##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146668_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5653609
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47883626193463	31.0	31.0	34.0	28.0	34.0
2	31.541124085517765	33.0	31.0	34.0	28.0	34.0
3	31.676663349021837	33.0	31.0	34.0	28.0	34.0
4	35.10862477401603	37.0	35.0	37.0	32.0	37.0
5	35.262350296951915	37.0	35.0	37.0	32.0	37.0
6	35.32635702256736	37.0	35.0	37.0	32.0	37.0
7	35.276093553692874	37.0	35.0	37.0	32.0	37.0
8	35.224632442745865	37.0	35.0	37.0	32.0	37.0
9	36.753904983524684	39.0	37.0	39.0	32.0	39.0
10-11	36.59498039217073	39.0	37.0	39.0	32.0	39.0
12-13	36.5267282367776	39.0	37.0	39.0	32.0	39.0
14-15	37.722957318060026	40.0	38.0	41.0	32.0	41.0
16-17	37.75378081505106	40.0	38.0	41.0	32.0	41.0
18-19	37.72628713800336	40.0	37.5	41.0	32.0	41.0
20-21	37.79768692882723	40.0	38.0	41.0	32.0	41.0
22-23	37.739225422203766	40.0	37.5	41.0	31.5	41.0
24-25	37.697129833350694	40.0	37.0	41.0	31.5	41.0
26-27	37.73867179353931	40.0	37.0	41.0	32.0	41.0
28-29	37.731634341886746	40.0	37.0	41.0	32.0	41.0
30-31	37.603341776907456	40.0	37.0	41.0	31.0	41.0
32-33	37.597037308381246	40.0	37.0	41.0	31.0	41.0
34-35	37.54006953788279	40.0	37.0	41.0	31.0	41.0
36-37	37.42449274436913	40.0	36.5	41.0	31.0	41.0
38-39	37.31904213750898	40.0	36.0	41.0	30.0	41.0
40-41	37.23692467943928	40.0	36.0	41.0	30.0	41.0
42-43	37.0753386022981	40.0	36.0	41.0	30.0	41.0
44-45	36.913337657414935	39.0	35.0	41.0	30.0	41.0
46-47	36.911753182789965	39.0	35.0	41.0	30.0	41.0
48-49	36.77151099412782	39.0	35.0	41.0	30.0	41.0
50-51	36.59354086920408	39.0	35.0	41.0	30.0	41.0
52-53	36.375195118728584	39.0	35.0	41.0	29.0	41.0
54-55	36.15954428401398	38.0	35.0	40.0	29.0	41.0
56-57	35.919521141274544	38.0	34.5	40.0	29.0	41.0
58-59	35.62208785573958	37.5	34.0	40.0	28.0	41.0
60-61	35.377608709763976	37.0	34.0	40.0	28.0	41.0
62-63	35.072802523131685	36.0	34.0	40.0	28.0	41.0
64-65	34.919013854689986	36.0	34.0	39.5	28.0	41.0
66-67	34.66499646508982	35.5	33.5	39.0	28.0	41.0
68-69	34.54089847741505	35.0	33.5	39.0	28.0	41.0
70-71	34.454211283447435	35.0	34.0	39.0	28.0	41.0
72-73	34.231314280842554	35.0	33.5	38.0	28.0	40.5
74-75	33.973337915657055	35.0	33.0	37.0	27.0	40.0
76-77	33.706917828947844	35.0	33.0	37.0	27.0	39.0
78-79	33.44885152121415	35.0	33.0	36.0	27.0	39.0
80-81	33.25712381241787	35.0	33.0	36.0	26.5	39.0
82-83	32.991094272702625	35.0	33.0	36.0	26.0	37.0
84-85	32.80621935475199	35.0	33.0	35.0	26.0	37.0
86-87	32.625663447896734	35.0	33.0	35.0	26.0	36.5
88-89	32.49187306727437	35.0	33.0	35.0	26.0	36.0
90-91	32.33417601040327	35.0	33.0	35.0	25.0	36.0
92-93	32.1791741346103	35.0	32.0	35.0	25.0	36.0
94-95	32.10659642362958	35.0	32.0	35.0	25.0	36.0
96-97	31.995013981334758	35.0	32.0	35.0	25.0	35.0
98-99	31.899587060230026	35.0	32.0	35.0	25.0	35.0
100	30.946940264174618	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34845270379612003
1101	2	0.4395547437963252
1101	3	0.46278277131704115
1101	4	0.5563537751990921
1101	5	0.44887101140422914
1101	6	0.41647912247466934
1101	7	0.4557429094374612
1101	8	0.4171857726721413
1101	9	0.5966198918885723
1101	10-11	0.6353267802819147
1101	12-13	0.5671199565305258
1101	14-15	0.7446830852212827
1101	16-17	0.7363735121629844
1101	18-19	0.6792002231653669
1101	20-21	0.6966498616514869
1101	22-23	0.7184179796982662
1101	24-25	0.7706465937698752
1101	26-27	0.6941593297752959
1101	28-29	0.6900318919297845
1101	30-31	0.7428460945572084
