##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5704981
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12928544372014	33.0	31.0	34.0	30.0	34.0
2	32.202664478637175	33.0	31.0	34.0	30.0	34.0
3	32.362356158591936	34.0	31.0	34.0	30.0	34.0
4	35.97026861263868	37.0	35.0	37.0	35.0	37.0
5	35.87768583278367	37.0	35.0	37.0	35.0	37.0
6	35.9144614153842	37.0	35.0	37.0	35.0	37.0
7	35.84621684103768	37.0	35.0	37.0	35.0	37.0
8	35.85647191463039	37.0	35.0	37.0	35.0	37.0
9	37.55723112837711	39.0	37.0	39.0	35.0	39.0
10-11	37.49662593091897	39.0	37.0	39.0	34.5	39.0
12-13	37.45272429478731	39.0	37.0	39.0	34.0	39.0
14-15	38.657403854631596	40.0	38.0	41.0	34.0	41.0
16-17	38.66397188702294	40.0	38.0	41.0	34.0	41.0
18-19	38.73341322258567	40.0	38.0	41.0	34.0	41.0
20-21	38.73667843941987	40.0	38.0	41.0	34.0	41.0
22-23	38.691168910115564	40.0	38.0	41.0	34.0	41.0
24-25	38.60433706965895	40.0	38.0	41.0	34.0	41.0
26-27	38.44922621127047	40.0	38.0	41.0	34.0	41.0
28-29	38.3958974622352	40.0	38.0	41.0	34.0	41.0
30-31	38.24850985480933	40.0	37.5	41.0	34.0	41.0
32-33	38.085684597372016	40.0	37.0	41.0	33.0	41.0
34-35	37.97612244457957	40.0	37.0	41.0	33.0	41.0
36-37	37.95799801962531	40.0	37.0	41.0	33.0	41.0
38-39	37.848778637474865	40.0	37.0	41.0	33.0	41.0
40-41	37.716896690804056	40.0	36.5	41.0	33.0	41.0
42-43	37.56802204950375	40.0	36.0	41.0	32.5	41.0
44-45	37.32704455983289	40.0	36.0	41.0	31.5	41.0
46-47	37.0366812790437	39.5	35.5	41.0	31.0	41.0
48-49	36.71719791880113	39.0	35.0	41.0	30.5	41.0
50-51	36.48491081740676	39.0	35.0	41.0	30.0	41.0
52-53	36.28749035272861	39.0	35.0	41.0	30.0	41.0
54-55	36.08941677456945	38.0	35.0	41.0	30.0	41.0
56-57	35.8397984147537	38.0	35.0	40.0	29.0	41.0
58-59	35.53512930192055	37.0	35.0	40.0	29.0	41.0
60-61	35.34354128436186	37.0	35.0	40.0	29.0	41.0
62-63	35.11735139871631	36.0	34.0	40.0	29.0	41.0
64-65	34.863429606514025	36.0	34.0	39.0	28.5	41.0
66-67	34.61930109846115	35.0	34.0	39.0	28.5	41.0
68-69	34.38128531891692	35.0	34.0	39.0	28.0	41.0
70-71	34.18956399679509	35.0	34.0	38.0	28.0	41.0
72-73	33.97406319144621	35.0	34.0	37.0	28.0	40.0
74-75	33.73473049252925	35.0	34.0	37.0	28.0	39.5
76-77	33.514286813575715	35.0	33.5	36.0	27.0	39.0
78-79	33.31034704585344	35.0	33.0	36.0	27.0	39.0
80-81	33.202573680788774	35.0	33.0	36.0	27.0	38.5
82-83	33.05066318362849	35.0	33.0	35.0	27.0	37.0
84-85	32.93032369432957	35.0	33.0	35.0	27.0	37.0
86-87	32.795617461302676	35.0	33.0	35.0	27.0	36.5
88-89	32.697950089579614	35.0	33.0	35.0	27.0	36.0
90-91	32.63716802913103	35.0	33.0	35.0	27.0	36.0
92-93	32.57125448796411	35.0	33.0	35.0	27.0	36.0
94-95	32.53044672716702	35.0	33.0	35.0	27.0	35.5
96-97	32.44294363118826	35.0	33.0	35.0	27.0	35.0
98-99	32.37069615131058	35.0	33.0	35.0	27.0	35.0
100	31.152414705675618	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38533569720595295
1101	2	0.45176461307404736
1101	3	0.3739910478087012
1101	4	0.30270019139419446
1101	5	0.27512433441525275
1101	6	0.30843073562058265
1101	7	0.2853802211088521
1101	8	0.30143127014419235
1101	9	0.38453267520947776
