##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146664_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4611556 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47202050674436 31.0 31.0 34.0 28.0 34.0 2 31.611534154632405 33.0 31.0 34.0 28.0 34.0 3 31.72808939108622 33.0 31.0 34.0 28.0 34.0 4 35.17225444080046 37.0 35.0 37.0 32.0 37.0 5 35.322004113145326 37.0 35.0 37.0 32.0 37.0 6 35.41718109896096 37.0 35.0 37.0 32.0 37.0 7 35.36971317273389 37.0 35.0 37.0 32.0 37.0 8 35.34501847098897 37.0 35.0 37.0 32.0 37.0 9 36.896309185012605 39.0 37.0 39.0 33.0 39.0 10-11 36.73753555199156 39.0 37.0 39.0 32.0 39.0 12-13 36.667788269295656 39.0 37.0 39.0 32.0 39.0 14-15 37.86616632216979 40.0 38.0 41.0 32.0 41.0 16-17 37.881465496678345 40.0 38.0 41.0 32.0 41.0 18-19 37.8323481271831 40.0 38.0 41.0 32.0 41.0 20-21 37.87704865776324 40.0 38.0 41.0 32.0 41.0 22-23 37.79322109500568 40.0 37.5 41.0 32.0 41.0 24-25 37.72570895810438 40.0 37.0 41.0 32.0 41.0 26-27 37.73967344210934 40.0 37.0 41.0 32.0 41.0 28-29 37.7095899952207 40.0 37.0 41.0 32.0 41.0 30-31 37.567055783340805 40.0 37.0 41.0 31.0 41.0 32-33 37.55364035479565 40.0 37.0 41.0 31.0 41.0 34-35 37.48626602387567 40.0 37.0 41.0 31.0 41.0 36-37 37.36358975148518 40.0 36.0 41.0 31.0 41.0 38-39 37.242653238950155 40.0 36.0 41.0 30.0 41.0 40-41 37.14272720530771 40.0 36.0 41.0 30.0 41.0 42-43 36.969588789553896 39.5 35.5 41.0 30.0 41.0 44-45 36.78710970874039 39.0 35.0 41.0 30.0 41.0 46-47 36.75549348202646 39.0 35.0 41.0 30.0 41.0 48-49 36.571848417323785 39.0 35.0 41.0 30.0 41.0 50-51 36.34942566023268 39.0 35.0 41.0 29.0 41.0 52-53 36.08958787446146 38.0 35.0 40.0 29.0 41.0 54-55 35.81961663265067 38.0 34.5 40.0 28.5 41.0 56-57 35.52681415990611 37.0 34.0 40.0 28.0 41.0 58-59 35.160218915264174 36.5 34.0 40.0 28.0 41.0 60-61 34.87939168471553 36.0 34.0 39.0 28.0 41.0 62-63 34.530244347027335 35.0 33.0 39.0 27.0 41.0 64-65 34.3463914349083 35.0 33.0 39.0 27.0 41.0 66-67 34.078234548165526 35.0 33.0 38.0 26.5 40.5 68-69 33.93310511679789 35.0 33.0 37.0 26.5 40.0 70-71 33.87125159056943 35.0 33.0 37.0 27.0 40.0 72-73 33.6574704936902 35.0 33.0 36.0 27.0 39.5 74-75 33.4284992961161 35.0 33.0 36.0 26.5 39.0 76-77 33.2056938048676 35.0 33.0 35.5 26.0 39.0 78-79 32.98342110558779 35.0 33.0 35.0 26.0 37.5 80-81 32.84535805268331 35.0 33.0 35.0 26.0 37.0 82-83 32.616533551799 35.0 33.0 35.0 25.5 37.0 84-85 32.480852666648744 35.0 33.0 35.0 25.0 36.0 86-87 32.34861834053409 35.0 33.0 35.0 25.0 36.0 88-89 32.25778620057959 35.0 33.0 35.0 25.0 36.0 90-91 32.12245628156744 35.0 32.0 35.0 25.0 36.0 92-93 31.973059960672707 35.0 32.0 35.0 25.0 35.0 94-95 31.93113268059631 35.0 32.0 35.0 25.0 35.0 96-97 31.819312505366952 35.0 32.0 35.0 24.0 35.0 98-99 31.72928909461362 35.0 32.0 35.0 24.0 35.0 100 30.746023034307726 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2732968754025116 1101 2 0.33409768084874614 1101 3 0.39838485113207156 1101 4 0.4316821367620207 1101 5 0.3389197171923968 1101 6 0.3224398594638487 1101 7 0.34388080765974394 1101 8 0.3392053347498063 1101 9 0.45214611033906493 1101 10-11 0.5180794192442875 1101 12-13 0.4572485219129021 1101 14-15 0.6791092610427825 1101 16-17 0.5925486812895926 1101 18-19 0.5977250792064339 1101 20-21 0.5843281093642574 1101 22-23 0.5868266046729147 1101 24-25 0.6476539554584804 1101 26-27 0.6383138137651443 1101 28-29 0.5762132230077412 1101 30-31 0.5786618270756776 1101 32-33 0.5378903944962303 1101 34-35 0.5909438773537872 1101 36-37 0.5600568635905034 1101 38-39 0.5496946073241276 1101 40-41 0.5620096489696635 1101 42-43 0.5387977165441953 1101 