##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146665_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3860735
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48137595561467	31.0	31.0	34.0	28.0	34.0
2	31.571970622174277	33.0	31.0	34.0	28.0	34.0
3	31.658917796740777	33.0	31.0	34.0	28.0	34.0
4	35.07599381982964	37.0	35.0	37.0	32.0	37.0
5	35.24070727465107	37.0	35.0	37.0	32.0	37.0
6	35.27426953675919	37.0	35.0	37.0	32.0	37.0
7	35.2345439404673	37.0	35.0	37.0	32.0	37.0
8	35.21771061727883	37.0	35.0	37.0	32.0	37.0
9	36.72302942315388	39.0	37.0	39.0	32.0	39.0
10-11	36.56568516098619	39.0	37.0	39.0	32.0	39.0
12-13	36.503941606973804	39.0	37.0	39.0	32.0	39.0
14-15	37.6964173920251	40.0	38.0	41.0	31.5	41.0
16-17	37.725022048910375	40.0	38.0	41.0	32.0	41.0
18-19	37.690516313603496	40.0	37.5	41.0	31.5	41.0
20-21	37.757567276697316	40.0	38.0	41.0	32.0	41.0
22-23	37.6934442534906	40.0	37.0	41.0	31.5	41.0
24-25	37.65550976692262	40.0	37.0	41.0	31.0	41.0
26-27	37.70548302331033	40.0	37.0	41.0	31.0	41.0
28-29	37.69127860368557	40.0	37.0	41.0	31.0	41.0
30-31	37.553458214562774	40.0	37.0	41.0	31.0	41.0
32-33	37.551521277684174	40.0	37.0	41.0	31.0	41.0
34-35	37.48885069811837	40.0	37.0	41.0	31.0	41.0
36-37	37.370630592361294	40.0	36.5	41.0	30.0	41.0
38-39	37.26794237366719	40.0	36.0	41.0	30.0	41.0
40-41	37.18305970236237	40.0	36.0	41.0	30.0	41.0
42-43	37.01135651631101	40.0	35.5	41.0	30.0	41.0
44-45	36.85485445128971	39.0	35.0	41.0	30.0	41.0
46-47	36.84953085358099	39.0	35.0	41.0	30.0	41.0
48-49	36.71158289807511	39.0	35.0	41.0	30.0	41.0
50-51	36.52760821449802	39.0	35.0	41.0	29.5	41.0
52-53	36.31479990209118	39.0	35.0	41.0	29.0	41.0
54-55	36.09540320690232	38.0	35.0	40.0	29.0	41.0
56-57	35.85355936110611	38.0	34.0	40.0	28.5	41.0
58-59	35.55694938917071	37.0	34.0	40.0	28.0	41.0
60-61	35.30938033819985	37.0	34.0	40.0	28.0	41.0
62-63	35.00090565656539	36.0	34.0	40.0	27.5	41.0
64-65	34.84402943999005	36.0	34.0	39.0	28.0	41.0
66-67	34.590735572371585	35.0	33.0	39.0	27.5	41.0
68-69	34.466771741650234	35.0	33.0	39.0	27.5	41.0
70-71	34.37729603300926	35.0	33.5	38.5	28.0	41.0
72-73	34.15489019577878	35.0	33.0	37.5	27.5	40.5
74-75	33.901300659071396	35.0	33.0	37.0	27.0	40.0
76-77	33.63278585554305	35.0	33.0	36.5	27.0	39.0
78-79	33.3757981317029	35.0	33.0	36.0	26.0	39.0
80-81	33.184778546054055	35.0	33.0	36.0	26.0	38.5
82-83	32.92441076634371	35.0	33.0	35.5	26.0	37.0
84-85	32.742696921700144	35.0	33.0	35.0	26.0	37.0
86-87	32.57025903098762	35.0	33.0	35.0	26.0	36.5
88-89	32.43548767268409	35.0	33.0	35.0	25.0	36.0
90-91	32.28138748191731	35.0	32.5	35.0	25.0	36.0
92-93	32.12270824078835	35.0	32.0	35.0	25.0	36.0
94-95	32.04885973784785	35.0	32.0	35.0	25.0	35.5
96-97	31.9345750226317	35.0	32.0	35.0	25.0	35.0
98-99	31.841521497849502	35.0	32.0	35.0	24.5	35.0
100	30.889595633991974	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3439335349661228
1101	2	0.38089140624508033
1101	3	0.424392175528034
1101	4	0.46367805095867
1101	5	0.38324780029780214
1101	6	0.3614655866735177
1101	7	0.3650338742152215
1101	8	0.42268332836196265