1101	10-11	0.41779837528584096
1101	12-13	0.44024021028111093
1101	14-15	0.5527694026381482
1101	16-17	0.5759466504665482
1101	18-19	0.5572852811498734
1101	20-21	0.6141791367929414
1101	22-23	0.5759106713401181
1101	24-25	0.5499995080987645
1101	26-27	0.5619764213158476
1101	28-29	0.6121839920645371
1101	30-31	0.6581724233699688
1101	32-33	0.6409432699086608
1101	34-35	0.7025180692005719
1101	36-37	0.5522453554365256
1101	38-39	0.5642075832288143
1101	40-41	0.5950429342527244
1101	42-43	0.5693567235736268
1101	44-45	0.5351893377237502
1101	46-47	0.5699984735648798
1101	48-49	0.5427131561048242
1101	50-51	0.5538190196011001
1101	52-53	0.6182573358044152
1101	54-55	0.6169043350053798
1101	56-57	0.5534835817411548
1101	58-59	0.5740508664972737
1101	60-61	0.5371007722062444
1101	62-63	0.520807872089101
1101	64-65	0.4912229917065005
1101	66-67	0.4515587775500549
1101	68-69	0.5235198857022354
1101	70-71	0.4722089218469776
1101	72-73	0.51389203706659
1101	74-75	0.5043844714515515
1101	76-77	0.46779746366604513
1101	78-79	0.48350131426225573
1101	80-81	0.48614590853297557
1101	82-83	0.4251877652576752
1101	84-85	0.4258679485366912
1101	86-87	0.4275650414312935
1101	88-89	0.4274521637850839
1101	90-91	0.3789012532801834
1101	92-93	0.43354222745664117
1101	94-95	0.4530308658890263
1101	96-97	0.46403542797374087
1101	98-99	0.457198624022908
1101	100	0.7994045197136899
1102	1	0.32155750446624154
1102	2	0.3691170964844872
1102	3	0.30817104022285946
1102	4	0.236883394115587
1102	5	0.2290307432585834
1102	6	0.17390227624701993
1102	7	0.21936908134277644
1102	8	0.24042027305844016
1102	9	0.3522913219341959
1102	10-11	0.2637148347687912
1102	12-13	0.28001490616394875
1102	14-15	0.4296050444201782
1102	16-17	0.4018806120688936
1102	18-19	0.4307640116754996
1102	20-21	0.4363642324065893
1102	22-23	0.42619294057551116
1102	24-25	0.4149885960267028
1102	26-27	0.4654311429049258
1102	28-29	0.49260044916947976
1102	30-31	0.49162908600592203
1102	32-33	0.4602531787002633
1102	34-35	0.5036591550691512
1102	36-37	0.4551499997998789
1102	38-39	0.42787099262264405
1102	40-41	0.4224088266408472
1102	42-43	0.4344074167027472
1102	44-45	0.5025262600616713
1102	46-47	0.4154200849964269
1102	48-49	0.3997374272164578
1102	50-51	0.38834370389116657
1102	52-53	0.4040589197937763
1102	54-55	0.3616816853366984
1102	56-57	0.3369436330347284
1102	58-59	0.35733314702818575
1102	60-61	0.36685817999016734
1102	62-63	0.42388518936614616
1102	64-65	0.3371820917979349
1102	66-67	0.28499204655835086
1102	68-69	0.33823816340808577
1102	70-71	0.26777903971281347
1102	72-73	0.30297868682563944
1102	74-75	0.3255086497020798
1102	76-77	0.3023740181978738
1102	78-79	0.3369437891237652
1102	80-81	0.2797920565804546
1102	82-83	0.22203363840773704
1102	84-85	0.2680238452171153
1102	86-87	0.2750162358439354
1102	88-89	0.2616412817947875
1102	90-91	0.2438899399197183
1102	92-93	0.23574773824624629
1102	94-95	0.2362268396881646
1102	96-97	0.2682596572324769
1102	98-99	0.2749154624410153
1102	100	0.5127566333442992
