##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5572044
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11459062419464	33.0	31.0	34.0	30.0	34.0
2	32.19379692622671	33.0	31.0	34.0	30.0	34.0
3	32.35711293737092	34.0	31.0	34.0	30.0	34.0
4	35.96027292677517	37.0	35.0	37.0	35.0	37.0
5	35.868307213654454	37.0	35.0	37.0	35.0	37.0
6	35.90768719701424	37.0	35.0	37.0	35.0	37.0
7	35.83419172569348	37.0	35.0	37.0	35.0	37.0
8	35.84305687464062	37.0	35.0	37.0	35.0	37.0
9	37.55169790475452	39.0	37.0	39.0	35.0	39.0
10-11	37.49144039422517	39.0	37.0	39.0	34.5	39.0
12-13	37.45171655141273	39.0	37.0	39.0	34.0	39.0
14-15	38.67290540060344	40.0	38.0	41.0	34.0	41.0
16-17	38.67362883351244	40.0	38.0	41.0	34.0	41.0
18-19	38.732739009239694	40.0	38.0	41.0	34.0	41.0
20-21	38.73334049408081	40.0	38.0	41.0	34.0	41.0
22-23	38.69112142689469	40.0	38.0	41.0	34.0	41.0
24-25	38.61447630348935	40.0	38.0	41.0	34.0	41.0
26-27	38.47306320624891	40.0	38.0	41.0	34.0	41.0
28-29	38.41772354992172	40.0	38.0	41.0	34.0	41.0
30-31	38.28936320316207	40.0	38.0	41.0	34.0	41.0
32-33	38.14895862272444	40.0	37.0	41.0	33.5	41.0
34-35	38.04759097379705	40.0	37.0	41.0	33.0	41.0
36-37	38.035233031182095	40.0	37.0	41.0	33.0	41.0
38-39	37.935362588665846	40.0	37.0	41.0	33.0	41.0
40-41	37.82166831417699	40.0	37.0	41.0	33.0	41.0
42-43	37.68422458616622	40.0	37.0	41.0	33.0	41.0
44-45	37.476127611339756	40.0	36.0	41.0	32.0	41.0
46-47	37.232920899404235	40.0	36.0	41.0	31.5	41.0
48-49	36.973884538600196	39.5	35.5	41.0	31.0	41.0
50-51	36.76636867907001	39.0	35.0	41.0	31.0	41.0
52-53	36.574254887434485	39.0	35.0	41.0	30.0	41.0
54-55	36.386924259751	39.0	35.0	41.0	30.0	41.0
56-57	36.1547222168382	38.0	35.0	40.5	30.0	41.0
58-59	35.875777093648225	37.5	35.0	40.0	30.0	41.0
60-61	35.692757361571445	37.0	35.0	40.0	30.0	41.0
62-63	35.47618647663228	37.0	35.0	40.0	29.0	41.0
64-65	35.23267816980626	36.0	34.0	39.5	29.0	41.0
66-67	34.98412898390609	36.0	34.0	39.0	29.0	41.0
68-69	34.73789151700883	35.0	34.0	39.0	29.0	41.0
70-71	34.525684470546175	35.0	34.0	38.5	29.0	41.0
72-73	34.29259280795342	35.0	34.0	37.5	29.0	40.0
74-75	34.038028414707426	35.0	34.0	37.0	29.0	39.5
76-77	33.79174643990608	35.0	34.0	36.5	28.5	39.0
78-79	33.57156790219173	35.0	33.5	36.0	28.0	39.0
80-81	33.44342381359515	35.0	33.0	36.0	28.5	38.5
82-83	33.28261693554466	35.0	33.0	36.0	29.0	37.0
84-85	33.15208889233466	35.0	33.0	35.0	29.0	37.0
86-87	33.00958185183032	35.0	33.0	35.0	29.0	36.5
88-89	32.90532675262435	35.0	33.0	35.0	29.0	36.0
90-91	32.839362269931826	35.0	33.0	35.0	29.0	36.0
92-93	32.76782972280908	35.0	33.0	35.0	29.0	36.0
94-95	32.72268399172727	35.0	33.0	35.0	29.0	35.5
96-97	32.63363776380804	35.0	33.0	35.0	27.5	35.0
98-99	32.56118598489172	35.0	33.0	35.0	27.0	35.0
100	31.338277658970387	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3874621569796517
1101	2	0.40654911284747897
1101	3	0.3594402606841527
1101	4	0.2730153428893729
1101	5	0.24775510117453337
1101	6	0.271819049022767
1101	7	0.3373653296518242
1101	8	0.28597990777696936
1101	9	0.35532165279949623
1101	10-11	0.372529153100146
1101	12-13	0.35240829284146
1101	14-15	0.4834350245710297
1101	16-17	0.5123021807930783
1101	18-19	0.47700113656656384
1101	20-21	0.5003817243776112