44-45 0.5331792980309302 1101 46-47 0.5990436505893229 1101 48-49 0.5329133898937499 1101 50-51 0.5167418550358036 1101 52-53 0.488271971927972 1101 54-55 0.4743524581538665 1101 56-57 0.4239199871798718 1101 58-59 0.3925504388405159 1101 60-61 0.4037485232773932 1101 62-63 0.39357068738561907 1101 64-65 0.43233743177192707 1101 66-67 0.46567727715949303 1101 68-69 0.4093465892647785 1101 70-71 0.4525885257702882 1101 72-73 0.4280526658361481 1101 74-75 0.41717943421846115 1101 76-77 0.44189085859530053 1101 78-79 0.3697526657701218 1101 80-81 0.40044950483193986 1101 82-83 0.4200897616676116 1101 84-85 0.4182516557810203 1101 86-87 0.4282947212022634 1101 88-89 0.44701861050588576 1101 90-91 0.47527776926250453 1101 92-93 0.42164921863311733 1101 94-95 0.4599322517833144 1101 96-97 0.4001174425616618 1101 98-99 0.3390031787287313 1101 100 0.45490959942381437 1102 1 0.274922602614172 1102 2 0.3824504053047484 1102 3 0.39303766989098676 1102 4 0.4417045397201136 1102 5 0.32647805052424417 1102 6 0.3504396136809689 1102 7 0.3151059640277367 1102 8 0.3705851605558692 1102 9 0.49290801588180244 1102 10-11 0.5788882137977822 1102 12-13 0.5145705837514498 1102 14-15 0.7004723093600518 1102 16-17 0.6084060103871636 1102 18-19 0.7060112974705461 1102 20-21 0.6286526079110359 1102 22-23 0.6510367250637472 1102 24-25 0.6694523482630075 1102 26-27 0.5960842734076479 1102 28-29 0.644984150867522 1102 30-31 0.6329255830302074 1102 32-33 0.5849392688409338 1102 34-35 0.5629547725399533 1102 36-37 0.6393243156454602 1102 38-39 0.640302047505223 1102 40-41 0.6098925520522513 1102 42-43 0.6070046938906444 1102 44-45 0.5888238337820013 1102 46-47 0.5423035630449959 1102 48-49 0.5089843589431098 1102 50-51 0.48240665272476235 1102 52-53 0.3947381737519038 1102 54-55 0.44284252248339584 1102 56-57 0.4385679294923648 1102 58-59 0.4039763239841534 1102 60-61 0.46500432541984793 1102 62-63 0.4591849471950553 1102 64-65 0.47793868906097714 1102 66-67 0.517111243818924 1102 68-69 0.42316210693361 1102 70-71 0.4127387817213446 1102 72-73 0.4164565016353592 1102 74-75 0.3619441430049619 1102 76-77 0.37352793878379487 1102 78-79 0.39394118123973954 1102 80-81 0.44162747231266763 1102 82-83 0.4228166661418342 1102 84-85 0.4236522511316849 1102 86-87 0.3650390814297779 1102 88-89 0.4817057221257173 1102 90-91 0.4788747054781979 1102 92-93 0.48637820780601615 1102 94-95 0.51061447751038 1102 96-97 0.5346359260046505 1102 98-99 0.4145679468845316 1102 100 0.48006353454319495 1103 1 0.31969045472195745 1103 2 0.37713960809118063 1103 3 0.30327260163998204 1103 4 0.3904527702892935 1103 5 0.2717140772091966 1103 6 0.2879770955572738 1103 7 0.35849888430065846 1103 8 0.348361181029027 1103 9 0.3793352470106157 1103 10-11 0.5059100166205681 1103 12-13 0.38214604129871077 1103 14-15 0.5508882240037138 1103 16-17 0.5725422955628758 1103 18-19 0.6315374929187598 1103 20-21 0.5287209161705775 1103 22-23 0.5816120602358126 1103 24-25 0.6173625592206662 1103 26-27 0.5102740351123671 1103 28-29 0.5246455007732678 1103 30-31 0.5401270770094939 1103 32-33 0.5310246759925192 1103 34-35 0.5472622112342762 1103 36-37 0.5762762214781105 1103 38-39 0.5710037833723192 1103 40-41 0.5864996568268879 1103 42-43 0.6220598843880367 1103 44-45 0.5156856006221915 1103 46-47 0.4706159411170745 1103 48-49 0.5201756063256369 1103 50-51 0.48009932567097025 1103 52-53 0.44177766014821884 1103 54-55 0.42109068936022 1103 56-57 0.46330185289837544 1103 58-59 0.4366917401099215 1103 60-61 0.40590256293078397 1103 62-63 0.4489413024752906 1103 64-65 0.4357285500971386 1103 66-67 0.4184599293948281 1103 68-69 