##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146669_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5704981 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.406753852466817 31.0 31.0 34.0 28.0 34.0 2 31.435523273434214 31.0 31.0 34.0 28.0 34.0 3 31.58710607449876 33.0 31.0 34.0 28.0 34.0 4 35.01973205519878 37.0 35.0 37.0 32.0 37.0 5 35.19404832373675 37.0 35.0 37.0 32.0 37.0 6 35.24627917253362 37.0 35.0 37.0 32.0 37.0 7 35.17751557805364 37.0 35.0 37.0 32.0 37.0 8 35.17319005970397 37.0 35.0 37.0 32.0 37.0 9 36.64866140658488 39.0 37.0 39.0 32.0 39.0 10-11 36.468268868905966 39.0 37.0 39.0 32.0 39.0 12-13 36.383313809458784 39.0 37.0 39.0 31.5 39.0 14-15 37.54816738215254 40.0 37.0 41.0 31.0 41.0 16-17 37.58321482578119 40.0 37.0 41.0 31.0 41.0 18-19 37.55863980966808 40.0 37.0 41.0 31.0 41.0 20-21 37.6382583044536 40.0 37.0 41.0 31.0 41.0 22-23 37.58080377480661 40.0 37.0 41.0 31.0 41.0 24-25 37.539991631873974 40.0 37.0 41.0 31.0 41.0 26-27 37.5811480353747 40.0 37.0 41.0 31.0 41.0 28-29 37.587976804830724 40.0 37.0 41.0 31.0 41.0 30-31 37.463008816330856 40.0 37.0 41.0 31.0 41.0 32-33 37.45916480703441 40.0 36.0 41.0 31.0 41.0 34-35 37.401637533937446 40.0 36.0 41.0 30.5 41.0 36-37 37.284648625473075 40.0 36.0 41.0 30.0 41.0 38-39 37.17191652347309 40.0 36.0 41.0 30.0 41.0 40-41 37.07576239079499 40.0 36.0 41.0 30.0 41.0 42-43 36.906073306817326 39.5 35.0 41.0 30.0 41.0 44-45 36.72784405416951 39.0 35.0 41.0 30.0 41.0 46-47 36.71092015906802 39.0 35.0 41.0 30.0 41.0 48-49 36.56070581129017 39.0 35.0 41.0 29.5 41.0 50-51 36.360629158975286 39.0 35.0 41.0 29.0 41.0 52-53 36.12934293733844 38.5 35.0 40.5 29.0 41.0 54-55 35.912380689786694 38.0 34.5 40.0 28.5 41.0 56-57 35.655067475246625 37.5 34.0 40.0 28.0 41.0 58-59 35.331621788048025 37.0 34.0 40.0 28.0 41.0 60-61 35.079266872229724 36.5 34.0 40.0 28.0 41.0 62-63 34.766010701876134 36.0 33.5 39.5 27.0 41.0 64-65 34.60728046245903 35.5 33.0 39.0 27.0 41.0 66-67 34.36048472378786 35.0 33.0 39.0 27.0 41.0 68-69 34.2420739876259 35.0 33.0 38.5 27.0 41.0 70-71 34.181164319390376 35.0 33.0 38.0 27.0 41.0 72-73 33.97669238512801 35.0 33.0 37.0 27.0 40.0 74-75 33.734361954930264 35.0 33.0 37.0 27.0 39.5 76-77 33.488479365663096 35.0 33.0 36.0 26.5 39.0 78-79 33.251088829217835 35.0 33.0 36.0 26.0 39.0 80-81 33.08247845172491 35.0 33.0 36.0 26.0 38.5 82-83 32.82544735907096 35.0 33.0 35.0 26.0 37.0 84-85 32.656337856339924 35.0 33.0 35.0 26.0 37.0 86-87 32.48627821898093 35.0 33.0 35.0 25.0 36.5 88-89 32.36756423903954 35.0 33.0 35.0 25.0 36.0 90-91 32.21676855716084 35.0 32.0 35.0 25.0 36.0 92-93 32.05987530896246 35.0 32.0 35.0 25.0 36.0 94-95 32.00314277996719 35.0 32.0 35.0 25.0 35.5 96-97 31.891464669207487 35.0 32.0 35.0 25.0 35.0 98-99 31.80334614611337 35.0 32.0 35.0 24.0 35.0 100 30.85846788972654 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43298676651389556 1101 2 0.435593171187282 1101 3 0.5050008369065964 1101 4 0.6118607982200217 1101 5 0.48143494639639073 1101 6 0.46109118678332806 1101 7 0.533070525178239 1101 8 0.4754089131922328 1101 9 0.7122762258881039 1101 10-11 0.7426285064815445 1101 12-13 0.6419707634033784 1101 14-15 0.7919316540214183 1101 16-17 0.7634838836265061 1101 18-19 0.764162002639388 1101 20-21 0.7638427594870549 1101 22-23 0.7503006041981735 1101 24-25 0.8340196471776125 1101 26-27 0.7968419588302567 1101 28-29 0.7926759680473836 1101 30-31 0.8013568488389851 1101 32-33 0.7308982181420802 1101 34-35 0.7998188777048085 1101 36-37 0.8419517661757894 1101 38-39 0.7923600282868648 1101 40-41 0.8102955132442489 