1101	22-23	0.493618960238301
1101	24-25	0.4757131305650262
1101	26-27	0.5074007154889912
1101	28-29	0.5165288394998271
1101	30-31	0.4973205686110731
1101	32-33	0.580711559534592
1101	34-35	0.5778070459683349
1101	36-37	0.45592503303571164
1101	38-39	0.4777206128463831
1101	40-41	0.4736382262918042
1101	42-43	0.4394249467493765
1101	44-45	0.442937959883416
1101	46-47	0.4495635441590693
1101	48-49	0.38594031465110135
1101	50-51	0.3047976959361378
1101	52-53	0.4206146310461065
1101	54-55	0.40396511055939754
1101	56-57	0.4047444568531944
1101	58-59	0.37837658448705014
1101	60-61	0.4278529757357461
1101	62-63	0.4740332823400948
1101	64-65	0.4726185638681315
1101	66-67	0.44463069907826025
1101	68-69	0.38288361380210034
1101	70-71	0.37420439813518414
1101	72-73	0.40204763353690964
1101	74-75	0.41908718928044664
1101	76-77	0.4141023155819994
1101	78-79	0.42236900850895864
1101	80-81	0.41146041848023174
1101	82-83	0.4080257078621088
1101	84-85	0.3760732882022211
1101	86-87	0.40795372912371874
1101	88-89	0.41167139505878225
1101	90-91	0.3781428886786813
1101	92-93	0.4134325318544896
1101	94-95	0.39988908612438223
1101	96-97	0.3672287490325772
1101	98-99	0.35050182708307176
1101	100	0.6882084274998554
1102	1	0.3451309254482169
1102	2	0.37892730663376284
1102	3	0.302496230553011
1102	4	0.2277967714315281
1102	5	0.2267859647957522
1102	6	0.22636615967136464
1102	7	0.29352549166286934
1102	8	0.2717822321315353
1102	9	0.3263893514668368
1102	10-11	0.32754641763039416
1102	12-13	0.36420316638798056
1102	14-15	0.46838433159881987
1102	16-17	0.4919060871539713
1102	18-19	0.48136421620352365
1102	20-21	0.4612845276150992
1102	22-23	0.513573743792179
1102	24-25	0.484942348419942
1102	26-27	0.48742361594525363
1102	28-29	0.4893628791266522
1102	30-31	0.5323770151616216
1102	32-33	0.599811466095872
1102	34-35	0.5769230595127794
1102	36-37	0.601391471380964
1102	38-39	0.6156322536712793
1102	40-41	0.5453108097319728
1102	42-43	0.5600552444249445
1102	44-45	0.5284288850937315
1102	46-47	0.5420733795141288
1102	48-49	0.5496893174527457
1102	50-51	0.5019028034893367
1102	52-53	0.5780864267470136
1102	54-55	0.5574127412312819
1102	56-57	0.526858843759328
1102	58-59	0.5149516569191306
1102	60-61	0.4852401756179461
1102	62-63	0.48256949003364724
1102	64-65	0.4903378393423239
1102	66-67	0.45691701778247307
1102	68-69	0.5149698704162518
1102	70-71	0.4875857563895565
1102	72-73	0.46184070965948365
1102	74-75	0.4863921767523749
1102	76-77	0.4654083323220348
1102	78-79	0.5248230462988843
1102	80-81	0.4471572732160425
1102	82-83	0.4516728237481331
1102	84-85	0.386024891348022
1102	86-87	0.41829271204591834
1102	88-89	0.4044598766681986
1102	90-91	0.3772767018816978
1102	92-93	0.42390746014350356
1102	94-95	0.4283770032303522
1102	96-97	0.4564654207317318
1102	98-99	0.3911066814940085
1102	100	0.554226336461241
1103	1	0.27376722228835604
1103	2	0.3714691935850283
1103	3	0.24021652944247052
1103	4	0.22333800253222336
1103	5	0.21308138973774504
1103	6	0.24690233037320297
1103	7	0.25986176002754746
1103	8	0.1900550608201641
1103	9	0.3041998598282234
1103	10-11	0.3375559223174065
1103	12-13	0.29107811564104225
1103	14-15	0.39727885173005717
1103	16-17	0.36565039349050465
1103	18-19	0.37912401050545697