0.44422754760596916 1103 70-71 0.4469143808230669 1103 72-73 0.3706345053023199 1103 74-75 0.3201611543021272 1103 76-77 0.2401465797464155 1103 78-79 0.3202778439894445 1103 80-81 0.2975131466858727 1103 82-83 0.41423394265682134 1103 84-85 0.4019328009183454 1103 86-87 0.3805800155747505 1103 88-89 0.39773898384689943 1103 90-91 0.4141836706420605 1103 92-93 0.39555237561890166 1103 94-95 0.33241043011182114 1103 96-97 0.4407354170383364 1103 98-99 0.4063196082530425 1103 100 0.5044866893614142 1104 1 0.20174968466905696 1104 2 0.34916009346951427 1104 3 0.3147433247639029 1104 4 0.3825005601040843 1104 5 0.2782344971577828 1104 6 0.2963303585394712 1104 7 0.3081601123862896 1104 8 0.34628823185733637 1104 9 0.3632051071626421 1104 10-11 0.47656818159566683 1104 12-13 0.4180614853109006 1104 14-15 0.5435768442909961 1104 16-17 0.5372902255041794 1104 18-19 0.5019317469785136 1104 20-21 0.5316147261489377 1104 22-23 0.49752521552082385 1104 24-25 0.5493562455569574 1104 26-27 0.5060255688573818 1104 28-29 0.4112245727500152 1104 30-31 0.4222956302483709 1104 32-33 0.49163038637121304 1104 34-35 0.5323040148844314 1104 36-37 0.450203002879519 1104 38-39 0.4695989905691391 1104 40-41 0.5190001061636877 1104 42-43 0.5053921038683029 1104 44-45 0.47512376890118446 1104 46-47 0.46314404174680135 1104 48-49 0.447788893941663 1104 50-51 0.40443365904874184 1104 52-53 0.42813890888757555 1104 54-55 0.4516939629846064 1104 56-57 0.40876029292365246 1104 58-59 0.3908319056865821 1104 60-61 0.3893047799742888 1104 62-63 0.4277705322454608 1104 64-65 0.41971996329726835 1104 66-67 0.3928567648200172 1104 68-69 0.41965679562989067 1104 70-71 0.4027476067126443 1104 72-73 0.38228302521494584 1104 74-75 0.32944914511497814 1104 76-77 0.24164815733024625 1104 78-79 0.33540620584763303 1104 80-81 0.286202187755606 1104 82-83 0.3691101381072954 1104 84-85 0.3856648929201043 1104 86-87 0.3607869815914029 1104 88-89 0.31034777283957027 1104 90-91 0.3991424280059732 1104 92-93 0.37003387774568 1104 94-95 0.40584220090345724 1104 96-97 0.4077809645486532 1104 98-99 0.39041493606131894 1104 100 0.4085178973346295 1105 1 0.15356033418991544 1105 2 0.2449258273475401 1105 3 0.2503473579690798 1105 4 0.3529490278587062 1105 5 0.21313924895900982 1105 6 0.2672706181680198 1105 7 0.2471820887964995 1105 8 0.2855793626787886 1105 9 0.36159870398703475 1105 10-11 0.4408325677513858 1105 12-13 0.33290748500914447 1105 14-15 0.49971233177592467 1105 16-17 0.5208806083169932 1105 18-19 0.5052954341685876 1105 20-21 0.4765579551437682 1105 22-23 0.49367792954848255 1105 24-25 0.4786808621837224 1105 26-27 0.45193091858920553 1105 28-29 0.5024809610655794 1105 30-31 0.509109154677617 1105 32-33 0.4352665386419474 1105 34-35 0.49013081858169016 1105 36-37 0.4455680863210034 1105 38-39 0.4577619956152006 1105 40-41 0.42748969073204535 1105 42-43 0.4631766213066584 1105 44-45 0.4674101794905994 1105 46-47 0.499651839000677 1105 48-49 0.3629195468214732 1105 50-51 0.4119418433178481 1105 52-53 0.44881646642252804 1105 54-55 0.362434305398466 1105 56-57 0.4573727330044406 1105 58-59 0.4356186259934418 1105 60-61 0.46114529527476833 1105 62-63 0.41257772331167786 1105 64-65 0.39461553594659904 1105 66-67 0.4060988646726784 1105 68-69 0.4451026602721413 1105 70-71 0.4263562788999664 1105 72-73 0.3640179513760984 1105 74-75 0.3363772502404814 1105 76-77 0.33860324331777036 1105 78-79 0.3613436353511901 1105 80-81 0.3335150012535806 1105 82-83 0.30140325423661096 1105 84-85 0.3032166250191324 1105 86-87 0.393435832679522 1105 88-89 0.34561461595281884 1105 90-91 0.3600361618118484 1105 92-93 