FastQCFastQC Report
Mon 16 Jan 2017
ERR1146664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4611556
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC56000.043.0730694
GTACTAG69250.035.5116541
TATTCTC14100.035.328575
GTACCGT1353.4742698E-1034.8086136
TAGGCAT85950.031.383795
TACCCCG3150.031.3291135
CAGTACG7650.030.7131529
GCATCGT74600.030.2985658
CTGTGCG86200.030.0372479
TAGTACT86500.029.9909734
ACTGTGC89850.028.9739348
CTAGGCA97800.028.25574
AACGCGT1005.3338255E-528.1946728
CAACGCA93900.028.0760885
CTAACGC34800.027.9549483
TAACGCC35500.027.9332664
TCTAACG35000.027.9297852
CGCCTAC35500.027.7975657
GTACTGT93400.027.7220446
TATTCGC7150.027.6031749
Produced by FastQC (version 0.11.5)