1101	9	0.5668490048620001
1101	10-11	0.5803965783421035
1101	12-13	0.4762055031413084
1101	14-15	0.6724540790332298
1101	16-17	0.6290372226250511
1101	18-19	0.6157808914599343
1101	20-21	0.5506778512572978
1101	22-23	0.6308967390907299
1101	24-25	0.6601529710561493
1101	26-27	0.5780759090928029
1101	28-29	0.5973323242733812
1101	30-31	0.6828936670903687
1101	32-33	0.6326233758768964
1101	34-35	0.6696498896114562
1101	36-37	0.6336812752730339
1101	38-39	0.5708722253991496
1101	40-41	0.5641505650456224
1101	42-43	0.5626230571625044
1101	44-45	0.6053762874692055
1101	46-47	0.5659845066219162
1101	48-49	0.5325876867335921
1101	50-51	0.4964655125355719
1101	52-53	0.508875768123076
1101	54-55	0.47548323977117235
1101	56-57	0.4475083270779834
1101	58-59	0.4645890539749402
1101	60-61	0.46244418226174844
1101	62-63	0.432501269670972
1101	64-65	0.4529904527918305
1101	66-67	0.567934720391726
1101	68-69	0.5894706556522422
1101	70-71	0.5261390658099074
1101	72-73	0.4857235118073646
1101	74-75	0.43127369226588996
1101	76-77	0.44081613576555867
1101	78-79	0.42394061641650893
1101	80-81	0.3899593995932662
1101	82-83	0.41286415965215895
1101	84-85	0.4029304855521829
1101	86-87	0.3833832414246814
1101	88-89	0.4352194179941833
1101	90-91	0.3861373172406388
1101	92-93	0.4327988421033737
1101	94-95	0.42725030647768847
1101	96-97	0.3964776648935384
1101	98-99	0.3667776608324651
1101	100	0.4170902636343996
1102	1	0.3429673862978113
1102	2	0.3861109378813836
1102	3	0.40913546070037654
1102	4	0.4790398777261373
1102	5	0.344135206905527
1102	6	0.4255108580712843
1102	7	0.3627962487228942
1102	8	0.4051576432838786
1102	9	0.533404273852895
1102	10-11	0.5975583998728595
1102	12-13	0.5586430351784131
1102	14-15	0.6807295357603422
1102	16-17	0.621712808658728
1102	18-19	0.7273366337692693
1102	20-21	0.631153283261078
1102	22-23	0.7545600865699029
1102	24-25	0.6869774436837375
1102	26-27	0.6442900111624184
1102	28-29	0.623250662744411
1102	30-31	0.6278446245027851
1102	32-33	0.5803658105866489
1102	34-35	0.6444839826880084
1102	36-37	0.672543488248202
1102	38-39	0.6392855902501182
1102	40-41	0.5804714595872511
1102	42-43	0.6254792104028795
1102	44-45	0.5840805769930597
1102	46-47	0.5191661065691449
1102	48-49	0.5514627070006881
1102	50-51	0.6111261797232288
1102	52-53	0.5060661322660778
1102	54-55	0.5362002076832013
1102	56-57	0.5173798211227307
1102	58-59	0.5081446724158241
1102	60-61	0.5539127811082523
1102	62-63	0.5387456175439027
1102	64-65	0.5277672755965241
1102	66-67	0.5699809414475467
1102	68-69	0.552647803133226
1102	70-71	0.5016094637700022
1102	72-73	0.4748107839221589
1102	74-75	0.36981993371468036
1102	76-77	0.437267128676865
1102	78-79	0.4079745533483461
1102	80-81	0.4535396922752568
1102	82-83	0.4197911938388472
1102	84-85	0.48854777965111396
1102	86-87	0.36835044020533303
1102	88-89	0.4460238239342331
1102	90-91	0.4092966561154867
1102	92-93	0.3684236010382662
1102	94-95	0.38388162364233125
1102	96-97	0.3949942018340238
1102	98-99	0.3141601972956103
1102	100	0.3687342395495392
1103	1	0.26844283414089887
1103	2	0.2942729187466213
1103	3	0.302298075269384
