##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146663_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4583127
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33404311074077	31.0	31.0	34.0	28.0	34.0
2	31.437023455819574	31.0	31.0	34.0	28.0	34.0
3	31.581306169346824	33.0	31.0	34.0	28.0	34.0
4	34.98599296942895	37.0	35.0	37.0	32.0	37.0
5	35.18105847819622	37.0	35.0	37.0	32.0	37.0
6	35.23310765771928	37.0	35.0	37.0	32.0	37.0
7	35.153934857140115	37.0	35.0	37.0	32.0	37.0
8	35.155041088758836	37.0	35.0	37.0	32.0	37.0
9	36.67008529329429	39.0	37.0	39.0	32.0	39.0
10-11	36.50930630986224	39.0	37.0	39.0	32.0	39.0
12-13	36.46338929730727	39.0	37.0	39.0	32.0	39.0
14-15	37.6714483364742	40.0	38.0	41.0	31.5	41.0
16-17	37.698060625420155	40.0	38.0	41.0	32.0	41.0
18-19	37.654175849807345	40.0	37.5	41.0	31.0	41.0
20-21	37.71089629416771	40.0	38.0	41.0	31.0	41.0
22-23	37.66427899554169	40.0	37.5	41.0	31.0	41.0
24-25	37.635235942621705	40.0	37.0	41.0	31.0	41.0
26-27	37.679138173565775	40.0	37.0	41.0	31.0	41.0
28-29	37.65994145481895	40.0	37.0	41.0	31.0	41.0
30-31	37.51791211546178	40.0	37.0	41.0	31.0	41.0
32-33	37.50630792033474	40.0	37.0	41.0	31.0	41.0
34-35	37.445993750555026	40.0	37.0	41.0	30.5	41.0
36-37	37.33661799029353	40.0	36.5	41.0	30.0	41.0
38-39	37.24107928931491	40.0	36.0	41.0	30.0	41.0
40-41	37.156275835253965	40.0	36.0	41.0	30.0	41.0
42-43	36.9913500105932	40.0	36.0	41.0	30.0	41.0
44-45	36.8594309081987	39.5	35.0	41.0	30.0	41.0
46-47	36.861215170341126	39.0	35.0	41.0	30.0	41.0
48-49	36.73502272575035	39.0	35.0	41.0	30.0	41.0
50-51	36.570975013347876	39.0	35.0	41.0	29.0	41.0
52-53	36.37217755475683	39.0	35.0	41.0	29.0	41.0
54-55	36.15639584065639	38.5	35.0	40.5	29.0	41.0
56-57	35.925193650535974	38.0	34.5	40.0	28.0	41.0
58-59	35.650803152520105	37.5	34.0	40.0	28.0	41.0
60-61	35.38462996988737	37.0	34.0	40.0	28.0	41.0
62-63	35.0726415611001	36.5	34.0	40.0	27.5	41.0
64-65	34.91431821548912	36.0	34.0	39.5	28.0	41.0
66-67	34.63714893783218	35.5	33.0	39.0	27.0	41.0
68-69	34.49856571724938	35.0	33.0	39.0	27.0	41.0
70-71	34.3629526303766	35.0	33.5	38.5	27.5	41.0
72-73	34.11503597871061	35.0	33.0	37.5	27.0	40.0
74-75	33.84180309644485	35.0	33.0	37.0	27.0	39.5
76-77	33.56730644819574	35.0	33.0	36.5	26.5	39.0
78-79	33.309667613400194	35.0	33.0	36.0	26.0	39.0
80-81	33.09728336133823	35.0	33.0	36.0	26.0	38.0
82-83	32.855377998471354	35.0	33.0	35.5	26.0	37.0
84-85	32.671630744685885	35.0	33.0	35.0	26.0	37.0
86-87	32.49758112747039	35.0	33.0	35.0	25.5	36.5
88-89	32.358109212334725	35.0	32.0	35.0	25.0	36.0
90-91	32.20626124914278	35.0	32.0	35.0	25.0	36.0
92-93	32.06916293177126	35.0	32.0	35.0	25.0	36.0
94-95	31.981732450355402	35.0	32.0	35.0	25.0	35.0
96-97	31.87724363736811	35.0	32.0	35.0	25.0	35.0
98-99	31.774991725082025	35.0	32.0	35.0	24.0	35.0
100	30.824750656047716	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30661841716741733
1101	2	0.4395987712811191
1101	3	0.4691920068728166
1101	4	0.5297714548241075
1101	5	0.41201103732119293
1101	6	0.45223562491921854
1101	7	0.4074639723885696
1101	8	0.47886441815171565
1101	9	0.5866907931003524
1101	10-11	0.6170353463512299
1101	12-13	0.5105210496735637
1101	14-15	0.6685889462830445
1101	16-17	0.6846017200699066
1101	18-19	0.699535150380548
1101	20-21	0.6963901276816529
1101	22-23	0.7158356290596259
1101	24-25	0.7636951889067021
1101	26-27	0.7355199239522534
1101	28-29	0.7311389733550442
1101	30-31	0.7325603859493697
1101	32-33	0.7403554406943798
1101	34-35	0.7318883746632139
1101	36-37	0.745411518620358
1101	38-39	0.6999193002910147
1101	40-41	0.7458737910779334
1101	42-43	0.7300016647610121
1101	44-45	0.7115511759748614
1101	46-47	0.6504404658373844
1101	48-49	0.6364987330792147
1101	50-51	0.6264115803414185
1101	52-53	0.610199430039728
1101	54-55	0.6259407096925145
1101	56-57	0.6382671841294041
1101	58-59	0.6377540057327735