1103	1	0.2643206871003443
1103	2	0.3575883868923526
1103	3	0.27466745678808735
1103	4	0.17900104422020036
1103	5	0.2049807175040499
1103	6	0.219087151819906
1103	7	0.22036511443087647
1103	8	0.18647220993132407
1103	9	0.28287048076331445
1103	10-11	0.28536396976925715
1103	12-13	0.30305202981954693
1103	14-15	0.4161070242446101
1103	16-17	0.3627226738462639
1103	18-19	0.35032977112128805
1103	20-21	0.3505960604110925
1103	22-23	0.3214671926193873
1103	24-25	0.39926673649471667
1103	26-27	0.39966065610627766
1103	28-29	0.4355266549786805
1103	30-31	0.40138148875636404
1103	32-33	0.4365575046760952
1103	34-35	0.46015590901459547
1103	36-37	0.4263495317316881
1103	38-39	0.4360844730006477
1103	40-41	0.43438062598912097
1103	42-43	0.4167331741711138
1103	44-45	0.5256616398481242
1103	46-47	0.4981487160305136
1103	48-49	0.4249977019962472
1103	50-51	0.45415937972406795
1103	52-53	0.3866781131200199
1103	54-55	0.306130451163547
1103	56-57	0.3289405759950981
1103	58-59	0.43300252167872344
1103	60-61	0.485908793231431
1103	62-63	0.4455512080206958
1103	64-65	0.4388888432563789
1103	66-67	0.44337593616098303
1103	68-69	0.3532595196929478
1103	70-71	0.4363219103709568
1103	72-73	0.44014109721048555
1103	74-75	0.3279831373732307
1103	76-77	0.3638794421120224
1103	78-79	0.3149489858334178
1103	80-81	0.29797182763751806
1103	82-83	0.32136349938014064
1103	84-85	0.3064848677860965
1103	86-87	0.35207597135423896
1103	88-89	0.34397770832679697
1103	90-91	0.34568472652848925
1103	92-93	0.3804247218749879
1103	94-95	0.4126557971961802
1103	96-97	0.3514161783410543
1103	98-99	0.39508525840680875
1103	100	0.6075040099495332
1104	1	0.19832885340563422
1104	2	0.2595896935893407
1104	3	0.2042949930582978
1104	4	0.15348018334266555
1104	5	0.11210601718498481
1104	6	0.16390074367210872
1104	7	0.2266443112859875
1104	8	0.2003543780346959
1104	9	0.18191089582139597
1104	10-11	0.2119811450120892
1104	12-13	0.27751376100574987
1104	14-15	0.3173099622194684
1104	16-17	0.3120275683409375
1104	18-19	0.2679208487827225
1104	20-21	0.26087046126959024
1104	22-23	0.35963651675075425
1104	24-25	0.39106231003113123
1104	26-27	0.3887754350034527
1104	28-29	0.38734250655728886
1104	30-31	0.3477356278788264
1104	32-33	0.4030205645946481
1104	34-35	0.39688954665628273
1104	36-37	0.376123173471953
1104	38-39	0.370679000298054
1104	40-41	0.41991209689362563
1104	42-43	0.3648378024786254
1104	44-45	0.3373758312825288
1104	46-47	0.36295590110111675
1104	48-49	0.3709790578761627
1104	50-51	0.3454051709698618
1104	52-53	0.2925880523905633
1104	54-55	0.3076098503032867
1104	56-57	0.24994845694244816
1104	58-59	0.2517154196373639
1104	60-61	0.254092016167597
1104	62-63	0.2979616563081109
1104	64-65	0.23238392165819732
1104	66-67	0.19021366885440472
1104	68-69	0.2687870958471663
1104	70-71	0.19842419962475333
1104	72-73	0.16686858698607665
1104	74-75	0.274379942466048
1104	76-77	0.22297057155655153
1104	78-79	0.2157099495279553
1104	80-81	0.16739087632685568
1104	82-83	0.17483299472627323
1104	84-85	0.20529377381849656
1104	86-87	0.21214384577912426