1103	20-21	0.39097865509369
1103	22-23	0.36557539599811406
1103	24-25	0.4037665676032631
1103	26-27	0.3994112648553525
1103	28-29	0.4533465194575115
1103	30-31	0.4476623215930715
1103	32-33	0.4628621632873262
1103	34-35	0.43313319815054996
1103	36-37	0.38004728792348885
1103	38-39	0.4036877468057227
1103	40-41	0.4226432676448937
1103	42-43	0.3919884054188145
1103	44-45	0.4597067887620341
1103	46-47	0.4737256311914919
1103	48-49	0.426632474878609
1103	50-51	0.405643717643251
1103	52-53	0.3656507038190071
1103	54-55	0.3799604991029213
1103	56-57	0.3145887631245827
1103	58-59	0.3732564861978602
1103	60-61	0.4594963756071877
1103	62-63	0.42525423318838307
1103	64-65	0.38399404327697795
1103	66-67	0.41247794584247544
1103	68-69	0.3915663690776796
1103	70-71	0.385823050785838
1103	72-73	0.35206635627553595
1103	74-75	0.36432412753899257
1103	76-77	0.3729889456194968
1103	78-79	0.3761233400879149
1103	80-81	0.3150114682581062
1103	82-83	0.30270576694472595
1103	84-85	0.31662149236100845
1103	86-87	0.3133248870339358
1103	88-89	0.32810511211683746
1103	90-91	0.34935493621991043
1103	92-93	0.3164898231382054
1103	94-95	0.30463284699039406
1103	96-97	0.281416374888515
1103	98-99	0.2473570888990011
1103	100	0.4620298288797926
1104	1	0.22598205195397725
1104	2	0.27673615630319404
1104	3	0.20113312913085224
1104	4	0.15122534655514386
1104	5	0.13933307916205706
1104	6	0.17521062729878878
1104	7	0.21315086150077178
1104	8	0.23436562976888098
1104	9	0.19865815518224395
1104	10-11	0.27336169913828456
1104	12-13	0.28154718065412965
1104	14-15	0.352302615924593
1104	16-17	0.28642627142988175
1104	18-19	0.2913867333859841
1104	20-21	0.3277148500835736
1104	22-23	0.34838438104137737
1104	24-25	0.3493295275644641
1104	26-27	0.31667249475625425
1104	28-29	0.3187604115768252
1104	30-31	0.3312374689827209
1104	32-33	0.37767493466162705
1104	34-35	0.3830255464615746
1104	36-37	0.3472938686633711
1104	38-39	0.35664000176623745
1104	40-41	0.442037016887177
1104	42-43	0.4790488183343271
1104	44-45	0.45950953023184127
1104	46-47	0.46234171765410537
1104	48-49	0.4683886735504146
1104	50-51	0.4284170732328292
1104	52-53	0.39232825726318765
1104	54-55	0.4281881470650575
1104	56-57	0.3816160835292379
1104	58-59	0.4190623359839023
1104	60-61	0.3782504728821152
1104	62-63	0.4156284715442595
1104	64-65	0.39755411493999304
1104	66-67	0.39512825970496124
1104	68-69	0.42210291003470957
1104	70-71	0.4154716056264576
1104	72-73	0.34300354897990815
1104	74-75	0.4024597383585018
1104	76-77	0.3997941319837679
1104	78-79	0.3422297839226971
1104	80-81	0.3418231653606085
1104	82-83	0.3332046747122561
1104	84-85	0.34780725494806575
1104	86-87	0.3653627480992796
1104	88-89	0.38955438560825684
1104	90-91	0.37742463704026363
1104	92-93	0.3374306304248478
1104	94-95	0.3497224875078402
1104	96-97	0.30150991623619205
1104	98-99	0.3381068453075855
1104	100	0.49457627962942396
1105	1	0.18220400209447973
1105	2	0.18778886580432186
1105	3	0.13141713662041354
1105	4	0.15639492207436945
1105	5	0.17792193071804263
1105	6	0.10816791601750708
1105	7	0.14241527734399284
1105	8	0.13366103081100533
1105	9	0.11416344481513363
1105	10-11	0.18342924499185642
1105	12-13	0.18863483927408708
1105	14-15	0.28995091041675636