1103	4	0.3799198295028319
1103	5	0.26434534925703446
1103	6	0.30713746744701353
1103	7	0.3020462525869405
1103	8	0.3343762092586289
1103	9	0.417681619716042
1103	10-11	0.5565756644364512
1103	12-13	0.5138502253977393
1103	14-15	0.6449450877781473
1103	16-17	0.5713070218614078
1103	18-19	0.5881327820868592
1103	20-21	0.4781324946862995
1103	22-23	0.5944029898605265
1103	24-25	0.5406092772514981
1103	26-27	0.4713399810228509
1103	28-29	0.46731421859534805
1103	30-31	0.4569174076988105
1103	32-33	0.44995229569460804
1103	34-35	0.5227527039974618
1103	36-37	0.5689922741645219
1103	38-39	0.59485525902398
1103	40-41	0.4789349288835396
1103	42-43	0.47986314214833925
1103	44-45	0.43901728389733563
1103	46-47	0.3994614434367918
1103	48-49	0.45886857107895906
1103	50-51	0.41802872844886707
1103	52-53	0.407890117180834
1103	54-55	0.4096211570023556
1103	56-57	0.3988293025675773
1103	58-59	0.4574864551327167
1103	60-61	0.4008596890975582
1103	62-63	0.4017302695478051
1103	64-65	0.3428416044114968
1103	66-67	0.403569281298239
1103	68-69	0.37225221742093595
1103	70-71	0.3512644507095217
1103	72-73	0.3313384582293324
1103	74-75	0.30823307765817276
1103	76-77	0.24935056118741272
1103	78-79	0.29867703715797944
1103	80-81	0.3280093749596915
1103	82-83	0.3722626385288734
1103	84-85	0.35309131200864385
1103	86-87	0.35175955008338633
1103	88-89	0.3723092997530202
1103	90-91	0.3442398237069497
1103	92-93	0.24584912538949055
1103	94-95	0.2987222857854803
1103	96-97	0.39595820436883855
1103	98-99	0.4418103619040288
1103	100	0.4247045018410738
1104	1	0.2603044321377119
1104	2	0.33383494924969526
1104	3	0.3269973696012727
1104	4	0.41946971947930933
1104	5	0.29406717944142
1104	6	0.2664621506551441
1104	7	0.28528048887194046
1104	8	0.33059352468468006
1104	9	0.38508734093920083
1104	10-11	0.5297437348515075
1104	12-13	0.3936172790436885
1104	14-15	0.48608462795569807
1104	16-17	0.47259573875950167
1104	18-19	0.4377685662890016
1104	20-21	0.44290463217776477
1104	22-23	0.4641756585000252
1104	24-25	0.5205132153348089
1104	26-27	0.4288082259579724
1104	28-29	0.37797678783428523
1104	30-31	0.3559135379059626
1104	32-33	0.4297841494012644
1104	34-35	0.48656118719041785
1104	36-37	0.47096207460461414
1104	38-39	0.4844984792986651
1104	40-41	0.4287757362285518
1104	42-43	0.48139542836126026
1104	44-45	0.40889360228265303
1104	46-47	0.42954809644536596
1104	48-49	0.37260314881626755
1104	50-51	0.32804860856116136
1104	52-53	0.2836272747800521
1104	54-55	0.3878211721780147
1104	56-57	0.38050090132317393
1104	58-59	0.30815084545064764
1104	60-61	0.35439728679085647
1104	62-63	0.3871029078359882
1104	64-65	0.37003835682062913
1104	66-67	0.38042093923890263
1104	68-69	0.3698653898922899
1104	70-71	0.38086927656884484
1104	72-73	0.3385268640264343
1104	74-75	0.30650441842757914
1104	76-77	0.2987430795878936
1104	78-79	0.3141250984676418
1104	80-81	0.23896286156717395
1104	82-83	0.25584637022323165
1104	84-85	0.3079519235876731
1104	86-87	0.2791321883715696
1104	88-89	0.2651066288371666
1104	90-91	0.32226182841140627
1104	92-93	0.3768124761748268