1104	88-89	0.23801728053226867
1104	90-91	0.20760998919000428
1104	92-93	0.21446010185277942
1104	94-95	0.20872983931898403
1104	96-97	0.15384017837631347
1104	98-99	0.14646996057685868
1104	100	0.42433406882802416
1105	1	0.22731499906621622
1105	2	0.2119286930365547
1105	3	0.14470924126172235
1105	4	0.18692321125863742
1105	5	0.17800395803627822
1105	6	0.1418201742967682
1105	7	0.1865574193567454
1105	8	0.20157051954934246
1105	9	0.19451886293029474
1105	10-11	0.17844915219838242
1105	12-13	0.22712378380840192
1105	14-15	0.324029835077404
1105	16-17	0.3110752984404428
1105	18-19	0.2812098724145642
1105	20-21	0.2915015938013781
1105	22-23	0.3105926734671698
1105	24-25	0.2475571794341107
1105	26-27	0.2706870574467928
1105	28-29	0.31422761227867824
1105	30-31	0.34686195803360675
1105	32-33	0.34708798789481676
1105	34-35	0.2667857254843682
1105	36-37	0.36164176108941604
1105	38-39	0.38946354977290554
1105	40-41	0.4076172344005329
1105	42-43	0.3452542963979184
1105	44-45	0.3689048182919308
1105	46-47	0.39781286627524537
1105	48-49	0.4210598172736155
1105	50-51	0.38762592483939784
1105	52-53	0.31566235103623086
1105	54-55	0.34700057031434284
1105	56-57	0.2598550869866685
1105	58-59	0.2922445448446638
1105	60-61	0.2604262305125431
1105	62-63	0.33315245578295816
1105	64-65	0.29824860863995184
1105	66-67	0.30065236081129143
1105	68-69	0.2916415734591098
1105	70-71	0.3054852485468089
1105	72-73	0.30929663770470484
1105	74-75	0.2990767708242501
1105	76-77	0.21431549623869728
1105	78-79	0.24471596113657768
1105	80-81	0.17409649752641343
1105	82-83	0.2299584093199698
1105	84-85	0.2254140923646517
1105	86-87	0.21490348508260126
1105	88-89	0.18970718172077028
1105	90-91	0.20209416553052506
1105	92-93	0.2120386155343681
1105	94-95	0.1914490557368893
1105	96-97	0.13200620587770118
1105	98-99	0.17341509601079963
1105	100	0.29126417524040704
1106	1	0.15349922061763976
1106	2	0.19713555561111207
1106	3	0.1676160351065974
1106	4	0.12729138973097776
1106	5	0.07511444737469475
1106	6	0.12141612751815245
1106	7	0.161876871873865
1106	8	0.12443305805069826
1106	9	0.11953966479787681
1106	10-11	0.17890021729642314
1106	12-13	0.1492944049953948
1106	14-15	0.19173951631164954
1106	16-17	0.1725295107324527
1106	18-19	0.21586386801713786
1106	20-21	0.1800657571552975
1106	22-23	0.23193256564216824
1106	24-25	0.18544121850185036
1106	26-27	0.2366258090059361
1106	28-29	0.17774217729823505
1106	30-31	0.21016074842290777
1106	32-33	0.21461016727294435
1106	34-35	0.24016503593891514
1106	36-37	0.1836573758807276
1106	38-39	0.22263336725739435
1106	40-41	0.18985385146798706
1106	42-43	0.1929947150762743
1106	44-45	0.18617601630980118
1106	46-47	0.21424184718790684
1106	48-49	0.27673720907738897
1106	50-51	0.2742715302228049
1106	52-53	0.24592676809603375
1106	54-55	0.2673183204988945
1106	56-57	0.21410123340424292
1106	58-59	0.12179775413929406
1106	60-61	0.13343762367818357
1106	62-63	0.197446580402179
1106	64-65	0.1694475744596602
1106	66-67	0.19378646510858744
1106	68-69	0.19467094320361866