1101	60-61	0.6721987990732998
1101	62-63	0.7129057286653122
1101	64-65	0.6723852652282645
1101	66-67	0.7228330537651999
1101	68-69	0.701763220507722
1101	70-71	0.6336586657490528
1101	72-73	0.5455454116242748
1101	74-75	0.544614384874869
1101	76-77	0.580762658854205
1101	78-79	0.5400247349657263
1101	80-81	0.4683371601070334
1101	82-83	0.4952214311119434
1101	84-85	0.4815353143146126
1101	86-87	0.49493838478623076
1101	88-89	0.5527896205405014
1101	90-91	0.5246321379845753
1101	92-93	0.48482085687940213
1101	94-95	0.4901464728826497
1101	96-97	0.4997869963843584
1101	98-99	0.5359906501601408
1101	100	0.5716191325290083
1102	1	0.3370899470786668
1102	2	0.39854148173078485
1102	3	0.3995634243771562
1102	4	0.4556536212394988
1102	5	0.3549467755042244
1102	6	0.3996089203025548
1102	7	0.3753117027942565
1102	8	0.40002426002253344
1102	9	0.5348330410107636
1102	10-11	0.6650199967315586
1102	12-13	0.5491116293286709
1102	14-15	0.7110497557647193
1102	16-17	0.7041019934921309
1102	18-19	0.7423918524528617
1102	20-21	0.6619610247343957
1102	22-23	0.7273531366262134
1102	24-25	0.6877358851460116
1102	26-27	0.6566404613788279
1102	28-29	0.7136999759411609
1102	30-31	0.6907794294273089
1102	32-33	0.6084162363150583
1102	34-35	0.7288753662213026
1102	36-37	0.6967343647557982
1102	38-39	0.6881058701332989
1102	40-41	0.5712479665390475
1102	42-43	0.6630951998445056
1102	44-45	0.6759053678792881
1102	46-47	0.6108447984403185
1102	48-49	0.5818023029480912
1102	50-51	0.5353882549575886
1102	52-53	0.5479038558720575
1102	54-55	0.526848011015197
1102	56-57	0.5404414604149608
1102	58-59	0.473279550773384
1102	60-61	0.5149213771030645
1102	62-63	0.5588216741175813
1102	64-65	0.5363902979060171
1102	66-67	0.5606158036712046
1102	68-69	0.5320200949879421
1102	70-71	0.5031409008997656
1102	72-73	0.5472957647946401
1102	74-75	0.5142367014446307
1102	76-77	0.5175385409256776
1102	78-79	0.4813764992985554
1102	80-81	0.5077206642026724
1102	82-83	0.5599114196813701
1102	84-85	0.5280835723872102
1102	86-87	0.47056530965968335
1102	88-89	0.5932262986124215
1102	90-91	0.5148250620453467
1102	92-93	0.5120723891830963
1102	94-95	0.5659724833106665
1102	96-97	0.5608832628419123
1102	98-99	0.44132091192394896
1102	100	0.4530147441023651
1103	1	0.31390633818131164
1103	2	0.3818806976973761
1103	3	0.3886290412314395
1103	4	0.4873440085599725
1103	5	0.34885584270994485
1103	6	0.4098793776072327
1103	7	0.3564870603262662
1103	8	0.386458895386923
1103	9	0.526293346384243
1103	10-11	0.6467346985246394
1103	12-13	0.5937557000787805
1103	14-15	0.7048408153720302
1103	16-17	0.7087294746609132
1103	18-19	0.7586770399205918
1103	20-21	0.654466199661556
1103	22-23	0.7094483654711397
1103	24-25	0.6671682579496476
1103	26-27	0.6133651445129118
1103	28-29	0.6151337937244676
1103	30-31	0.6374130045843955
1103	32-33	0.5877044213331928
1103	34-35	0.644284843791624
1103	36-37	0.5755267882327999
1103	38-39	0.619972685526264
1103	40-41	0.6238552122518826
1103	42-43	0.6822463654947626
1103	44-45	0.6370626738134959
1103	46-47	0.6134703633397791
1103	48-49	0.6397179461723681
1103	50-51	0.5477870387895152
1103	52-53	0.48973529954062656
1103	54-55	0.47686082102008953
1103	56-57	0.4811482208383353
1103	58-59	0.5605024204144797
1103	60-61	0.5529708184442654
1103	62-63	0.5080740550099918
1103	64-65	0.505749918955793
1103	66-67	0.5145990207592632
1103	68-69	0.41112758347514244
1103	70-71	0.45636206852777406
1103	72-73	0.46298128014664996
1103	74-75	0.4080940286378052
1103	76-77	0.356731568023811
1103	78-79	0.4206094610198363
1103	80-81	0.3557652796858619
1103	82-83	0.38118708320907047
1103	84-85	0.43475268685893553
1103	86-87	0.45780405407735003
1103	88-89	0.44184268845582153
1103	90-91	0.4559755662234366
1103	92-93	0.31827537132631534
1103	94-95	0.3437101947799093
1103	96-97	0.3855983918512109
1103	98-99	0.3268901613196924
1103	100	0.49568386579620594
