FastQCFastQC Report
Mon 16 Jan 2017
ERR1146669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704981
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178100.312183335930479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147640.25879139650070704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98430.17253344051452582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131100.046.101551
GGGCACC65450.043.0391594
GTGGTAT24800.042.292981
GTATCAA228900.039.86311
GTACTAG68850.034.771941
CTGTGCG91750.032.8933379
CAACGCA271300.032.6552475
ATCAACG276700.032.0044633
TAGTACT93750.031.6539174
TAGGCAT83700.031.5574765
ACTGTGC99200.031.3639058
TATTCTC37100.031.1639615
TATCAAC299700.030.4302942
GTACTGT101400.029.8483496
AACGCAG313400.029.3620286
TACTGTG113100.029.3369187
TACCCCG4850.029.0717375
CTAACGC33800.028.8020233
GAACCGT7200.028.720546
CTAGGCA96400.028.2956944
Produced by FastQC (version 0.11.5)