1106	70-71	0.22900334418289958
1106	72-73	0.1786548304851152
1106	74-75	0.1275271496860526
1106	76-77	0.15698584749195277
1106	78-79	0.21265315036807664
1106	80-81	0.18905242381806175
1106	82-83	0.1700724576471231
1106	84-85	0.18874399937804753
1106	86-87	0.15498098098851898
1106	88-89	0.20664584816329068
1106	90-91	0.17658219298846234
1106	92-93	0.19629163492081148
1106	94-95	0.15882666279822644
1106	96-97	0.1803342143395099
1106	98-99	0.13368281299720763
1106	100	0.2688447072378928
1107	1	0.136684897109852
1107	2	0.1510194470937236
1107	3	0.1508012602991542
1107	4	0.11486047342144445
1107	5	-0.006716918668217886
1107	6	0.03671393817059965
1107	7	0.1186627130639053
1107	8	0.07849140339767047
1107	9	0.08987792849146814
1107	10-11	0.1385144463498449
1107	12-13	0.0590634762883937
1107	14-15	0.13659476659248782
1107	16-17	0.156789320228242
1107	18-19	0.14115429593814355
1107	20-21	0.12461092475140134
1107	22-23	0.09061801336527964
1107	24-25	0.07731318558565192
1107	26-27	0.13134285540365198
1107	28-29	0.16161640433143276
1107	30-31	0.11630932795060289
1107	32-33	0.24732297463953046
1107	34-35	0.2822612060374752
1107	36-37	0.11093184319116745
1107	38-39	0.17157266041859742
1107	40-41	0.15650222280052617
1107	42-43	0.20415501047578744
1107	44-45	0.15295495925185776
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2216	76-77	-0.7219393167479495
2216	78-79	-0.6932011744011248
2216	80-81	-0.754157954619302
2216	82-83	-0.6779990009900416
2216	84-85	-0.6886649488778573
2216	86-87	-0.7065470778588718
2216	88-89	-0.7022617204656001
2216	90-91	-0.7210945759362986
2216	92-93	-0.7344408607352975
2216	94-95	-0.7563330824335246
2216	96-97	-0.7678408637107275
2216	98-99	-0.7884996622165232
2216	100	-0.18681625423990766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	22.0
13	89.0
14	374.0
15	1115.0
16	2720.0
17	5507.0
18	9571.0
19	15212.0
20	21825.0
21	31239.0
22	43925.0
23	51795.0
24	51666.0
25	48076.0
26	47784.0
27	55304.0
28	69219.0
29	86070.0
30	106899.0
31	134263.0
32	170791.0
33	219735.0
34	316318.0
35	468656.0
36	748208.0
37	1409732.0
38	1123275.0
39	464650.0
40	937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.28144545967813	22.499286150120394	13.654418831543873	24.5648495586576
2	14.082171609380273	20.762657714708936	40.45614665550594	24.699024020404853
3	16.92745339555031	27.805614076541186	29.9747185836377	25.292213944270802
4	11.458481362921356	17.68865417025008	39.731966004862564	31.120898461966
5	12.151930464536791	39.23906803407357	35.27440021105349	13.33460129033615
6	28.770325009084168	39.85385397562375	17.71880317175576	13.65701784353632
7	25.35442274040878	32.97613085827981	24.387758697180587	17.281687704130828
8	26.577476769861285	34.49317359689717	19.521800335531353	19.407549297710194
9	26.373900281175345	14.14581398255314	21.902299061118697	37.57798667515282
10-11	25.659796237708765	26.012049821024817	27.295428328332733	21.03272561293368
12-13	26.001801414751252	24.01486390659497	28.013839885482117	21.969494793171663
14-15	23.305036686303403	24.747867671613978	26.08656933321296	25.860526308869662
