##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146666_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5572044
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27062115805259	31.0	31.0	34.0	28.0	34.0
2	31.39677594074993	31.0	31.0	34.0	28.0	34.0
3	31.531894758907143	33.0	31.0	34.0	28.0	34.0
4	34.96514331186186	37.0	35.0	37.0	32.0	37.0
5	35.12341108577032	37.0	35.0	37.0	32.0	37.0
6	35.20017645230368	37.0	35.0	37.0	32.0	37.0
7	35.125480164908964	37.0	35.0	37.0	32.0	37.0
8	35.10551962619103	37.0	35.0	37.0	32.0	37.0
9	36.59534831383241	39.0	37.0	39.0	32.0	39.0
10-11	36.42630110243207	39.0	37.0	39.0	31.5	39.0
12-13	36.34725506474823	39.0	37.0	39.0	31.0	39.0
14-15	37.50475337237107	40.0	37.0	41.0	31.0	41.0
16-17	37.53229470190831	40.0	37.0	41.0	31.0	41.0
18-19	37.49974129780741	40.0	37.0	41.0	31.0	41.0
20-21	37.56331321145346	40.0	37.0	41.0	31.0	41.0
22-23	37.49652551200242	40.0	37.0	41.0	31.0	41.0
24-25	37.456370678336356	40.0	37.0	41.0	31.0	41.0
26-27	37.49073212630769	40.0	37.0	41.0	31.0	41.0
28-29	37.47321727897339	40.0	37.0	41.0	31.0	41.0
30-31	37.33546468764425	40.0	36.0	41.0	30.0	41.0
32-33	37.32253980765407	40.0	36.0	41.0	30.0	41.0
34-35	37.25657056907663	40.0	36.0	41.0	30.0	41.0
36-37	37.145462329443205	40.0	36.0	41.0	30.0	41.0
38-39	37.038044835970425	40.0	36.0	41.0	30.0	41.0
40-41	36.95188363552047	40.0	36.0	41.0	30.0	41.0
42-43	36.791740966151735	39.5	35.0	41.0	30.0	41.0
44-45	36.641874956479164	39.0	35.0	41.0	29.5	41.0
46-47	36.63717255283699	39.0	35.0	41.0	29.5	41.0
48-49	36.49553224992481	39.0	35.0	41.0	29.0	41.0
50-51	36.3159120782248	39.0	35.0	41.0	29.0	41.0
52-53	36.097412906287175	38.5	35.0	40.5	28.0	41.0
54-55	35.877386108221685	38.0	34.5	40.0	28.0	41.0
56-57	35.63397309856132	38.0	34.0	40.0	28.0	41.0
58-59	35.32984053966552	37.0	34.0	40.0	28.0	41.0
60-61	35.07901104154956	37.0	34.0	40.0	27.0	41.0
62-63	34.77455795395729	36.0	33.0	40.0	27.0	41.0
64-65	34.62174177016549	36.0	33.0	39.0	27.0	41.0
66-67	34.367131612743904	35.0	33.0	39.0	26.5	41.0
68-69	34.24279860316968	35.0	33.0	39.0	26.0	41.0
70-71	34.16621602413764	35.0	33.0	38.5	27.0	41.0
72-73	33.951216555361015	35.0	33.0	37.5	26.0	40.0
74-75	33.69618653406182	35.0	33.0	37.0	26.0	39.5
76-77	33.437737300710474	35.0	33.0	36.5	26.0	39.0
78-79	33.18414974827909	35.0	33.0	36.0	26.0	39.0
80-81	32.999646987712225	35.0	33.0	36.0	26.0	38.5
82-83	32.73367636723616	35.0	33.0	35.5	25.5	37.0
84-85	32.553811132862556	35.0	32.5	35.0	25.0	37.0
86-87	32.375677848200766	35.0	32.0	35.0	25.0	36.5
88-89	32.24546485993291	35.0	32.0	35.0	25.0	36.0
90-91	32.08995720421447	35.0	32.0	35.0	25.0	36.0
92-93	31.92925944231596	35.0	32.0	35.0	24.5	36.0
94-95	31.858503809374085	35.0	32.0	35.0	24.0	35.5
96-97	31.745654108259018	35.0	32.0	35.0	24.0	35.0
98-99	31.648744966838024	35.0	32.0	35.0	24.0	35.0
100	30.68951824501027	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.297753407698238
1101	2	0.39040218863727816
1101	3	0.46616484593978313
1101	4	0.5233197557903608
1101	5	0.43128496100553804
1101	6	0.4433604169946932
1101	7	0.37757356210934745
1101	8	0.40067930988614364
1101	9	0.5383739658480451
1101	10-11	0.5757783656875617
1101	12-13	0.4491974714285689
1101	14-15	0.6402894723179529
1101	16-17	0.6005970883244771
1101	18-19	0.6346225002733092
1101	20-21	0.6384599909033355
1101	22-23	0.6365064977019514
1101	24-25	0.6916968335867182
1101	26-27	0.6030403512111704
1101	28-29	0.6556916898005056
