##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146662_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5481258
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.342112157464584	31.0	31.0	34.0	28.0	34.0
2	31.36140772793399	31.0	31.0	34.0	28.0	34.0
3	31.519386790404685	33.0	31.0	34.0	28.0	34.0
4	34.94883072462562	37.0	35.0	37.0	32.0	37.0
5	35.125588140532706	37.0	35.0	37.0	32.0	37.0
6	35.17948142561434	37.0	35.0	37.0	32.0	37.0
7	35.10043989901588	37.0	35.0	37.0	32.0	37.0
8	35.098774952757196	37.0	35.0	37.0	32.0	37.0
9	36.565573085594586	39.0	37.0	39.0	32.0	39.0
10-11	36.38711104275697	39.0	37.0	39.0	31.0	39.0
12-13	36.29742497069103	39.0	37.0	39.0	31.0	39.0
14-15	37.446404365567176	40.0	37.0	41.0	31.0	41.0
16-17	37.4851150046212	40.0	37.0	41.0	31.0	41.0
18-19	37.461689360362165	40.0	37.0	41.0	31.0	41.0
20-21	37.5418124817332	40.0	37.0	41.0	31.0	41.0
22-23	37.48557028331817	40.0	37.0	41.0	31.0	41.0
24-25	37.45301179035907	40.0	37.0	41.0	31.0	41.0
26-27	37.49341200505431	40.0	37.0	41.0	31.0	41.0
28-29	37.49649979986346	40.0	37.0	41.0	31.0	41.0
30-31	37.36810327118337	40.0	36.0	41.0	30.0	41.0
32-33	37.36424621866002	40.0	36.0	41.0	30.0	41.0
34-35	37.2982440344899	40.0	36.0	41.0	30.0	41.0
36-37	37.1815158126109	40.0	36.0	41.0	30.0	41.0
38-39	37.07837635083041	40.0	36.0	41.0	30.0	41.0
40-41	36.9899708242159	40.0	35.5	41.0	30.0	41.0
42-43	36.82032929301996	39.5	35.0	41.0	30.0	41.0
44-45	36.647294106571884	39.0	35.0	41.0	30.0	41.0
46-47	36.63730543243905	39.0	35.0	41.0	30.0	41.0
48-49	36.492356772843024	39.0	35.0	41.0	29.0	41.0
50-51	36.29740025008857	39.0	35.0	41.0	29.0	41.0
52-53	36.07311888986068	38.0	35.0	40.0	28.5	41.0
54-55	35.864238921065194	38.0	34.0	40.0	28.0	41.0
56-57	35.60919245180577	37.5	34.0	40.0	28.0	41.0
58-59	35.297489919284956	37.0	34.0	40.0	28.0	41.0
60-61	35.043368511389176	36.5	34.0	40.0	27.5	41.0
62-63	34.726850934584725	36.0	33.0	39.5	27.0	41.0
64-65	34.57144007817183	35.5	33.0	39.0	27.0	41.0
66-67	34.31984062782668	35.0	33.0	39.0	26.5	41.0
68-69	34.19791250475711	35.0	33.0	39.0	26.0	41.0
70-71	34.137734348574725	35.0	33.0	38.0	27.0	41.0
72-73	33.929901858296034	35.0	33.0	37.5	26.5	40.0
74-75	33.684371635124634	35.0	33.0	37.0	26.0	40.0
76-77	33.43224055499668	35.0	33.0	36.5	26.0	39.0
78-79	33.19018781819794	35.0	33.0	36.0	26.0	39.0
80-81	33.0143595685516	35.0	33.0	36.0	26.0	39.0
82-83	32.74980570153786	35.0	33.0	35.5	25.5	37.0
84-85	32.57203437605017	35.0	32.5	35.0	25.0	37.0
86-87	32.4007994150248	35.0	32.0	35.0	25.0	36.5
88-89	32.27508657684057	35.0	32.0	35.0	25.0	36.0
90-91	32.1194733033913	35.0	32.0	35.0	25.0	36.0
92-93	31.962105870586644	35.0	32.0	35.0	25.0	36.0
94-95	31.89241466101395	35.0	32.0	35.0	25.0	36.0
96-97	31.783225493125848	35.0	32.0	35.0	24.0	35.0
98-99	31.68341656240228	35.0	32.0	35.0	24.0	35.0
100	30.721102710363205	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3639407915154109
1101	2	0.4661516805501691
1101	3	0.5018025296487814
1101	4	0.6044164657564579
1101	5	0.48280201357542296
1101	6	0.5058632125042308
1101	7	0.4898739718126208
1101	8	0.4968955773564261
1101	9	0.7574226699638018
1101	10-11	0.728256415907012
1101	12-13	0.6405569455884148
1101	14-15	0.8217867290564413
1101	16-17	0.8647195993059711
1101	18-19	0.8377207427447715
1101	20-21	0.7900068781514733
1101	22-23	0.8287488916891874
1101	24-25	0.8709698263420051
1101	26-27	0.8164824830741608
1101	28-29	0.8001539213897573
1101	30-31	0.7944830820621007
1101	32-33	0.7523497365261846
1101	34-35	0.7417299622591784
1101	36-37	0.8272975981482844
1101	38-39	0.813539746323471