1104	1	0.2583965396341483
1104	2	0.3172442551753214
1104	3	0.30661403323097147
1104	4	0.4213472048093081
1104	5	0.34858447740105447
1104	6	0.26901407081857087
1104	7	0.3288739734758508
1104	8	0.3573322925754425
1104	9	0.42616209774498515
1104	10-11	0.5278947917078654
1104	12-13	0.41255817056349997
1104	14-15	0.5786699512455584
1104	16-17	0.579367685598875
1104	18-19	0.5246959376843776
1104	20-21	0.5322527500183298
1104	22-23	0.5436831551211867
1104	24-25	0.5604753827185434
1104	26-27	0.5316629784316191
1104	28-29	0.46161284114587886
1104	30-31	0.4491860844835571
1104	32-33	0.5643550360595171
1104	34-35	0.5660367352727036
1104	36-37	0.5474859374853338
1104	38-39	0.5339982719862988
1104	40-41	0.51135180844053
1104	42-43	0.49594806302906136
1104	44-45	0.4351139308500933
1104	46-47	0.43879085852128696
1104	48-49	0.3759682485220921
1104	50-51	0.35645241543068806
1104	52-53	0.40137233436836084
1104	54-55	0.4356123544689865
1104	56-57	0.40524615825616905
1104	58-59	0.4070000361134163
1104	60-61	0.35602799361596027
1104	62-63	0.36602465140069995
1104	64-65	0.4120190660937695
1104	66-67	0.38769001167261763
1104	68-69	0.3535483392120895
1104	70-71	0.33835522860073297
1104	72-73	0.28681646972608377
1104	74-75	0.25879896993833285
1104	76-77	0.24366693243140958
1104	78-79	0.3241035231531413
1104	80-81	0.3389880579422382
1104	82-83	0.25394241335618517
1104	84-85	0.33113659758516434
1104	86-87	0.30298414981449895
1104	88-89	0.2677952657014231
1104	90-91	0.33434865043580686
1104	92-93	0.2712729982557036
1104	94-95	0.328298837633902
1104	96-97	0.33297256000356157
1104	98-99	0.3344360632203056
1104	100	0.4987902715119077
1105	1	0.16435863670285045
1105	2	0.26464226762925946
1105	3	0.25858444163516836
1105	4	0.32561889655159604
1105	5	0.19756692809547616
1105	6	0.18798303707262676
1105	7	0.26921545435696714
1105	8	0.3046008322291627
1105	9	0.3486369920979513
1105	10-11	0.35647108847166464
1105	12-13	0.2886432971479067
1105	14-15	0.4512042433903076
1105	16-17	0.34478953266232537
1105	18-19	0.31907310290579005
1105	20-21	0.31070004251242267
1105	22-23	0.38604375178358197
1105	24-25	0.3484463829229796
1105	26-27	0.41299327119984497
1105	28-29	0.45837618611592745
1105	30-31	0.5085560943860514
1105	32-33	0.44573736211162185
1105	34-35	0.4786861156707829
1105	36-37	0.42985145173849304
1105	38-39	0.4858035068844089
1105	40-41	0.5159947149346422
1105	42-43	0.3961884455822542
1105	44-45	0.41587738747558234
1105	46-47	0.42776613739923164
1105	48-49	0.3205020877100111
1105	50-51	0.35600018449292037
1105	52-53	0.4198017514332406
1105	54-55	0.41213214539614995
1105	56-57	0.4374587533860108
1105	58-59	0.4191948957133036
1105	60-61	0.4368286225290987
1105	62-63	0.399351854243136
1105	64-65	0.3582703995910279
1105	66-67	0.30159515285141936
1105	68-69	0.37068583285888224
1105	70-71	0.3533345122927898
1105	72-73	0.31895027254019226
1105	74-75	0.292216513383984
1105	76-77	0.28740412455571374
1105	78-79	0.3339063364743424
1105	80-81	0.2925832444048453
1105	82-83	0.360939012616484
1105	84-85	0.33549109384820497
1105	86-87	0.28937528490029507
1105	88-89	0.2674658095571374
1105	90-91	0.3017403449866478
1105	92-93	0.3558692523079898
1105	94-95	0.33439392478124574
1105	96-97	0.24582901769373677
1105	98-99	0.2878855102949025
1105	100	0.36598120403414214
1106	1	0.10153790938417728
1106	2	0.1877238381662636
1106	3	0.17672292315981153
1106	4	0.2853360794690616
1106	5	0.16820587352495409
1106	6	0.2121960727733665
1106	7	0.16293298417263458
1106	8	0.15647877201053007
1106	9	0.2989520898914435
1106	10-11	0.30201235101959867
1106	12-13	0.24167231185104043
1106	14-15	0.3095873024435889
1106	16-17	0.25672883868423924
1106	18-19	0.34652005582908174
1106	20-21	0.30377979570327795
1106	22-23	0.38511418616506177
1106	24-25	0.4245582572549935
1106	26-27	0.4020438657635026
1106	28-29	0.4569197751697587
1106	30-31	0.445410886030956
1106	32-33	0.40239154288259016
