FastQCFastQC Report
Mon 16 Jan 2017
ERR1146668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5653609
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC77990.1379472828771852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC56700.042.30582494
GTACTAG64550.037.3684731
GCATCGT68600.032.0580448
CTAACGC31500.031.9269143
CAACGCA99450.031.6117215
CTGTGCG83300.031.5339419
TCTAACG32200.031.37882
CGCCTAC32050.030.9363987
TAGGCAT83450.030.8589445
TAGTACT89150.030.3107284
TAACGCC33250.029.9636084
ATCAACG105000.029.8979133
GTATCAA117650.029.694851
TATTCTC31550.029.0443785
ACTGTGC95900.028.812048
CTAGGCA93400.028.730234
GTACTGT97650.027.7182356
CTAGTAC102050.027.5386053
TACTGTG104800.027.3965077
ACGCCTA36400.027.3684256
Produced by FastQC (version 0.11.5)