1104	94-95	0.38440056183070936
1104	96-97	0.28809569583253847
1104	98-99	0.32863804230467153
1104	100	0.3935473578999584
1105	1	0.14186303957698598
1105	2	0.22643783371932358
1105	3	0.20534034683533164
1105	4	0.27818078845285754
1105	5	0.17391679379003477
1105	6	0.1896289200246457
1105	7	0.20532619836015442
1105	8	0.22074511526304974
1105	9	0.3863176820172569
1105	10-11	0.3892849775886873
1105	12-13	0.2603023933518003
1105	14-15	0.4602699792128391
1105	16-17	0.38503463655329284
1105	18-19	0.3628078310622911
1105	20-21	0.3135140411924695
1105	22-23	0.316196093822505
1105	24-25	0.31447931173913446
1105	26-27	0.355013145468547
1105	28-29	0.36441120856190423
1105	30-31	0.4239804463353991
1105	32-33	0.33184387192122955
1105	34-35	0.3437723463092439
1105	36-37	0.2224275558384008
1105	38-39	0.2884817645993607
1105	40-41	0.26562208950058874
1105	42-43	0.2699577112763194
1105	44-45	0.2748401083083323
1105	46-47	0.22583203285511644
1105	48-49	0.07732133493173876
1105	50-51	0.2231596995403251
1105	52-53	0.29933494813182193
1105	54-55	0.2537106758886196
1105	56-57	0.21631595071173138
1105	58-59	0.2886479660339418
1105	60-61	0.2963965417094556
1105	62-63	0.3330681664722235
1105	64-65	0.3090284037649482
1105	66-67	0.20011442435117743
1105	68-69	0.15684068231132642
1105	70-71	0.25055516735223193
1105	72-73	0.28322733801421407
1105	74-75	0.23345368681565049
1105	76-77	0.17476032349830461
1105	78-79	0.27118420656454134
1105	80-81	0.1946667108454534
1105	82-83	0.22338628006599492
1105	84-85	0.14549697006705742
1105	86-87	0.13675583316508266
1105	88-89	0.13095760777307675
1105	90-91	0.21766153378732156
1105	92-93	0.2348928122149161
1105	94-95	0.18327790175855085
1105	96-97	0.21680400812449108
1105	98-99	0.16457919044162495
1105	100	0.3690007826881434
1106	1	-0.025537369686361444
1106	2	0.07518119647959409
1106	3	0.08969392540502241
1106	4	0.20039405781869135
1106	5	0.06674757815944332
1106	6	0.11786699307205595
1106	7	0.052739411909470846
1106	8	0.08379830765293406
1106	9	0.2053522700569772
1106	10-11	0.21942939271565365
1106	12-13	0.1275792377886802
1106	14-15	0.2015106717652415
1106	16-17	0.1384302713665022
1106	18-19	0.128146712726938
1106	20-21	0.11315793643422722
1106	22-23	0.21292296933116006
1106	24-25	0.23813944808512844
1106	26-27	0.22603856596309413
1106	28-29	0.19769049251571857
1106	30-31	0.27728327029871025
1106	32-33	0.22947528125073546
1106	34-35	0.24634402300912228
1106	36-37	0.23710650541314493
1106	38-39	0.20397821525244098
1106	40-41	0.26893654190501337
1106	42-43	0.15009585715677787
1106	44-45	0.2427235673492092
1106	46-47	0.1985223716573401
1106	48-49	0.12762199874960345
1106	50-51	0.15466865806246943
1106	52-53	0.10540019856509986
1106	54-55	0.14220551069397658
1106	56-57	0.08933447156823604
1106	58-59	0.1016242951503088
1106	60-61	0.08316425688111906
1106	62-63	0.17387749441765976
1106	64-65	0.15194168506256744
1106	66-67	0.1118761952948617
1106	68-69	0.21821995699128394
1106	70-71	0.20586464133933902
1106	72-73	0.05600859533615932
1106	74-75	0.10412632496369412
1106	76-77	0.07925407317509325
1106	78-79	0.14055620718510653