1106	34-35	0.32756978734762043
1106	36-37	0.3968401852858534
1106	38-39	0.39580737710410574
1106	40-41	0.36610909081986875
1106	42-43	0.3493544972700846
1106	44-45	0.3326644254612745
1106	46-47	0.35751522544558867
1106	48-49	0.25410770542517014
1106	50-51	0.28266674475167974
1106	52-53	0.19769620639510777
1106	54-55	0.2932709354616705
1106	56-57	0.2867160408978364
1106	58-59	0.2998995583312478
1106	60-61	0.29827593496812455
1106	62-63	0.24235170476736556
1106	64-65	0.21114886813138156
1106	66-67	0.24918856343437312
1106	68-69	0.27938652162507793
1106	70-71	0.24930270608540184
1106	72-73	0.25890623438610305
1106	74-75	0.20863708547327775
1106	76-77	0.19265007865781314
1106	78-79	0.2880849561559913
1106	80-81	0.23186679658518727
1106	82-83	0.24433964369529804
1106	84-85	0.17773979075086999
1106	86-87	0.19726631672391193
1106	88-89	0.18633668662916847
1106	90-91	0.19083191711009562
1106	92-93	0.23222310369391863
1106	94-95	0.2505352707395119
1106	96-97	0.26211213011078627
1106	98-99	0.2500032850926921
1106	100	0.23836239182231722
1107	1	0.11209141786860499
1107	2	0.16245071606365258
1107	3	0.22876998803399218
1107	4	0.23518734427705112
1107	5	0.13255019553467662
1107	6	0.21521785692665674
1107	7	0.1284531882607638
1107	8	0.2172181251053189
1107	9	0.2023372166617179
1107	10-11	0.28438652315390556
1107	12-13	0.20550914346739546
1107	14-15	0.32444996623563327
1107	16-17	0.25633605378442326
1107	18-19	0.19672412948633422
1107	20-21	0.23969808213105637
1107	22-23	0.3246523093982461
1107	24-25	0.3196518614407111
1107	26-27	0.315460014077658
1107	28-29	0.32098333206448615
1107	30-31	0.45499078490034606
1107	32-33	0.35800443688973616
1107	34-35	0.32579742062068817
1107	36-37	0.30657632968241444
1107	38-39	0.29477523672935035
1107	40-41	0.2768617236989215
1107	42-43	0.3294308807558437
1107	44-45	0.32457794950029495
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2209	7	-0.006216879068126957
2209	8	0.07044630156192966
2209	9	0.03790049548369723
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2210	4	0.2861508601525813
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2210	9	0.18716246314216534
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2210	24-25	0.12390957008206982
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2210	28-29	0.1401922028665581
2210	30-31	0.18270108780936312
2210	32-33	0.12846883279092225
2210	34-35	0.15589627777191595
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2211	2	-0.18862581890652308
2211	3	-0.14459517736262129
2211	4	0.010446838181863427
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2211	16-17	-0.11297260161631328
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2211	22-23	-0.13198520525547508
2211	24-25	-0.03133683306677426
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2211	28-29	-0.11554405801875589
2211	30-31	-0.07027040499209392
2211	32-33	-0.08636083330721789
2211	34-35	-0.15078822254382374
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2211	50-51	0.00937333005369112
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2211	78-79	0.0014406812195062457
2211	80-81	0.09971335641206736
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2211	90-91	0.00937210884197981
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2212	100	-0.16147088812722643
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2213	34-35	-0.5721465031416813
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2213	84-85	-0.5383341888319464
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2213	88-89	-0.47113925640734067
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2213	98-99	-0.5377151547289465
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2214	2	-0.7572125201551678
2214	3	-0.6432477040501787
2214	4	-0.5813759812790522
2214	5	-0.5675649540905638
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2214	9	-1.1659044711467104