1101 42-43 0.7633667963859807 1101 44-45 0.7591111380099846 1101 46-47 0.7289135281119385 1101 48-49 0.7292822852892087 1101 50-51 0.6744556373435273 1101 52-53 0.6495251705989133 1101 54-55 0.6498959627196967 1101 56-57 0.580255935572815 1101 58-59 0.623592671302994 1101 60-61 0.6820776035022718 1101 62-63 0.6755501494731107 1101 64-65 0.6723231519940214 1101 66-67 0.6817691233222902 1101 68-69 0.6787063913056244 1101 70-71 0.6385904552404043 1101 72-73 0.6813677097222453 1101 74-75 0.5814700667053003 1101 76-77 0.6394393038405894 1101 78-79 0.6463777753713771 1101 80-81 0.5298384108941363 1101 82-83 0.5567868537615936 1101 84-85 0.5780016549941891 1101 86-87 0.5736539601582891 1101 88-89 0.6255299553854456 1101 90-91 0.6301914597512308 1101 92-93 0.6055788741605141 1101 94-95 0.5821667860650734 1101 96-97 0.5798598122243277 1101 98-99 0.6096563737826273 1101 100 0.7223191144939136 1102 1 0.31399485648810455 1102 2 0.4142783660826552 1102 3 0.44468219607933435 1102 4 0.46651303871720984 1102 5 0.3340733433354188 1102 6 0.38366514572231125 1102 7 0.32888223352539114 1102 8 0.41983781142668164 1102 9 0.4662666377705236 1102 10-11 0.5968384082580442 1102 12-13 0.5109483162218567 1102 14-15 0.6685567085669177 1102 16-17 0.6182763075392472 1102 18-19 0.6433754714239086 1102 20-21 0.5871153169485765 1102 22-23 0.6472692270120461 1102 24-25 0.6993845937141145 1102 26-27 0.65456765234579 1102 28-29 0.6607345406972343 1102 30-31 0.7067521416962919 1102 32-33 0.6297739715239032 1102 34-35 0.6895756642348374 1102 36-37 0.6991228048052918 1102 38-39 0.7050062310284702 1102 40-41 0.67985020796187 1102 42-43 0.700916675048255 1102 44-45 0.7026426067493148 1102 46-47 0.5930749512768045 1102 48-49 0.5602865853173853 1102 50-51 0.5302552059733543 1102 52-53 0.5798758758558478 1102 54-55 0.5426983350178318 1102 56-57 0.5257818374016168 1102 58-59 0.5481796414982725 1102 60-61 0.5135854758889948 1102 62-63 0.4973216356432246 1102 64-65 0.5297694447431311 1102 66-67 0.5688057095291512 1102 68-69 0.4517126418926978 1102 70-71 0.4824344979208419 1102 72-73 0.5344440312095387 1102 74-75 0.4717044310228502 1102 76-77 0.4782085641976863 1102 78-79 0.4959022783385336 1102 80-81 0.46985184365187393 1102 82-83 0.4611225966088597 1102 84-85 0.44806093272261904 1102 86-87 0.4242524743805447 1102 88-89 0.5122102420777708 1102 90-91 0.4415275896188575 1102 92-93 0.4121226247030023 1102 94-95 0.502356854087914 1102 96-97 0.5387528685675989 1102 98-99 0.4849195595250535 1102 100 0.45809519437586843 1103 1 0.3501281898526756 1103 2 0.3558516977228372 1103 3 0.36952152753700673 1103 4 0.4307502964345815 1103 5 0.33928949920883156 1103 6 0.3508996400848403 1103 7 0.3419950193804482 1103 8 0.31033505832211006 1103 9 0.48295064593652626 1103 10-11 0.5672308927388414 1103 12-13 0.46261690154325663 1103 14-15 0.5888075665728465 1103 16-17 0.5764383198622909 1103 18-19 0.6395586966452385 1103 20-21 0.5392270945287976 1103 22-23 0.6107836212569993 1103 24-25 0.5931475756772713 1103 26-27 0.5628076135564442 1103 28-29 0.6174381011011221 1103 30-31 0.5479740853976054 1103 32-33 0.5497601529925191 1103 34-35 0.5865018639524493 1103 36-37 0.6084056574674932 1103 38-39 0.6376329831944645 1103 40-41 0.5997486032152111 1103 42-43 0.5673788322925049 1103 44-45 0.5324550368205294 1103 46-47 0.47076049194559033 1103 48-49 0.5174607061929848 1103 50-51 0.45130804486973375 1103 52-53 0.4811207473544101 1103 54-55 0.47971486653131734 1103 56-57 0.48138790171730506 1103 58-59 0.45127675088782837 1103 60-61 0.5107783084805817 1103 62-63 0.4800536697659581 1103 