1101	40-41	0.7827234265686229
1101	42-43	0.7544703700710684
1101	44-45	0.7661572277300905
1101	46-47	0.7554664739264965
1101	48-49	0.729875911968584
1101	50-51	0.7408759866358565
1101	52-53	0.7879691411363865
1101	54-55	0.7569873141808756
1101	56-57	0.7423126847992592
1101	58-59	0.7159712903285751
1101	60-61	0.6917520050236874
1101	62-63	0.7233769546109556
1101	64-65	0.7123268470266595
1101	66-67	0.7352939579473343
1101	68-69	0.758798716808883
1101	70-71	0.7613390198592072
1101	72-73	0.7721960669579531
1101	74-75	0.6707186200821056
1101	76-77	0.6941239457075241
1101	78-79	0.6873253551055853
1101	80-81	0.59333363701991
1101	82-83	0.6055950199655129
1101	84-85	0.6053865665677591
1101	86-87	0.6101915389577073
1101	88-89	0.6262747639896631
1101	90-91	0.5847473694933214
1101	92-93	0.5659856603744196
1101	94-95	0.5702769452255652
1101	96-97	0.6051604814549556
1101	98-99	0.6330539216648958
1101	100	0.6160862708057522
1102	1	0.3980453837592748
1102	2	0.3918535611977596
1102	3	0.4102343327731397
1102	4	0.5571173709384638
1102	5	0.39548346797261047
1102	6	0.45092559106598884
1102	7	0.4344964063175425
1102	8	0.47124589645969905
1102	9	0.6490302419151988
1102	10-11	0.7248930840984116
1102	12-13	0.6139914668263629
1102	14-15	0.7631811215767783
1102	16-17	0.6724685765187033
1102	18-19	0.687313343140346
1102	20-21	0.6599965729455377
1102	22-23	0.7048732765218233
1102	24-25	0.7101489459522767
1102	26-27	0.6791429099309951
1102	28-29	0.6381535990947853
1102	30-31	0.6915624768201027
1102	32-33	0.6835984751589663
1102	34-35	0.7770142113377361
1102	36-37	0.7110471591755427
1102	38-39	0.7144597154734811
1102	40-41	0.6087979419816492
1102	42-43	0.6631427288522076
1102	44-45	0.721170823423293
1102	46-47	0.6113211431275047
1102	48-49	0.5937068363588338
1102	50-51	0.598515016687017
1102	52-53	0.5578704056870478
1102	54-55	0.5170630043982882
1102	56-57	0.5361147906218235
1102	58-59	0.5392028429377689
1102	60-61	0.5316487906098857
1102	62-63	0.5758201643389711
1102	64-65	0.5898715931481036
1102	66-67	0.6552337535623138
1102	68-69	0.5530311361955
1102	70-71	0.5194224297804411
1102	72-73	0.5953256190087686
1102	74-75	0.516249450612662
1102	76-77	0.5549187931805974
1102	78-79	0.5369591392909214
1102	80-81	0.5350504567855126
1102	82-83	0.6179063856846199
1102	84-85	0.6067611726826669
1102	86-87	0.5948484279765509
1102	88-89	0.6307395513782268
1102	90-91	0.6160690386493357
1102	92-93	0.5812468667010258
1102	94-95	0.6037730473890051
1102	96-97	0.6527063400843112
1102	98-99	0.5187947379401763
1102	100	0.6839294469683956
1103	1	0.2933679012120862
1103	2	0.3954253568887296
1103	3	0.37516334263010265
1103	4	0.4475124123139196
1103	5	0.3127870126553418
1103	6	0.3844569453829223
1103	7	0.3198430996175432
1103	8	0.3467322976843832
1103	9	0.4748025452235254
1103	10-11	0.5670544079573716
1103	12-13	0.44363844547918063
1103	14-15	0.621233864197805
1103	16-17	0.5689163616089061
1103	18-19	0.5986621043085165
1103	20-21	0.5151850191458891
1103	22-23	0.5993309439714807
1103	24-25	0.5842149282952533
1103	26-27	0.5550623352899109
1103	28-29	0.5782938450325403
1103	30-31	0.573839351092424
1103	32-33	0.6044378112787996
1103	34-35	0.5760706747092925
1103	36-37	0.5642865826063002
1103	38-39	0.6035024541231877
1103	40-41	0.5152784004698105
1103	42-43	0.5495885611720439
1103	44-45	0.47179485024487633
1103	46-47	0.4934097208620116
1103	48-49	0.5254991538504328
1103	50-51	0.5273825132963736
1103	52-53	0.48793274974266154
1103	54-55	0.5077064823632114
1103	56-57	0.5562729507065001
1103	58-59	0.5539127962232797
1103	60-61	0.5293454351565714