16-17	21.17466298310196	27.01434939047124	27.594298736490096	24.216688889936705
18-19	20.609541244586527	27.09409622717279	30.63914871553427	21.65721381270641
20-21	22.310757914881748	26.27538110994585	29.703052472917964	21.710808502254434
22-23	22.903792317625598	25.789156878874792	29.535760066510303	21.771290736989307
24-25	22.265919904027726	25.916265102372822	30.41278314511477	21.405031848484686
26-27	22.18241587328826	26.236373638788073	29.144227574485136	22.43698291343853
28-29	21.14292755751509	26.84217528507106	29.81611156987201	22.198785587541835
30-31	22.255691999675374	26.267353037635004	29.686207543898917	21.7907474187907
32-33	22.89622875168208	26.605846715352776	28.68236546274212	21.81555907022302
34-35	22.609190109485027	26.414329863675267	29.67958175496115	21.296898271878558
36-37	22.302107579324105	25.915160804216526	29.290211483614055	22.492520132845314
38-39	22.483116069974642	25.871356977350146	30.10143241493705	21.54409453773816
40-41	21.881361217504494	25.08343147856233	30.68045274822125	22.35475455571193
42-43	22.158618933174363	25.382573579123225	29.52993182624096	22.928875661461447
44-45	22.38957500471956	26.621727574552835	29.035285130660384	21.953412290067224
46-47	22.438856501012012	26.362918649509965	28.212302547545733	22.985922301932295
48-49	23.64341791848211	26.05870203599276	28.024931196089874	22.272948849435256
50-51	22.791381776731594	26.122155358624333	27.682151789813148	23.404311074830925
52-53	21.904402486178306	26.89835426270482	27.568426608256893	23.628816642859984
54-55	22.164797744286965	26.839651174999528	27.319048038897936	23.676503041815565
56-57	23.500823227982707	26.10518772981014	27.042438879288117	23.351550162919036
58-59	22.10475464543819	26.6094955484933	28.120684416251525	23.165065389816984
60-61	22.338479304313196	27.36473618404689	27.086900026485626	23.20988448515429
62-63	22.893003499924014	26.349956292580117	26.674593657717704	24.082446549778165
64-65	21.785288842582336	26.678385675943243	27.22124510252103	24.31508037895339
66-67	22.66578451240834	26.119718172222882	27.15960030313901	24.054897012229766
68-69	22.09781242040946	25.975274939565967	27.658488257892532	24.268424382132032
70-71	22.121787033910287	25.73158172012185	27.70093272500787	24.44569852096
72-73	22.865064790590427	25.74316736683268	28.122256497486003	23.269511345090887
74-75	22.971991370310306	26.37236816550382	27.464015498103755	23.191624966082124
76-77	22.621821721949402	26.376342336935338	26.92342614587686	24.078409795238404
78-79	22.23813814548118	26.363999443643827	27.233976429534305	24.163885981340684
80-81	21.845480291695978	26.912745195820985	27.901162159873977	23.34061235260906
82-83	22.8378587062779	25.68463418195433	29.05127116111342	22.42623595065435
84-85	23.327553588697317	26.387774122297692	27.18040603465638	23.104266254348612
86-87	21.634883621873588	27.15976617625896	27.740346549795696	23.465003652071758
88-89	22.742038930541575	25.75428384424067	27.51267707990614	23.991000145311613