64-65 0.4207596855517721 1103 66-67 0.47195147840050566 1103 68-69 0.45132421534616896 1103 70-71 0.44290479715718334 1103 72-73 0.39145460761076123 1103 74-75 0.41646398494528825 1103 76-77 0.37682623368995394 1103 78-79 0.3876881302288524 1103 80-81 0.3472760757282316 1103 82-83 0.398775054687313 1103 84-85 0.37450424060444476 1103 86-87 0.4116551305234424 1103 88-89 0.4752502978954567 1103 90-91 0.48994457938370317 1103 92-93 0.4669498971608377 1103 94-95 0.41628597706453263 1103 96-97 0.38205229245573946 1103 98-99 0.40456758468518217 1103 100 0.5355020478566779 1104 1 0.2528086264172771 1104 2 0.2981183322178751 1104 3 0.24257128779226278 1104 4 0.4220208223746056 1104 5 0.291922063948725 1104 6 0.3391658647221405 1104 7 0.3031389631814605 1104 8 0.3675637038524613 1104 9 0.3513865318462379 1104 10-11 0.4832283454509536 1104 12-13 0.37058640349999905 1104 14-15 0.547451984134149 1104 16-17 0.4859552000516203 1104 18-19 0.4666076548133091 1104 20-21 0.45974402760299427 1104 22-23 0.5225580518632285 1104 24-25 0.5322256656961315 1104 26-27 0.46962527203476157 1104 28-29 0.390564957580402 1104 30-31 0.3955974889356071 1104 32-33 0.4558101545349942 1104 34-35 0.5419636039527944 1104 36-37 0.4870554296218401 1104 38-39 0.4734313849696363 1104 40-41 0.5118831742748426 1104 42-43 0.45713189300433754 1104 44-45 0.41138821127890424 1104 46-47 0.39906499751612756 1104 48-49 0.33933914921835395 1104 50-51 0.260076108358021 1104 52-53 0.38562370119473144 1104 54-55 0.4305068725425656 1104 56-57 0.4254552109351337 1104 58-59 0.3969597204173283 1104 60-61 0.39859330398537196 1104 62-63 0.436122814529476 1104 64-65 0.4383960183386364 1104 66-67 0.386280496108121 1104 68-69 0.34666381884186137 1104 70-71 0.3528945011341236 1104 72-73 0.3374415825080703 1104 74-75 0.32053256670529606 1104 76-77 0.26865629256844414 1104 78-79 0.2916902753713799 1104 80-81 0.3470787490583831 1104 82-83 0.34516497372939625 1104 84-85 0.3766228305175403 1104 86-87 0.3577622532339717 1104 88-89 0.3085085181873808 1104 90-91 0.365967929155417 1104 92-93 0.37574795628612634 1104 94-95 0.416272563037694 1104 96-97 0.37753493299727836 1104 98-99 0.3732710074380208 1104 100 0.43113050740856806 1105 1 0.15117098130948037 1105 2 0.24693501270433416 1105 3 0.21647606943958309 1105 4 0.2585646656581915 1105 5 0.1841285661598917 1105 6 0.21890864388189613 1105 7 0.22083347672485587 1105 8 0.1934827198834128 1105 9 0.37853291797117095 1105 10-11 0.38841767657311976 1105 12-13 0.229305827214759 1105 14-15 0.42211469488594133 1105 16-17 0.366487434711658 1105 18-19 0.4270178434534344 1105 20-21 0.3392592120971969 1105 22-23 0.4107218336487506 1105 24-25 0.363748606547432 1105 26-27 0.36996040194078716 1105 28-29 0.40638516211328835 1105 30-31 0.47358645037839153 1105 32-33 0.3806840489517995 1105 34-35 0.40732257299646335 1105 36-37 0.35228483813483535 1105 38-39 0.41195059447765203 1105 40-41 0.40789616487739266 1105 42-43 0.36648227650350407 1105 44-45 0.3956962716713832 1105 46-47 0.43055900318223905 1105 48-49 0.31427005026866084 1105 50-51 0.40344390479251757 1105 52-53 0.39930874818200834 1105 54-55 0.46784837682669433 1105 56-57 0.43251755546250337 1105 58-59 0.4160288711912372 1105 60-61 0.4172487247979717 1105 62-63 0.3583014180396944 1105 64-65 0.38653380164432605 1105 66-67 0.37035146072945935 1105 68-69 0.3872551331038778 1105 70-71 0.35023462343926326 1105 72-73 0.28532888212112084 1105 74-75 0.32337123793249134 1105 76-77 0.3417197945032129 1105 78-79 0.3730698359020579 1105 80-81 0.3764591906006771 1105 82-83 0.35385027983774364 1105 84-85 