1101	30-31	0.696654259737457
1101	32-33	0.6068711586778903
1101	34-35	0.631300266869971
1101	36-37	0.6339794434375321
1101	38-39	0.5975984854944372
1101	40-41	0.5803868267520684
1101	42-43	0.5726536411525274
1101	44-45	0.6058074565623812
1101	46-47	0.5984753412042423
1101	48-49	0.5192556586934316
1101	50-51	0.5264129124460837
1101	52-53	0.5421323074764359
1101	54-55	0.5165691662130243
1101	56-57	0.5190991230922677
1101	58-59	0.5004557154777416
1101	60-61	0.5207040066528492
1101	62-63	0.5378332253481446
1101	64-65	0.5797277078254837
1101	66-67	0.5488544652173317
1101	68-69	0.5996466414556565
1101	70-71	0.5136558562058724
1101	72-73	0.47883623655374663
1101	74-75	0.4798852763811823
1101	76-77	0.4910148633422793
1101	78-79	0.5079957866264522
1101	80-81	0.44688334934728857
1101	82-83	0.4378449810898104
1101	84-85	0.4315091823386439
1101	86-87	0.46154312561002087
1101	88-89	0.48519353061035986
1101	90-91	0.48204694236498113
1101	92-93	0.4704339327882714
1101	94-95	0.4261720646758711
1101	96-97	0.464420020297716
1101	98-99	0.4717031699152159
1101	100	0.6155213155836847
1102	1	0.31356724679995196
1102	2	0.3610148384637206
1102	3	0.3667950069868411
1102	4	0.48891586218146443
1102	5	0.3526574551058417
1102	6	0.3740110495467377
1102	7	0.33853173784549284
1102	8	0.4271166987331583
1102	9	0.49159843662130953
1102	10-11	0.6161657151020705
1102	12-13	0.5185578450619985
1102	14-15	0.6649694010585918
1102	16-17	0.5920552257287355
1102	18-19	0.6419575536077673
1102	20-21	0.589898043916719
1102	22-23	0.6398732898391302
1102	24-25	0.7085569054988525
1102	26-27	0.6768305626893323
1102	28-29	0.6087682602123863
1102	30-31	0.6065389908184002
1102	32-33	0.569633969308498
1102	34-35	0.6453827950439148
1102	36-37	0.7091629241398962
1102	38-39	0.670970537829227
1102	40-41	0.6167061002527419
1102	42-43	0.6643834795285883
1102	44-45	0.6649294969421717
1102	46-47	0.6519306762059642
1102	48-49	0.6033060260343532
1102	50-51	0.5892342456215331
1102	52-53	0.5972123831686318
1102	54-55	0.5392222869220333
1102	56-57	0.5284733948812885
1102	58-59	0.42871997930436123
1102	60-61	0.483501885267529
1102	62-63	0.5015300056973189
1102	64-65	0.4935999419520982
1102	66-67	0.4973575860401951
1102	68-69	0.48242719861153205
1102	70-71	0.46814076293034645
1102	72-73	0.43727441329183137
1102	74-75	0.4029007283065411
1102	76-77	0.4102562183631093
1102	78-79	0.4323471238020815
1102	80-81	0.4457624642374469
1102	82-83	0.4563590929172108
1102	84-85	0.4420065404746083
1102	86-87	0.393954747838265
1102	88-89	0.4846145673019393
1102	90-91	0.4380281969334945
1102	92-93	0.4840932162021012
1102	94-95	0.5118862636419017
1102	96-97	0.5184663115547821
1102	98-99	0.42476152319251526
1102	100	0.39946344278331125
1103	1	0.29194034571959904
1103	2	0.29934498689065236
1103	3	0.3495284400414782
1103	4	0.4316617661173652
1103	5	0.2658268572468785
1103	6	0.35874552727354825
1103	7	0.3351557227637869
1103	8	0.36176293396794534
1103	9	0.4269952548026126
1103	10-11	0.5896926958908537
1103	12-13	0.47891382267959415
1103	14-15	0.6084711341737332
1103	16-17	0.5757794534318137
1103	18-19	0.6011444647288684
1103	20-21	0.5252178411119459
1103	22-23	0.6330010466129181
1103	24-25	0.6364813871939319
1103	26-27	0.5167125310301017
1103	28-29	0.45126771271725374
1103	30-31	0.5670673221904607
1103	32-33	0.5512593179614029
1103	34-35	0.5739215362568331
1103	36-37	0.5633768855230485
1103	38-39	0.6271914119459439