1105	16-17	0.2537781150022127
1105	18-19	0.19614305541820443
1105	20-21	0.27309390470011863
1105	22-23	0.2334871774056424
1105	24-25	0.26520487412104643
1105	26-27	0.24933243072958078
1105	28-29	0.2726630690540759
1105	30-31	0.29434074475392435
1105	32-33	0.2667417537671639
1105	34-35	0.21755118915182692
1105	36-37	0.2570674105700661
1105	38-39	0.2805408908428575
1105	40-41	0.27526915677530184
1105	42-43	0.19975210421068113
1105	44-45	0.2412095360481743
1105	46-47	0.3255282286936776
1105	48-49	0.2503006524377085
1105	50-51	0.23592873674555648
1105	52-53	0.29777876466491904
1105	54-55	0.24637672025950508
1105	56-57	0.1964689890232023
1105	58-59	0.26432529891857826
1105	60-61	0.19081591398644093
1105	62-63	0.24968003892884383
1105	64-65	0.25260098930658614
1105	66-67	0.2856175837940427
1105	68-69	0.20295047037624414
1105	70-71	0.2419864375725851
1105	72-73	0.23242426852509368
1105	74-75	0.2760359996070818
1105	76-77	0.21459642361946862
1105	78-79	0.18040331102417184
1105	80-81	0.08462223509962996
1105	82-83	0.12638165930132317
1105	84-85	0.1369191947072963
1105	86-87	0.15925956280437248
1105	88-89	0.14785049048557397
1105	90-91	0.15850633421135285
1105	92-93	0.13344776334805886
1105	94-95	0.16173295338446536
1105	96-97	0.14913082989417603
1105	98-99	0.15751520367367533
1105	100	0.25755616392549996
1106	1	0.09616001386844175
1106	2	0.18333937659275534
1106	3	0.10923171308208168
1106	4	0.11846591881744928
1106	5	0.053379588062092864
1106	6	0.11315019970107443
1106	7	0.11404114137415178
1106	8	0.05707010421299685
1106	9	0.10185847078191301
1106	10-11	0.1252861433392809
1106	12-13	0.10889876886074745
1106	14-15	0.1359188906644988
1106	16-17	0.0882516342671309
1106	18-19	0.11649309058349644
1106	20-21	0.09392295940144635
1106	22-23	0.15383349640511312
1106	24-25	0.10554299278127388
1106	26-27	0.1771799744793654
1106	28-29	0.11658591937746365
1106	30-31	0.15991923452602919
1106	32-33	0.08383004105202474
1106	34-35	0.1723296281766622
1106	36-37	0.10474082285550423
1106	38-39	0.08002482199781014
1106	40-41	0.06849636102194268
1106	42-43	0.10633591996859337
1106	44-45	0.030823172337179017
1106	46-47	0.04009692098183848
1106	48-49	0.07011754167966444
1106	50-51	0.1047741683880119
1106	52-53	0.040215402165152625
1106	54-55	0.06505735579185057
1106	56-57	0.0044746645590620915
1106	58-59	-0.04839345433486386
1106	60-61	-0.05522886126014015
1106	62-63	0.048414471290847416
1106	64-65	-0.00531690693564002
1106	66-67	0.01793315725402067
1106	68-69	0.011080810312080303
1106	70-71	0.024645283928371953
1106	72-73	-0.005006261499829634
1106	74-75	-0.017223065720173736
1106	76-77	0.0034196688877372594
1106	78-79	0.017976400659989622
1106	80-81	-0.020519014058692164
1106	82-83	-0.07078728203332219
1106	84-85	-0.024905048625171844
1106	86-87	-0.04254876669612173
1106	88-89	-0.014151711097817099
1106	90-91	-0.08448111684401027
1106	92-93	-0.01781823352148848
1106	94-95	-0.023048797349069616
1106	96-97	-0.03563487506909979
1106	98-99	-0.06606216594810377
1106	100	0.11193290559015168
1107	1	0.11504727090424893
1107	2	0.1580439745863913
1107	3	0.17311940714450458
1107	4	0.13299797956371862
1107	5	0.004583348816069588
1107	6	0.037456285393602684
1107	7	0.10881232241813166