0.3986412026131312 1105 94-95 0.36860672503125613 1105 96-97 0.33441016752985675 1105 98-99 0.24658245708996418 1105 100 0.37835345166651635 1106 1 0.09655721536247341 1106 2 0.21154116675089085 1106 3 0.21137522984290058 1106 4 0.3030111180524955 1106 5 0.19687087768048883 1106 6 0.2460003497383738 1106 7 0.25668899611638807 1106 8 0.22803339039188586 1106 9 0.34339877565062693 1106 10-11 0.38657266683021874 1106 12-13 0.31142778748038324 1106 14-15 0.403956636787413 1106 16-17 0.35711659469595247 1106 18-19 0.4216153138979095 1106 20-21 0.3291375253016682 1106 22-23 0.31139506807659245 1106 24-25 0.42350927310607744 1106 26-27 0.3889584993026176 1106 28-29 0.3691799106153866 1106 30-31 0.49511636034633 1106 32-33 0.36170411461021246 1106 34-35 0.357526473398579 1106 36-37 0.3055665060510293 1106 38-39 0.37706843592039974 1106 40-41 0.4166195445330487 1106 42-43 0.4099007555229406 1106 44-45 0.47188768658084257 1106 46-47 0.4095550131966519 1106 48-49 0.35343549472723623 1106 50-51 0.27804032375750154 1106 52-53 0.26875303347192414 1106 54-55 0.4235292087638953 1106 56-57 0.389577713399369 1106 58-59 0.32668930104719607 1106 60-61 0.38698553352528364 1106 62-63 0.3784198746656955 1106 64-65 0.32430781027375133 1106 66-67 0.3160539628689989 1106 68-69 0.35110608896956563 1106 70-71 0.3396737539956476 1106 72-73 0.28429684657517384 1106 74-75 0.2651768610970322 1106 76-77 0.26905035061427895 1106 78-79 0.32406936196913705 1106 80-81 0.3270490009078273 1106 82-83 0.33612043061715013 1106 84-85 0.3439298959428285 1106 86-87 0.36693265305580525 1106 88-89 0.4184313550137304 1106 90-91 0.3515767840262072 1106 92-93 0.3648472257026434 1106 94-95 0.4163631276750692 1106 96-97 0.4107282168716999 1106 98-99 0.37217596919153095 1106 100 0.2663107939880085 1107 1 0.16610940990225487 1107 2 0.18026402439853229 1107 3 0.12480635518817351 1107 4 0.22802393146606903 1107 5 0.11079560476737527 1107 6 0.18745831610956998 1107 7 0.12085491774246293 1107 8 0.14163237219609925 1107 9 0.13827314342834995 1107 10-11 0.27284096726022966 1107 12-13 0.1457343239072344 1107 14-15 0.2749756411029125 1107 16-17 0.21256802407707198 1107 18-19 0.15621511371518437 1107 20-21 0.12310163307010669 1107 22-23 0.29945100644533085 1107 24-25 0.30947858153449204 1107 26-27 0.22675437502866203 1107 28-29 0.2538476274533181 1107 30-31 0.36588339470139886 1107 32-33 0.3211066034212706 1107 34-35 0.2883761894728991 1107 36-37 0.33502486994189695 1107 38-39 0.35400716319937686 1107 40-41 0.24965515189032317 1107 42-43 0.3261318229447667 1107 44-45 0.31137780090998746 1107 46-47 0.3229273146724836 1107 48-49 0.31132503361628494 1107 50-51 0.2542933036728243 1107 52-53 0.25956155838642303 1107 54-55 0.22850195821267505 1107 56-57 0.16897680876792265 1107 58-59 0.17484064191275195 1107 60-61 0.1671777784531301 1107 62-63 0.25183487245132596 1107 64-65 0.2297732573366318 1107 66-67 0.21204976742094317 1107 68-69 0.1944708898925427 1107 70-71 0.24573509148777362 1107 72-73 0.24588376672559775 1107 74-75 0.21032787336410763 1107 76-77 0.22738483880993243 1107 78-79 0.19359047897864912 1107 80-81 0.1503689986074832 1107 82-83 0.12151109373686353 1107 84-85 0.08477986621037559 1107 86-87 0.18365650331687533 1107 88-89 0.26514711555689274 1107 90-91 0.17956676703454377 1107 92-93 0.09516062742160969 1107 94-95 0.11092636442782577 1107 96-97 0.1944761470125833 1107 98-99 0.13202714239331215 1107 100 0.19064288090993742 1108 1 0.028967004216244874 1108 2 0.06717243890965108 1108 3 0.016584231042482855 1108 4 0.1689333316562056 1108 5 0.08332067413739708 1108 6 0.08618525126518506 1108 7 