##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146664_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4611556 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.101650072123164 33.0 31.0 34.0 30.0 34.0 2 32.179593612221126 33.0 31.0 34.0 30.0 34.0 3 32.35012976097439 34.0 31.0 34.0 30.0 34.0 4 35.95461380063475 37.0 35.0 37.0 35.0 37.0 5 35.85348828031146 37.0 35.0 37.0 35.0 37.0 6 35.888902140622385 37.0 35.0 37.0 35.0 37.0 7 35.8269831267364 37.0 35.0 37.0 35.0 37.0 8 35.83053225418926 37.0 35.0 37.0 35.0 37.0 9 37.53833239800189 39.0 37.0 39.0 35.0 39.0 10-11 37.481210246606565 39.0 37.0 39.0 34.5 39.0 12-13 37.436448782146414 39.0 37.0 39.0 34.0 39.0 14-15 38.66085720307853 40.0 38.0 41.0 34.0 41.0 16-17 38.65406567761511 40.0 38.0 41.0 34.0 41.0 18-19 38.69238831752233 40.0 38.0 41.0 34.0 41.0 20-21 38.68424529594783 40.0 38.0 41.0 34.0 41.0 22-23 38.637718917432636 40.0 38.0 41.0 34.0 41.0 24-25 38.55308663713505 40.0 38.0 41.0 34.0 41.0 26-27 38.41050688748006 40.0 38.0 41.0 34.0 41.0 28-29 38.354792395451774 40.0 38.0 41.0 34.0 41.0 30-31 38.24275266309246 40.0 37.5 41.0 33.5 41.0 32-33 38.12435336359355 40.0 37.0 41.0 33.0 41.0 34-35 38.03114079065721 40.0 37.0 41.0 33.0 41.0 36-37 38.02440466948683 40.0 37.0 41.0 33.0 41.0 38-39 37.92587794661932 40.0 37.0 41.0 33.0 41.0 40-41 37.80954584526351 40.0 37.0 41.0 33.0 41.0 42-43 37.66615866748664 40.0 36.5 41.0 32.5 41.0 44-45 37.442827106512425 40.0 36.0 41.0 32.0 41.0 46-47 37.21142430017113 39.5 35.5 41.0 31.5 41.0 48-49 36.93733568452817 39.0 35.0 41.0 31.0 41.0 50-51 36.69636983699211 39.0 35.0 41.0 31.0 41.0 52-53 36.4757268479446 39.0 35.0 41.0 30.5 41.0 54-55 36.24284438918231 38.0 35.0 40.0 30.0 41.0 56-57 35.9746704799855 37.5 35.0 40.0 30.0 41.0 58-59 35.65034318134703 37.0 35.0 40.0 30.0 41.0 60-61 35.446104308393956 36.0 34.0 39.5 30.0 41.0 62-63 35.18816815842635 36.0 34.0 39.0 29.5 41.0 64-65 34.914338132292 35.0 34.0 39.0 29.0 41.0 66-67 34.6494169213168 35.0 34.0 38.5 29.0 41.0 68-69 34.38924356551238 35.0 34.0 37.0 29.0 40.0 70-71 34.20013006455955 35.0 34.0 37.0 29.0 40.0 72-73 33.98062302615429 35.0 34.0 36.0 29.0 39.5 74-75 33.750267697063634 35.0 34.0 36.0 29.0 39.0 76-77 33.54606102582295 35.0 33.0 35.5 29.0 39.0 78-79 33.360234051153235 35.0 33.0 35.0 29.0 37.5 80-81 33.272161934063035 35.0 33.0 35.0 29.0 37.0 82-83 33.15066790905283 35.0 33.0 35.0 29.0 37.0 84-85 33.06770946726007 35.0 33.0 35.0 29.0 36.0 86-87 32.973547106443036 35.0 33.0 35.0 29.0 36.0 88-89 32.904991287105695 35.0 33.0 35.0 29.0 36.0 90-91 32.86154261164778 35.0 33.0 35.0 29.0 36.0 92-93 32.808805639571545 35.0 33.0 35.0 29.0 35.0 94-95 32.788122815813146 35.0 33.0 35.0 29.0 35.0 96-97 32.7074655495889 35.0 33.0 35.0 29.0 35.0 98-99 32.636531899428306 35.0 33.0 35.0 27.5 35.0 100 31.354419419389032 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32153786230176706 1101 2 0.413800478113842 1101 3 0.33558799426092634 1101 4 0.24977291276025682 1101 5 0.23530598446719608 1101 6 0.2669411891290423 1101 7 0.28291964561475424 1101 8 0.30463627314257735 1101 9 0.36351977055259255 1101 10-11 0.3913960604895905 1101 12-13 0.40478499081118 1101 14-15 0.5366197284286898 1101 16-17 0.5305760535699591 1101 18-19 0.5089349489724597 1101 20-21 0.5174140058005108 1101 22-23 0.48473972109790964 1101 24-25 0.43190621501140924 1101 26-27 0.5552942769752534 1101 28-29 0.5132386600059817 1101 30-31 0.5814400396220663 1101 32-33 0.5862076611327467 1101 34-35 0.5715904322326111 1101 36-37 0.5151264917719658 1101 38-39 