FastQCFastQC Report
Mon 16 Jan 2017
ERR1146667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429649
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139880.21755464411820927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117250.18235832158178464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.11446970122319274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC68550.042.46671794
GGTATCA121050.041.194741
GTATCAA207350.038.1839451
CTGTGCG92100.033.428549
TAGTACT95750.032.5720444
GTACTAG70200.032.5016441
ATCAACG238950.031.8917433
CAACGCA238900.031.8517045
ACTGTGC103750.030.6224738
GTACTGT106650.029.4806356
TATCAAC268500.029.4701522
GTGGTAT28800.029.0755671
TACTGTG119650.028.7523487
TAGGCAT90000.028.2002665
AACGCAG284700.027.9473766
CTAGTAC115300.027.9059663
TACCCCG4850.027.1342755
ACGCAGA288800.026.3140537
CTAGGCA101750.026.3055934
CGCAGAG291600.026.045678
Produced by FastQC (version 0.11.5)