1103	40-41	0.5371029384469139
1103	42-43	0.4994799901977416
1103	44-45	0.5039363093254607
1103	46-47	0.4627903350493128
1103	48-49	0.4579772173265795
1103	50-51	0.4380756072633645
1103	52-53	0.4776998925603877
1103	54-55	0.4523410707795321
1103	56-57	0.4762750018009356
1103	58-59	0.5189451684832278
1103	60-61	0.5036000956017261
1103	62-63	0.4668389130285533
1103	64-65	0.47192400217598873
1103	66-67	0.535121477263921
1103	68-69	0.46038046311200276
1103	70-71	0.4420418701570554
1103	72-73	0.47146296118035025
1103	74-75	0.43739926136267115
1103	76-77	0.3449618775472487
1103	78-79	0.4363490987871259
1103	80-81	0.3941841915888773
1103	82-83	0.4227288971455323
1103	84-85	0.45516821621568226
1103	86-87	0.4438355211243987
1103	88-89	0.494517424797678
1103	90-91	0.5021213648804164
1103	92-93	0.4226227143248984
1103	94-95	0.36947965659073745
1103	96-97	0.3867885934916089
1103	98-99	0.3750823796407161
1103	100	0.4181426472314307
1104	1	0.21968288194399932
1104	2	0.33554132381462054
1104	3	0.3256514301316855
1104	4	0.47416049764595414
1104	5	0.3642893688651796
1104	6	0.33756806026503483
1104	7	0.33292349371152596
1104	8	0.3814161585794338
1104	9	0.461935748900288
1104	10-11	0.5862289270265464
1104	12-13	0.496254949441429
1104	14-15	0.6039527940976654
1104	16-17	0.6155426084906637
1104	18-19	0.5930732821542932
1104	20-21	0.5500651193988588
1104	22-23	0.5658432027449081
1104	24-25	0.6019294606889432
1104	26-27	0.541589304083729
1104	28-29	0.5392800123998001
1104	30-31	0.43430927244842366
1104	32-33	0.542423348153271
1104	34-35	0.5657668085628416
1104	36-37	0.5406579170484918
1104	38-39	0.5471079285662839
1104	40-41	0.5461857550371718
1104	42-43	0.5951610695434368
1104	44-45	0.5388118644220157
1104	46-47	0.5424450077387846
1104	48-49	0.5413013645321243
1104	50-51	0.44196939354950615
1104	52-53	0.45555437419336897
1104	54-55	0.5691055481917644
1104	56-57	0.5024832208221781
1104	58-59	0.5196289363147883
1104	60-61	0.49553129842693266
1104	62-63	0.5925895562445831
1104	64-65	0.5352791820295195
1104	66-67	0.515510825022659
1104	68-69	0.4894838278166205
1104	70-71	0.43329452496669774
1104	72-73	0.42700491839806887
1104	74-75	0.4539724858394507
1104	76-77	0.38188040127103307
1104	78-79	0.4152952796778848
1104	80-81	0.45339172249242665
1104	82-83	0.4254345017686063
1104	84-85	0.40990396443420707
1104	86-87	0.3807354948569355
1104	88-89	0.3563182500898705
1104	90-91	0.3949070740345135
1104	92-93	0.4040010207696305
1104	94-95	0.46872713311840286
1104	96-97	0.46940680267938006
1104	98-99	0.4655494521434065
1104	100	0.4670075898246786
1105	1	0.08712459963088293
1105	2	0.16849664437982526
1105	3	0.20412338511913575
1105	4	0.26159583499040195
1105	5	0.16442978339943437
1105	6	0.1686223207886286
1105	7	0.23623994988368935
1105	8	0.2457023386754713
1105	9	0.3358365939137826
1105	10-11	0.36730398535145525
1105	12-13	0.1922782365162945
1105	14-15	0.42132727143540905
1105	16-17	0.3541213108371082
1105	18-19	0.3639931677311381
1105	20-21	0.3001119438272255
1105	22-23	0.35571960857934215
1105	24-25	0.351714535387714
1105	26-27	0.36915614345831926
1105	28-29	0.45873299203734064
1105	30-31	0.4183843842366102
1105	32-33	0.40143309961954543
1105	34-35	0.3625136564328031
1105	36-37	0.4077346303848728
1105	38-39	0.3607699102442439
1105	40-41	0.35350116133162146
1105	42-43	0.38464321310348737
1105	44-45	0.3411536145981202
1105	46-47	0.4000154661658044
1105	48-49	0.2636254835778047