0.58257766713308 1101 40-41 0.5524113071713543 1101 42-43 0.5008884559230964 1101 44-45 0.4785431237545339 1101 46-47 0.4275812468467137 1101 48-49 0.46807110827715803 1101 50-51 0.5025564470450519 1101 52-53 0.4775466918735063 1101 54-55 0.4976956623978026 1101 56-57 0.4979877943711841 1101 58-59 0.49072050461979444 1101 60-61 0.46107748746486266 1101 62-63 0.47476750421185443 1101 64-65 0.46177651213122317 1101 66-67 0.3707117946772627 1101 68-69 0.39019870479022245 1101 70-71 0.3533575504098394 1101 72-73 0.40361646330512 1101 74-75 0.3789544001392855 1101 76-77 0.3346236381655956 1101 78-79 0.3724860697045784 1101 80-81 0.4218916827213377 1101 82-83 0.40711315712844254 1101 84-85 0.39586801095571644 1101 86-87 0.3615914263264415 1101 88-89 0.3773540937205837 1101 90-91 0.3727547195657621 1101 92-93 0.3683484119084568 1101 94-95 0.38872026213169164 1101 96-97 0.4069563507061815 1101 98-99 0.39097231548922906 1101 100 0.6643568669470596 1102 1 0.29813120908929847 1102 2 0.3457960844997743 1102 3 0.2911730314839289 1102 4 0.21332089647620478 1102 5 0.19338264787280224 1102 6 0.2419719030775127 1102 7 0.205328516576607 1102 8 0.2134150441319278 1102 9 0.31219922672187295 1102 10-11 0.2676008800619627 1102 12-13 0.2943748515454985 1102 14-15 0.4267342950656996 1102 16-17 0.4224237326149449 1102 18-19 0.40583900034411613 1102 20-21 0.3808759343174444 1102 22-23 0.43833151530046166 1102 24-25 0.4351425085164493 1102 26-27 0.46032931484881345 1102 28-29 0.4832821002141827 1102 30-31 0.5430878957590579 1102 32-33 0.500745999251933 1102 34-35 0.5155037457333975 1102 36-37 0.5172059771357596 1102 38-39 0.5362869835162485 1102 40-41 0.5153754785684583 1102 42-43 0.49386238957027473 1102 44-45 0.5565979808359813 1102 46-47 0.5844728344062844 1102 48-49 0.6165402766059884 1102 50-51 0.6123805683623473 1102 52-53 0.6226877100241808 1102 54-55 0.5945179094201052 1102 56-57 0.5141820166733311 1102 58-59 0.5129495521639029 1102 60-61 0.5022512107796402 1102 62-63 0.5224628842244528 1102 64-65 0.486592462370794 1102 66-67 0.41917695054264215 1102 68-69 0.4438603350169217 1102 70-71 0.47826644008613073 1102 72-73 0.5207598326123488 1102 74-75 0.5142202355346228 1102 76-77 0.5211985882203436 1102 78-79 0.4743032433333383 1102 80-81 0.4939804678213733 1102 82-83 0.4653078573615588 1102 84-85 0.4484875814755256 1102 86-87 0.4764812883262124 1102 88-89 0.4638726865984424 1102 90-91 0.4049902182421192 1102 92-93 0.4544368847448155 1102 94-95 0.46976432651076294 1102 96-97 0.4569421099141735 1102 98-99 0.448724007229373 1102 100 0.6799922183675662 1103 1 0.3230206062447536 1103 2 0.3571617702087835 1103 3 0.28888475846272144 1103 4 0.25102927423913 1103 5 0.2160267106720255 1103 6 0.2626885565524688 1103 7 0.27407904436436326 1103 8 0.24944326771641556 1103 9 0.3485385767163649 1103 10-11 0.3322385099335321 1103 12-13 0.37184112322415785 1103 14-15 0.4503788287589572 1103 16-17 0.4737554753092894 1103 18-19 0.3860058993566682 1103 20-21 0.37387541118137335 1103 22-23 0.36183315269488503 1103 24-25 0.4031031974530208 1103 26-27 0.4255535584484491 1103 28-29 0.49463266793489424 1103 30-31 0.44436779001544124 1103 32-33 0.4392658820007469 1103 34-35 0.4914704382109747 1103 36-37 0.4507364764695012 1103 38-39 0.45347296948103377 1103 40-41 0.49003594107513493 1103 42-43 0.43807746003977854 1103 44-45 0.4650421913442173 1103 46-47 0.46857779940411604 1103 48-49 0.5048212056841948 1103 50-51 0.49174322617982824 1103 52-53 0.41586602642775716 1103 54-55 0.37163403417697083 1103 56-57 0.2802486810976461 