0.29744666274528697 1105 86-87 0.24921169007753008 1105 88-89 0.3006649236600083 1105 90-91 0.28617073457277087 1105 92-93 0.289280553715642 1105 94-95 0.274556231500938 1105 96-97 0.22393703706029555 1105 98-99 0.25582611385039655 1105 100 0.2758789872316747 1106 1 0.05701536477871727 1106 2 0.16845652679391776 1106 3 0.12485854591451329 1106 4 0.27680869900905947 1106 5 0.12070393706687099 1106 6 0.22106878989005452 1106 7 0.186952630458741 1106 8 0.21220442722221833 1106 9 0.33019525102590563 1106 10-11 0.3417451268713734 1106 12-13 0.2465047954209183 1106 14-15 0.3806044355480367 1106 16-17 0.2997816126282089 1106 18-19 0.32280870023052444 1106 20-21 0.3062815765575735 1106 22-23 0.2881264645766848 1106 24-25 0.34460962885913204 1106 26-27 0.3232282043089967 1106 28-29 0.3535073583026147 1106 30-31 0.44435891825150264 1106 32-33 0.3077894922794968 1106 34-35 0.3004709760918445 1106 36-37 0.3231534466980506 1106 38-39 0.30490112129920277 1106 40-41 0.31902741790022304 1106 42-43 0.28319645778230296 1106 44-45 0.3375678259063193 1106 46-47 0.31463962825118585 1106 48-49 0.27178718761349074 1106 50-51 0.2731447118890671 1106 52-53 0.19853629489274738 1106 54-55 0.292929032123979 1106 56-57 0.26313405308029303 1106 58-59 0.30172940358116307 1106 60-61 0.24410961691569355 1106 62-63 0.2548744595087342 1106 64-65 0.26469897351097416 1106 66-67 0.29664620131730857 1106 68-69 0.34348779158521126 1106 70-71 0.32001843913551653 1106 72-73 0.2138510665373019 1106 74-75 0.2460648490942745 1106 76-77 0.20668597589019555 1106 78-79 0.22197075749505757 1106 80-81 0.24845855766176328 1106 82-83 0.28305723548375283 1106 84-85 0.25039751836627744 1106 86-87 0.2655980132407052 1106 88-89 0.2729713403401206 1106 90-91 0.23079580510555786 1106 92-93 0.30206420289088953 1106 94-95 0.2900799201745343 1106 96-97 0.2852543600707378 1106 98-99 0.27984371946926245 1106 100 0.2531453964448467 1107 1 0.13293118783580482 1107 2 0.1117489595472847 1107 3 0.11657050735971808 1107 4 0.24421539977362983 1107 5 0.19300202261390353 1107 6 0.2178393293670311 1107 7 0.14077278250159253 1107 8 0.14303874622497403 1107 9 0.19227957583582622 1107 10-11 0.26845276844971266 1107 12-13 0.17027951353450277 1107 14-15 0.27606464638196115 1107 16-17 0.19630697029315058 1107 18-19 0.14754894077589853 1107 20-21 0.1445944618131776 1107 22-23 0.27176467516292746 1107 24-25 0.28936766388449087 1107 26-27 0.18933342323114744 1107 28-29 0.2521340999044881 1107 30-31 0.312794960528727 1107 32-33 0.2964157653261452 1107 34-35 0.288117676686511 1107 36-37 0.30665717662333947 1107 38-39 0.3012970289670065 1107 40-41 0.26765152470851916 1107 42-43 0.3100397879118475 1107 44-45 0.2766759741614493 1107 46-47 0.26668270825453533 1107 48-49 0.23495033761606976 1107 50-51 0.23871375363521707 1107 52-53 0.22502485703478214 1107 54-55 0.22865656101810572 1107 56-57 0.2514732862717395 1107 58-59 0.2506978653010705 1107 60-61 0.2387246695909937 1107 62-63 0.24513094637716648 1107 64-65 0.28179395894792947 1107 66-67 0.214226831642236 1107 68-69 0.19184247249843622 1107 70-71 0.30431212928936446 1107 72-73 0.21422557888478622 1107 74-75 0.2321225169608283 1107 76-77 0.18413582621136015 1107 78-79 0.18803633505176265 1107 80-81 0.12043513866069588 1107 82-83 0.11430296058094314 1107 84-85 0.12581823915288481 1107 86-87 0.1905025334978916 1107 88-89 0.2080883736235961 1107 90-91 0.16981778831004135 1107 92-93 0.1531978875388802 1107 94-95 0.13389057103226776 1107 96-97 0.17542792726401757 1107 98-99 0.11625134367256962 1107 100 0.15289325267192666 1108 1 0.006774996720672277 