1101	32-33	0.7141897066695506
1101	34-35	0.7182831909828451
1101	36-37	0.7639974528259259
1101	38-39	0.7076941136393629
1101	40-41	0.727162326215975
1101	42-43	0.6479944801456483
1101	44-45	0.66812085020387
1101	46-47	0.6798438351229592
1101	48-49	0.6272404037791901
1101	50-51	0.6257764103125893
1101	52-53	0.6042728117463412
1101	54-55	0.5977903200913417
1101	56-57	0.5629600612545858
1101	58-59	0.5372224075083167
1101	60-61	0.5729148165034772
1101	62-63	0.6001325069730754
1101	64-65	0.6264318411597856
1101	66-67	0.5823004540803183
1101	68-69	0.5884333487005549
1101	70-71	0.5936673224526743
1101	72-73	0.5757024392230647
1101	74-75	0.5406144028464368
1101	76-77	0.49837176702080654
1101	78-79	0.54628375728295
1101	80-81	0.4861640792222488
1101	82-83	0.45036926708277036
1101	84-85	0.4872996938194234
1101	86-87	0.5007343187279645
1101	88-89	0.48717354066359775
1101	90-91	0.49428887610697814
1101	92-93	0.43592739686302906
1101	94-95	0.44825840255577276
1101	96-97	0.45243062744681595
1101	98-99	0.43191225752068974
1101	100	0.510155614630321
1102	1	0.38889474859185924
1102	2	0.4782829128137607
1102	3	0.4707372590707557
1102	4	0.4859469729913428
1102	5	0.39655219693782584
1102	6	0.4430197969529317
1102	7	0.38359210382210307
1102	8	0.40513657772746825
1102	9	0.5806643024099856
1102	10-11	0.6311710841077982
1102	12-13	0.5549797348172305
1102	14-15	0.7078768627937038
1102	16-17	0.624316864673041
1102	18-19	0.6483579236803507
1102	20-21	0.5775549761954082
1102	22-23	0.653351726540798
1102	24-25	0.6888389545800422
1102	26-27	0.6270561074711054
1102	28-29	0.681239034010737
1102	30-31	0.6932991717254353
1102	32-33	0.6687136000582896
1102	34-35	0.6558186802295722
1102	36-37	0.7158504240652874
1102	38-39	0.6980115997049197
1102	40-41	0.586734406366908
1102	42-43	0.6831532213815521
1102	44-45	0.6519369376181956
1102	46-47	0.6634286720189024
1102	48-49	0.635118868683044
1102	50-51	0.572634529079032
1102	52-53	0.6134619408291968
1102	54-55	0.5939653390367923
1102	56-57	0.576650163817753
1102	58-59	0.6078209813603834
1102	60-61	0.6108104252562541
1102	62-63	0.6597880804879139
1102	64-65	0.6353781298708512
1102	66-67	0.6440092584741919
1102	68-69	0.5631238116422779
1102	70-71	0.5213579948952258
1102	72-73	0.5594353612034482
1102	74-75	0.5279146862003898
1102	76-77	0.5414583273683888
1102	78-79	0.5002891095491293
1102	80-81	0.49465631970308266
1102	82-83	0.47193787947170307
1102	84-85	0.5080464223848935
1102	86-87	0.455340905870564
1102	88-89	0.4455252152394422
1102	90-91	0.48802527159936915
1102	92-93	0.4624618721173661
1102	94-95	0.49682551858082036
1102	96-97	0.4795572558642718
1102	98-99	0.437303500949195
1102	100	0.4893730308917199
1103	1	0.30285615817611244
1103	2	0.36364138633456733
1103	3	0.4141026766561531
1103	4	0.45124818337576755
1103	5	0.32785339220426835
1103	6	0.35188149435629157
1103	7	0.3280993616685848
1103	8	0.3733864089680665
1103	9	0.49690598957495524
1103	10-11	0.5807291438253515
1103	12-13	0.4700339139682157
1103	14-15	0.6476468705342739
1103	16-17	0.62995247736999
1103	18-19	0.6657697778045133
1103	20-21	0.562176832836343
1103	22-23	0.6181465471635263
1103	24-25	0.6047368191422464
1103	26-27	0.5530053475231611
1103	28-29	0.5744987749236117
1103	30-31	0.578644793563754
1103	32-33	0.567353621489012
1103	34-35	0.5735654158673498
1103	36-37	0.5798388298533297
1103	38-39	0.6739261032560648
1103	40-41	0.5472890155297421
1103	42-43	0.5725409379400688
1103	44-45	0.5503633268488173
1103	46-47	0.537059305375756