1106	80-81	0.15088099311351755
1106	82-83	0.12084391281584317
1106	84-85	0.12894936611818508
1106	86-87	0.12897591491952198
1106	88-89	0.1300566419335354
1106	90-91	0.12272299696012112
1106	92-93	0.1607260225227165
1106	94-95	0.1656820335675846
1106	96-97	0.1694775239670534
1106	98-99	0.18865631835245367
1106	100	0.12230549898162835
1107	1	0.053993894086506344
1107	2	0.10760893454335019
1107	3	0.09184570762287336
1107	4	0.17771096992233737
1107	5	0.10174023022271683
1107	6	0.18225240355221928
1107	7	0.1372713187171115
1107	8	0.14600737054173862
1107	9	0.13837607968400079
1107	10-11	0.21952331914380352
1107	12-13	0.1292911553183842
1107	14-15	0.27018490745699353
1107	16-17	0.18610848231369914
1107	18-19	0.12778480697416228
1107	20-21	0.20960404939737032
1107	22-23	0.258625112732247
1107	24-25	0.30642150481380526
1107	26-27	0.2474118859444232
1107	28-29	0.25346927988218226
1107	30-31	0.419783172759729
1107	32-33	0.32864034755557014
1107	34-35	0.2953926205430477
1107	36-37	0.2834790667579412
1107	38-39	0.3324668234217327
1107	40-41	0.2734939244500154
1107	42-43	0.22678859201980828
1107	44-45	0.3114829148350893
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2216	96-97	-1.0540944142816926
2216	98-99	-1.05535040294102
2216	100	-0.7258626677409481
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	12.0
3	24.0
4	27.0
5	28.0
6	59.0
7	155.0
8	448.0
9	719.0
10	986.0
11	1733.0
12	2880.0
13	4291.0
14	6070.0
15	8037.0
16	10267.0
17	12049.0
18	13764.0
19	15938.0
20	18443.0
21	21179.0
22	24758.0
23	28526.0
24	32757.0
25	38107.0
26	43508.0
27	47560.0
28	55079.0
29	66940.0
30	79176.0
31	92110.0
32	114025.0
33	148539.0
34	214558.0
35	307605.0
36	500856.0
37	876414.0
38	771789.0
39	300589.0
40	729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65988384373616	20.35601073288232	11.601864284968237	24.382241138413285
2	15.92378252457565	19.721341706500343	39.881779917092075	24.473095851831935
3	18.83843081851181	26.198382409434384	28.742086703745407	26.221100068308402
4	11.95172805571681	16.11833967597482	40.18032944433694	31.74960282397143
5	12.903714617418185	38.792090112744546	35.27376413839764	13.030431131439633
6	30.19682777491987	38.50437518811677	17.89413345061977	13.40466358634359
7	27.26652256517916	31.785153229785752	23.421240421114796	17.527083783920293
8	26.7258635534295	34.884486551059965	19.73100047763656	18.658649417873967
9	26.379714192393163	14.707194973772953	21.10102056383944	37.81207026999445
10-11	25.93334156152305	25.733352518052694	27.551061507703956	20.782244412720296
12-13	25.717718453548926	23.61579377544131	27.693837696483577	22.972650074526186
14-15	23.10073188344984	24.92730638087571	24.933134303306844	27.038827432367608
16-17	22.19274721395122	27.622047191066823	26.2070352612648	23.97817033371716
18-19	21.545238967898015	28.443687810260666	27.73344747601773	22.27762574582358
20-21	23.27441222431482	27.441287186594966	26.836228361755214	22.448072227334997
22-23	23.648891568898968	27.19724131643062	26.459314701392138	22.694552413278277
24-25	23.101397669915535	27.14294428526821	27.465154737817254	22.290503306998996