1103 58-59 0.3913267676201002 1103 60-61 0.42984588695770043 1103 62-63 0.38663742059421935 1103 64-65 0.4258332674135161 1103 66-67 0.41603813413263424 1103 68-69 0.38064164911182274 1103 70-71 0.337925439099962 1103 72-73 0.350901183119241 1103 74-75 0.3379895776140813 1103 76-77 0.293941857226784 1103 78-79 0.31401598008640264 1103 80-81 0.31272106604285455 1103 82-83 0.29705010442777535 1103 84-85 0.30683283625209157 1103 86-87 0.2884466124916685 1103 88-89 0.29342676140618096 1103 90-91 0.2872899701997653 1103 92-93 0.2788625622095964 1103 94-95 0.23838870407903556 1103 96-97 0.2833248064909668 1103 98-99 0.2463940658120265 1103 100 0.4966366046380699 1104 1 0.2653553891344984 1104 2 0.29544058780660265 1104 3 0.1955843525530554 1104 4 0.19287291799791006 1104 5 0.19026331110720918 1104 6 0.21709372572995989 1104 7 0.22290770742710464 1104 8 0.2273617583722256 1104 9 0.22394107474865166 1104 10-11 0.2580541657033564 1104 12-13 0.294660532489047 1104 14-15 0.38078703722250395 1104 16-17 0.33135914510333464 1104 18-19 0.3426083517370415 1104 20-21 0.3682072530610725 1104 22-23 0.39804403783931974 1104 24-25 0.40732205828176404 1104 26-27 0.40164453811302536 1104 28-29 0.40668607051806305 1104 30-31 0.3784030354505603 1104 32-33 0.442375634220852 1104 34-35 0.4599961475455956 1104 36-37 0.3986812358097893 1104 38-39 0.44684043430882525 1104 40-41 0.5189454974614733 1104 42-43 0.48705467796357027 1104 44-45 0.42304296362540583 1104 46-47 0.45408474984726865 1104 48-49 0.45089941078253304 1104 50-51 0.35273705668928557 1104 52-53 0.35516901221382824 1104 54-55 0.43688852128222777 1104 56-57 0.4148281714919051 1104 58-59 0.4119746582860344 1104 60-61 0.3685504905839565 1104 62-63 0.39402146717598896 1104 64-65 0.37566739819557426 1104 66-67 0.30834606411247023 1104 68-69 0.3317920717424201 1104 70-71 0.30156073493437674 1104 72-73 0.2755234139389131 1104 74-75 0.33054653915488075 1104 76-77 0.33927634861694855 1104 78-79 0.2958363213966777 1104 80-81 0.22348374211411937 1104 82-83 0.19905555235173722 1104 84-85 0.2609299403859424 1104 86-87 0.2651406822795366 1104 88-89 0.30283661183221966 1104 90-91 0.3223718090576 1104 92-93 0.19842177244894543 1104 94-95 0.2512168097189047 1104 96-97 0.2676600105135165 1104 98-99 0.2490041279256161 1104 100 0.4784009102340363 1105 1 0.18514677609840646 1105 2 0.18284131957557292 1105 3 0.15141704648472398 1105 4 0.19194728437938835 1105 5 0.16934230318027943 1105 6 0.14652313963478036 1105 7 0.22162801667937515 1105 8 0.14671909721643317 1105 9 0.201953272631755 1105 10-11 0.1944989091715712 1105 12-13 0.19403522005003282 1105 14-15 0.2596432102227908 1105 16-17 0.26221205192867103 1105 18-19 0.24953846282855352 1105 20-21 0.2588708577815879 1105 22-23 0.22919733807545128 1105 24-25 0.24718536820782333 1105 26-27 0.29680397289040883 1105 28-29 0.2655977509919438 1105 30-31 0.31304136731068866 1105 32-33 0.30180367552484455 1105 34-35 0.27525106300331714 1105 36-37 0.312035783265614 1105 38-39 0.2971526705549934 1105 40-41 0.3255994724719642 1105 42-43 0.2930338167012323 1105 44-45 0.3415333412932995 1105 46-47 0.39032612096878694 1105 48-49 0.359585732737969 1105 50-51 0.3818962904985952 1105 52-53 0.3693279953805657 1105 54-55 0.3741270762709945 1105 56-57 0.30745570361899155 1105 58-59 0.3696304905280101 1105 60-61 0.35195457858998225 1105 62-63 0.40889139194008806 1105 64-65 0.4009717307911771 1105 66-67 0.3728528337008754 1105 68-69 0.39260535640507044 1105 70-71 0.3674637249732484 1105 72-73 0.28934164085193004 1105 74-75 