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2214	28-29	-0.7942555260657471
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2214	78-79	-0.7702556978837123
2214	80-81	-0.7852779587733423
2214	82-83	-0.7012975637647116
2214	84-85	-0.7339954314324117
2214	86-87	-0.7414516828244899
2214	88-89	-0.7685447673448493
2214	90-91	-0.7672661177601654
2214	92-93	-0.6449811765149747
2214	94-95	-0.6804557501654571
2214	96-97	-0.6894816773128838
2214	98-99	-0.7080239217058875
2214	100	-0.5343516471320768
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2215	2	-0.9957978036446313
2215	3	-0.8261861398820294
2215	4	-2.349214461626353
2215	5	-1.0262141444870423
2215	6	-0.6820479151914967
2215	7	-0.6797795977316525
2215	8	-0.6101303744939273
2215	9	-0.9114876951057624
2215	10-11	-0.7219597396721866
2215	12-13	-0.8911150777155186
2215	14-15	-0.876938388128977
2215	16-17	-0.8809909546309953
2215	18-19	-0.7725852202951913
2215	20-21	-0.8674552092202248
2215	22-23	-0.8087894189683382
2215	24-25	-0.9047288342414035
2215	26-27	-0.8873960569017072
2215	28-29	-0.7924675075016552
2215	30-31	-3.608091910197075
2215	32-33	-1.414102222161148
2215	34-35	-1.2502353486123425
2215	36-37	-0.9932780903232583
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2215	40-41	-0.7747611674536259
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2215	64-65	-0.8962771020714086
2215	66-67	-0.7693650599978099
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2215	80-81	-0.7213316831529539
2215	82-83	-0.6860387397391321
2215	84-85	-0.7170417933568523
2215	86-87	-0.7243380626638398
2215	88-89	-0.7098745774364836
2215	90-91	-0.7395982218402324
2215	92-93	-0.747023398773635
2215	94-95	-0.7735422812188233
2215	96-97	-1.039388415806357
2215	98-99	-0.9117712517209746
2215	100	-0.4595312543351113
2216	1	-0.5076000513553431
2216	2	-0.6769891288145615
2216	3	-0.6865797742399451
2216	4	-0.5447020074625613
2216	5	-0.5258290510072072
2216	6	-0.4489375506266384
2216	7	-0.5784086282900631
2216	8	-0.6418179517202063
2216	9	-0.8175776154340326
2216	10-11	-1.1550229789358823
2216	12-13	-0.7806463481597206
2216	14-15	-0.8999638980541036
2216	16-17	-0.9522929226580956
2216	18-19	-1.1373840912813833
2216	20-21	-1.2248480338441325
2216	22-23	-1.0878100335466385
2216	24-25	-1.162972494490596
2216	26-27	-1.4670972811419176
2216	28-29	-1.3937023530817356
2216	30-31	-1.400520045921354
2216	32-33	-1.7091913201517883
2216	34-35	-1.3673860516890741
2216	36-37	-1.418554763377955
2216	38-39	-1.3503599716336865
2216	40-41	-1.2360850546778366
2216	42-43	-1.225984683210271
2216	44-45	-1.1543969810126455
2216	46-47	-1.2474049161967784
2216	48-49	-1.276499620728238
2216	50-51	-1.3276858219466234
2216	52-53	-1.3184783336058956
2216	54-55	-1.3256886090479796
2216	56-57	-1.1667253650129439
2216	58-59	-1.1719143907678813
2216	60-61	-1.237735022961381
2216	62-63	-1.3485784051172018
2216	64-65	-1.2064926115041246
2216	66-67	-1.199640995403442
2216	68-69	-1.2557006294038544
2216	70-71	-1.1901403567849727
2216	72-73	-1.1411037827131878
2216	74-75	-1.1428442586419791
2216	76-77	-1.1360492035261487
2216	78-79	-0.935552186906655
2216	80-81	-1.0689090328947586
2216	82-83	-1.115427221517514
2216	84-85	-1.094169394843803
2216	86-87	-1.0540192923303593
2216	88-89	-1.1405185014163024
2216	90-91	-1.138799012807084
2216	92-93	-1.0996092706649314
2216	94-95	-1.0888134477478282
2216	96-97	-1.1054511624841332
2216	98-99	-1.165694870311352
2216	100	-0.8356237920033216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	9.0
3	23.0
4	36.0
5	30.0
6	52.0
7	187.0
8	510.0
9	729.0
10	1128.0
11	2003.0
12	3548.0
13	5300.0
14	7590.0
15	10033.0
16	12582.0
17	15013.0
18	17394.0
19	20186.0
20	23238.0
21	26337.0
22	29823.0
23	34464.0
24	39630.0
25	45346.0
26	51383.0
27	56420.0
28	64937.0
29	78434.0
30	93137.0
31	108820.0
32	135065.0
33	176023.0
34	249097.0
35	361004.0
36	590193.0