1108 2 0.04301339115565739 1108 3 -0.0012322920081508926 1108 4 0.11458976450842329 1108 5 0.06680569531970093 1108 6 0.04546211915921816 1108 7 0.062126137516735014 1108 8 0.12295872930286578 1108 9 0.08004000448705284 1108 10-11 0.21882542535183092 1108 12-13 0.08779901903144349 1108 14-15 0.167905863646574 1108 16-17 0.19353663716233171 1108 18-19 0.16109479566120655 1108 20-21 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2215 36-37 -0.9151364356863709 2215 38-39 -0.770490223706517 2215 40-41 -0.7923145301337087 2215 42-43 -0.8055026706250317 2215 44-45 -0.758275099306772 2215 46-47 -1.0054959485943442 2215 48-49 -1.0250445413493736 2215 50-51 -0.9317990117880797 2215 52-53 -0.8913224653437766 2215 54-55 -0.8144690181405068 2215 56-57 -0.7869294488111152 2215 58-59 -0.9000344244628451 2215 60-61 -0.8005464499554265 2215 62-63 -0.786139856878961 2215 64-65 -0.8223826885689647 2215 66-67 -0.7765092562307174 2215 68-69 -0.7812887715080024 2215 70-71 -0.9368215936371129 2215 72-73 -0.8327870154670691 2215 74-75 -0.9257122670125071 2215 76-77 -0.8777677590783597 2215 78-79 -0.7922095468124013 2215 80-81 -0.7632081598228098 2215 82-83 -0.7943905257579829 2215 84-85 -0.7731240617034061 2215 86-87 -0.7898824827650017 2215 88-89 -0.7991598064954388 2215 90-91 -0.8506749085352112 2215 92-93 -0.7744010248749689 2215 94-95 -0.7288493051540215 2215 96-97 -0.9825777640423219 2215 98-99 -0.850050751821243 2215 100 -0.4530442490953419 2216 1 -0.474934522171921 2216 2 -0.5832862759106447 2216 3 -0.6037248017626098 2216 4 -0.46922502212276385 2216 5 -0.4301321464092993 2216 6 -0.3888636896536113 2216 7 -0.5200054636210538 2216 8 -0.5970231785419955 2216 9 -0.6672283281760372 2216 10-11 -0.9974092175362657 2216 12-13 -0.6003783656938708 2216 14-15 -0.7180347747667639 2216 16-17 -0.702441282449783 2216 18-19 -0.9859933590466383 2216 20-21 -1.0846033000573527 2216 22-23 -0.9309935935228495 2216 24-25 -1.054516954324157 2216 26-27 -1.3782652590701687 2216 28-29 -1.195860417724198 2216 30-31 -1.3621762470473229 2216 32-33 -1.6063704471928162 2216 34-35 -1.2167190879308123 2216 36-37 -1.2896902605085998 2216 38-39 -1.205083029731611 2216 40-41 -1.0952970759925407 2216 42-43 -1.0278164009654347 2216 44-45 -0.9867059256721262 2216 46-47 -1.0835843145862398 2216 48-49 -1.1497575320304705 2216 50-51 -1.2249197878247102 2216 52-53 -1.1564803441836347 2216 54-55 -1.236710932304561 2216 56-57 -1.1515685808679805 2216 58-59 -1.0463079346111712 2216 60-61 -1.140106416500224 2216 62-63 -1.2390905770773841 2216 64-65 -1.15105734355258 2216 66-67 -1.130762732834576 2216 68-69 -1.1004824775922657 2216 70-71 -1.0262911340582264 2216 72-73 -0.9607918464776262 2216 74-75 -1.0386516626453712 2216 76-77 -1.0393184822212334 2216 78-79 -0.9412206335466209 2216 80-81 -0.9983235284051375 2216 82-83 -1.031716183719361 2216 84-85 -1.0708297705652363 2216 86-87 -1.0440168945503103 2216 88-89 -1.0349101269947205 2216 90-91 -1.1500336331206142 2216 92-93 -1.0989067855351848 2216 94-95 -1.0930133486368518 2216 96-97 -1.1311579236730296 2216 98-99 -1.1289578546296184 2216 100 -0.7525798878259025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 1.0 2 6.0 3 23.0 4 58.0 5 35.0 6 57.0 7 207.0 8 705.0 9 1109.0 10 1595.0 11 2715.0 12 4576.0 13 6900.0 14 9429.0 15 12947.0 16 16048.0 17 18857.0 18 21682.0 19 24586.0 20 28153.0 21 32564.0 22 38055.0 23 44262.0 24 51934.0 25 58797.0 26 66178.0 27 73061.0 28 84173.0 29 102692.0 30 120376.0 31 139094.0 32 172067.0 33 223572.0 34 329180.0 35 476508.0 36 756741.0 37 1312351.0 38 1040322.0 39 432809.0 40 