1105	50-51	0.33182290123969693
1105	52-53	0.36469630038702405
1105	54-55	0.32419395941011686
1105	56-57	0.3342435196995055
1105	58-59	0.39329103255690256
1105	60-61	0.44064885300500123
1105	62-63	0.3352959465159202
1105	64-65	0.4127879315000271
1105	66-67	0.3124751538558712
1105	68-69	0.297337788475879
1105	70-71	0.3275884366747448
1105	72-73	0.3560691260282738
1105	74-75	0.26581087047305374
1105	76-77	0.2576527252725711
1105	78-79	0.28878413434767225
1105	80-81	0.23454621196809455
1105	82-83	0.2603053203970802
1105	84-85	0.24719301215928624
1105	86-87	0.24807281228132183
1105	88-89	0.23968357815756747
1105	90-91	0.21225628850626066
1105	92-93	0.20143968928734424
1105	94-95	0.22464992392483296
1105	96-97	0.19601253687996234
1105	98-99	0.16832766239272345
1105	100	0.25981451749004236
1106	1	-0.004118362195910663
1106	2	0.0774607219620762
1106	3	0.05768288718518022
1106	4	0.12322520719632735
1106	5	0.015761660110996445
1106	6	0.08769736538694417
1106	7	0.11447150895813252
1106	8	0.07972722454164227
1106	9	0.11904078040215893
1106	10-11	0.15368196470098638
1106	12-13	0.035865681268575145
1106	14-15	0.10511847672855623
1106	16-17	0.059592152215650174
1106	18-19	0.09436027478938058
1106	20-21	0.15392070267450464
1106	22-23	0.1260717524526953
1106	24-25	0.1732911011653755
1106	26-27	0.2353892977761589
1106	28-29	0.22885555464306861
1106	30-31	0.21484589147083
1106	32-33	0.14755683618798088
1106	34-35	0.19238039760715253
1106	36-37	0.21330868034708317
1106	38-39	0.23025283717019107
1106	40-41	0.23408525502416921
1106	42-43	0.1678713674210428
1106	44-45	0.18170041469415565
1106	46-47	0.14439022817995095
1106	48-49	0.10328803786047303
1106	50-51	0.09263521533417673
1106	52-53	0.09289207192037452
1106	54-55	0.15088536652276474
1106	56-57	0.12050786791657231
1106	58-59	0.11922894527817363
1106	60-61	0.1334589334821743
1106	62-63	0.1123477468138887
1106	64-65	0.09373706214480393
1106	66-67	0.09570330817342665
1106	68-69	0.09295055512446027
1106	70-71	0.058382485094021774
1106	72-73	0.10382716894901023
1106	74-75	0.11850359846659586
1106	76-77	0.10929762413784516
1106	78-79	0.0619428908529116
1106	80-81	0.06098229832349489
1106	82-83	0.07035742219422048
1106	84-85	0.09046841421242391
1106	86-87	0.08783280788875913
1106	88-89	0.14605565888605554
1106	90-91	0.0908038080980873
1106	92-93	0.15173762784086264
1106	94-95	0.12746311330601046
1106	96-97	0.07129685957474408
1106	98-99	0.08918780613402078
1106	100	0.15840686268393966
1107	1	0.13359066246832185
1107	2	0.21358021838685204
1107	3	0.15728202828794124
1107	4	0.22481396041320778
1107	5	0.17614155895332573
1107	6	0.22611885107937724
1107	7	0.20122981773832294
1107	8	0.21294921296539115
1107	9	0.20548468835875866
1107	10-11	0.29395091045069677
1107	12-13	0.1648707718751794
1107	14-15	0.36006329296855455
1107	16-17	0.25193671605662615
1107	18-19	0.1983611350644381
1107	20-21	0.2145409661563562
1107	22-23	0.3058005849715997
1107	24-25	0.34679589963151614
1107	26-27	0.2164125471616316
1107	28-29	0.2901636506580303
1107	30-31	0.371578749370876
1107	32-33	0.36426916460121106
1107	34-35	0.33197670987753014
1107	36-37	0.3503664492379386
1107	38-39	0.3788837813242125
1107	40-41	0.30577230263165234
1107	42-43	0.28150263665398256
1107	44-45	0.3502943687430715
1107	46-47	0.31567396265204906
1107	48-49	0.32011431612026797
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2208	96-97	0.07571222287294432
