294 23.84230722050382 94-95 21.402884299300787 26.10482597904195 28.142403262931197 24.349886458726065 96-97 21.74094044307015 25.964368356032463 27.706888041832382 24.587803159064997 98-99 21.01832823071046 26.70172660652129 27.633664947476095 24.64628021529216 100 21.82200434234762 25.94715403542245 26.75349106642039 25.47735055580954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 776.0 1 503.5 2 328.5 3 431.5 4 319.5 5 254.0 6 493.5 7 1118.5 8 1912.0 9 2205.0 10 1759.0 11 1329.5 12 1278.5 13 1400.0 14 1641.0 15 2306.5 16 2990.5 17 3766.0 18 5080.5 19 6448.0 20 8141.5 21 10157.0 22 12511.5 23 14904.0 24 17182.0 25 20074.5 26 24105.0 27 28083.0 28 33237.5 29 44937.5 30 61416.5 31 75653.5 32 95294.5 33 113897.5 34 124583.5 35 140383.0 36 156159.5 37 181582.0 38 193507.0 39 181847.0 40 170476.0 41 159197.0 42 157187.5 43 166476.0 44 182324.5 45 217036.0 46 250372.0 47 272209.5 48 288032.0 49 283969.5 50 271319.5 51 275959.0 52 277828.5 53 264380.0 54 254697.0 55 220829.5 56 189980.5 57 157675.5 58 122744.5 59 120482.5 60 101288.5 61 68188.5 62 50176.5 63 41315.5 64 29876.5 65 14212.5 66 7714.0 67 5258.0 68 3829.5 69 2970.0 70 2365.5 71 1856.5 72 1509.5 73 1392.0 74 1105.5 75 794.0 76 544.0 77 332.0 78 171.5 79 91.0 80 39.0 81 9.5 82 5.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.008145737219805153 3 0.0 4 0.09343450360424724 5 0.0045558174569265975 6 3.21965898016014E-5 7 4.829488470240209E-5 8 0.0 9 1.60982949008007E-5 10-11 0.0 12-13 2.4147442351201047E-4 14-15 0.009892402216542029 16-17 0.0 18-19 6.43931796032028E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.21965898016014E-5 60-61 0.0 62-63 0.0 64-65 8.854062195440383E-5 66-67 1.5293380155760662E-4 68-69 0.0 70-71 4.990471419248216E-4 72-73 3.783099301688164E-4 74-75 4.2660481487121843E-4 76-77 1.0463891685520453E-4 78-79 3.21965898016014E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.06658254770971168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6211838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.084826436634394 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25686346006142 41.51114583520051 2 16.652483426676707 19.678181860082482 3 6.253574187043277 11.084740363502075 4 2.8335372646956998 6.6967622994512475 5 1.4572102171537273 4.304950638110916 6 0.8368103438845408 2.9665676357279067 7 0.47817524149752455 1.977703080512388 8 0.324044878213943 1.5316908309560922 9 0.20977845887828517 1.1155251449671313 >10 0.6739062801197482 6.007597319067604 >50 0.01519203160484813 0.6063466966442308 >100 0.007050325782731432 0.7837842090095332 >500 7.728100155457557E-4 0.3333531771109425 >1k 4.722727209297129E-4 0.4582876413266325 >5k 4.2933883720882046E-5 0.21382190461320325 >10k+ 8.586776744176409E-5 0.7295413637170617 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15222 0.24504824497998823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13537 0.21792261807213903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8429 0.13569252771884907 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6098294900800696E-5 2 0.0 0.0 0.0 0.0 1.6098294900800696E-5 3 0.0 0.0 0.0 0.0 1.6098294900800696E-5 4 