556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31649495511635 21.564954883075895 12.34383328372667 23.774716878081087 2 15.456640439566296 19.77440042408387 41.02247125618719 23.74648788016265 3 18.144790407258192 27.480317072490916 29.099985397654475 25.274907122596417 4 11.819826945599829 16.87912364542132 41.10575797942974 30.19529142954911 5 12.732728121457464 39.0950815097307 35.30874728013127 12.86344308868056 6 28.867680049305385 39.41299812405366 18.402942476704926 13.316379349936035 7 26.367155010288467 32.07984461161757 24.437788993416397 17.11521138467757 8 26.22374122750203 34.98225999635752 19.93660540766425 18.857393368476203 9 26.327792887447337 14.820026657523739 21.88930116200721 36.962879293021714 10-11 25.716503834646314 25.869327018826954 27.609321995127395 20.804847151399336 12-13 25.768647842902574 23.9742035430689 27.600822405799015 22.656326208229512 14-15 23.290947513520656 25.07851920096393 24.996362823038282 26.63417046247713 16-17 21.888702179833334 27.509237803737086 26.46634605061267 24.135713965816905 18-19 21.200577433788677 28.59516382111402 28.123218361630226 22.08104038346708 20-21 23.218142501272364 27.579129224234254 26.834141275664557 22.368586998828818 22-23 23.92740106978118 27.172065091355595 26.509398491298274 22.391135347564948 24-25 23.21904492963473 27.21818812375551 27.541961883882642 22.02080506272711 26-27 23.04398031996922 27.54670545293914 26.251367825244408 23.157946401847227 28-29 21.946665963158555 28.171608416750026 26.927380696432714 22.954344923658706 30-31 22.958140805157587 27.670969358603443 26.923758886227628 22.447130950011346 32-33 23.564307595651986 27.897712128517465 25.736115368134154 22.801864907696395 34-35 23.36617713566518 27.619171992609708 27.04520595009799 21.969444921627126 36-37 22.949081762911433 27.231056155839983 26.618436474387497 23.201425606861086 38-39 23.184579893875675 27.238190732790734 27.345130183727957 22.232099189605638 40-41 22.63176148590037 26.18464404956285 28.181204566006013 23.002389898530765 42-43 22.60337450773783 26.59455601671099 27.265243089220736 23.536826386330446 44-45 22.791539584202454 27.81540126822258 27.02528897572754 22.36777017184743 46-47 22.790607936083276 27.43066963398167 26.47218001929199 23.306542410643065 48-49 23.66515394033665 26.877408680423365 26.765613210817957 22.691824168422027 50-51 23.127279191733884 26.681198794476806 26.703341486507465 23.488180527281838 52-53 22.32360868924501 27.565964151871817 26.557010667159226 23.553416491723947 54-55 22.46762227875096 27.390864487326922 26.524367126362243 23.617146107559876 56-57 23.876703904170085 26.617711872815832 26.200109184506736 23.305475038507346 58-59 22.48241884565804 27.155430325192402 27.33848681367404 23.023664015475518 60-61 22.564661365065568 27.924901951468627 26.39183683630423 23.118599847161576 62-63 23.097181671825474 26.979773142169496 26.00323281217582 23.91981237382921 64-65 21.99253391001862 27.34620023808253 26.55579113705751 24.105474714841346 66-67 23.023588271701954 26.716395787327148 26.470838568173683 23.789177372797216 68-69 22.239852036937478 26.903849076554337 26.82450176320546 24.031797123302727 70-71 22.27723275617705 26.57484148814709 27.010570495417358 24.137355260258502 72-73 23.058393795267566 26.687801500731574 27.506490726725612 22.747313977275248 74-75 23.241330230771652 27.04842830918365 26.906956545291084 22.803284914753622 76-77 22.820931663941554 26.989712943781708 26.53857501455467 23.650780377722068 78-79 22.631222842100456 26.89503005343466 26.65023040250732 23.823516701957566 80-81 22.115196416793214 