37	1038431.0
38	961243.0
39	323109.0
40	639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39482178744177	19.169189220462652	11.783741181068239	24.652247811027337
2	16.211189760623412	19.560576671927667	39.190107138048766	25.038126429400155
3	19.109583257050947	25.048807998161806	28.71926547208632	27.122343272700927
4	12.471190451355778	15.7652440208594	38.21988957558041	33.54367595220441
5	13.586728242524218	37.986194526905614	34.64364227088795	13.783434959682214
6	32.02389080211101	36.927928948145315	17.291767719051567	13.756412530692105
7	28.292590114887457	31.508343490227343	22.302030026004267	17.897036368880933
8	26.967169815684645	34.711609185897046	19.493836990016952	18.827384008401356
9	26.45934424777274	14.99298619297886	20.123374927423626	38.424294631824765
10-11	25.89974686394616	25.49735621576491	27.459834302417235	21.143062617871692
12-13	25.91337947514759	23.330399376668762	27.450553847959807	23.305667300223842
14-15	23.217928739405146	24.7670365309935	24.578562386076566	27.436472343524787
16-17	22.854947214813368	27.529082909748503	25.65509096018768	23.96087891525045
18-19	22.53182924035304	27.743205943640152	27.054603375481395	22.670361440525415
20-21	23.778582935939376	26.90698641320149	26.373863389284324	22.94056726157481
22-23	23.988008908441046	26.61108746126231	26.144260538080488	23.256643092216155
24-25	23.560805460622767	26.698169588181887	26.7564056995021	22.984619251693246
26-27	23.433740322448493	26.906983460159907	26.006452810184328	23.652823407207276
28-29	22.85301231949763	27.35736034860603	26.323514923885625	23.466112408010712
30-31	23.36370565281685	27.09910163889641	26.339506351980983	23.197686356305756
32-33	23.637363010227546	27.17440459519958	25.670423301383554	23.51780909318932
34-35	23.700985748201685	26.995861086015154	26.353373159802317	22.949780005980845
36-37	23.450681059779097	26.670834472115253	26.19817042069195	23.6803140474137
38-39	23.542452140060753	26.844498006742786	26.566241721400914	23.046808131795547
40-41	23.291010223138976	26.2699278765848	26.98506318899704	23.453998711279187
42-43	23.26332362966616	26.433149443748555	26.468152477053806	23.835374449531475
44-45	23.31797388894454	27.077807872511812	26.46568883878192	23.13852939976173
46-47	23.36752492079522	26.905194862587134	26.145647324168657	23.58163289244899
48-49	23.787214665783964	26.706271086587442	26.163829042205855	23.34268520542274
50-51	23.430242905168114	26.68557872570127	26.2717765016248	23.612401867505817
52-53	23.187134273190672	26.959396521846923	26.16904904700422	23.68442015795819
54-55	23.07644380264405	26.91848772496734	26.246317719346735	23.758750753041873
56-57	23.772257726830475	26.560224492465835	25.989598739794022	23.677919040909668
58-59	23.073802381178147	26.77537308408649	26.58053691897637	23.57028761575899
60-61	22.976717937817753	27.19819159041971	26.23225017452763	23.5928402972349
62-63	23.276498661237422	26.80452538848119	25.900255095917373	24.018720854364016
64-65	22.728354391128242	26.910948653124166	26.218446722173166	24.142250233574426
66-67	23.1470989666877	26.653080913427868	26.145933772126178	24.053886347758258
68-69	22.895072501843	26.630242728186303	26.370286305554135	24.104398464416562
70-71	22.802115253562683	26.35234848876637	26.4834062268355	24.362130030835445
72-73	23.18233558894008	26.462084760203446	26.7486280280405	23.606951622815973
74-75	23.258719794107744	26.677211647297348	26.447778123773208	23.6162904348217
76-77	23.138678645192893	26.541091443915032	26.247909231459126	24.07232067943295
78-79	23.023948835596514	26.48730698838488	26.286513152798186	24.20223102322042
80-81	22.684263075897633	26.756043105725425	26.70824314240734	23.851450675969602
82-83	23.176550092257525	26.095394455471126	27.3907965917958	23.337258860475547
84-85	23.260370848261992	26.451781281007037	26.55076376464252	23.73708410608845
