FastQCFastQC Report
Mon 16 Jan 2017
ERR1146663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583127
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77900.034.34421
CAACGCA72600.033.724285
GGTATCA50150.032.4402161
GTACTAG33650.032.2779161
ATCAACG78600.031.4522843
GGGCACC42050.031.38619294
TAGTACT46800.029.8263134
CTGTGCG48600.029.7811789
AACGCAG92400.027.615956
CTAGTAC53150.027.5008493
TATCAAC98200.027.0414942
GTACTGT57550.026.7830896
TAGGCAT42200.026.7263955
GTACATG167900.026.6882921
GCATCGT35300.026.0920838
CTAACGC16050.026.0617713
TACATGG171500.025.6510662
TAACGCC16650.025.4048864
ACTGTGC65050.025.2841368
ACATGGG167600.025.0418533
Produced by FastQC (version 0.11.5)