90-91	22.273606169766385	25.669279179019178	27.89615776108632	24.16095689012812
92-93	22.657332250536854	26.202874295286872	27.381072785343193	23.758720668833078
94-95	21.217406333167453	26.239719290914376	28.146351407655875	24.396522968262296
96-97	21.814743993012424	26.06240932266032	27.564044472716038	24.558802211611223
98-99	21.044662550146967	26.954068733971244	27.51787429265759	24.483394423224198
100	21.98556469577114	26.155718871134653	26.444337256415203	25.414379176679002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	869.0
1	554.0
2	344.5
3	431.5
4	301.5
5	238.5
6	475.5
7	1092.5
8	1789.0
9	1952.0
10	1478.5
11	991.5
12	948.5
13	1105.0
14	1383.5
15	2157.0
16	2832.5
17	3660.5
18	5168.0
19	6717.0
20	8601.5
21	10463.0
22	12489.0
23	14628.5
24	16149.5
25	17713.0
26	19926.5
27	21762.5
28	25781.0
29	37370.5
30	53501.5
31	67843.0
32	88437.0
33	108031.0
34	119476.0
35	134583.0
36	149248.5
37	178009.0
38	188799.0
39	169594.5
40	153675.5
41	137450.0
42	132223.0
43	141344.0
44	156179.0
45	194765.0
46	231988.5
47	255677.0
48	274187.0
49	266044.0
50	249525.0
51	255643.5
52	260970.5
53	251424.0
54	242636.5
55	207772.5
56	177239.0
57	144898.0
58	109986.5
59	108591.0
60	89911.0
61	57913.5
62	41318.0
63	34716.0
64	25274.0
65	10340.0
66	4520.0
67	3137.0
68	2464.0
69	1746.5
70	1254.5
71	933.0
72	700.5
73	603.0
74	496.5
75	401.0
76	273.5
77	148.0
78	78.5
79	35.5
80	17.0
81	6.5
82	5.0
83	2.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007993015226518721
3	0.0
4	0.08951826482857698
5	0.004662592215469254
6	3.5057084326836495E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.593351143500741E-4
14-15	0.009395298599592181
16-17	0.0
18-19	3.5057084326836495E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.764271081709124E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	4.382135540854562E-5
60-61	0.0
62-63	0.0
64-65	8.764271081709123E-5
66-67	1.3146406622563687E-4
68-69	0.0
70-71	5.346205359842566E-4
72-73	4.294492830037471E-4
74-75	4.382135540854562E-4
76-77	8.764271081709123E-5
78-79	4.382135540854562E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06452256370354258
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5704981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.68161627490524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69747879514279	42.19339801054961
2	16.383383076167867	19.555735640732543
3	6.115014758781405	10.948618930469221
4	2.8529110500860093	6.810653702306806
5	1.4814053381464405	4.4206332469426055
6	0.8296761001118647	2.9709846383601697
7	0.49682184796838696	2.0755791621207007
8	0.3239446828691811	1.5466833805835465
9	0.21295685195706118	1.1438648209451814
>10	0.5910552031978036	5.022897699720243
>50	0.007001990799305651	0.2881468245654466
>100	0.006579971009657603	0.7830783753973358
>500	9.532566680047548E-4	0.3988122035046832
>1k	6.808975786956889E-4	0.6917783544955444
>5k	4.539317191304588E-5	0.26666044030936514