0.3578203196911005 1105 76-77 0.34919918352075996 1105 78-79 0.40121823947455937 1105 80-81 0.26645911844789794 1105 82-83 0.2316715897982533 1105 84-85 0.29288002600787877 1105 86-87 0.24886128778327077 1105 88-89 0.28992837530479676 1105 90-91 0.27406630035091695 1105 92-93 0.28246171866181413 1105 94-95 0.2858332204154337 1105 96-97 0.31347955566815244 1105 98-99 0.3070740167309083 1105 100 0.4826641865044472 1106 1 0.16566691573723347 1106 2 0.20851418785656506 1106 3 0.16769652534382828 1106 4 0.1382467801227989 1106 5 0.07694751786637966 1106 6 0.16526885588012874 1106 7 0.19352130812216473 1106 8 0.11704257635330606 1106 9 0.12545093395429774 1106 10-11 0.16129134949402868 1106 12-13 0.17645997495555577 1106 14-15 0.24269520862817728 1106 16-17 0.21207156663689375 1106 18-19 0.20946644789334812 1106 20-21 0.2456995517502918 1106 22-23 0.23884615261497544 1106 24-25 0.2035834370328402 1106 26-27 0.2551513630245239 1106 28-29 0.2630765960729846 1106 30-31 0.2472969764517785 1106 32-33 0.26417762270246925 1106 34-35 0.2877122421076592 1106 36-37 0.2589345566145198 1106 38-39 0.376088731342918 1106 40-41 0.3246716054547534 1106 42-43 0.32341679150589897 1106 44-45 0.36255447693154963 1106 46-47 0.3541698137556395 1106 48-49 0.35427958831981243 1106 50-51 0.3808145618266181 1106 52-53 0.391217826536284 1106 54-55 0.33106449297152096 1106 56-57 0.26513340905438554 1106 58-59 0.2594463831676421 1106 60-61 0.31237039126291677 1106 62-63 0.3134499292245536 1106 64-65 0.3148845840225789 1106 66-67 0.32850781720159006 1106 68-69 0.31898060978690523 1106 70-71 0.304426275135107 1106 72-73 0.24890679797869808 1106 74-75 0.27266271395451724 1106 76-77 0.3114678351672211 1106 78-79 0.327063962585342 1106 80-81 0.2661551961366726 1106 82-83 0.24495083039581544 1106 84-85 0.29076308658117966 1106 86-87 0.2169342214178016 1106 88-89 0.3156064133373917 1106 90-91 0.24738224835120803 1106 92-93 0.28992920328756355 1106 94-95 0.20300369660805018 1106 96-97 0.17351854563931113 1106 98-99 0.19899774018729488 1106 100 0.516613640001637 1107 1 0.13645681636621276 1107 2 0.17285810402704982 1107 3 0.14357225435220755 1107 4 0.10152667902652723 1107 5 0.019406471959818816 1107 6 0.01901463390111502 1107 7 0.07872434996281186 1107 8 0.09069859822785986 1107 9 0.128520483888515 1107 10-11 0.1699227101792431 1107 12-13 0.05608568024912586 1107 14-15 0.1342473561829678 1107 16-17 0.1439589357681399 1107 18-19 0.17803250982222352 1107 20-21 0.19367272021028725 1107 22-23 0.16981816675612293 1107 24-25 0.11465593827638543 1107 26-27 0.20820565395437285 1107 28-29 0.245886538392611 1107 30-31 0.12070454482901027 1107 32-33 0.20856234985457434 1107 34-35 0.16691126591673822 1107 36-37 0.1334558692713017 1107 38-39 0.1674703137616973 1107 40-41 0.24878195881149878 1107 42-43 0.2268184563751774 1107 44-45 0.1570766611079506 1107 46-47 0.19583844924640914 1107 48-49 0.2162874613435477 1107 50-51 0.1324420098630057 1107 52-53 0.14742610698062464 1107 54-55 0.19781883159780023 1107 56-57 0.08227534417775217 1107 58-59 0.07386965505390464 1107 60-61 0.08620037616250897 1107 62-63 0.022800912411334195 1107 64-65 0.004264706053113798 1107 66-67 -0.013255640513996525 1107 68-69 -0.02376914357896709 1107 70-71 0.03140483590047438 1107 72-73 0.04699215235814336 1107 74-75 0.09794153724294574 1107 76-77 0.12214425816016217 1107 78-79 0.03797317617819829 1107 80-81 0.012882921547515025 1107 82-83 0.12804304947450618 1107 84-85 0.11718640930607904 1107 86-87 0.10357758654480165 1107 88-89 0.05029575512664053 1107 