2208	98-99	0.016835187029794696
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2215	26-27	-1.0393519753927833
2215	28-29	-0.8903594881175678
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2216	2	-0.7620853881097105
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2216	5	-0.5439866720469055
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2216	30-31	-1.5060928113845833
2216	32-33	-1.6657682303173615
2216	34-35	-1.3195359352642413
2216	36-37	-1.4662112383027477
2216	38-39	-1.33033600359105
2216	40-41	-1.1948986331382088
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2216	52-53	-1.340775116377884
2216	54-55	-1.383902714822149
2216	56-57	-1.3136406744699656
2216	58-59	-1.2300220435712816
2216	60-61	-1.3038305916324902
2216	62-63	-1.3778040600384287
2216	64-65	-1.368816744171916
2216	66-67	-1.3336029432988425
2216	68-69	-1.3569497570068023
2216	70-71	-1.2319957640744335
2216	72-73	-1.1636921904601678
2216	74-75	-1.2073728745397219
2216	76-77	-1.2053188059081847
2216	78-79	-1.0551292909485355
2216	80-81	-1.1292510783856144
2216	82-83	-1.1404159178347193
2216	84-85	-1.1862816812727885
2216	86-87	-1.1951103165973294
2216	88-89	-1.1782216533155285
2216	90-91	-1.2500781397117748
2216	92-93	-1.1904116137158844
2216	94-95	-1.1556412704410128
2216	96-97	-1.180166837003533
2216	98-99	-1.2043651783950509
2216	100	-0.8567622413478375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	2.0
2	23.0
3	22.0
4	57.0
5	31.0
6	48.0
7	166.0
8	557.0
9	920.0
10	1504.0
11	2604.0
12	4669.0
13	7347.0
14	10245.0
15	13654.0
16	16598.0
17	19841.0
18	22893.0
19	25754.0
20	29480.0
21	33846.0
22	39471.0
23	44875.0
24	52615.0
25	60049.0
26	67357.0
27	74774.0
28	85131.0
29	102265.0
30	119498.0
31	139140.0
32	171560.0
33	220353.0
34	315588.0
35	452244.0
36	719600.0
37	1236940.0
38	1066813.0
39	412795.0
40	715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73826012439689	20.637740570926987	11.408483382262203	24.215515922413925
2	15.841899049290781	20.06183109790133	39.94888389932569	24.147385953482196
3	18.628361937619005	27.18701500699709	28.852066039904866	25.332557015479036
4	11.976986593461229	16.53994814714437	40.9171978637844	30.56586739561001
5	12.778935281361312	38.97032892265541	35.52146079843757	12.7292749975457
6	29.301358853763045	39.08927328585463	18.380653500366662	13.228714360015664
7	26.324265128689973	31.920590036317325	24.433237209789812	17.32190762520289
8	26.16175279484891	34.883389953278446	20.286930223851734	18.667927028020905
9	26.306890662677198	14.604996694187586	21.641477644308942	37.44663499882627
10-11	25.55461573927259	25.867276643926225	28.002448308160194	20.575659308640994
12-13	25.674532537584405	23.87805834700505	27.83936469781052	22.608044417600027
14-15	23.087972782603522	25.091445667548662	25.12616374892106	26.694417800926757
16-17	22.012534087819375	27.546942258666313	26.518380726506884	23.92214292700742
18-19	21.27026100327866	28.510959779459277	28.06656351291556	22.1522157043465
20-21	23.07796952073633	27.610950137159378	27.03108497241109	22.279995369693207
22-23	23.577140638899078	27.254669371751543	26.633381643548145	22.53480834580123
24-25	23.03464140978429	27.291993338804765	27.573563260267054	22.09980199114389
26-27	22.952077163557615	27.47338273281797	26.441948486437095	23.132591617187316
28-29	21.99253118251491	28.127936365639133	26.962834938056734	22.916697513789224
30-31	22.85801639445181	27.77750956025947	27.03277221231413	22.33170183297459