0.0 0.0 0.0 0.0 1.6098294900800696E-5 5 0.0 0.0 0.0 0.0 1.6098294900800696E-5 6 0.0 0.0 0.0 0.0 3.219658980160139E-5 7 0.0 0.0 0.0 0.0 3.219658980160139E-5 8 0.0 0.0 0.0 0.0 3.219658980160139E-5 9 0.0 0.0 0.0 0.0 3.219658980160139E-5 10-11 0.0 0.0 0.0 0.0 3.219658980160139E-5 12-13 0.0 0.0 0.0 0.0 1.2073721175600522E-4 14-15 0.0 0.0 0.0 0.0 1.3683550665680592E-4 16-17 0.0 0.0 0.0 0.0 1.6098294900800697E-4 18-19 0.0 0.0 0.0 0.0 1.7708124390880767E-4 20-21 0.0 0.0 0.0 2.4147442351201043E-5 2.0927783371040907E-4 22-23 0.0 0.0 0.0 8.049147450400348E-5 2.2537612861120977E-4 24-25 0.0 0.0 0.0 1.2878635920640557E-4 2.2537612861120977E-4 26-27 0.0 0.0 0.0 4.266048148712185E-4 2.495235709624108E-4 28-29 0.0 0.0 0.0 0.0027125626907849175 2.5757271841281114E-4 30-31 0.0 0.0 0.0 0.010351203621214848 2.5757271841281114E-4 32-33 0.0 0.0 0.0 0.02495235709624108 2.5757271841281114E-4 34-35 0.0 0.0 0.0 0.04786023074008047 2.8976930821441254E-4 36-37 0.0 0.0 0.0 0.08748618363840138 2.8976930821441254E-4 38-39 0.0 0.0 0.0 0.14395900215041024 2.8976930821441254E-4 40-41 0.0 0.0 0.0 0.20492324493974245 2.8976930821441254E-4 42-43 0.0 0.0 0.0 0.2699040123068245 2.8976930821441254E-4 44-45 0.0 0.0 0.0 0.3497676533096967 2.8976930821441254E-4 46-47 0.0 0.0 0.0 0.453014067655982 2.8976930821441254E-4 48-49 0.0 0.0 0.0 0.570813340592591 3.0586760311521324E-4 50-51 0.0 0.0 0.0 0.6955348803365445 3.2196589801601394E-4 52-53 0.0 0.0 0.0 0.818107297711241 3.3806419291681464E-4 54-55 0.0 0.0 0.0 0.953840071167342 3.5416248781761534E-4 56-57 0.0 0.0 0.0 1.1220028597011062 3.8635907761921674E-4 58-59 0.0 0.0 0.0 1.3230061698325037 4.0245737252001744E-4 60-61 0.0 0.0 0.0 1.5331372131726553 4.0245737252001744E-4 62-63 0.0 0.0 0.0 1.7546175544178713 4.6685055212322024E-4 64-65 0.0 0.0 0.0 1.9849358595636266 4.748996995736206E-4 66-67 0.0 0.0 0.0 2.247933703358008 4.8294884702402093E-4 68-69 0.0 0.0 0.0 2.5447621138864216 4.8294884702402093E-4 70-71 0.0 0.0 0.0 2.8758879417009906 4.909979944744213E-4 72-73 0.0 0.0 0.0 3.221123924996112 5.392928791768234E-4 74-75 0.0 0.0 0.0 3.5600654749850205 5.956369113296258E-4 76-77 0.0 0.0 0.0 3.9518899237230594 5.956369113296258E-4 78-79 0.0 0.0 0.0 4.376257075603066 6.117352062304265E-4 80-81 0.0 0.0 0.0 4.8440332796830825 6.278335011312272E-4 82-83 0.0 0.0 0.0 5.329887868936698 6.600300909328286E-4 84-85 0.0 0.0 0.0 5.8066308232764605 6.600300909328286E-4 86-87 0.0 0.0 0.0 6.316455773637368 6.600300909328286E-4 88 0.0 0.0 0.0 6.728201862315148 6.600300909328286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12100 0.0 45.762375 1 GTATCAA 20825 0.0 39.466206 1 GGGCACC 6720 0.0 39.403122 94 GTGGTAT 2895 0.0 35.263763 1 GTACTAG 6695 0.0 33.096935 1 CAACGCA 24055 0.0 32.70543 5 ATCAACG 24280 0.0 32.471382 3 CTGTGCG 9105 0.0 30.56321 9 TATCAAC 27180 0.0 30.18662 2 TAGGCAT 8215 0.0 29.576908 5 TAGTACT 9705 0.0 29.324818 4 AACGCAG 27995 0.0 29.092958 6 ACTGTGC 10660 0.0 28.04059 8 GTACTGT 10485 0.0 27.566174 6 TCAACGC 29370 0.0 27.132195 4 CTAGTAC 10925 0.0 26.997375 3 CTAGGCA 9260 0.0 26.771648 4 ACGCAGA 29405 0.0 26.723194 7 CAGTACG 1115 0.0 26.55968 9 CGCAGAG 29760 0.0 26.42106 8 >>END_MODULE