>10k+	9.078634382609176E-5	0.8824745689969786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17810	0.312183335930479	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14764	0.25879139650070704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9843	0.17253344051452582	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.7528542163418248E-5
14-15	0.0	0.0	0.0	0.0	2.6292813245127373E-5
16-17	0.0	0.0	0.0	0.0	3.5057084326836495E-5
18-19	0.0	0.0	0.0	0.0	3.5057084326836495E-5
20-21	0.0	0.0	0.0	0.0	8.764271081709123E-5
22-23	0.0	0.0	0.0	9.640698189880036E-5	8.764271081709123E-5
24-25	0.0	0.0	0.0	1.6652115055247336E-4	8.764271081709123E-5
26-27	0.0	0.0	0.0	5.696776203110931E-4	1.0517125298050949E-4
28-29	0.0	0.0	0.0	0.0031639018604969938	1.0517125298050949E-4
30-31	0.0	0.0	0.0	0.011989522839778083	1.1393552406221861E-4
32-33	0.0	0.0	0.0	0.027344525774932467	1.2269979514392774E-4
34-35	0.0	0.0	0.0	0.051253457285834955	1.2269979514392774E-4
36-37	0.0	0.0	0.0	0.089036229919083	1.3146406622563687E-4
38-39	0.0	0.0	0.0	0.14865080181686846	1.5775687947076423E-4
40-41	0.0	0.0	0.0	0.21230570268332183	1.5775687947076423E-4
42-43	0.0	0.0	0.0	0.2785372992477977	1.6652115055247336E-4
44-45	0.0	0.0	0.0	0.36037806260879746	1.7528542163418247E-4
46-47	0.0	0.0	0.0	0.46248182071070876	1.7528542163418247E-4
48-49	0.0	0.0	0.0	0.5820527710784663	1.9281396379760071E-4
50-51	0.0	0.0	0.0	0.7118516257985785	2.1034250596101899E-4
52-53	0.0	0.0	0.0	0.8361289897372138	2.1034250596101899E-4
54-55	0.0	0.0	0.0	0.9716421492026003	2.1034250596101899E-4
56-57	0.0	0.0	0.0	1.1400826751219681	2.1034250596101899E-4
58-59	0.0	0.0	0.0	1.3474453289152057	2.1034250596101899E-4
60-61	0.0	0.0	0.0	1.5591375326228079	2.1910677704272812E-4
62-63	0.0	0.0	0.0	1.7871312805423893	2.453995902878555E-4
64-65	0.0	0.0	0.0	2.0259664317900445	2.453995902878555E-4
66-67	0.0	0.0	0.0	2.295686874329643	2.453995902878555E-4
68-69	0.0	0.0	0.0	2.5990796463651673	2.453995902878555E-4
70-71	0.0	0.0	0.0	2.935662712987125	2.453995902878555E-4
72-73	0.0	0.0	0.0	3.2855850001954434	2.453995902878555E-4
74-75	0.0	0.0	0.0	3.6306869383088216	2.453995902878555E-4
76-77	0.0	0.0	0.0	4.035175577271861	2.453995902878555E-4
78-79	0.0	0.0	0.0	4.464791381426161	2.453995902878555E-4
80-81	0.0	0.0	0.0	4.936861314700259	2.453995902878555E-4
82-83	0.0	0.0	0.0	5.436976915435827	2.6292813245127375E-4
84-85	0.0	0.0	0.0	5.924322973205344	2.6292813245127375E-4
86-87	0.0	0.0	0.0	6.443413220832813	2.716924035329829E-4
88	0.0	0.0	0.0	6.859374290641809	2.8045667461469196E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13110	0.0	46.10155	1
GGGCACC	6545	0.0	43.03915	94
GTGGTAT	2480	0.0	42.29298	1
GTATCAA	22890	0.0	39.8631	1
GTACTAG	6885	0.0	34.77194	1
CTGTGCG	9175	0.0	32.893337	9
CAACGCA	27130	0.0	32.655247	5
ATCAACG	27670	0.0	32.004463	3
TAGTACT	9375	0.0	31.653917	4
TAGGCAT	8370	0.0	31.557476	5
ACTGTGC	9920	0.0	31.363905	8
TATTCTC	3710	0.0	31.163961	5
TATCAAC	29970	0.0	30.430294	2
GTACTGT	10140	0.0	29.848349	6
AACGCAG	31340	0.0	29.362028	6
TACTGTG	11310	0.0	29.336918	7
TACCCCG	485	0.0	29.071737	5
CTAACGC	3380	0.0	28.802023	3
GAACCGT	720	0.0	28.72054	6
CTAGGCA	9640	0.0	28.295694	4
>>END_MODULE