1107	8	0.09931163310864122
1107	9	0.14795793287319015
1107	10-11	0.16187575553951206
1107	12-13	0.09634357541295913
1107	14-15	0.1879925496709305
1107	16-17	0.12243378954462969
1107	18-19	0.1421092012360603
1107	20-21	0.18027246530302676
1107	22-23	0.15033097564420217
1107	24-25	0.14652801633119594
1107	26-27	0.21919366826708853
1107	28-29	0.22452350680969602
1107	30-31	0.15614760870520428
1107	32-33	0.23986746689205063
1107	34-35	0.17980506534320995
1107	36-37	0.11016675588579972
1107	38-39	0.13711752718396042
1107	40-41	0.19880577156130386
1107	42-43	0.2825711875429775
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2216	66-67	-0.8474740479002492
2216	68-69	-0.8069132410103137
2216	70-71	-0.825566944553664
2216	72-73	-0.753229973995694
2216	74-75	-0.683442704021374
2216	76-77	-0.7476421361581558
2216	78-79	-0.7232175291672505
2216	80-81	-0.7741969704854341
2216	82-83	-0.6740610127785303
2216	84-85	-0.7583449588505715
2216	86-87	-0.7672771308348487
2216	88-89	-0.66805797835395
2216	90-91	-0.6784509051933938
2216	92-93	-0.6657213557056636
2216	94-95	-0.6728374762600566
2216	96-97	-0.7002322319526257
2216	98-99	-0.7095631783479845
2216	100	-0.15882912426236118
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	7.0
12	13.0
13	64.0
14	305.0
15	911.0
16	2227.0
17	4512.0
18	8065.0
19	12855.0
20	18310.0
21	25544.0
22	34986.0
23	40666.0
24	41571.0
25	41179.0
26	43679.0
27	52522.0
28	66707.0
29	83878.0
30	104941.0
31	131534.0
32	165844.0
33	213643.0
34	304988.0
35	442280.0
36	717109.0
37	1349474.0
38	1194479.0
39	468738.0
40	1012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85435147317573	21.95829752959596	12.653597135988159	25.53375386124015
2	14.428709526539347	21.095754300279953	39.32427838683728	25.151257786343418
3	17.219803002273494	27.488171306615673	29.640218203589203	25.651807487521634
4	11.534952597377053	17.3311332638896	39.568432164914675	31.56548197381867
5	12.165294969730658	39.06820926560835	35.64878121270848	13.117714551952517
6	29.234442513375704	39.52642872166839	17.74725755934447	13.491871205611442
7	25.501449737295683	32.55668835350187	24.520068398598433	17.421793510604008
8	26.384231711020227	34.65225687377917	19.88959527239914	19.073916142801455
9	26.247423746115427	13.965970117967483	21.693008885069823	38.09359725084727
10-11	25.42094965509964	26.00123940155534	27.774538392015568	20.803272551329457
12-13	25.726828976766903	23.85524879220399	28.245525261486858	22.172396969542245
14-15	22.98953294089415	24.740351709124273	26.01226711973607	26.257848230245507
16-17	21.449830618710116	27.1277918839119	27.41386284817564	24.00851464920234
18-19	20.774095234950284	27.378336535780747	29.998947423300315	21.848620805968658
20-21	22.34236664319234	26.656169621058268	29.15965846644427	21.841805269305123
22-23	22.72612168891703	26.249361993552096	28.87222355028065	22.152292767250223
24-25	22.275416346317435	26.29685802911822	29.774235451119914	21.653490173444432
26-27	22.261516599653554	26.51123178496078	28.582114570523853	22.645137044861812
28-29	21.38384944555355	27.118037833154222	29.127112061570227	22.371000659722
30-31	22.356212549649644	26.734839136230796	29.068533916817596	21.84041439730196
32-33	22.906531247779093	26.87659142677265	28.209522394295522	22.007354931152733
