FastQCFastQC Report
Mon 16 Jan 2017
ERR1146669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704981
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC97660.1711837427679426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC62800.044.3259894
GTACTAG71600.037.6252441
TAGGCAT85950.034.6108935
GCATCGT74950.033.3567358
CTGTGCG90200.032.7187469
TATTCTC33450.032.594695
TAGTACT91800.032.354674
CAACGCA119500.031.933255
GGTATCA87850.031.7894061
GTATCAA133200.031.6963921
CTAGGCA98750.031.3149264
ACTGTGC97200.030.990988
TACCCCG3650.030.9010585
GTGGTAT19550.030.7822911
GTACTGT99450.029.8172876
CTAACGC39600.029.7882443
TACTGTG108100.029.6049947
ATCAACG128700.029.5052453
TCTAACG40450.029.2784732
CGCCTAC40400.028.8478787
Produced by FastQC (version 0.11.5)