1103	62-63	0.47376638422927897
1103	64-65	0.44770215807840685
1103	66-67	0.5215684396165585
1103	68-69	0.4997686771192775
1103	70-71	0.4771695922278525
1103	72-73	0.44076614468599473
1103	74-75	0.3875618675091417
1103	76-77	0.3273588887207879
1103	78-79	0.3760720600989771
1103	80-81	0.2988585304527476
1103	82-83	0.3411824996777142
1103	84-85	0.3569373217053169
1103	86-87	0.3114687893286572
1103	88-89	0.39320232694446133
1103	90-91	0.3399092782612243
1103	92-93	0.3724563891176693
1103	94-95	0.2983129335319319
1103	96-97	0.34604607907060725
1103	98-99	0.317088959813141
1103	100	0.39125769505673347
1104	1	0.23118397895817466
1104	2	0.3542807681053688
1104	3	0.27053057883251697
1104	4	0.37736886079926535
1104	5	0.309841053038717
1104	6	0.2517300140681513
1104	7	0.29698461101816065
1104	8	0.3677168052088504
1104	9	0.4167373803931014
1104	10-11	0.5116395245861582
1104	12-13	0.37214996337600326
1104	14-15	0.5012290987170189
1104	16-17	0.4708189540294967
1104	18-19	0.44454830350068164
1104	20-21	0.4118125120772831
1104	22-23	0.4617942334649001
1104	24-25	0.45348306461875154
1104	26-27	0.43594456449473995
1104	28-29	0.4370278720819911
1104	30-31	0.3992506137231402
1104	32-33	0.41780573922710573
1104	34-35	0.4745469001340368
1104	36-37	0.42257495190012406
1104	38-39	0.4691027426111063
1104	40-41	0.4565075644419281
1104	42-43	0.5129689112951468
1104	44-45	0.42733368163230523
1104	46-47	0.4435021475400234
1104	48-49	0.4038748824407108
1104	50-51	0.337289658832745
1104	52-53	0.43616289260857144
1104	54-55	0.4595886379946421
1104	56-57	0.4133444625035594
1104	58-59	0.3807611033977949
1104	60-61	0.3933826192113159
1104	62-63	0.4224903425613604
1104	64-65	0.37465672255167703
1104	66-67	0.35943813857024054
1104	68-69	0.3820785612283686
1104	70-71	0.32673378484068394
1104	72-73	0.37001817889171207
1104	74-75	0.249970991514175
1104	76-77	0.27107906835955475
1104	78-79	0.30969038808969174
1104	80-81	0.3201376259815447
1104	82-83	0.29551079168174965
1104	84-85	0.2934284974414396
1104	86-87	0.3370479498544583
1104	88-89	0.20537570972587105
1104	90-91	0.2975074247018128
1104	92-93	0.29804310591609706
1104	94-95	0.35138713891419115
1104	96-97	0.3055396436106044
1104	98-99	0.23853686673755448
1104	100	0.33333792640726756
1105	1	0.15366953589802534
1105	2	0.2184850560751741
1105	3	0.23915443070046294
1105	4	0.27659265701891655
1105	5	0.19462881540574983
1105	6	0.1961481257614821
1105	7	0.25404164538924334
1105	8	0.3069846240506564
1105	9	0.3194718709337394
1105	10-11	0.3263135986504011
1105	12-13	0.2530682577685539
1105	14-15	0.41113446048758107
1105	16-17	0.35833160793131213
1105	18-19	0.4251838843160485
1105	20-21	0.3279746930257659
1105	22-23	0.41490522092000504
1105	24-25	0.38921870101754763
1105	26-27	0.3598605447176979
1105	28-29	0.40874351184909585
1105	30-31	0.4230217425387437
1105	32-33	0.3658209841655946
1105	34-35	0.35573082724731364
1105	36-37	0.36311075524996994
1105	38-39	0.37868686352052805
1105	40-41	0.38116634032221697
1105	42-43	0.35769844401721684
1105	44-45	0.2788208233655425
1105	46-47	0.33740556041590253
1105	48-49	0.2564965707643836
1105	50-51	0.28330163634129235
1105	52-53	0.347517803094604
1105	54-55	0.3507882073488986
1105	56-57	0.35761218822759133
1105	58-59	0.3773201470279588
1105	60-61	0.3507602999105188
1105	62-63	0.34588297158113335
1105	64-65	0.3679418813119142
1105	66-67	0.30378871285670783
1105	68-69	0.31691957985584196
1105	70-71	0.3488555246339331
1105	72-73	0.2735897410400483
1105	74-75	0.24140234226879898
1105	76-77	0.237026162703728