26-27	23.081388338488225	27.29768118528565	26.321556277354276	23.29937419887185
28-29	22.17159905780301	27.90426445548	26.829184897546376	23.094951589170616
30-31	22.964660786079726	27.615225389256675	26.825793763966782	22.594320060696816
32-33	23.46224349030496	27.65375881356944	25.88373360798155	23.00026408814405
34-35	23.364398226222548	27.467673528900942	26.99328817511487	22.17464006976164
36-37	22.951583633573755	27.029979055162595	26.6215087458393	23.39692856542435
38-39	23.18834253969763	27.215579165558744	27.194545421812695	22.401532872930932
40-41	22.782835227723833	26.3233308116239	27.783137651530843	23.110696309121426
42-43	22.767706391882054	26.549306456232074	27.11334062236368	23.569646529522192
44-45	22.871240470019615	27.520049617557234	27.022565729273424	22.58614418314972
46-47	22.851420797184247	27.339634976542087	26.54080046922298	23.268143757050684
48-49	23.53925283571433	26.983510342565587	26.688902280229872	22.788334541490215
50-51	23.077930447116426	26.665962869291228	26.85929125877183	23.396815424820517
52-53	22.529884715301435	27.36088538399777	26.624257173199606	23.484972727501194
54-55	22.530521211317314	27.2074113346975	26.650219256345792	23.611848197639393
56-57	23.645089915435424	26.67708860356149	26.3546510257305	23.323170455272585
58-59	22.613795850428914	27.089672056294788	27.24411356107292	23.05241853220338
60-61	22.52481124490605	27.74666526946426	26.543210276328725	23.185313209300965
62-63	22.97480487116186	27.079996995028843	26.08813632207074	23.857061811738557
64-65	22.164018213740093	27.30645310661359	26.61998602430269	23.909542655343632
66-67	22.802226376612346	26.88600654866938	26.550840266635685	23.76092680808259
68-69	22.31822719612403	26.90138287571629	26.879365036604007	23.901024891555668
70-71	22.257234865788774	26.641447028852166	27.013252549670668	24.088065555688388
72-73	22.9338158438386	26.603209800109575	27.495068471504634	22.96790588454719
74-75	23.046367120116614	26.889919505359362	26.992334966298877	23.07137840822515
76-77	22.882255356209356	26.783818608258557	26.67999210421197	23.653933931320115
78-79	22.69729611615337	26.741522765686238	26.692250790210316	23.86893032795007
80-81	22.18153162826591	27.1324848839351	27.42605037902524	23.259933108773755
82-83	23.055928136949696	26.192670602991253	28.39331850606172	22.358082753997326
84-85	23.270245965223	26.69195238741009	27.09659904335992	22.94120260400699
86-87	21.986444474061837	27.36292058908214	27.499501663646857	23.151133273209165
88-89	23.18016902972915	26.15244952188719	27.230839092256577	23.43654235612708
90-91	22.786804025965026	26.181285000683257	27.42189670592853	23.610014267423193
92-93	23.15833347472791	26.421970722644584	27.337495212552316	23.08220059007519
94-95	21.9943604735275	26.482590477927264	27.953941924352737	23.569107124192495
96-97	22.35938467165698	26.364919927503898	27.524893821032343	23.750801579806783
98-99	21.748279726725002	27.05200718948399	27.347135066789747	23.85257801700126
100	22.67099246473884	26.29329866600739	26.510220490010116	24.525488379243647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	749.0