32-33	23.35663197296774	27.85003463028336	26.004776268985857	22.788557127763042
34-35	23.247096111876868	27.546923457960027	27.140756015966	22.065224414197104
36-37	22.878058174849052	27.18800025055716	26.77780264603435	23.156138928559443
38-39	23.057635316263223	27.268100798178217	27.414411934049433	22.25985195150913
40-41	22.676551002326665	26.34927346830503	28.064006903444298	22.91016862592401
42-43	22.585634204805913	26.68206479328975	27.290214809430687	23.442086192473653
44-45	22.72590116522233	27.704527806189027	27.25682172209643	22.31274930649221
46-47	22.78282176961768	27.380868507573858	26.69119470432762	23.14511501848084
48-49	23.48046644482789	26.98855877343948	26.951463978872216	22.57951080286042
50-51	23.02759856453898	26.688445992731154	27.002885012787125	23.281070429942744
52-53	22.39867921735694	27.478134679941867	26.747814186044987	23.375371916656203
54-55	22.42626198331961	27.292279104565754	26.754473590161	23.526985321953635
56-57	23.62545085667817	26.6641591276708	26.519890375492743	23.190499640158283
58-59	22.413490432480977	27.118387446332303	27.53882589202937	22.92929622915735
60-61	22.472068331430524	27.82725547847878	26.655089743294035	23.045586446796666
62-63	22.88345950525291	27.124271899551864	26.2333340662388	23.75893452895642
64-65	21.99251750546182	27.40768754159634	26.75427055729579	23.845524395646052
66-67	22.8053514156144	26.84733571238246	26.722705320426286	23.62460755157685
68-69	22.153734795269237	26.924969370006107	27.099627414717244	23.821668420007413
70-71	22.22654976439674	26.598951799486265	27.136719710866736	24.037778725250263
72-73	22.84287068863553	26.646116552512993	27.71883681890766	22.792175939943817
74-75	23.00262078631846	26.95804902143879	27.115558252255102	22.923771939987645
76-77	22.763084907219035	26.904868293652157	26.769831112433735	23.562215686695072
78-79	22.57409034109001	26.839250066500654	26.823481963336153	23.763177629073187
80-81	22.096246884818374	27.24517881613675	27.55968091206954	23.098893386975334
82-83	22.990579243825007	26.137418176704664	28.687229620690253	22.184772958780073
84-85	23.2504903112487	26.717552182452682	27.20600574965884	22.825951756639775
86-87	21.780858392292906	27.486467618892323	27.704899010480666	23.0277749783341
88-89	23.129107321857884	26.09718384558848	27.39759010195446	23.37611873059917
90-91	22.723411633540277	26.14643746039421	27.575077223710352	23.555073682355157
92-93	23.094996285158736	26.433489444313775	27.480088423901307	22.99142584662619
94-95	21.894690139373836	26.505195782781136	28.150790234965186	23.449323842879842
96-97	22.288902537653062	26.35226866032753	27.674805789293632	23.684023012725778
98-99	21.60907426116913	27.113810026278195	27.518823793569425	23.758291918983247
100	22.533001631638385	26.284042547831767	26.595993525267364	24.58696229526249
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	782.0
1	527.0
2	308.5
3	347.5
4	323.0
5	267.0
6	243.0
7	255.0
8	276.5
9	283.0
10	250.0
11	229.5
12	259.0
13	286.0
14	325.0
15	514.5
16	652.5
17	763.5
18	1058.0
19	1372.5
20	1787.0
21	2353.0
22	3415.5
23	4960.5
24	6627.5
25	8649.0
26	12142.0
27	16016.0
28	21433.0
29	32925.5
30	49219.5
31	64320.5
32	84143.0
33	103752.0
34	118267.5
35	135202.5
36	150302.0
37	175465.0
38	184417.0
39	169958.0
40	157393.0
41	144883.0
42	143212.0
43	153151.0
44	168503.0
45	201293.0
46	234155.0
47	257424.5
48	270815.0
49	263686.0