27.300126437763915 27.499245098619262 23.085432046823605 82-83 23.173986881496276 26.110714940572667 28.64689880857846 22.068399369352598 84-85 23.52335446575139 26.8070250973468 26.93661922449332 22.7330012124085 86-87 21.90018518052669 27.645838977700414 27.482334573625934 22.971641268146957 88-89 23.306407886942925 26.07161132134412 27.200041651203776 23.421939140509178 90-91 22.807210845105992 26.06831774441823 27.568945306833275 23.55552610364251 92-93 23.15366315299 26.47770494406931 27.353553028843514 23.015078874097178 94-95 21.715204478665825 26.559122217399565 28.1452666956026 23.58040660833201 96-97 22.235379644616366 26.434565645243595 27.5252010412264 23.804853668913633 98-99 21.422752024191695 27.330468604485542 27.42819953764065 23.81857983368211 100 22.54447061163929 26.412839604585393 26.424789700282815 24.617900083492504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1199.0 1 795.0 2 487.0 3 602.0 4 562.0 5 472.0 6 440.0 7 441.5 8 485.0 9 502.5 10 439.0 11 399.5 12 398.5 13 409.5 14 454.0 15 819.0 16 978.0 17 1008.0 18 1411.0 19 1701.5 20 2098.0 21 2632.5 22 3602.0 23 5002.0 24 6412.0 25 8089.0 26 10835.0 27 13583.5 28 18600.5 29 31372.0 30 49490.5 31 66291.5 32 88083.5 33 108808.0 34 123317.5 35 140389.5 36 154928.0 37 184286.5 38 194616.0 39 174351.0 40 157487.5 41 140207.5 42 135643.5 43 145568.0 44 160766.5 45 198657.5 46 236647.0 47 260986.5 48 277077.5 49 269460.5 50 252704.5 51 260540.0 52 267541.5 53 255933.5 54 242992.5 55 207635.0 56 180269.5 57 146377.0 58 110777.0 59 108180.5 60 89355.5 61 59176.5 62 43330.5 63 37306.5 64 27561.0 65 11758.0 66 5445.5 67 3681.5 68 2829.0 69 2082.5 70 1477.0 71 1103.0 72 883.5 73 797.0 74 656.0 75 531.0 76 402.0 77 247.5 78 166.5 79 102.0 80 66.5 81 49.0 82 43.0 83 42.5 84 36.5 85 28.5 86 17.5 87 14.0 88 14.0 89 11.5 90 14.0 91 13.0 92 7.5 93 8.0 94 9.0 95 8.5 96 10.0 97 8.0 98 4.5 99 8.0 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060683812969753975 2 0.007870315431374793 3 0.007326930624308828 4 0.009325184430938507 5 0.0035407655170104865 6 0.0022261248547541176 7 8.764271081709124E-4 8 0.0013672262887466234 9 4.382135540854562E-4 10-11 3.8562792759520143E-4 12-13 2.0157823487930988E-4 14-15 1.2269979514392774E-4 16-17 4.645063673305836E-4 18-19 0.001481161812808842 20-21 9.903626322331311E-4 22-23 0.0013321692044197869 24-25 0.0012532907646844048 26-27 0.003742343751889796 28-29 0.004163028763811834 30-31 0.005197212751453511 32-33 0.006231396739095187 34-35 0.0035232369748470676 36-37 0.008115715021662648 38-39 0.006152518299359805 40-41 0.006056111317461005 42-43 0.014557454266718855 44-45 0.02053468714444448 46-47 0.018562726151059927 48-49 0.02211225593915212 50-51 0.018711718759448978 52-53 0.025372564781547917 54-55 0.019237575024351528 56-57 0.015337474392990966 58-59 0.017598656332071923 60-61 0.016091201706017952 62-63 0.011560073556774334 64-65 0.011507487930284081 66-67 0.014224411965613908 68-69 0.00550396223931333 70-71 0.009079784840650652 72-73 0.008702921184137161 74-75 0.01161265918326459 76-77 0.01100792447862666 78-79 0.01269942879739652 80-81 0.010403189773988731 82-83 0.006432974973974497 84-85 0.011095567189443751 86-87 0.009596876834471491 88-89 0.008133243563826068 90-91 0.013453156110423506 92-93 0.008939556503343308 94-95 0.007449630419452756 96-97 0.009710812358533709 98-99 0.009526762665817819 100 0.2567931426940773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5704981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.85460114684261 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66110178832685 