1105	78-79	0.24495285341645
1105	80-81	0.21122099887578116
1105	82-83	0.25648986773295945
1105	84-85	0.16854232735119723
1105	86-87	0.15094420486162363
1105	88-89	0.17093426515949517
1105	90-91	0.2250663867033751
1105	92-93	0.261102761853504
1105	94-95	0.3021949208912389
1105	96-97	0.2170909970719741
1105	98-99	0.24471468251466177
1105	100	0.3974734616520621
1106	1	0.09339603679199726
1106	2	0.14884948316535684
1106	3	0.12202387419888439
1106	4	0.26742255957277905
1106	5	0.1957258847599732
1106	6	0.16740070696776144
1106	7	0.19111430210682556
1106	8	0.16419263302120868
1106	9	0.2704932782304681
1106	10-11	0.2894871822397036
1106	12-13	0.2106049073071219
1106	14-15	0.2974922476222588
1106	16-17	0.23437598860699183
1106	18-19	0.256370250331166
1106	20-21	0.25005005914945855
1106	22-23	0.25935667449713407
1106	24-25	0.25679955150670963
1106	26-27	0.3014797567576366
1106	28-29	0.22129348153163875
1106	30-31	0.3191463120006901
1106	32-33	0.2669693735936818
1106	34-35	0.2574108538052329
1106	36-37	0.24937406331365253
1106	38-39	0.29570046639548764
1106	40-41	0.26274647791776573
1106	42-43	0.24478738274449086
1106	44-45	0.2883451002419548
1106	46-47	0.30860843027213747
1106	48-49	0.23610419737607913
1106	50-51	0.245498195272134
1106	52-53	0.16235714139349255
1106	54-55	0.23722950092817285
1106	56-57	0.1717316820915613
1106	58-59	0.16237299747097467
1106	60-61	0.17812680921267088
1106	62-63	0.24083721676984737
1106	64-65	0.1603254720792151
1106	66-67	0.2159028298958532
1106	68-69	0.2245455135661345
1106	70-71	0.23162337469587158
1106	72-73	0.14466478574606612
1106	74-75	0.18333739645291303
1106	76-77	0.2215750841287516
1106	78-79	0.21690147529768922
1106	80-81	0.16945451426649072
1106	82-83	0.16677564488082908
1106	84-85	0.1413254412582745
1106	86-87	0.11144445225123434
1106	88-89	0.171617757193971
1106	90-91	0.12951409471946818
1106	92-93	0.18877131276891745
1106	94-95	0.20529782798006835
1106	96-97	0.18110705683394812
1106	98-99	0.19123992976970783
1106	100	0.15678994255771528
1107	1	0.1364016674534092
1107	2	0.1676009385804349
1107	3	0.13954349743279337
1107	4	0.23605753679996155
1107	5	0.13896368288867222
1107	6	0.21467538449276446
1107	7	0.1577506419312371
1107	8	0.20316334271706893
1107	9	0.20871458713347124
1107	10-11	0.3056186310044211
1107	12-13	0.19573368450932094
1107	14-15	0.3608283479022205
1107	16-17	0.19988153882049886
1107	18-19	0.23694236123924384
1107	20-21	0.21537249262768654
1107	22-23	0.3606518823278151
1107	24-25	0.3389496662617617
1107	26-27	0.18505768517388077
1107	28-29	0.2809465417180874
1107	30-31	0.3319449614446981
1107	32-33	0.32711280961545697
1107	34-35	0.22385813931986576
1107	36-37	0.2961819284545939
1107	38-39	0.2934810868581792
1107	40-41	0.2362334085066209
1107	42-43	0.30528385532005586
1107	44-45	0.3418526185745634
1107	46-47	0.25566630328486184
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2209	6	0.0573100671022857
2209	7	0.0745649391435208
2209	8	0.05801176641021044
2209	9	0.07687578727320243
2209	10-11	0.19118370123419481
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2209	14-15	0.18651475840002263
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2209	34-35	0.04882743545821455
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2210	4	0.04464207703003353
2210	5	0.06273707142540275
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2210	7	0.0014044086524336308
2210	8	-0.07143812930124938
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2210	12-13	-0.11381017194449328
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2210	18-19	-0.02321105260084977
