FastQCFastQC Report
Mon 16 Jan 2017
ERR1146668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5653609
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133150.2355132801012592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113330.20045602729159376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74810.13232255714889374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59650.044.30848394
GGTATCA116450.042.612731
GTATCAA189900.036.8804441
CTGTGCG85750.032.8359839
GTACTAG63500.032.3693581
TAGTACT92200.031.0163964
ACTGTGC96350.030.2922768
CAACGCA221700.030.188145
ATCAACG226050.029.6294883
TACTGTG107200.028.7156967
GTACTGT99200.028.5205366
CTAGTAC105400.028.4261133
TATTCGC6050.027.9707729
TATCAAC251450.027.871542
AACGCAG259050.026.9229816
CTAACGC26650.026.6500723
TCTAACG27700.026.1497362
CGCCTAC27650.026.0057357
TATTCTC34850.025.7586235
TAACGCC28200.025.6856354
Produced by FastQC (version 0.11.5)