86-87	22.42931196392685	26.95136272081334	26.829065514708233	23.790259800551574
88-89	23.137940466421682	26.097588092193284	26.584152739140972	24.18031870224406
90-91	22.892180963273272	26.088205596875607	26.75066846393066	24.26894497592046
92-93	23.102770166457876	26.187847787373485	26.75839310313676	23.95098894303188
94-95	22.35274235013258	26.155350616673235	27.19481894760064	24.297088085593547
96-97	22.611005575004967	26.001636812817107	26.904480592333773	24.482877019844146
98-99	22.130021512448295	26.511907675297213	26.773369515823163	24.584701296431323
100	22.687167854955863	25.99442511546522	26.252144838484337	25.06626219109458
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	687.5
2	646.0
3	831.0
4	807.0
5	719.5
6	686.0
7	673.0
8	687.5
9	671.5
10	604.5
11	565.0
12	599.0
13	645.0
14	698.5
15	814.5
16	917.0
17	1097.5
18	1368.0
19	1671.5
20	2202.0
21	2993.5
22	4016.0
23	5499.0
24	7226.0
25	9317.0
26	12403.5
27	15856.5
28	20477.5
29	28203.5
30	38419.0
31	47874.0
32	60604.5
33	71519.0
34	77866.5
35	87583.0
36	97012.5
37	112135.5
38	120676.5
39	116749.0
40	113045.5
41	110179.0
42	113015.0
43	122179.0
44	134671.0
45	157037.0
46	177969.5
47	192283.5
48	204284.0
49	205830.0
50	199503.5
51	203603.0
52	208221.0
53	203420.5
54	196260.5
55	176964.0
56	160185.0
57	139766.5
58	118645.5
59	111257.0
60	95310.0
61	73626.5
62	58448.0
63	46609.0
64	34126.0
65	21893.5
66	14890.5
67	10669.5
68	7811.5
69	5662.5
70	4057.5
71	2750.0
72	1670.0
73	1004.5
74	614.5
75	358.0
76	192.5
77	112.0
78	74.5
79	63.0
80	48.5
81	50.5
82	47.5
83	38.0
84	32.5
85	27.0
86	21.5
87	15.5
88	14.5
89	14.0
90	11.0
91	9.0
92	8.5
93	9.0
94	10.5
95	7.5
96	4.0
97	4.0
98	8.0
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060700914462985645
2	0.007593069098892524
3	0.008160367364901736
4	0.00970952801438843
5	0.0035347045805189338
6	0.002356469720345956
7	7.200324145501532E-4
8	0.0012655115164820875
9	5.236599378546569E-4
10-11	4.0365453542963133E-4
12-13	2.181916407727737E-4
14-15	1.0909582038638685E-4
16-17	5.018407737773794E-4
18-19	0.0016800756339503575
20-21	8.400378169751788E-4
22-23	0.0012655115164820875
24-25	0.0013091498446366422
26-27	0.0035456141625575728
28-29	0.004112912428566784
30-31	0.004931131081464686
32-33	0.005618434749898923
34-35	0.0035237949984802956
36-37	0.008280372767326762
38-39	0.006578477969299127
40-41	0.006283919254255883
42-43	0.014553382439544005
44-45	0.02029182259186795
46-47	0.0187535715244199
48-49	0.021350052049615906
50-51	0.018884486508883563
52-53	0.02520113450925536
54-55	0.019168135641888167
56-57	0.015306143600210076
58-59	0.017793528305019693
60-61	0.016538926370576246
62-63	0.011716891109497947
64-65	0.011793258183768418
66-67	0.013756982950723383
68-69	0.00531296645281704
70-71	0.009033133927992832
72-73	0.008694936884795033
74-75	0.01169507194542067
76-77	0.010516837085247692
78-79	0.01234964686773899
80-81	0.010636842487672719
82-83	0.006611206715415043
84-85	0.01086594371048413
86-87	0.009567703447886126
88-89	0.008411287751790425
90-91	0.013222413430830086
92-93	0.008858580615374612
94-95	0.007669436173162995
96-97	0.009611341776040682
98-99	0.009414969299345185
100	0.26772114322819335
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4583127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7690136204707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4922877845444	48.247920021371804
2	15.064848087806926	19.514707019789775
3	5.141151115559423	9.989618598856994
4	2.2016797250701896	5.704024964039413
5	1.100806880502756	3.5649087918395432
6	0.6075575118873627	2.3610540457591136
7	0.38157131345187933	1.7299798318701969
8	0.2382206591404282	1.2343453693235125
9	0.1652878565742402	0.9634978290379835
>10	0.5933577551764758	5.91039516850839
>50	0.010277574919657312	0.44726281213656444
>100	0.002905289817771284	0.2852189916166365
>500	0.0	0.0
