FastQCFastQC Report
Mon 16 Jan 2017
ERR1146665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3860735
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96800.25072945954591547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82000.2123947901112094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54460.14106122279824956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC37450.042.8135594
GGTATCA78550.041.5650561
GTATCAA134550.037.447331
CTGTGCG53150.031.9270529
CTAACGC21550.031.6516513
CAACGCA152700.031.6403665
ATCAACG156450.030.9396553
TCTAACG22200.030.939622
TAGTACT56150.030.8300134
TAACGCC22500.030.315254
CGCCTAC22850.030.2348127
TAGGCAT54500.030.1827585
ACTGTGC60600.029.7806878
GTGGTAT23900.029.1325761
TATCAAC172900.028.9780852
AACGCAG172950.028.7498516
GTACTGT61750.028.4646056
CTAGGCA60050.028.2793794
TACTGTG68350.027.7103927
CTAGTAC64550.027.5468043
Produced by FastQC (version 0.11.5)