1103	48-49	0.498635074508222
1103	50-51	0.442059050122694
1103	52-53	0.4579012500242783
1103	54-55	0.5255636431669828
1103	56-57	0.5536212737096946
1103	58-59	0.540184556810857
1103	60-61	0.5561164538710344
1103	62-63	0.5261339646037513
1103	64-65	0.4727715581828775
1103	66-67	0.48506120445879475
1103	68-69	0.4645062761233092
1103	70-71	0.47042582882286865
1103	72-73	0.4700056768934857
1103	74-75	0.4495434876590352
1103	76-77	0.3670646987569981
1103	78-79	0.439552922778752
1103	80-81	0.4226676097701727
1103	82-83	0.41572282116020887
1103	84-85	0.4423788435160745
1103	86-87	0.4168971861844639
1103	88-89	0.519738946794277
1103	90-91	0.45732097736342325
1103	92-93	0.42295102347035396
1103	94-95	0.3680985341450693
1103	96-97	0.37810344536900686
1103	98-99	0.3714519819389004
1103	100	0.4834691646007627
1104	1	0.20901626456502598
1104	2	0.29911744669946216
1104	3	0.23918740821999762
1104	4	0.3478979934868178
1104	5	0.23845631608809725
1104	6	0.20610180305298798
1104	7	0.2659421268617592
1104	8	0.2962457129280125
1104	9	0.3043366206356879
1104	10-11	0.44098393662837054
1104	12-13	0.3513375010792501
1104	14-15	0.4482213756313769
1104	16-17	0.45662414510053395
1104	18-19	0.3759280048456901
1104	20-21	0.39310928354147023
1104	22-23	0.4009885018468893
1104	24-25	0.47270187293482024
1104	26-27	0.4351437300927472
1104	28-29	0.41578622299720536
1104	30-31	0.39129585445290616
1104	32-33	0.49293878019448556
1104	34-35	0.46501340795664703
1104	36-37	0.41439182277470366
1104	38-39	0.3999588401975771
1104	40-41	0.4428284649318499
1104	42-43	0.41036405628911865
1104	44-45	0.45103440579398324
1104	46-47	0.42365452157271477
1104	48-49	0.399691812728868
1104	50-51	0.3472422590332016
1104	52-53	0.3874008988932047
1104	54-55	0.44302216740947387
1104	56-57	0.4437469110865777
1104	58-59	0.4314529672029934
1104	60-61	0.43004437472387025
1104	62-63	0.4397623111103641
1104	64-65	0.3942140198297537
1104	66-67	0.31401459073753557
1104	68-69	0.35483342235841775
1104	70-71	0.34264356588270317
1104	72-73	0.3494241076656337
1104	74-75	0.343874422993828
1104	76-77	0.29941002682694773
1104	78-79	0.3084544294213387
1104	80-81	0.2704056728366169
1104	82-83	0.2735565427134574
1104	84-85	0.30622722443444417
1104	86-87	0.2697591699211159
1104	88-89	0.24253572263098278
1104	90-91	0.3067856257932746
1104	92-93	0.30321701246779753
1104	94-95	0.3185828669930615
1104	96-97	0.36205699525913104
1104	98-99	0.3623840428877294
1104	100	0.4234115464365473
1105	1	0.09920922388775111
1105	2	0.17494243775200857
1105	3	0.15956259827163777
1105	4	0.23536444992517147
1105	5	0.14745597244565545
1105	6	0.15010520907245706
1105	7	0.15527347418570514
1105	8	0.17244747998855559
1105	9	0.2838954919983081
1105	10-11	0.29080724839369054
1105	12-13	0.18409515954166267
1105	14-15	0.3343170350837852
1105	16-17	0.2767362518336185
1105	18-19	0.32319591658833247
1105	20-21	0.24870252783515667
1105	22-23	0.3356911202730828
1105	24-25	0.3323290178252307
1105	26-27	0.35135812354880613
1105	28-29	0.33563900247760614
1105	30-31	0.3819952478413029
1105	32-33	0.3238306707062506
1105	34-35	0.3448855530333361
1105	36-37	0.3384798240848994
1105	38-39	0.33984409168887453
1105	40-41	0.32786787355278335
1105	42-43	0.345024812261272
1105	44-45	0.2884585932655952
1105	46-47	0.3199530787478082
1105	48-49	0.2135197066161325
1105	50-51	0.25365453172926067
1105	52-53	0.32439056884889794
1105	54-55	0.27768211035624546
1105	56-57	0.2996954502553848
1105	58-59	0.3438474221694392