50	250040.5
51	255742.5
52	259027.5
53	245498.5
54	229807.0
55	195457.0
56	169114.5
57	136902.5
58	104058.0
59	101688.5
60	84525.0
61	56196.5
62	41267.0
63	34572.5
64	25005.0
65	11527.5
66	6117.5
67	4094.5
68	2959.5
69	2221.5
70	1783.0
71	1407.0
72	1048.0
73	880.0
74	668.5
75	477.5
76	342.5
77	217.5
78	141.5
79	95.5
80	66.5
81	43.0
82	33.0
83	32.5
84	26.0
85	18.0
86	16.0
87	11.5
88	9.0
89	12.0
90	10.5
91	7.5
92	9.5
93	9.0
94	6.5
95	6.0
96	6.5
97	7.5
98	6.0
99	4.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06130604855238042
2	0.007429948507226432
3	0.007950403837442777
4	0.009134888382073077
5	0.003356039543119186
6	0.002548436444507617
7	8.614433051856733E-4
8	0.0017408333458960484
9	7.178694209880611E-4
10-11	5.922422723151504E-4
12-13	3.3201460720697827E-4
14-15	3.499613427316798E-4
16-17	6.999226854633596E-4
18-19	0.0018395403912819068
20-21	0.0010588573959573902
22-23	0.0012293513834420546
24-25	0.0012742182222538086
26-27	0.0034278264852179922
28-29	0.003795734563474373
30-31	0.005043032682441129
32-33	0.0059403694586762056
34-35	0.0036611340470391117
36-37	0.008273445076887405
38-39	0.006389037846793744
40-41	0.005967289561963258
42-43	0.014420201994097678
44-45	0.019561941721924665
46-47	0.018027495834562687
48-49	0.021204068022434856
50-51	0.018153122983235598
52-53	0.025260030251017397
54-55	0.019220953746955337
56-57	0.015156018150610441
58-59	0.01713913242608996
60-61	0.01621487554656783
62-63	0.010974428773354986
64-65	0.011485910735808978
66-67	0.013827959721782527
68-69	0.005375047289648108
70-71	0.008892607452489607
72-73	0.008273445076887405
74-75	0.011207736335176103
76-77	0.01104621571545379
78-79	0.012024312801550023
80-81	0.009673290447814124
82-83	0.006415957950080795
84-85	0.010462946810900991
86-87	0.008982341130113114
88-89	0.00787861689534397
90-91	0.013110090300794467
92-93	0.008497779270946174
94-95	0.007358161565127627
96-97	0.009296409001795392
98-99	0.009063101439974271
100	0.2591508609766901
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5572044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.22818797962141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14713494377614	49.106303912067624
2	15.070607248568448	19.961980196504726
3	5.526759746840772	10.980818502959263
4	2.3542489207391015	6.236705602941202
5	1.145200011900707	3.7922260831212347
6	0.6528840869157418	2.5943598022295546
7	0.35425606029278495	1.642321586779346
8	0.228044241286474	1.2082365503672592
9	0.15105408855874994	0.9003634714943344
>10	0.36759749396390706	3.3018682548660703
>50	0.0018138043982772758	0.08040109275431469
>100	3.207820596914924E-4	0.04668488070113388
>500	0.0	0.0
>1k	7.857069927082479E-5	0.14773006321393303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.17869420988061E-5
2	0.0	0.0	0.0	0.0	7.17869420988061E-5
3	0.0	0.0	0.0	0.0	7.17869420988061E-5
4	0.0	0.0	0.0	0.0	7.17869420988061E-5
5	0.0	0.0	0.0	0.0	7.17869420988061E-5
6	1.7946735524701526E-5	0.0	0.0	0.0	1.256271486729107E-4
7	1.7946735524701526E-5	0.0	0.0	0.0	1.256271486729107E-4
8	1.7946735524701526E-5	0.0	0.0	0.0	1.256271486729107E-4
9	1.7946735524701526E-5	0.0	0.0	0.0	1.256271486729107E-4
10-11	1.7946735524701526E-5	0.0	0.0	0.0	1.256271486729107E-4
12-13	1.7946735524701526E-5	0.0	0.0	8.973367762350763E-6	2.3330756182111985E-4
14-15	1.7946735524701526E-5	0.0	0.0	1.7946735524701526E-5	2.602276651081721E-4
16-17	1.7946735524701526E-5	0.0	0.0	1.7946735524701526E-5	2.961211361575752E-4