>1k	4.844554849013879E-5	0.047066555850134605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0909582038638685E-4
2	0.0	0.0	0.0	0.0	1.0909582038638685E-4
3	0.0	0.0	0.0	0.0	1.0909582038638685E-4
4	0.0	0.0	0.0	0.0	1.0909582038638685E-4
5	0.0	0.0	0.0	0.0	1.3091498446366422E-4
6	0.0	0.0	0.0	0.0	1.5273414854094158E-4
7	0.0	0.0	0.0	0.0	1.5273414854094158E-4
8	0.0	0.0	0.0	0.0	1.7455331261821897E-4
9	0.0	0.0	0.0	0.0	1.9637247669549633E-4
10-11	0.0	0.0	0.0	0.0	1.9637247669549633E-4
12-13	0.0	0.0	0.0	0.0	5.56388683970573E-4
14-15	0.0	0.0	0.0	0.0	7.309419965887919E-4
16-17	0.0	0.0	0.0	0.0	8.182186528979014E-4
18-19	0.0	0.0	0.0	0.0	8.509473990138174E-4
20-21	0.0	0.0	0.0	2.181916407727737E-5	9.491336373615656E-4
22-23	0.0	0.0	0.0	6.545749223183211E-5	9.927719655161203E-4
24-25	1.0909582038638686E-5	0.0	0.0	2.181916407727737E-4	0.0010255007116320364
26-27	2.181916407727737E-5	0.0	0.0	5.127503558160182E-4	0.001112777367941146
28-29	2.181916407727737E-5	0.0	0.0	0.003381970431977992	0.0011345965320184233
30-31	2.181916407727737E-5	0.0	0.0	0.011313236574068316	0.0011564156960957007
32-33	2.181916407727737E-5	0.0	0.0	0.026248454384964676	0.0012327827703661714
34-35	2.181916407727737E-5	0.0	0.0	0.04872219338456037	0.0013636977548298357
36-37	2.181916407727737E-5	0.0	0.0	0.08834579534889607	0.0013964265009457518
38-39	2.181916407727737E-5	0.0	0.0	0.14891579482741804	0.0014073360829843905
40-41	2.181916407727737E-5	0.0	0.0	0.2167515759436734	0.001418245665023029
42-43	2.181916407727737E-5	0.0	0.0	0.28510010741574476	0.0015164319033707772
44-45	2.181916407727737E-5	0.0	0.0	0.3688856974724899	0.0016146181417185253
46-47	2.181916407727737E-5	0.0	0.0	0.4741958928914691	0.0017346235441435508
48-49	2.181916407727737E-5	0.0	0.0	0.5977359998970135	0.0018109906184140218
50-51	2.181916407727737E-5	0.0	0.0	0.7252144660185066	0.0018109906184140218
52-53	2.181916407727737E-5	0.0	0.0	0.8537293424336703	0.0020946397514186275
54-55	2.181916407727737E-5	0.0	0.0	0.994986610669964	0.0021164589154959047
56-57	2.181916407727737E-5	0.0	0.0	1.170838163550781	0.002138278079573182
58-59	2.181916407727737E-5	0.0	0.0	1.3774220090344431	0.0021928259897663754
60-61	2.181916407727737E-5	0.0	0.0	1.594249952052387	0.0022255547358822915
62-63	2.181916407727737E-5	0.0	0.0	1.8216165513196558	0.002356469720345956
64-65	2.181916407727737E-5	0.0	0.0	2.0619982819590206	0.002421927212577788
66-67	2.181916407727737E-5	0.0	0.0	2.3340614388385923	0.0024437463766550658
68-69	2.181916407727737E-5	0.0	0.0	2.645366362311147	0.002465565540732343
70-71	2.181916407727737E-5	0.0	0.0	2.9880145149807107	0.002498294286848259
72-73	2.181916407727737E-5	0.0	0.0	3.3403285573365085	0.0026510284353892005
74-75	2.181916407727737E-5	0.0	0.0	3.6963518575854435	0.002771033837814226
76-77	2.181916407727737E-5	0.0	0.0	4.09243514308026	0.0028474009120846968
78-79	2.181916407727737E-5	0.0	0.0	4.537644276495065	0.0029237679863551675
80-81	2.181916407727737E-5	0.0	0.0	5.021320596178112	0.0029892254785869998
82-83	2.181916407727737E-5	0.0	0.0	5.528277963931613	0.0032728746115916053
84-85	2.181916407727737E-5	0.0	0.0	6.021118332527115	0.0032728746115916053
86-87	2.181916407727737E-5	0.0	0.0	6.538363436143053	0.0033928800140166308
88	2.181916407727737E-5	0.0	0.0	6.962211607926204	0.0035128854164416566
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7790	0.0	34.3442	1
CAACGCA	7260	0.0	33.72428	5
GGTATCA	5015	0.0	32.440216	1
GTACTAG	3365	0.0	32.277916	1
ATCAACG	7860	0.0	31.452284	3
GGGCACC	4205	0.0	31.386192	94
TAGTACT	4680	0.0	29.826313	4
CTGTGCG	4860	0.0	29.781178	9
AACGCAG	9240	0.0	27.61595	6
CTAGTAC	5315	0.0	27.500849	3
TATCAAC	9820	0.0	27.041494	2
GTACTGT	5755	0.0	26.783089	6
TAGGCAT	4220	0.0	26.726395	5
GTACATG	16790	0.0	26.688292	1
GCATCGT	3530	0.0	26.092083	8
CTAACGC	1605	0.0	26.061771	3
TACATGG	17150	0.0	25.651066	2
TAACGCC	1665	0.0	25.404886	4
ACTGTGC	6505	0.0	25.284136	8
ACATGGG	16760	0.0	25.041853	3
>>END_MODULE