FastQCFastQC Report
Mon 16 Jan 2017
ERR1146664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4611556
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.1317559626295333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51410.11148081038157186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG2200.046.999165
GGGCACC60550.044.1928594
CAGTACG9050.033.7606969
GTACTAG64150.033.5860821
GTATCAA160100.033.438091
GGTATCA103650.032.995571
CTGTGCG88600.032.256449
TAGTACT89600.031.9178984
TATTCTC15650.031.5329825
ACTGTGC93250.031.147638
GAACCGT6800.031.1010386
TAGGCAT80900.030.906745
CCGTGTT6800.030.4151789
GTACTGT95900.030.0409156
CAACGCA166350.029.5528245
CTAACGC31100.029.3413473
TGAACCG7550.029.2577535
TACTGTG105800.028.829367
TCTAACG32650.028.6721612
ATCAACG171950.028.6680283
Produced by FastQC (version 0.11.5)