34-35	22.629873346298055	26.6889583068619	29.090034105976194	21.591134240863855
36-37	22.39841429823598	26.215137568906492	28.714085890204743	22.672362242652785
38-39	22.497839213042823	26.262929725608767	29.47522489054286	21.76400617080554
40-41	22.0553175818425	25.544297568360914	29.91187793922661	22.48850691056998
42-43	22.244826135615583	25.80216523774758	28.940762133249486	23.012246493387345
44-45	22.37275943980342	26.815554579253142	28.741248274421377	22.070437706522057
46-47	22.503187340229186	26.60724682001793	27.89921436370567	22.99035147604721
48-49	23.429786268737292	26.431638012908724	27.827131300470707	22.311444417883276
50-51	22.673860795069096	26.302941972461092	27.69448697820764	23.32871025426217
52-53	21.991893818498205	27.030116775818712	27.500446873714562	23.47754253196852
54-55	22.143184798971436	26.92741658177861	27.278759823145688	23.650638796104268
56-57	23.373702720222596	26.3210053617667	27.0517605388615	23.253531379149194
58-59	22.109760988046844	26.77465637158073	28.07679085778853	23.038791782583896
60-61	22.296467867087912	27.4749355891662	27.109190092540548	23.119406451205336
62-63	22.71722010809678	26.637603723157966	26.688733972667837	23.95644219607742
64-65	21.82896324184204	26.923154922378544	27.22251555174925	24.025366284030163
66-67	22.56510790440518	26.41264054438963	27.176992764856166	23.845258786349017
68-69	22.053235760521634	26.286305348629696	27.614229176941173	24.0462297139075
70-71	22.06027722072885	26.071092325191824	27.586718071105963	24.28191238297336
72-73	22.692329504564405	26.001700279216212	28.073469147487828	23.232501068731555
74-75	22.75329846805067	26.533400777474085	27.450339585196996	23.262961169278242
76-77	22.502122877335783	26.50392724455412	26.985501620457796	24.008448257652297
78-79	22.207391238061945	26.560758185035706	27.12062459670317	24.111225980199187
80-81	21.77151687962263	27.102092158640527	27.742422708794116	23.383968252942726
82-83	22.6526477536789	25.934316024783723	28.82818226130303	22.58485396023434
84-85	23.020528911831995	26.543320548078945	27.180007910921017	23.25614262916804
86-87	21.42944312715406	27.22919991299423	27.719397047115923	23.621959912735793
88-89	22.552567783025403	26.047353538486057	27.45561592837386	23.944462750114678
90-91	22.13365508240782	26.024175329555906	27.650382875655684	24.19178671238059
92-93	22.581614574472134	26.3790899712924	27.270010789577398	23.76928466465807
94-95	21.320291081692822	26.427698704461054	27.944628577950926	24.307381635895194
96-97	21.706774031217268	26.302304863349967	27.46134093700624	24.529580168426524
98-99	21.002795383525328	27.114502326255856	27.318323401609895	24.56437888860892
100	21.876157762628377	26.36427650285334	26.35763185451596	25.40193388000232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	462.0
1	308.0
2	232.0
3	299.0
4	192.5
5	138.0
6	287.0
7	613.5
8	958.0
9	1013.0
10	763.0
11	589.0
12	600.5
13	743.5
14	988.0
15	1456.0
16	1987.5
17	2564.0
18	3522.5
19	4598.0
20	5944.5
21	7577.0
22	9448.5
23	11551.0
24	13481.5
25	15604.0
26	19022.0
27	22299.0
28	27005.0
29	37677.5
30	52894.0
31	66398.0
32	84766.0
33	103796.5
34	116553.5
35	132329.5
36	146834.0
37	170515.0
38	180368.0
