FastQCFastQC Report
Mon 16 Jan 2017
ERR1146665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3860735
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC39350.042.7499294
GTACTAG43350.039.0461961
TAGGCAT54550.035.408695
GCATCGT47100.033.922888
CTGTGCG53650.033.7229589
TAGTACT56050.032.7015764
CTAGGCA61300.032.507614
ACTGTGC59950.031.511688
CAACGCA66150.031.4728935
GTATCAA77050.030.755531
TACTGTG65550.029.9670437
GTACTGT62900.029.810816
ATCAACG72100.028.8771133
TCTAACG21900.028.7575322
CTAACGC21900.028.7571583
CTAGTAC67500.028.6865773
TAACGCC22350.028.3880734
CATCGTC58700.027.5394349
CGACGAG28750.027.38004524-25
CGCCTAC23050.027.319597
Produced by FastQC (version 0.11.5)