1105	60-61	0.3518894283470715
1105	62-63	0.2836217197546844
1105	64-65	0.345580233802778
1105	66-67	0.33222037291834283
1105	68-69	0.29252250296537596
1105	70-71	0.255038229674156
1105	72-73	0.2870745834887316
1105	74-75	0.24782183662366464
1105	76-77	0.26692785310446965
1105	78-79	0.3384702683808385
1105	80-81	0.24248977819602402
1105	82-83	0.25980996807012247
1105	84-85	0.20259580759684326
1105	86-87	0.2248309698072788
1105	88-89	0.220683117895355
1105	90-91	0.24883458284041637
1105	92-93	0.21817660427114305
1105	94-95	0.25291065844469784
1105	96-97	0.1626013909276196
1105	98-99	0.1689174424436466
1105	100	0.2396235397971367
1106	1	0.0959032015229937
1106	2	0.14461860656932402
1106	3	0.14396371056327695
1106	4	0.2618481488483724
1106	5	0.09344170624825665
1106	6	0.12531231591282932
1106	7	0.1563057091959692
1106	8	0.1295067936930394
1106	9	0.22548016759207457
1106	10-11	0.2652569214054239
1106	12-13	0.17753141610381107
1106	14-15	0.3243742363761015
1106	16-17	0.23216332834761744
1106	18-19	0.28190826964332416
1106	20-21	0.24556620433709497
1106	22-23	0.23983909069914233
1106	24-25	0.2997565815914669
1106	26-27	0.30884142386486957
1106	28-29	0.31318751425558844
1106	30-31	0.3205124217074484
1106	32-33	0.2620042568304797
1106	34-35	0.2796215927769907
1106	36-37	0.2686205371063224
1106	38-39	0.27149896112243255
1106	40-41	0.3115109041489177
1106	42-43	0.26666691336563275
1106	44-45	0.2905302039057247
1106	46-47	0.3021033466224523
1106	48-49	0.1752830548777311
1106	50-51	0.24051965776289563
1106	52-53	0.19215346287131752
1106	54-55	0.24453262896381744
1106	56-57	0.24880929654773354
1106	58-59	0.29445419314999555
1106	60-61	0.29051274674876737
1106	62-63	0.26607343585683196
1106	64-65	0.20633943349115214
1106	66-67	0.2745765452769504
1106	68-69	0.27908734974866434
1106	70-71	0.22569024795831183
1106	72-73	0.13335514882637511
1106	74-75	0.16670532061279886
1106	76-77	0.21121211950833896
1106	78-79	0.1581186958206402
1106	80-81	0.20622575430788004
1106	82-83	0.19528663231404408
1106	84-85	0.20137966994304435
1106	86-87	0.1678572970604364
1106	88-89	0.230011450090835
1106	90-91	0.22768308173711205
1106	92-93	0.24749336162086166
1106	94-95	0.2399289064036907
1106	96-97	0.25588690999616404
1106	98-99	0.22078586795054989
1106	100	0.19083151714523083
1107	1	0.14970053014690876
1107	2	0.1896216543337097
1107	3	0.19936897761757066
1107	4	0.25362580148024705
1107	5	0.16304075678141317
1107	6	0.2547575709429637
1107	7	0.20863145278445216
1107	8	0.23294348257567066
1107	9	0.23413964790280772
1107	10-11	0.2897342462193393
1107	12-13	0.21922496388763335
1107	14-15	0.3924785172056602
1107	16-17	0.3173860771062138
1107	18-19	0.29608403335534206
1107	20-21	0.27939216258292277
1107	22-23	0.3672330089596443
1107	24-25	0.3534450557002913
1107	26-27	0.3072099969486004
1107	28-29	0.268552058932336
1107	30-31	0.3218394975299006
1107	32-33	0.3645847401037443
1107	34-35	0.311995545351877
1107	36-37	0.2764400221712009
1107	38-39	0.24270611482269544
1107	40-41	0.24080235854488308
1107	42-43	0.3645333810735494
1107	44-45	0.405395091032311
1107	46-47	0.3504663888732793
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2209	3	0.14112193693144093
2209	4	0.14163716196063092
2209	5	0.09743987360792516
2209	6	0.045342738834335705
2209	7	0.054996112995645774
2209	8	0.09098060237040073
2209	9	0.12758907563468824
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2214	96-97	-0.5978232417043969
2214	98-99	-0.5897447366014141
