FastQCFastQC Report
Mon 16 Jan 2017
ERR1146667_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146667_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA65760.10227618957115699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC69100.041.86147394
GTACTAG72450.033.9447251
CTGTGCG95100.032.563179
TAGTACT97050.032.4455454
TAGGCAT89850.031.015255
CAACGCA113550.030.7910485
ACTGTGC105500.030.2444618
GTACTGT105850.029.7007336
GTATCAA135100.029.5502591
ATCAACG119600.029.4719773
CTAGGCA100000.029.1855184
GCATCGT76650.028.9986348
CTAGTAC113500.028.9027233
TCTAACG40400.028.6180292
CTAACGC40600.028.2450923
TACTGTG119100.028.0931327
CGCCTAC41500.027.6295417
TAACGCC42700.027.0759124
TATTCTC34900.026.6611235
TACATGG265800.026.187022
Produced by FastQC (version 0.11.5)