90-91 0.09109146997746365 1107 92-93 0.02350820980262114 1107 94-95 0.014707323315441556 1107 96-97 0.02177689962923779 1107 98-99 0.06042860200162181 1107 100 0.08505420236301475 1108 1 0.10554091324708992 1108 2 0.12187839444174386 1108 3 0.101419228353258 1108 4 0.1134957709701041 1108 5 0.12235487206417872 1108 6 0.12013345452326973 1108 7 0.1286940427817811 1108 8 0.06201562833336283 1108 9 0.0814760332079345 1108 10-11 0.10025413806929606 1108 12-13 0.15887717418946323 1108 14-15 0.1872025326450455 1108 16-17 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-0.5181781450572771 2215 14-15 -0.63688753638165 2215 16-17 -0.6881492476883224 2215 18-19 -0.6882669011527085 2215 20-21 -0.6427529481388561 2215 22-23 -0.6328521851292095 2215 24-25 -0.6715615797403132 2215 26-27 -0.6732421304135627 2215 28-29 -0.6375381703601875 2215 30-31 -0.702468372108541 2215 32-33 -0.669632216835609 2215 34-35 -0.7049752859376426 2215 36-37 -0.825795318310071 2215 38-39 -0.9184695626145185 2215 40-41 -0.9060039911918238 2215 42-43 -0.9031626304279925 2215 44-45 -0.9485670972796925 2215 46-47 -0.9291076800972959 2215 48-49 -0.9316597835088416 2215 50-51 -0.9602144342000614 2215 52-53 -0.8770551094062853 2215 54-55 -0.9095606283041917 2215 56-57 -0.9328497894508985 2215 58-59 -0.8941624678897426 2215 60-61 -0.8058403281376698 2215 62-63 -0.8496920244653481 2215 64-65 -0.8409156793798473 2215 66-67 -0.8487888577857916 2215 68-69 -0.8187482550021628 2215 70-71 -0.8469545051315919 2215 72-73 -0.8241948347858568 2215 74-75 -0.8394675077615972 2215 76-77 -0.741060072172786 2215 78-79 -0.8477284227470108 2215 80-81 -0.9505985098807628 2215 82-83 -0.93082090185613 2215 84-85 -0.8456929393980488 2215 86-87 -0.7493732273764664 2215 88-89 -0.7885636166466412 2215 90-91 -0.8977136928391474 2215 92-93 -0.7714660661316373 2215 94-95 -0.86277591169506 2215 96-97 -0.7647980768515339 2215 98-99 -0.7974321085585814 2215 100 -0.4295389355523369 2216 1 -0.39479068776736526 2216 2 -0.2507254404459651 2216 3 -0.2799247313491975 2216 4 -0.38030886539664266 2216 5 -0.40435865122056924 2216 6 -0.4045929133723618 2216 7 -0.3890278168032637 2216 8 -0.44323987473521953 2216 9 -0.5267211683764685 2216 10-11 -0.622976684383751 2216 12-13 -0.569431070306976 2216 14-15 -0.7597981756426151 2216 16-17 -0.6491298083203674 2216 18-19 -0.6280719652344047 2216 20-21 -0.6945523272147085 2216 22-23 -0.6890441628098358 2216 24-25 -0.7008810886277104 2216 26-27 -0.7442962733501375 2216 28-29 -0.7169087668578698 2216 30-31 -0.6670801644949478 2216 32-33 -0.630297168055904 2216 34-35 -0.6774961205167358 2216 36-37 -0.8964771275130374 2216 38-39 -0.9681471019905956 2216 40-41 -1.0850191105097196 2216 42-43 -1.017755512325742 2216 44-45 -1.015598653505748 2216 46-47 -1.016109358894326 2216 48-49 -1.0556871024852086 2216 50-51 -1.017470117937826 2216 52-53 -1.0230144159207342 2216 54-55 -0.980739293272265 2216 56-57 -0.8322343805674848 2216 58-59 -0.9289801995698355 2216 60-61 -0.9441839122132194 2216 62-63 -0.9888382862718075 2216 64-65 -0.8229640389360355 2216 66-67 -0.8770674325021872 2216 68-69 -0.8355701062682783 2216 70-71 -0.9079646388531089 2216 72-73 -0.8475680058819108 2216 74-75 -0.7964018346421469 2216 76-77 -0.8645005730385478 2216 78-79 -0.859761033802549 2216 80-81 -0.8959358206320971 2216 82-83 -0.8864030430678369 2216 84-85 -0.7965848221976017 2216 86-87 -0.8703422909743637 2216 88-89 -0.8483476405771597 2216 90-91 -0.8899442289132793 2216 92-93 -0.8678247754547073 2216 94-95 -0.8997002125159952 2216 96-97 -0.9034245681243078 2216 98-99 -0.9526027591086788 2216 100 -0.3542739047322421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 12.0 13 57.0 14 226.0 15 555.0 16 1454.0 17 2951.0 18 5416.0 19 8593.0 20 12233.0 21 17322.0 22 24157.0 23 28262.0 24 29317.0 25 31207.0 26 36872.0 27 45792.0 28 59040.0 29 73965.0 30 92573.0 31 115218.0 32 145722.0 33 188571.0 34 267395.0 35 396783.0 36 668152.0 37 1252818.0 38 859931.0 39 246712.0 40 247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.184701215815224 21.549819627041288 12.724056695831083 25.541422461312408 2 14.836726846965398 20.61685041135722 38.40328730119867 26.143135440478716 3 17.52354302972793 26.797137452087753 29.192554530401456 26.48676498778287 4 12.194199807184022 16.969520577527692 38.737346180207844 32.09893343508044 5 12.894195500930964 38.9689665985305 34.70176004704909 13.435077853489453 6 29.987470606450405 38.74917706734994 17.260898490661287 14.002453835538372 7 26.33196965708306 32.09683763867885 23.785496168970376 17.78569653526772 8 27.1814979586066 33.89025309461709 19.137575256594523 19.790673690181794 9 26.46902694014775 13.948589152988708 21.322933083757412 38.25945082310613 10-11 26.5566437879102 25.18696509377746 26.501456341417086 21.75493477689526 12-13 26.50860444946952 23.02093975795713 27.12846265947904 23.341993133094302 14-15 23.858983072607742 23.90230239773278 24.835972107342577 27.4027424223169 16-17 22.468034650343615 26.431100478883916 26.21340822923976 24.887456641532708 18-19 21.710297842147988 27.09902422253152 28.465147373873034 22.72553056144746 20-21 23.666990057152077 26.31246373241483 27.48583124654672 22.534714963886376 22-23 24.32025112565043 25.579283868611807 27.324443203118427 22.776021802619333 24-25 23.772030525054884 25.51470696658568 28.149912957795593 22.563349550563842 26-27 23.635135299235223 25.91884387829184 26.719734076741126 23.72628674573181 28-29 22.490803537894802 26.430319831310733 27.757366494085726 23.32151013670874 30-31 23.406134502107314 26.17111231003158 27.754753493181045 22.66799969468006 32-33 23.979151505478846 26.231753447209577 26.685949384546127 23.103145662765453 34-35 23.730211668252537 26.168726998002413 27.634815667423318 22.466245666321736 36-37 23.4683911460687 25.365646649417243 27.002892299258647 24.163069905255405 38-39 23.566394943485452 25.60313698890353 27.883603712065945 22.946864355545067 40-41 23.003103507796503 24.680107972233234 28.6282439159364 23.688544604033865 42-43 23.30236258651093 24.853227413914087 27.657703820575964 24.186706178999017 44-45 23.630852579910123 25.690157508658686 27.443079949587514 23.23590996184368 46-47 23.560453348067334 25.751340762206944 26.52211097512423 24.166094914601494 48-49 24.69701116065814 25.625179440518558 26.565979031806187 23.11183036701712 50-51 23.61263746986917 25.337163855323453 26.831258256432317 24.218940418375055 52-53 23.136756877721968 25.837634846025942 26.48732878880794 24.53827948744415 54-55 23.063256740241258 25.929664521042355 26.213278121310896 24.79380061740549 56-57 24.568475369267986 25.262492746482966 25.828017701617416 24.341014182631632 58-59 22.763181048819874 25.97396387703973 27.156428488484742 24.10642658565566 60-61 23.12259679813061 26.54865299261247 26.12587812009656 24.202872089160362 62-63 23.853998520239156 25.749443354911012 25.448731404324263 24.94782672052557 64-65 22.703447190133126 26.037519247854245 26.235912283779456 25.02312127823317 66-67 23.721087925709956 25.59533987134251 26.11861335454386 24.564958848403677 68-69 22.92451831876269 