2214	100	-0.43018017881651716
2215	1	-0.9094962095070755
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2215	3	-0.953727883797697
2215	4	-2.4645058273434515
2215	5	-1.1421344113610346
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2215	9	-1.1031620702482385
2215	10-11	-0.8366880890362722
2215	12-13	-0.9969572377441196
2215	14-15	-1.0213994345547022
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2215	24-25	-1.0678096671263972
2215	26-27	-1.064508097457967
2215	28-29	-0.9576162647653561
2215	30-31	-3.627975367259239
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2216	1	-0.5534731555946166
2216	2	-0.6402986618580186
2216	3	-0.6278351751274442
2216	4	-0.47971289409125006
2216	5	-0.44800908403604467
2216	6	-0.3674648727286183
2216	7	-0.5176409992152102
2216	8	-0.5975320388070813
2216	9	-0.6324310916447402
2216	10-11	-1.0653339823296903
2216	12-13	-0.6010088724046625
2216	14-15	-0.7372797217783855
2216	16-17	-0.75511553512424
2216	18-19	-0.981792095621195
2216	20-21	-1.1016522305801786
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2216	24-25	-1.0283658409371128
2216	26-27	-1.4528617692540209
2216	28-29	-1.2969266940293522
2216	30-31	-1.3870432516753795
2216	32-33	-1.615052670571096
2216	34-35	-1.2486653671112435
2216	36-37	-1.3116901060622155
2216	38-39	-1.193511390844435
2216	40-41	-1.1048119593902754
2216	42-43	-1.1043745113343348
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2216	50-51	-1.1999464332334213
2216	52-53	-1.1768024192340931
2216	54-55	-1.2541784249169083
2216	56-57	-1.166718232951169
2216	58-59	-1.1578510131318822
2216	60-61	-1.1782815436786152
2216	62-63	-1.2128401125954227
2216	64-65	-1.1298794054053118
2216	66-67	-1.1667168614459271
2216	68-69	-1.098323059448596
2216	70-71	-1.0692074106456033
2216	72-73	-0.966893550865592
2216	74-75	-1.090506549642548
2216	76-77	-1.113569900050983
2216	78-79	-0.9050628086652139
2216	80-81	-1.015182197349283
2216	82-83	-0.9225957592427889
2216	84-85	-0.9408643503232454
2216	86-87	-0.9126153585977974
2216	88-89	-0.9865201360324782
2216	90-91	-1.1260743455887123
2216	92-93	-1.0002376219001512
2216	94-95	-0.9963257014840039
2216	96-97	-1.0587333495604732
2216	98-99	-0.985003658888818
2216	100	-0.649922409027571
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12.0
3	22.0
4	44.0
5	30.0
6	63.0
7	179.0
8	548.0
9	920.0
10	1368.0
11	2254.0
12	3940.0
13	5982.0
14	8461.0
15	11501.0
16	14225.0
17	17231.0
18	19433.0
19	22519.0
20	25879.0
21	29814.0
22	34762.0
23	40170.0
24	47718.0
25	55734.0
26	63003.0
27	69562.0
28	79433.0
29	97969.0
30	115691.0
31	133501.0
32	164671.0
33	215781.0
34	312890.0
35	450600.0
36	725180.0
37	1279944.0
38	1143178.0
39	458445.0
40	952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13128715615376	20.83335619344004	11.759710840592966	24.275645809813234
2	15.706610405836136	19.946851275022805	40.240232378409864	24.106305940731193
3	18.476764985162365	26.839137959581066	29.10331012992306	25.58078692533351
4	11.883972703205558	16.503458799197187	40.84381398017873	30.76875451741853
5	12.734745946642011	38.906983214876576	35.57235213718331	12.785918701298105
6	29.514023393016902	39.047121756114194	18.25347635315722	13.185378497711675
7	26.520967628960946	31.979994240809173	24.119070209064287	17.379967921165594
8	26.18575348242233	35.142448902775186	20.06298359115428	18.60881402364821
9	26.169003063192903	14.744768711071114	21.531338207414844	37.55489001832114