0.14767421303984207 1108 8 0.11399852271441802 1108 9 0.09290838728522033 1108 10-11 0.24024617653374492 1108 12-13 0.11998204061043793 1108 14-15 0.19954651568305337 1108 16-17 0.19836112022811392 1108 18-19 0.1515732344814964 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-1.4675008596025023 2215 34-35 -1.1503228830840015 2215 36-37 -1.0046286619433573 2215 38-39 -0.8847185909603894 2215 40-41 -0.9021414179498706 2215 42-43 -0.9101212313192022 2215 44-45 -0.8664008990222953 2215 46-47 -1.0408017121756217 2215 48-49 -1.0797988950747097 2215 50-51 -1.0315532606368976 2215 52-53 -1.030820345220917 2215 54-55 -0.9301686544581145 2215 56-57 -0.9357008814407237 2215 58-59 -0.9639662050513351 2215 60-61 -0.933985890234915 2215 62-63 -0.9380554402735299 2215 64-65 -0.9995469376361612 2215 66-67 -0.8866434444498097 2215 68-69 -0.8430505921807736 2215 70-71 -0.9670705849570496 2215 72-73 -0.8607283948490121 2215 74-75 -0.9861393846025663 2215 76-77 -0.8852883231747555 2215 78-79 -0.8752921641827172 2215 80-81 -0.8221960183104713 2215 82-83 -0.8502218513649282 2215 84-85 -0.8710591760642288 2215 86-87 -0.8633165578248878 2215 88-89 -0.8617159450141649 2215 90-91 -0.9443503224585541 2215 92-93 -0.9768762805601021 2215 94-95 -0.9053228273284759 2215 96-97 -1.1170624180525799 2215 98-99 -1.0079682052412053 2215 100 -0.5866982605919837 2216 1 -0.561508049465747 2216 2 -0.7889364529385752 2216 3 -0.7309712250916327 2216 4 -0.5975982366991062 2216 5 -0.5275420575666061 2216 6 -0.4886745877506442 2216 7 -0.6174667285635991 2216 8 -0.6548994284699248 2216 9 -0.8098152423882752 2216 10-11 -1.1935031591199703 2216 12-13 -0.7339179888015366 2216 14-15 -0.873151889103795 2216 16-17 -0.903306521852258 2216 18-19 -1.1899516638316712 2216 20-21 -1.3074129324523014 2216 22-23 -1.1573913387713048 2216 24-25 -1.2035714897233731 2216 26-27 -1.4966816230261912 2216 28-29 -1.3968746656680366 2216 30-31 -1.583520631340015 2216 32-33 -1.774254150881255 2216 34-35 -1.379843313234396 2216 36-37 -1.5000280622515376 2216 38-39 -1.2934331363838965 2216 40-41 -1.2265528155950136 2216 42-43 -1.2422303123330352 2216 44-45 -1.1803336391220967 2216 46-47 -1.2839008483585062 2216 48-49 -1.3188143801716024 2216 50-51 -1.3484027489356407 2216 52-53 -1.2789480496726284 2216 54-55 -1.3836597828527601 2216 56-57 -1.2894677764835123 2216 58-59 -1.1595315382892295 2216 60-61 -1.2232963632378855 2216 62-63 -1.3426026590921794 2216 64-65 -1.3600196084079812 2216 66-67 -1.2962392350764773 2216 68-69 -1.311119567670218 2216 70-71 -1.2366529067721288 2216 72-73 -1.1870455761103003 2216 74-75 -1.1884398023264708 2216 76-77 -1.1984118665331067 2216 78-79 -1.045657771011868 2216 80-81 -1.1036190065063138 2216 82-83 -1.0966202677133055 2216 84-85 -1.196137263094343 2216 86-87 -1.2277212099908432 2216 88-89 -1.186445059586795 2216 90-91 -1.2966167020172819 2216 92-93 -1.2114040252189255 2216 94-95 -1.2984054641635794 2216 96-97 -1.268946594274329 2216 98-99 -1.3972320235610596 2216 100 -0.9956535450376691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 24.0 4 59.0 5 20.0 6 55.0 7 163.0 8 469.0 9 741.0 10 1147.0 11 1943.0 12 3300.0 13 5049.0 14 7151.0 15 9716.0 16 12023.0 17 14268.0 18 16233.0 19 18536.0 20 21313.0 21 24891.0 22 28404.0 23 33337.0 24 39123.0 25 45499.0 26 51568.0 27 58399.0 28 68056.0 29 82575.0 30 96166.0 31 115215.0 32 144150.0 33 186517.0 34 270536.0 35 402061.0 36 667006.0 37 1161401.0 38 789935.0 39 234371.