39	167507.0
40	154747.5
41	142449.0
42	140151.0
43	148365.0
44	163876.5
45	197878.0
46	230614.0
47	254185.5
48	270195.5
49	262274.5
50	247061.5
51	251552.0
52	253925.5
53	241534.0
54	230973.5
55	196911.5
56	166510.0
57	135017.0
58	102670.5
59	102803.5
60	85503.5
61	54829.0
62	39259.0
63	32558.5
64	23574.0
65	10225.0
66	5018.5
67	3464.0
68	2566.0
69	1878.5
70	1495.5
71	1215.0
72	899.5
73	743.0
74	539.5
75	359.0
76	246.5
77	141.0
78	82.0
79	46.5
80	25.0
81	12.5
82	6.5
83	2.0
84	0.5
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007717096275621657
3	0.0
4	0.08878250064069847
5	0.004217482848304859
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.602276651081721E-4
14-15	0.010139905571456363
16-17	0.0
18-19	6.281357433645534E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	8.973367762350765E-6
60-61	0.0
62-63	0.0
64-65	3.589347104940306E-5
66-67	9.87070453858584E-5
68-69	0.0
70-71	3.948281815434336E-4
72-73	3.2304123944462747E-4
74-75	4.5764175587988896E-4
76-77	1.3460051643526146E-4
78-79	8.973367762350765E-6
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06555942487173469
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5572044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16954303426739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96328391352064	45.458877600623445
2	16.417843170621442	20.742153013928483
3	5.815266316666682	11.020431475392048
4	2.5933979697633633	6.552950586237939
5	1.2778589853113291	4.036088408217464
6	0.6803139265039574	2.57850719142619
7	0.4136836479687244	1.829254490205314
8	0.25450050013710246	1.2861344236522614
9	0.16210355071411073	0.9216006500558309
>10	0.4135393136839168	3.598782538341222
>50	0.003986738273785306	0.17326017432190002
>100	0.00310627637293019	0.41837871830397627
>500	6.198280748906885E-4	0.27267023113050265
>1k	3.718967903614478E-4	0.385538853306598
>5k	8.264373119142483E-5	0.41819349822780194
>10k+	4.1321865595712416E-5	0.30717814662909765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11768	0.2111971836546876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9852	0.17681123838935944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6169	0.11071341145188372	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.973367762350764E-5
2	0.0	0.0	0.0	0.0	8.973367762350764E-5
3	0.0	0.0	0.0	1.7946735524701526E-5	8.973367762350764E-5
4	0.0	0.0	0.0	3.589347104940305E-5	8.973367762350764E-5
5	0.0	0.0	0.0	3.589347104940305E-5	8.973367762350764E-5
6	1.7946735524701526E-5	0.0	0.0	3.589347104940305E-5	1.0768041314820917E-4
7	1.7946735524701526E-5	0.0	0.0	3.589347104940305E-5	1.0768041314820917E-4
8	1.7946735524701526E-5	0.0	0.0	3.589347104940305E-5	1.0768041314820917E-4
9	1.7946735524701526E-5	0.0	0.0	3.589347104940305E-5	1.0768041314820917E-4
10-11	1.7946735524701526E-5	0.0	0.0	8.973367762350764E-5	1.0768041314820917E-4
12-13	1.7946735524701526E-5	0.0	0.0	8.973367762350764E-5	1.8844072300936603E-4
14-15	3.589347104940305E-5	0.0	0.0	8.973367762350764E-5	2.602276651081721E-4
16-17	5.384020657410458E-5	0.0	0.0	8.973367762350764E-5	3.140678716822767E-4
18-19	5.384020657410458E-5	0.0	0.0	9.87070453858584E-5	3.230412394446275E-4
20-21	6.281357433645534E-5	0.0	0.0	1.0768041314820917E-4	3.5893471049403057E-4