10-11	25.533719059223152	25.898982998922982	28.003082293122787	20.56421564873108
12-13	25.54602435194321	23.892722045481158	27.89004657922023	22.671207023355407
14-15	22.937374886189822	25.1216193568614	25.2051059479332	26.735899809015578
16-17	21.953485073094352	27.612825353408628	26.545935559803404	23.887754013693616
18-19	21.24718085251745	28.555424653974825	28.06310362213744	22.134290871370286
20-21	23.08135867203779	27.67592020927682	26.975618656159313	22.267102462526076
22-23	23.547610650023895	27.374244412267984	26.56959012980526	22.508554807902865
24-25	22.918664125131542	27.33870123994026	27.616031977141397	22.1266026577868
26-27	22.889262507705517	27.58377016369752	26.404076120362646	23.122891208234314
28-29	22.00749072547786	28.209281827490795	26.958704961323214	22.824522485708133
30-31	22.84018800589068	27.791072293895276	26.986396610070855	22.382343090143188
32-33	23.278039401072565	27.912549766529033	26.004725581510534	22.80468525088787
34-35	23.16224700657521	27.698473449537076	27.121912218815737	22.01736732507198
36-37	22.787071765133128	27.277042625766008	26.75444267886889	23.181442930231974
38-39	23.02775830285472	27.398166887673998	27.399555461726077	22.1745193477452
40-41	22.589379133091175	26.46578417403625	28.042808587189267	22.90202810568331
42-43	22.542879361792913	26.7817162924946	27.280227128471445	23.395177217241045
44-45	22.706532182719886	27.829653352902184	27.131345465147323	22.33246899923061
46-47	22.637179817637872	27.472230030822992	26.737727194163252	23.15286295737589
48-49	23.387419002634534	27.057748612696347	26.97428880701837	22.580543577650747
50-51	22.918855001947332	26.880816954523702	26.95403985084041	23.246288192688557
52-53	22.317442386601023	27.607927678006305	26.773645234241926	23.300984701150742
54-55	22.368332713703843	27.437282305938105	26.769073155465616	23.425311824892443
56-57	23.55798122235987	26.796348420699385	26.493055549413057	23.152614807527687
58-59	22.364565867410715	27.292923667630575	27.475784027170153	22.86672643778856
60-61	22.348569314294615	27.97660562562155	26.724316133108513	22.950508926975328
62-63	22.85044906682743	27.183749585728854	26.26698865496297	23.698812692480743
64-65	21.938413411394762	27.475152945771647	26.7989879327449	23.78744571008869
66-67	22.705177359860382	26.966454128667174	26.72752246358333	23.600846047889114
68-69	22.105871928060814	27.067929249846618	27.09077419054131	23.73542463155126
70-71	22.13123547987062	26.715569841805713	27.21867930647789	23.934515371845773
72-73	22.80461568723736	26.749801349900835	27.725300699290806	22.720282263571004
74-75	23.004079515662436	27.04250194962869	27.114808695808307	22.83860983890057
76-77	22.651197186779783	26.974069716851524	26.84868548662499	23.526047609743706
78-79	22.47813924756662	26.941737740315258	26.910037415935324	23.6700855961828
80-81	22.028490097030772	27.31720847428964	27.57782875515739	23.076472673522197
82-83	22.96599686588636	26.217431919526973	28.64298256956634	22.173588645020327
84-85	23.231273645013005	26.807541921652255	27.232598247784743	22.728586185549997
86-87	21.81427423007557	27.52138714601634	27.73225361962681	22.932085004281276
88-89	23.02813032221819	26.238708141317783	27.43756266375716	23.29559887270687
90-91	22.64120937046112	26.307589664510633	27.612320172782372	23.438880792245882
92-93	23.036290103604387	26.487859222990977	27.529926294046547	22.945924379358086
94-95	21.768129280577707	26.62919659907308	28.178569271142383	23.424104849206827