18-19	1.7946735524701526E-5	0.0	0.0	2.692010328705229E-5	3.230412394446275E-4
20-21	1.7946735524701526E-5	0.0	0.0	3.589347104940305E-5	3.40987974969329E-4
22-23	3.589347104940305E-5	0.0	0.0	6.281357433645534E-5	3.5893471049403057E-4
24-25	3.589347104940305E-5	0.0	0.0	2.1536082629641833E-4	3.5893471049403057E-4
26-27	3.589347104940305E-5	0.0	0.0	5.204553302163442E-4	3.7688144601873206E-4
28-29	3.589347104940305E-5	0.0	0.0	0.0030240249359122077	3.858548137810828E-4
30-31	3.589347104940305E-5	0.0	0.0	0.01103724234769144	3.948281815434336E-4
32-33	5.384020657410458E-5	0.0	0.0	0.027341851571882778	3.948281815434336E-4
34-35	5.384020657410458E-5	0.0	0.0	0.05332872461165059	4.3072165259283666E-4
36-37	5.384020657410458E-5	0.0	0.0	0.09783662871291038	4.3072165259283666E-4
38-39	5.384020657410458E-5	0.0	0.0	0.16600730360348914	4.666151236422397E-4
40-41	7.17869420988061E-5	0.0	0.0	0.23995682733302176	4.666151236422397E-4
42-43	7.17869420988061E-5	0.0	0.0	0.32008002808305175	4.666151236422397E-4
44-45	8.973367762350764E-5	0.0	0.0	0.4160143028303438	4.8456185916694126E-4
46-47	1.0768041314820917E-4	0.0	0.0	0.5337987280789599	5.025085946916428E-4
48-49	1.0768041314820917E-4	0.0	0.0	0.6709387075909665	5.025085946916428E-4
50-51	1.0768041314820917E-4	0.0	0.0	0.8215297653787371	5.384020657410459E-4
52-53	1.435738841976122E-4	0.0	0.0	0.9651485164151611	5.742955367904488E-4
54-55	1.6152061972231376E-4	0.0	0.0	1.1246142349198966	5.922422723151504E-4
56-57	1.7049398748466454E-4	0.0	0.0	1.3186274193096823	6.460824788892549E-4
58-59	1.7946735524701528E-4	0.0	0.0	1.551800380614367	6.999226854633595E-4
60-61	1.7946735524701528E-4	0.0	0.0	1.795032487180647	7.268427887504119E-4
62-63	2.3330756182111985E-4	0.0	0.0	2.0535641857817346	7.537628920374641E-4
64-65	2.3330756182111985E-4	0.0	0.0	2.324461185159342	7.806829953245165E-4
66-67	2.4228092958347063E-4	0.0	0.0	2.632839941680288	8.165764663739196E-4
68-69	2.512542973458214E-4	0.0	0.0	2.976421219932937	8.255498341362703E-4
70-71	2.512542973458214E-4	0.0	0.0	3.3553844872725342	8.345232018986211E-4
72-73	2.512542973458214E-4	0.0	0.0	3.752482930859842	8.434965696609718E-4
74-75	2.512542973458214E-4	0.0	0.0	4.143685871827286	8.524699374233226E-4
76-77	2.602276651081721E-4	0.0	0.0	4.589159741021428	8.614433051856733E-4
78-79	2.692010328705229E-4	0.0	0.0	5.06968358469531	8.614433051856733E-4
80-81	3.499613427316798E-4	0.0	0.0	5.591592600489156	8.614433051856733E-4
82-83	3.5893471049403057E-4	0.0	0.0	6.136293970399373	8.973367762350764E-4
84-85	3.5893471049403057E-4	0.0	0.0	6.664448450155813	8.973367762350764E-4
86-87	3.679080782563813E-4	0.0	0.0	7.222879072742426	9.332302472844795E-4
88	3.948281815434336E-4	0.0	0.0	7.673701069122929	9.691237183338825E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5845	0.0	44.336006	94
GTACTAG	6580	0.0	36.370483	1
CAACGCA	8905	0.0	32.823696	5
TAGGCAT	8195	0.0	32.513607	5
GCATCGT	7120	0.0	31.282549	8
CTGTGCG	8740	0.0	31.23638	9
GTATCAA	10975	0.0	30.759344	1
TAGTACT	9390	0.0	30.27946	4
CTAGGCA	9200	0.0	30.036396	4
ATCAACG	9805	0.0	29.909084	3
ACTGTGC	9810	0.0	29.31475	8
GTACTGT	10195	0.0	27.747307	6
TACTGTG	10720	0.0	27.571453	7
CTAGTAC	10760	0.0	27.385548	3
CGTTAAC	1050	0.0	26.866997	1
GAGTTGC	7195	0.0	26.718235	94
TATCAAC	12605	0.0	26.695873	2
ACATGGG	21115	0.0	26.619888	3
AGGGCTA	6640	0.0	26.610365	5
GTACATG	21990	0.0	26.576824	1
>>END_MODULE