22-23	1.0768041314820917E-4	0.0	0.0	1.435738841976122E-4	3.7688144601873206E-4
24-25	1.0768041314820917E-4	0.0	0.0	2.871477683952245E-4	3.7688144601873206E-4
26-27	1.0768041314820917E-4	0.0	0.0	6.101890078398519E-4	4.1277491706813516E-4
28-29	1.0768041314820917E-4	0.0	0.0	0.0031317053490604167	4.3072165259283666E-4
30-31	1.256271486729107E-4	0.0	0.0	0.011180816231889053	4.3072165259283666E-4
32-33	2.3330756182111985E-4	0.0	0.0	0.027628999340278	4.486683881175382E-4
34-35	2.602276651081721E-4	0.0	0.0	0.053723552793194025	4.486683881175382E-4
36-37	2.871477683952244E-4	0.0	0.0	0.09855449813389844	4.486683881175382E-4
38-39	2.961211361575752E-4	0.0	0.0	0.1673622821356041	4.666151236422397E-4
40-41	3.5893471049403057E-4	0.0	0.0	0.24160792700129433	4.666151236422397E-4
42-43	3.5893471049403057E-4	0.0	0.0	0.321569607131602	4.666151236422397E-4
44-45	3.8585481378108287E-4	0.0	0.0	0.4180063904735857	4.8456185916694126E-4
46-47	4.3072165259283666E-4	0.0	0.0	0.5366702057629121	5.025085946916428E-4
48-49	4.3072165259283666E-4	0.0	0.0	0.6745101079603822	5.025085946916428E-4
50-51	4.3072165259283666E-4	0.0	0.0	0.825540860768508	5.025085946916428E-4
52-53	4.666151236422397E-4	0.0	0.0	0.970388963188374	5.384020657410459E-4
54-55	4.8456185916694126E-4	0.0	0.0	1.131128899915363	5.473754335033966E-4
56-57	4.8456185916694126E-4	0.0	0.0	1.326855997547758	5.74295536790449E-4
58-59	4.8456185916694126E-4	0.0	0.0	1.5616710851529527	6.012156400775012E-4
60-61	4.8456185916694126E-4	0.0	0.0	1.806527371284218	6.371091111269042E-4
62-63	5.204553302163444E-4	0.0	0.0	2.0673113851936558	6.81975949938658E-4
64-65	5.204553302163444E-4	0.0	0.0	2.340936288371018	6.81975949938658E-4
66-67	5.204553302163444E-4	0.0	0.0	2.6518545079687095	6.909493177010088E-4
68-69	5.384020657410459E-4	0.0	0.0	2.99861235840923	6.999226854633595E-4
70-71	6.281357433645534E-4	0.0	0.0	3.380393263226206	7.268427887504119E-4
72-73	6.46082478889255E-4	0.0	0.0	3.7806772523691485	7.806829953245164E-4
74-75	6.46082478889255E-4	0.0	0.0	4.174993951950128	8.165764663739196E-4
76-77	6.46082478889255E-4	0.0	0.0	4.624496863269565	8.345232018986211E-4
78-79	6.46082478889255E-4	0.0	0.0	5.109830432064069	8.704166729480241E-4
80-81	7.537628920374641E-4	0.0	0.0	5.636522252875246	8.793900407103748E-4
82-83	7.537628920374641E-4	0.0	0.0	6.187172965611902	9.152835117597779E-4
84-85	7.717096275621657E-4	0.0	0.0	6.72146522891779	9.332302472844794E-4
86-87	7.98629730849218E-4	0.0	0.0	7.286859184887987	9.780970860962333E-4
88	8.255498341362703E-4	0.0	0.0	7.743154935603523	0.001022963924907987
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5880	0.0	44.38761	94
GGTATCA	10215	0.0	41.172306	1
GTATCAA	17545	0.0	38.58455	1
CTGTGCG	8150	0.0	33.97196	9
CAACGCA	19655	0.0	32.9277	5
ATCAACG	20245	0.0	31.949469	3
ACTGTGC	8970	0.0	31.698599	8
TAGTACT	8880	0.0	31.254877	4
GTACTAG	6485	0.0	31.120987	1
GTACTGT	9445	0.0	30.153656	6
TATCAAC	22550	0.0	29.958076	2
TACTGTG	10370	0.0	29.140806	7
AACGCAG	23420	0.0	28.89648	6
CTAGTAC	10285	0.0	27.945742	3
TAGGCAT	8090	0.0	27.71216	5
GTGGTAT	2425	0.0	27.159534	1
CTAACGC	3020	0.0	26.791697	3
CGCAGAG	24200	0.0	26.683851	8
ACGCAGA	24210	0.0	26.633524	7
TAACGCC	3100	0.0	26.252045	4
>>END_MODULE