1	526.5
2	373.5
3	455.5
4	417.5
5	348.5
6	317.5
7	334.0
8	364.5
9	361.5
10	335.0
11	299.0
12	302.5
13	340.0
14	382.5
15	543.5
16	667.5
17	744.0
18	963.5
19	1199.0
20	1553.5
21	2175.5
22	3171.0
23	4437.0
24	5937.5
25	7552.0
26	9808.5
27	12507.0
28	16092.5
29	23497.5
30	34498.5
31	44734.5
32	57970.5
33	69893.5
34	77310.5
35	88236.0
36	98007.0
37	113913.5
38	120964.0
39	111783.5
40	104220.5
41	97471.0
42	96611.5
43	103437.0
44	114037.5
45	135951.5
46	156180.0
47	169747.0
48	180359.5
49	178417.5
50	169885.5
51	174135.0
52	178234.5
53	171814.5
54	163030.0
55	139757.0
56	121773.5
57	101777.0
58	80369.5
59	77807.5
60	65926.0
61	46094.0
62	34834.5
63	28875.0
64	20930.0
65	10792.5
66	6390.0
67	4533.5
68	3303.0
69	2479.0
70	1971.0
71	1537.0
72	1171.5
73	959.5
74	725.5
75	517.0
76	331.5
77	180.5
78	113.5
79	77.0
80	49.0
81	32.5
82	26.0
83	21.5
84	17.5
85	16.5
86	17.5
87	13.5
88	12.5
89	12.5
90	6.5
91	5.0
92	8.5
93	6.5
94	2.5
95	2.5
96	4.0
97	3.5
98	4.5
99	6.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05988497008989221
2	0.007977755531006402
3	0.007822344708973809
4	0.00922104210726714
5	0.003859368747142707
6	0.002616082170881969
7	7.770541101629612E-4
8	0.0015282064166538237
9	6.734468954745664E-4
10-11	6.086923862943196E-4
12-13	2.9787074222913513E-4
14-15	2.849198403930858E-4
16-17	6.60495993638517E-4
18-19	0.0018131262570469093
20-21	9.324649321955534E-4
22-23	0.0013727955946212314
24-25	0.001541157318489873
26-27	0.004234944900388139
28-29	0.0047529809738301124
30-31	0.006112825666615295
32-33	0.0072913577336957865
34-35	0.004157239489371843
36-37	0.009233993009103188
38-39	0.007032339696974799
40-41	0.006695616249237516
42-43	0.015450425890406879
44-45	0.02100636277807205
46-47	0.019076678404500697
48-49	0.022301452961676987
50-51	0.018714053153091315
52-53	0.025539178420689326
54-55	0.01956881267427057
56-57	0.01574829663263601
58-59	0.018066508061288847
60-61	0.01659010525197922
62-63	0.012044338707525899
64-65	0.011979584198345652
66-67	0.014543862761883426
68-69	0.005931513040910604
70-71	0.009933341708249853
72-73	0.009583667358676521
74-75	0.01238106215526318
76-77	0.011629909848772319
78-79	0.013689103240704166
80-81	0.011344990008379234
82-83	0.007278406831859736
84-85	0.011746467965296764
86-87	0.010114654333954545
88-89	0.00903972948156245
90-91	0.014051728492113549
92-93	0.009829734493561459
94-95	0.007887099218154055
96-97	0.010088752530282446
98-99	0.009700225475200965
100	0.2600282070641989
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3860735.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.41907605968467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32441546185838	52.983704021577196
2	14.647768888942272	20.336691652123328
3	4.756545543208657	9.905849905360677
4	1.9905495216157272	5.5272843456644445
5	0.9205168541152685	3.1950714755024743
6	0.5063713344736874	2.1091098109363764
7	0.28053001044270093	1.3631893892362195
8	0.1722627028328065	0.956665413615796
9	0.11032162397148343	0.6892582684953905
>10	0.2883816033551989	2.7539124628052694