96-97	22.17004140825754	26.51376470553897	27.74577910754976	23.570414778653728
98-99	21.562439137688173	27.24608796296706	27.509473072346307	23.681999826998464
100	22.454419402324174	26.41396810151732	26.706125842934934	24.42548665322357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	921.0
1	624.5
2	396.5
3	448.5
4	385.5
5	329.0
6	320.5
7	348.0
8	361.0
9	355.0
10	351.5
11	321.5
12	306.5
13	355.5
14	395.0
15	638.0
16	806.0
17	860.5
18	1231.5
19	1622.5
20	2154.0
21	2892.0
22	4078.5
23	5712.0
24	7799.5
25	10437.0
26	14145.0
27	18236.5
28	23788.0
29	35495.5
30	51660.0
31	66616.5
32	86487.0
33	105357.0
34	119009.5
35	136185.0
36	151150.5
37	176970.5
38	188023.0
39	174691.0
40	162707.5
41	150592.0
42	148955.5
43	157971.0
44	172361.5
45	205228.0
46	236266.5
47	257299.0
48	270932.5
49	264146.0
50	250040.0
51	255818.5
52	257992.0
53	242826.5
54	228310.0
55	195966.0
56	171217.0
57	140423.0
58	108187.0
59	104841.0
60	86741.5
61	58031.0
62	42283.5
63	34627.0
64	24673.0
65	11047.0
66	5667.5
67	4039.5
68	3028.5
69	2253.0
70	1700.5
71	1306.5
72	1002.0
73	887.5
74	741.5
75	545.5
76	373.0
77	231.5
78	135.0
79	77.5
80	51.5
81	43.0
82	31.0
83	23.0
84	23.0
85	18.5
86	15.5
87	17.5
88	14.0
89	10.0
90	10.5
91	8.0
92	7.5
93	9.0
94	8.5
95	5.5
96	4.0
97	6.0
98	5.0
99	4.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05836979529359035
2	0.007358131770343509
3	0.007375819587099143
4	0.008684718027016017
5	0.0037144415186830216
6	0.0022463527279654467
7	6.544492199584372E-4
8	0.0012912106231612408
9	5.483223194246365E-4
10-11	4.598832356464693E-4
12-13	2.6531725133450154E-4
14-15	1.1497080891161733E-4
16-17	4.598832356464693E-4
18-19	0.0014592448823397587
20-21	7.871078456256881E-4
22-23	0.0011143324556049064
24-25	0.0012469910812721574
26-27	0.0034314364505928867
28-29	0.0038559440527280893
30-31	0.004872993516177012
32-33	0.005951950338270651
34-35	0.003121899657369302
36-37	0.007641136838433644
38-39	0.00579275998746995
40-41	0.005377096293712565
42-43	0.013672682352104646
44-45	0.019244344630129178
46-47	0.01786469492318977
48-49	0.021004282397314705
50-51	0.017785099747789423
52-53	0.02452415793168576
54-55	0.01835110988396969
56-57	0.014539385373130684
58-59	0.016538108666517264
60-61	0.015609498286846508
62-63	0.010763036495802947
64-65	0.011054885472270898
66-67	0.01304476485727966
68-69	0.004926056966443912
70-71	0.008233678699747365
72-73	0.008012580990301946
74-75	0.01078072431255858
76-77	0.010356216710423378
78-79	0.011850837226274403
80-81	0.009852113932887823
82-83	0.005943106429892835
84-85	0.010497719244468445
86-87	0.008941191369972701
88-89	0.0074465708541216775
90-91	0.01269100852216699
92-93	0.008313273875147714
94-95	0.0068186533592966905
96-97	0.009011942636995236
98-99	0.00849015204270405
100	0.25703935309286513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5653609.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.36634971749955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67424159872816	46.777756501977905
2	15.80188679282666	20.342195430068365
3	5.636956428535923	10.884909264643513
4	2.57554077891432	6.631126339491213
5	1.3177024470087633	4.240784826388548
6	0.7002929490151131	2.7045180516603513
7	0.42453316648731687	1.912795518256007
8	0.2727126479544994	1.4042814136499717
9	0.16737836422931576	0.969618089441444
>10	0.42690863789168904	3.7369729406036343
>50	0.001347737099335263	0.056842917606237636
>100	3.752300253931912E-4	0.05489251942447579
>500	0.0	0.0
