FastQCFastQC Report
Mon 16 Jan 2017
ERR1146662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481258
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63150.044.10568294
GTACTAG69550.037.5170441
TAGGCAT85300.033.5507975
CAACGCA106650.033.3997275
GTATCAA122950.033.382471
GCATCGT73200.033.25328
CTGTGCG89550.032.9540529
TAGTACT94250.031.4147364
ATCAACG114300.031.2493933
ACTGTGC96300.031.0833598
TATTCTC32200.030.9395925
CTAGGCA96100.030.5654534
GTACTGT100450.029.7526686
AGGGCTA77200.029.218545
TACTGTG111950.028.87887
CTAACGC33100.028.8232023
TCTAACG33400.028.7050232
CGCCTAC32950.028.5226157
GGTATCA78550.028.4900681
TAACGCC33600.028.3942874
Produced by FastQC (version 0.11.5)