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84854478053183 20.390452519172747 11.588813030851066 24.172189669444354 2 16.38221088943601 19.679810236070715 38.9634982454687 24.974480629024573 3 19.035131796011388 26.475354784535583 28.391545849770743 26.09796756968229 4 12.523259132543874 16.122319832265326 39.98974646352751 31.364674571663286 5 13.297147491640246 38.885431259189225 34.48928520312791 13.328136046042616 6 29.97658216744737 38.33227473482812 17.716471828883062 13.974671268841446 7 27.083158499401172 31.52984949210878 23.624515045910037 17.76247696258001 8 27.15895392391295 33.892213802897594 19.389996402457257 19.5588358707322 9 26.57756382107871 14.347878413323143 21.240392278042513 37.83416548755564 10-11 26.67977656432135 24.950198182663296 26.635311914476944 21.734713338538405 12-13 26.54706386714032 22.961321831179244 26.753198821565544 23.738415480114895 14-15 23.990727196850603 24.05191052336953 24.205232058068106 27.752130221711763 16-17 22.917842614821513 26.631005304750467 25.569816678943873 24.88133540148415 18-19 22.0429619031283 27.892362562048937 27.07574855019461 22.98892698462815 20-21 24.26213056110659 27.007742702569782 25.905943690598953 22.824183045724673 22-23 25.011590623933373 26.2786008674861 25.658572963248723 23.051235545331807 24-25 24.391079036690357 26.157880384842453 26.566966587317893 22.884073991149297 26-27 24.185712756887607 26.638341086436284 25.088512016848753 24.087434139827355 28-29 22.89365304706025 27.159970026160423 26.241357067646824 23.705019859132495 30-31 23.718472722848816 26.902083781281416 26.313996397331817 23.065447098537952 32-33 24.23368475610219 26.897948606182048 25.054155539001854 23.81421109871391 34-35 24.14442732497937 26.781097219165172 26.238613778723746 22.835861677131707 36-37 23.77478723668415 26.091998156215716 25.525796769300452 24.60741783779968 38-39 24.060307233096765 26.36695719136734 26.269369864534692 23.303365711001202 40-41 23.514871936553693 25.212116230911708 27.24325993560558 24.02975189692902 42-43 23.559417019691992 25.563382007560847 26.359578551415257 24.517622421331904 44-45 23.923287132866026 26.339200652063276 26.31398698931624 23.42352522575446 46-47 23.71696555906349 26.35040546784881 25.630281034510993 24.30234793857671 48-49 24.666641506716623 26.0225025387691 25.846686543027204 23.464169411487077 50-51 23.86372373520644 25.578845647703417 26.33212117952221 24.225309437567933 52-53 23.497991422129093 26.1777111788548 25.945397004978904 24.3789003940372 54-55 23.258908668880895 26.13975882500362 25.901429423340883 24.6999030827746 56-57 24.77700039244591 25.535378527038482 25.37594940950391 24.311671671011695 58-59 23.010721880993856 26.20069592296113 26.76908975979087 24.019492436254144 60-61 23.23228796147119 26.762317076913284 25.84640983468428 24.15898512693125 62-63 23.88493073324002 26.066132091552962 25.20543203729158 24.843505137915432 64-65 22.799218132083453 26.33720732273565 25.96465328955116 24.898921255629734 66-67 23.863150854214645 25.902795158084036 25.83341610817146 24.400637879529864 68-69 23.02103949566538 25.755266379669 26.411078776315634 24.812615348349983 70-71 22.892798743911484 25.652842897851723 26.214320103054778 25.240038255182018 72-73 23.6451456900216 25.5992309961051 26.87585119579455 23.879772118078748 74-75 23.955429731137055 25.835310303965876 26.286241780815526 23.92301818408154 76-77 23.609479158940648 25.865617793114875 25.652954364761804 24.871948683182673 78-79 23.646041543748762 25.914002269153535 25.510822504719993 24.929133682377714 80-81 23.0972587496484 26.506304066885978 26.421779235236237 23.974657948229382 82-83 24.068771419097697 25.173244894493834 27.768594915865492 22.98938877054298 84-85 24.089946975200878 25.666963056136886 26.42304898016721 23.820040988495027 