FastQCFastQC Report
Mon 16 Jan 2017
ERR1146663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4583127
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132060.2881438808045249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116520.2542368998284359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75410.16453831630674864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83950.049.384821
GTATCAA147300.040.157881
GTACTAG33400.032.6873551
CAACGCA180500.031.8975015
ATCAACG180650.031.8804743
GTGGTAT26500.030.3660641
GGGCACC39800.030.36547394
TATCAAC196800.030.1050192
AACGCAG200700.029.223476
TCAACGC204500.028.3694634
CTGTGCG47850.027.800319
CTAACGC16000.027.6432553
TAGTACT48400.027.5120164
TAGGCAT44800.026.6473145
ACGCAGA218350.026.3016267
GTACATG165750.026.1483541
TAACGCC17300.026.1099764
CGCAGAG221650.025.868488
CTAGTAC53300.025.8655533
TACATGG166700.025.3500612
Produced by FastQC (version 0.11.5)