FastQCFastQC Report
Mon 16 Jan 2017
ERR1146666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572044
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58450.044.33600694
GTACTAG65800.036.3704831
CAACGCA89050.032.8236965
TAGGCAT81950.032.5136075
GCATCGT71200.031.2825498
CTGTGCG87400.031.236389
GTATCAA109750.030.7593441
TAGTACT93900.030.279464
CTAGGCA92000.030.0363964
ATCAACG98050.029.9090843
ACTGTGC98100.029.314758
GTACTGT101950.027.7473076
TACTGTG107200.027.5714537
CTAGTAC107600.027.3855483
CGTTAAC10500.026.8669971
GAGTTGC71950.026.71823594
TATCAAC126050.026.6958732
ACATGGG211150.026.6198883
AGGGCTA66400.026.6103655
GTACATG219900.026.5768241
Produced by FastQC (version 0.11.5)