86-87 22.461300942405288 26.603022059793847 26.884211482812283 24.051465514988582 88-89 24.04713714463295 24.863088279081527 26.620956054010435 24.468818522275086 90-91 23.453444612342516 25.244373019991073 26.32642095714912 24.975761410517286 92-93 23.868935642906784 25.365469753832798 26.72162687240256 24.043967730857858 94-95 22.679689009570822 25.349003286127285 27.612956965893716 24.358350738408177 96-97 23.081272938481955 25.2567250340238 26.96892646576134 24.693075561732904 98-99 22.398261508285344 25.94734233424891 26.757296784612254 24.897099372853493 100 23.389753270846793 24.987167075008422 25.725769578716978 25.897310075427814 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 282.0 1 196.0 2 130.5 3 152.5 4 151.0 5 139.5 6 137.0 7 143.5 8 154.5 9 159.0 10 145.5 11 133.0 12 130.5 13 145.0 14 155.0 15 178.0 16 201.0 17 223.5 18 299.5 19 365.5 20 422.5 21 540.0 22 755.5 23 1081.0 24 1474.0 25 1983.0 26 2813.5 27 3681.5 28 5301.5 29 9511.0 30 16695.0 31 24622.0 32 36498.0 33 49935.5 34 60925.0 35 71927.0 36 83466.0 37 104528.0 38 118031.5 39 120362.5 40 116205.5 41 107015.5 42 107381.5 43 118277.5 44 137035.0 45 172908.5 46 210090.0 47 237054.5 48 253650.5 49 249001.0 50 235639.0 51 244475.5 52 251839.5 53 240917.0 54 229048.0 55 195697.5 56 170089.5 57 138498.0 58 104990.0 59 102868.5 60 85086.5 61 55996.0 62 40824.5 63 34737.5 64 25360.5 65 11000.5 66 5219.0 67 3535.5 68 2549.0 69 1859.5 70 1407.0 71 1015.0 72 708.0 73 491.5 74 319.5 75 200.0 76 129.5 77 73.0 78 50.0 79 43.0 80 34.5 81 21.5 82 14.0 83 11.0 84 11.0 85 12.5 86 12.5 87 10.0 88 8.5 89 8.0 90 10.0 91 8.5 92 7.0 93 8.0 94 6.5 95 6.5 96 7.5 97 6.5 98 5.0 99 4.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060738718124641655 2 0.0076546831481608376 3 0.008175114863616531 4 0.010061679832143424 5 0.0038164992466750924 6 0.002450365993603894 7 8.023322279941955E-4 8 0.0016263491107990449 9 6.722242991302719E-4 10-11 5.963280072929831E-4 12-13 3.0358516734915503E-4 14-15 1.1926560145859664E-4 16-17 6.28854989508964E-4 18-19 0.0016263491107990449 20-21 0.0011384443775593314 22-23 0.0015179258367457751 24-25 0.001268552306423255 26-27 0.0038273415740804188 28-29 0.004369457944346768 30-31 0.005453690684879464 32-33 0.006527081098006832 34-35 0.003708075972621822 36-37 0.0084028037391284 38-39 0.0065379234254121605 40-41 0.0062234959306576776 42-43 0.015114204403025789 44-45 0.0208281109456331 46-47 0.01939692372812994 48-49 0.022855626170429244 50-51 0.019353554418508633 52-53 0.026119166719432662 54-55 0.019960724753206944 56-57 0.01581895568437204 58-59 0.018312690987597247 60-61 0.01675139584123016 62-63 0.01175308290737443 64-65 0.0118615061814277 66-67 0.01418176424616767 68-69 0.005475375339690117 70-71 0.009020816401232035 72-73 0.008587123305018957 74-75 0.011818136871806393 76-77 0.01114591257267612 78-79 0.012696365391637876 80-81 0.010517057583167157 82-83 0.006700558336492065 84-85 0.01097243533419089 86-87 0.009313559241175863 88-89 0.008088376244373916 90-91 0.013520382274442728 92-93 0.008706388906477553 94-95 0.0076546831481608376 96-97 0.009660513718146326 98-99 0.009378613205607825 100 0.26149525236167576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4611556.