FastQCFastQC Report
Mon 16 Jan 2017
ERR1146666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572044
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117680.2111971836546876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98520.17681123838935944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.11071341145188372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC58800.044.3876194
GGTATCA102150.041.1723061
GTATCAA175450.038.584551
CTGTGCG81500.033.971969
CAACGCA196550.032.92775
ATCAACG202450.031.9494693
ACTGTGC89700.031.6985998
TAGTACT88800.031.2548774
GTACTAG64850.031.1209871
GTACTGT94450.030.1536566
TATCAAC225500.029.9580762
TACTGTG103700.029.1408067
AACGCAG234200.028.896486
CTAGTAC102850.027.9457423
TAGGCAT80900.027.712165
GTGGTAT24250.027.1595341
CTAACGC30200.026.7916973
CGCAGAG242000.026.6838518
ACGCAGA242100.026.6335247
TAACGCC31000.026.2520454
Produced by FastQC (version 0.11.5)