25.294304135090194 26.805507728844667 24.975669817302446 70-71 22.776074318067973 25.259198140313515 26.50797023981195 25.456757301806558 72-73 23.517568706749103 25.124050276073866 27.077769615881902 24.280611401295122 74-75 23.633254241860307 25.606760694588225 26.55339715296719 24.20658791058428 76-77 23.26940218249853 25.638941965168105 25.765027568282633 25.326628284050727 78-79 23.200712471250203 25.790067102101904 25.771429135229777 25.23779129141811 80-81 22.73447400400212 26.520918319109644 26.50989167213843 24.234716004749806 82-83 23.740587775579435 25.07678536268453 27.816294543533683 23.36633231820236 84-85 23.869177778606613 25.649737312091624 26.247279660054 24.23380524924776 86-87 22.136758178801255 26.42263262118036 26.827756184680396 24.61285301533799 88-89 23.461831537988477 24.86733328186842 26.53856962812552 25.132265552017586 90-91 22.86881044055412 25.189220297877768 26.378569402605105 25.563399858963002 92-93 23.348865328752378 25.364421466420445 26.455268026670392 24.831445178156784 94-95 22.109565621668693 25.33345577935083 27.304146366215654 25.25283223276482 96-97 22.533522307871788 25.234573753414246 26.63720228053178 25.594701658182185 98-99 21.868074463369847 25.908446086310132 26.499277033608614 25.72420241671141 100 22.746699723496945 25.015617442844718 25.526767288058988 26.710915545599352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 206.0 1 134.5 2 96.5 3 119.5 4 80.5 5 63.5 6 133.0 7 301.5 8 469.5 9 493.5 10 384.0 11 283.0 12 278.5 13 317.0 14 363.0 15 519.0 16 742.0 17 971.0 18 1321.5 19 1811.5 20 2343.0 21 2879.5 22 3525.0 23 4138.0 24 4731.0 25 5409.5 26 6263.5 27 7105.0 28 8697.0 29 13073.0 30 20790.0 31 28299.5 32 40257.0 33 53845.0 34 63518.5 35 72684.5 36 82545.0 37 102156.0 38 115428.5 39 118577.0 40 113491.5 41 104842.0 42 105633.5 43 114586.0 44 133036.0 45 169893.0 46 206005.0 47 233516.0 48 253749.0 49 248890.5 50 234306.0 51 241929.0 52 248079.0 53 237431.0 54 230358.0 55 197540.0 56 168259.0 57 137268.5 58 104008.0 59 103949.0 60 86092.5 61 54745.0 62 38318.5 63 32297.5 64 23751.5 65 9751.0 66 4434.5 67 3056.0 68 2194.5 69 1551.5 70 1103.5 71 795.5 72 570.5 73 403.5 74 244.5 75 129.5 76 62.0 77 19.0 78 9.5 79 3.5 80 1.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007741421767403453 3 0.0 4 0.08790959060239104 5 0.004380300271752094 6 0.0 7 4.3369309621307865E-5 8 0.0 9 0.0 10-11 0.0 12-13 3.794814591864438E-4 14-15 0.009758094664794268 16-17 0.0 18-19 5.421163702663483E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.252698221598089E-5 60-61 0.0 62-63 0.0 64-65 6.505396443196178E-5 66-67 2.0600422070121238E-4 68-69 0.0 70-71 5.638010250770022E-4 72-73 4.0116611399709773E-4 74-75 3.9032378659177076E-4 76-77 1.4095025626925054E-4 78-79 3.252698221598089E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.06347098463078406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4611556.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.44717711556462 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93831176230155 44.923444960151514 2 16.0648863339945 20.064136044807366 3 5.836193788510254 10.933614815755739 4 2.6049369378078326 6.506838333206491 5 1.3707000780737855 4.279817527389597 6 0.7564525504506237 2.8342995838506404 7 0.44476279025944343 1.9441926516420929 8 0.2726970845364605 1.3623330509557159 9 0.18356675212184484 1.0316902934053613 >10 0.5162678980117085 4.591282197809756 >50 0.005925080153668308 0.24521894503796937 >100 0.004422724863811937 0.4886671695269726 >500 6.700497590150681E-4 