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.00561967502189 #Duplication Level Percentage of deduplicated Percentage of total 1 73.86308823633027 48.0151582191347 2 15.165444995166805 19.716782991165548 3 5.403647424888377 10.538023480806158 4 2.4102891016276184 6.267293465890207 5 1.18333628952363 3.846175439221234 6 0.684943496193963 2.6715105847478737 7 0.4051861944075951 1.8437565755860728 8 0.24198512246753232 1.25843142705104 9 0.17220443070482 1.0074830155876073 >10 0.4639531054216482 4.266026754373104 >50 0.003996743321208254 0.1740579342936892 >100 0.0017779217854548325 0.22655153739106465 >500 4.8979388502678256E-5 0.01611071363465643 >1k 9.795877264315765E-5 0.1526378611170049 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1684654810653933E-5 2 0.0 0.0 0.0 0.0 2.1684654810653933E-5 3 0.0 0.0 0.0 0.0 2.1684654810653933E-5 4 0.0 0.0 0.0 0.0 2.1684654810653933E-5 5 0.0 0.0 0.0 0.0 2.1684654810653933E-5 6 0.0 0.0 0.0 0.0 2.1684654810653933E-5 7 0.0 0.0 0.0 0.0 2.1684654810653933E-5 8 0.0 0.0 0.0 0.0 2.1684654810653933E-5 9 0.0 0.0 0.0 0.0 2.1684654810653933E-5 10-11 0.0 0.0 0.0 0.0 2.1684654810653933E-5 12-13 0.0 0.0 0.0 0.0 1.1926560145859662E-4 14-15 0.0 0.0 0.0 0.0 1.7347723848523146E-4 16-17 0.0 0.0 0.0 0.0 2.3853120291719324E-4 18-19 0.0 0.0 0.0 0.0 2.602158577278472E-4 20-21 0.0 0.0 0.0 1.0842327405326966E-5 3.0358516734915503E-4 22-23 0.0 0.0 0.0 6.505396443196178E-5 3.0358516734915503E-4 24-25 0.0 0.0 0.0 2.2768887551186627E-4 3.25269822159809E-4 26-27 0.0 0.0 0.0 6.28854989508964E-4 3.686391317811168E-4 28-29 0.0 0.0 0.0 0.0036538643355951876 3.686391317811168E-4 30-31 0.0 0.0 0.0 0.013130058487850956 3.794814591864438E-4 32-33 0.0 0.0 0.0 0.030466940008968775 4.336930962130786E-4 34-35 0.0 0.0 0.0 0.05894973410276271 4.770624058343865E-4 36-37 0.0 0.0 0.0 0.1079570539748406 4.770624058343865E-4 38-39 0.0 0.0 0.0 0.18545801026811776 4.987470606450404E-4 40-41 0.0 0.0 0.0 0.2709280772043102 4.987470606450404E-4 42-43 0.0 0.0 0.0 0.36110371423441456 5.095893880503674E-4 44-45 0.0 0.0 0.0 0.47048111309935303 5.312740428610214E-4 46-47 0.0 0.0 0.0 0.6065197950539905 5.963280072929832E-4 48-49 0.0 0.0 0.0 0.7629962641676693 6.722242991302719E-4 50-51 0.0 0.0 0.0 0.9357036974071224 6.939089539409258E-4 52-53 0.0 0.0 0.0 1.104442838816226 6.939089539409258E-4 54-55 0.0 0.0 0.0 1.2896840025362373 7.155936087515797E-4 56-57 0.0 0.0 0.0 1.5147490348160144 7.914899005888685E-4 58-59 0.0 0.0 0.0 1.7928547327626512 8.240168828048494E-4 60-61 0.0 0.0 0.0 2.076164747863845 8.565438650208303E-4 62-63 0.0 0.0 0.0 2.37462149435028 8.890708472368111E-4 64-65 0.0 0.0 0.0 2.6887454039374132 8.999131746421381E-4 66-67 0.0 0.0 0.0 3.0430509788886875 9.32440156858119E-4 68-69 0.0 0.0 0.0 3.4437291881525454 9.54124811668773E-4 70-71 0.0 0.0 0.0 3.8870177441193388 9.866517938847538E-4 72-73 0.0 0.0 0.0 4.349735750796477 0.0010517057583167156 74-75 0.0 0.0 0.0 4.800028450267112 0.0011167597227486775 76-77 0.0 0.0 0.0 5.320373427103563 0.0011709713597753122 78-79 0.0 0.0 0.0 5.874004349074369 0.001181813687180639 80-81 0.0 0.0 0.0 6.4737260048452185 0.0012034983419912933 82-83 0.0 0.0 0.0 7.09648760635239 0.001257709979017928 84-85 0.0 0.0 0.0 7.701934010993252 0.001257709979017928 86-87 0.0 0.0 0.0 8.346759748770264 0.0013119216160445628 88 0.0 0.0 0.0 8.862605159733505 0.0013878179078818517 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5600 0.0 43.07306 94 GTACTAG 6925 0.0 35.511654 1 TATTCTC 1410 0.0 35.32857 5 GTACCGT 135 3.4742698E-10 34.808613 6 TAGGCAT 8595 0.0 31.38379 5 TACCCCG 315 0.0 31.329113 5 CAGTACG 765 0.0 30.713152 9 GCATCGT 7460 0.0 30.298565 8 CTGTGCG 8620 0.0 30.037247 9 TAGTACT 8650 0.0 29.990973 4 ACTGTGC 8985 0.0 28.973934 8 CTAGGCA 9780 0.0 28.2557 4 AACGCGT 100 5.3338255E-5 28.194672 8 CAACGCA 9390 0.0 28.076088 5 CTAACGC 3480 0.0 27.954948 3 TAACGCC 3550 0.0 27.933266 4 TCTAACG 3500 0.0 27.929785 2 CGCCTAC 3550 0.0 27.797565 7 GTACTGT 9340 0.0 27.722044 6 TATTCGC 715 0.0 27.603174 9 >>END_MODULE