>50	0.0018321965272809822	0.08470646432813403
>100	4.542129274255167E-4	0.0569667224078224
>500	0.0	0.0
>1k	5.004572911723281E-5	0.03759006794687788
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	3.885270550814806E-5
14-15	0.0	0.0	0.0	1.2950901836049353E-5	1.0360721468839483E-4
16-17	0.0	0.0	0.0	2.5901803672098707E-5	1.2950901836049353E-4
18-19	0.0	0.0	0.0	2.5901803672098707E-5	1.8131262570469096E-4
20-21	0.0	0.0	0.0	5.1803607344197414E-5	1.942635275407403E-4
22-23	0.0	0.0	0.0	1.1655811652444418E-4	2.0721442937678966E-4
24-25	0.0	0.0	0.0	1.8131262570469096E-4	2.0721442937678966E-4
26-27	0.0	0.0	0.0	4.5328156426172733E-4	2.3311623304888836E-4
28-29	0.0	0.0	0.0	0.0026808366800622164	2.3311623304888836E-4
30-31	0.0	0.0	0.0	0.010295966959659236	2.3311623304888836E-4
32-33	0.0	0.0	0.0	0.026173772610655743	2.3311623304888836E-4
34-35	0.0	0.0	0.0	0.051700000129509024	2.3311623304888836E-4
36-37	0.0	0.0	0.0	0.09744258541443535	2.3311623304888836E-4
38-39	0.0	0.0	0.0	0.16660040121893888	2.5901803672098706E-4
40-41	0.0	0.0	0.0	0.24161202465333673	2.5901803672098706E-4
42-43	0.0	0.0	0.0	0.3192785829641247	2.719689385570364E-4
44-45	0.0	0.0	0.0	0.41151490584046824	2.9787074222913513E-4
46-47	0.0	0.0	0.0	0.5284356476163218	3.2377254590123383E-4
48-49	0.0	0.0	0.0	0.6680722712126059	3.4967434957333253E-4
50-51	0.0	0.0	0.0	0.8187948667805482	4.40330662425678E-4
52-53	0.0	0.0	0.0	0.9663315404968225	4.40330662425678E-4
54-55	0.0	0.0	0.0	1.127104035889539	4.40330662425678E-4
56-57	0.0	0.0	0.0	1.3203962457925758	4.40330662425678E-4
58-59	0.0	0.0	0.0	1.5595994027044073	4.40330662425678E-4
60-61	0.0	0.0	0.0	1.8101864023301264	4.40330662425678E-4
62-63	0.0	0.0	0.0	2.065409824813151	4.40330662425678E-4
64-65	0.0	0.0	0.0	2.333117916666127	4.40330662425678E-4
66-67	0.0	0.0	0.0	2.6399377320639723	4.40330662425678E-4
68-69	0.0	0.0	0.0	2.988122727926159	4.40330662425678E-4
70-71	0.0	0.0	0.0	3.3768051938296724	4.40330662425678E-4
72-73	0.0	0.0	0.0	3.7747346036441245	4.921342697698755E-4
74-75	0.0	0.0	0.0	4.164841668749603	5.568887789501222E-4
76-77	0.0	0.0	0.0	4.6134350065466805	5.698396807861715E-4
78-79	0.0	0.0	0.0	5.097125288319452	5.827905826222209E-4
80-81	0.0	0.0	0.0	5.616029072184442	5.957414844582703E-4
82-83	0.0	0.0	0.0	6.156068727845864	6.216432881303689E-4
84-85	0.0	0.0	0.0	6.679401720138782	6.216432881303689E-4
86-87	0.0	0.0	0.0	7.22945242291947	6.60495993638517E-4
88	0.0	0.0	0.0	7.6811021735498555	6.993486991466651E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3935	0.0	42.74992	94
GTACTAG	4335	0.0	39.046196	1
TAGGCAT	5455	0.0	35.40869	5
GCATCGT	4710	0.0	33.92288	8
CTGTGCG	5365	0.0	33.722958	9
TAGTACT	5605	0.0	32.701576	4
CTAGGCA	6130	0.0	32.50761	4
ACTGTGC	5995	0.0	31.51168	8
CAACGCA	6615	0.0	31.472893	5
GTATCAA	7705	0.0	30.75553	1
TACTGTG	6555	0.0	29.967043	7
GTACTGT	6290	0.0	29.81081	6
ATCAACG	7210	0.0	28.877113	3
TCTAACG	2190	0.0	28.757532	2
CTAACGC	2190	0.0	28.757158	3
CTAGTAC	6750	0.0	28.686577	3
TAACGCC	2235	0.0	28.388073	4
CATCGTC	5870	0.0	27.539434	9
CGACGAG	2875	0.0	27.380045	24-25
CGCCTAC	2305	0.0	27.31959	7
>>END_MODULE