>1k	8.214752228150968E-5	0.07711871471271302
>5k	4.10737611407548E-5	0.20618747207557364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7799	0.1379472828771852	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.075126702253375E-5
2	0.0	0.0	0.0	0.0	7.075126702253375E-5
3	0.0	0.0	0.0	0.0	7.075126702253375E-5
4	0.0	0.0	0.0	0.0	7.075126702253375E-5
5	0.0	0.0	0.0	0.0	7.075126702253375E-5
6	0.0	0.0	0.0	0.0	1.415025340450675E-4
7	0.0	0.0	0.0	0.0	1.415025340450675E-4
8	0.0	0.0	0.0	0.0	1.945659843119678E-4
9	0.0	0.0	0.0	0.0	2.4762943457886814E-4
10-11	0.0	0.0	0.0	0.0	2.4762943457886814E-4
12-13	0.0	0.0	0.0	0.0	4.3335151051301923E-4
14-15	0.0	0.0	0.0	0.0	4.5103932726865264E-4
16-17	0.0	0.0	0.0	0.0	5.129466859133697E-4
18-19	0.0	0.0	0.0	1.7687816755633438E-5	6.721370367140706E-4
20-21	0.0	0.0	0.0	2.6531725133450157E-5	7.075126702253375E-4
22-23	0.0	0.0	0.0	7.959517540035047E-5	7.075126702253375E-4
24-25	0.0	0.0	0.0	2.741611597123183E-4	7.252004869809709E-4
26-27	0.0	0.0	0.0	5.394784110468199E-4	7.51732212114421E-4
28-29	0.0	0.0	0.0	0.00283005068090135	7.605761204922378E-4
30-31	0.0	0.0	0.0	0.011788929867629687	7.605761204922378E-4
32-33	0.0	0.0	0.0	0.028680794869259618	7.782639372478712E-4
34-35	0.0	0.0	0.0	0.05500026620164217	8.49015204270405E-4
36-37	0.0	0.0	0.0	0.09933477889963738	8.49015204270405E-4
38-39	0.0	0.0	0.0	0.16513345723059375	8.843908377816718E-4
40-41	0.0	0.0	0.0	0.23665414428199757	8.932347461594885E-4
42-43	0.0	0.0	0.0	0.3133131421009129	9.020786545373053E-4
44-45	0.0	0.0	0.0	0.40623608742663314	9.286103796707554E-4
46-47	0.0	0.0	0.0	0.5229137706551692	9.639860131820223E-4
48-49	0.0	0.0	0.0	0.6559173087491548	0.001008205555071106
50-51	0.0	0.0	0.0	0.7974640623361113	0.001008205555071106
52-53	0.0	0.0	0.0	0.938188332443931	0.0011762398142496236
54-55	0.0	0.0	0.0	1.0929036656054567	0.0012823667147834241
56-57	0.0	0.0	0.0	1.282234056157757	0.0012912106231612408
58-59	0.0	0.0	0.0	1.512494054682593	0.0013000545315390577
60-61	0.0	0.0	0.0	1.7453276305453738	0.0013442740734281413
62-63	0.0	0.0	0.0	1.993629202160956	0.0013973375236950416
64-65	0.0	0.0	0.0	2.25371085973579	0.0013973375236950416
66-67	0.0	0.0	0.0	2.550317858911007	0.001415025340450675
68-69	0.0	0.0	0.0	2.88862388608763	0.0014327131572063085
70-71	0.0	0.0	0.0	3.2547970685627536	0.0014680887907175752
72-73	0.0	0.0	0.0	3.6365885932331015	0.0015919035080070093
74-75	0.0	0.0	0.0	4.0147983350104335	0.0016095913247626428
76-77	0.0	0.0	0.0	4.446071527054666	0.0016184352331404595
78-79	0.0	0.0	0.0	4.911393766353492	0.0016361230498960929
80-81	0.0	0.0	0.0	5.417362608556765	0.0016803425917851765
82-83	0.0	0.0	0.0	5.946228329550204	0.0017864694923189772
84-85	0.0	0.0	0.0	6.462500325013632	0.0017864694923189772
86-87	0.0	0.0	0.0	7.006321095073961	0.0018483768509636941
88	0.0	0.0	0.0	7.445491897299584	0.0019279720263640446
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5670	0.0	42.305824	94
GTACTAG	6455	0.0	37.368473	1
GCATCGT	6860	0.0	32.058044	8
CTAACGC	3150	0.0	31.926914	3
CAACGCA	9945	0.0	31.611721	5
CTGTGCG	8330	0.0	31.533941	9
TCTAACG	3220	0.0	31.3788	2
CGCCTAC	3205	0.0	30.936398	7
TAGGCAT	8345	0.0	30.858944	5
TAGTACT	8915	0.0	30.310728	4
TAACGCC	3325	0.0	29.963608	4
ATCAACG	10500	0.0	29.897913	3
GTATCAA	11765	0.0	29.69485	1
TATTCTC	3155	0.0	29.044378	5
ACTGTGC	9590	0.0	28.81204	8
CTAGGCA	9340	0.0	28.73023	4
GTACTGT	9765	0.0	27.718235	6
CTAGTAC	10205	0.0	27.538605	3
TACTGTG	10480	0.0	27.396507	7
ACGCCTA	3640	0.0	27.368425	6
>>END_MODULE