0.27664472570540083 >1k 1.0308457774901273E-4 0.15678143724384336 >5k 1.0308457774901273E-4 0.3610382635114332 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6076 0.1317559626295333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5141 0.11148081038157186 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1684654810653933E-5 2 0.0 0.0 0.0 0.0 2.1684654810653933E-5 3 0.0 0.0 0.0 0.0 2.1684654810653933E-5 4 0.0 0.0 0.0 0.0 2.1684654810653933E-5 5 0.0 0.0 0.0 0.0 2.1684654810653933E-5 6 0.0 0.0 0.0 0.0 2.1684654810653933E-5 7 0.0 0.0 0.0 0.0 2.1684654810653933E-5 8 0.0 0.0 0.0 0.0 2.1684654810653933E-5 9 0.0 0.0 0.0 0.0 2.1684654810653933E-5 10-11 0.0 0.0 0.0 0.0 2.1684654810653933E-5 12-13 0.0 0.0 0.0 0.0 1.7347723848523146E-4 14-15 0.0 0.0 0.0 0.0 2.168465481065393E-4 16-17 0.0 0.0 0.0 0.0 2.3853120291719324E-4 18-19 0.0 0.0 0.0 0.0 2.819005125385011E-4 20-21 0.0 0.0 0.0 1.0842327405326966E-5 3.577968043757899E-4 22-23 0.0 0.0 0.0 6.505396443196178E-5 4.120084414024247E-4 24-25 0.0 0.0 0.0 2.3853120291719324E-4 4.120084414024247E-4 26-27 0.0 0.0 0.0 6.50539644319618E-4 4.336930962130786E-4 28-29 0.0 0.0 0.0 0.003664706663000514 4.5537775102373254E-4 30-31 0.0 0.0 0.0 0.01321679710709357 4.662200784290595E-4 32-33 0.0 0.0 0.0 0.030553678628211386 4.770624058343865E-4 34-35 0.0 0.0 0.0 0.0590689997042213 4.770624058343865E-4 36-37 0.0 0.0 0.0 0.10843411638067499 5.095893880503674E-4 38-39 0.0 0.0 0.0 0.18635792344275987 5.421163702663483E-4 40-41 0.0 0.0 0.0 0.2721315755463015 5.421163702663483E-4 42-43 0.0 0.0 0.0 0.36217710464754194 5.421163702663483E-4 44-45 0.0 0.0 0.0 0.4717930347153976 5.529586976716753E-4 46-47 0.0 0.0 0.0 0.6081569864921949 5.638010250770022E-4 48-49 0.0 0.0 0.0 0.7647960905169535 6.50539644319618E-4 50-51 0.0 0.0 0.0 0.9380673247814837 6.939089539409258E-4 52-53 0.0 0.0 0.0 1.1079774375503626 7.589629183728876E-4 54-55 0.0 0.0 0.0 1.2945413652138238 7.589629183728876E-4 56-57 0.0 0.0 0.0 1.521438750825101 7.806475731835415E-4 58-59 0.0 0.0 0.0 1.8011924825373475 8.023322279941955E-4 60-61 0.0 0.0 0.0 2.0863023239878253 8.023322279941955E-4 62-63 0.0 0.0 0.0 2.3861794153643587 9.107555020474651E-4 64-65 0.0 0.0 0.0 2.702948852838391 9.43282484263446E-4 66-67 0.0 0.0 0.0 3.0601276445520775 9.54124811668773E-4 68-69 0.0 0.0 0.0 3.4638200208346164 9.54124811668773E-4 70-71 0.0 0.0 0.0 3.9095806274498237 9.54124811668773E-4 72-73 0.0 0.0 0.0 4.375030900633105 9.649671390741E-4 74-75 0.0 0.0 0.0 4.828153447556529 9.758094664794269E-4 76-77 0.0 0.0 0.0 5.352802828372896 9.758094664794269E-4 78-79 0.0 0.0 0.0 5.91137785164053 9.758094664794269E-4 80-81 0.0 0.0 0.0 6.516813413953988 9.866517938847538E-4 82-83 0.0 0.0 0.0 7.1464598933635415 0.0011276020501540043 84-85 0.0 0.0 0.0 7.758238217209115 0.0011276020501540043 86-87 0.0 0.0 0.0 8.40920071229754 0.0012143406693966201 88 0.0 0.0 0.0 8.930694975838957 0.001301079288639236 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCCG 220 0.0 46.99916 5 GGGCACC 6055 0.0 44.19285 94 CAGTACG 905 0.0 33.760696 9 GTACTAG 6415 0.0 33.586082 1 GTATCAA 16010 0.0 33.43809 1 GGTATCA 10365 0.0 32.99557 1 CTGTGCG 8860 0.0 32.25644 9 TAGTACT 8960 0.0 31.917898 4 TATTCTC 1565 0.0 31.532982 5 ACTGTGC 9325 0.0 31.14763 8 GAACCGT 680 0.0 31.101038 6 TAGGCAT 8090 0.0 30.90674 5 CCGTGTT 680 0.0 30.415178 9 GTACTGT 9590 0.0 30.040915 6 CAACGCA 16635 0.0 29.552824 5 CTAACGC 3110 0.0 29.341347 3 TGAACCG 755 0.0 29.257753 5 TACTGTG 10580 0.0 28.82936 7 TCTAACG 3265 0.0 28.672161 2 ATCAACG 17195 0.0 28.668028 3 >>END_MODULE