45.670845717954165 2 15.270267940020815 19.196132015508528 3 5.811371904222537 10.958143894676246 4 2.6463722294763237 6.653466838792599 5 1.3575088280460288 4.266283797007545 6 0.7530272161129791 2.839873519289913 7 0.4556371195641656 2.004722259253131 8 0.31126175694450164 1.5651386868009698 9 0.19100629534873445 1.0805062059612651 >10 0.5352630978815296 4.731544750226605 >50 0.0045989474279718116 0.1895044715408553 >100 0.0021932197872527757 0.26507121519864 >500 1.731808435673126E-4 0.05878014881757727 >1k 1.7318079733670327E-4 0.25422407821621085 >5k 4.3295199334175574E-5 0.26576240075567414 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9766 0.1711837427679426 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.5057084326836495E-5 14-15 0.0 0.0 0.0 0.0 5.2585626490254746E-5 16-17 0.0 0.0 0.0 0.0 7.011416865367299E-5 18-19 0.0 0.0 0.0 0.0 7.011416865367299E-5 20-21 0.0 0.0 0.0 0.0 1.1393552406221861E-4 22-23 0.0 0.0 0.0 7.011416865367299E-5 1.2269979514392774E-4 24-25 0.0 0.0 0.0 1.3146406622563687E-4 1.2269979514392774E-4 26-27 0.0 0.0 0.0 5.170919938208384E-4 1.2269979514392774E-4 28-29 0.0 0.0 0.0 0.003093787691843321 1.2269979514392774E-4 30-31 0.0 0.0 0.0 0.0118142374181439 1.2269979514392774E-4 32-33 0.0 0.0 0.0 0.02709912618464461 1.4022833730734598E-4 34-35 0.0 0.0 0.0 0.050885357900403175 1.7528542163418247E-4 36-37 0.0 0.0 0.0 0.08852790219634386 1.7528542163418247E-4 38-39 0.0 0.0 0.0 0.1479058387749232 1.7528542163418247E-4 40-41 0.0 0.0 0.0 0.21121016879810817 1.7528542163418247E-4 42-43 0.0 0.0 0.0 0.2773628869228486 1.7528542163418247E-4 44-45 0.0 0.0 0.0 0.3589582506935606 1.840496927158916E-4 46-47 0.0 0.0 0.0 0.4607903163919389 1.9281396379760074E-4 48-49 0.0 0.0 0.0 0.5802298026934709 1.9281396379760074E-4 50-51 0.0 0.0 0.0 0.7094852726065171 1.9281396379760074E-4 52-53 0.0 0.0 0.0 0.8332192517380864 2.1034250596101899E-4 54-55 0.0 0.0 0.0 0.9679962124326094 2.1910677704272812E-4 56-57 0.0 0.0 0.0 1.1353937900932536 2.2787104812443723E-4 58-59 0.0 0.0 0.0 1.3413453962423363 2.2787104812443723E-4 60-61 0.0 0.0 0.0 1.5516002594925382 2.2787104812443723E-4 62-63 0.0 0.0 0.0 1.777972617262003 2.2787104812443723E-4 64-65 0.0 0.0 0.0 2.0150286214800714 2.2787104812443723E-4 66-67 0.0 0.0 0.0 2.2825054106227523 2.2787104812443723E-4 68-69 0.0 0.0 0.0 2.583943750207056 2.2787104812443723E-4 70-71 0.0 0.0 0.0 2.919440047214881 2.2787104812443723E-4 72-73 0.0 0.0 0.0 3.2672238522792627 2.3663531920614634E-4 74-75 0.0 0.0 0.0 3.609678980525965 2.6292813245127375E-4 76-77 0.0 0.0 0.0 4.010670675327402 2.8045667461469196E-4 78-79 0.0 0.0 0.0 4.43673694969361 3.067494878598193E-4 80-81 0.0 0.0 0.0 4.904538682950916 3.1551375894152845E-4 82-83 0.0 0.0 0.0 5.400263383874547 3.242780300232376E-4 84-85 0.0 0.0 0.0 5.882903028073187 3.330423011049467E-4 86-87 0.0 0.0 0.0 6.395884578756704 3.4180657218665586E-4 88 0.0 0.0 0.0 6.806928892488862 3.5057084326836494E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 6280 0.0 44.32598 94 GTACTAG 7160 0.0 37.625244 1 TAGGCAT 8595 0.0 34.610893 5 GCATCGT 7495 0.0 33.356735 8 CTGTGCG 9020 0.0 32.718746 9 TATTCTC 3345 0.0 32.59469 5 TAGTACT 9180 0.0 32.35467 4 CAACGCA 11950 0.0 31.93325 5 GGTATCA 8785 0.0 31.789406 1 GTATCAA 13320 0.0 31.696392 1 CTAGGCA 9875 0.0 31.314926 4 ACTGTGC 9720 0.0 30.99098 8 TACCCCG 365 0.0 30.901058 5 GTGGTAT 1955 0.0 30.782291 1 GTACTGT 9945 0.0 29.817287 6 CTAACGC 3960 0.0 29.788244 3 TACTGTG 10810 0.0 29.604994 7 ATCAACG 12870 0.0 29.505245 3 TCTAACG 4045 0.0 29.278473 2 CGCCTAC 4040 0.0 28.847878 7 >>END_MODULE