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2210	22-23	-0.09071310080869921
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2210	30-31	0.02977407592452863
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2210	36-37	0.052975588953700026
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2211	2	-0.21624663061162508
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2211	48-49	-0.20927850819263938
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2212	16-17	-0.3563682681316749
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2212	22-23	-0.32592220306075603
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2212	34-35	-0.3637849877099555
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2212	98-99	-0.16817849621711645
2212	100	-0.22052390255636212
2213	1	-0.3023254364120156
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2213	32-33	-0.5046978539670164
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2213	70-71	-0.5466085135001535
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2213	100	-0.3759721879398654
2214	1	-0.6150990825829261
2214	2	-0.7306101540656584
2214	3	-0.6982485613232328
2214	4	-0.643992049118161
2214	5	-0.5721852175731854
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2214	8	-0.7493814079164025
2214	9	-1.1946839817143413
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2214	12-13	-0.7765936114863763
2214	14-15	-3.456125070928465
2214	16-17	-1.6299631467725604
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2214	22-23	-0.781581331951088
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2214	80-81	-0.7071951906127651
2214	82-83	-0.6714228526545511
2214	84-85	-0.6821351452992133
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2214	88-89	-0.6805703485812558
2214	90-91	-0.7198855447016612
2214	92-93	-0.7475179526496767
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2214	96-97	-0.6432706571977747
2214	98-99	-0.6585037060747325
2214	100	-0.5176066820617393
2215	1	-0.8585505635644566
2215	2	-1.2193257931632075
2215	3	-0.9488839978591095
2215	4	-2.3478881595398633
2215	5	-1.1266361476737643
2215	6	-0.8609341995218287
2215	7	-0.8602495766108476
2215	8	-0.7726623416434109
2215	9	-1.0707245556419593
2215	10-11	-0.8629063178675267
2215	12-13	-1.0212021261738755
2215	14-15	-1.0174935254620863
2215	16-17	-1.1353944110281375
2215	18-19	-1.0453421589664558
2215	20-21	-1.1027192302933315
2215	22-23	-1.0521972926083833
2215	24-25	-1.091390045262692
2215	26-27	-1.0069438028009117
2215	28-29	-0.9263456342055818
2215	30-31	-3.65159159578905
2215	32-33	-1.5505312392948056
2215	34-35	-1.3541716498544716
2215	36-37	-1.1788362698952852
2215	38-39	-0.9822992702557656
2215	40-41	-1.0141173043182903
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2215	54-55	-1.025833338790001
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2215	64-65	-1.0903977704488312
2215	66-67	-0.9625684783947861
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2215	70-71	-1.130085721990639
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2215	82-83	-0.9399062027152993
2215	84-85	-0.979903226469979
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2215	90-91	-0.9922400410270313
2215	92-93	-1.0062796667042058
2215	94-95	-0.9303972609069575
2215	96-97	-1.1885757801961567
2215	98-99	-1.0143834471844535
2215	100	-0.5492469209223394
2216	1	-0.5377919595010638
2216	2	-0.6607307534628006
2216	3	-0.6812411368132203
2216	4	-0.5269410006758974
2216	5	-0.48298095899198046
2216	6	-0.43618088884585404
2216	7	-0.5526873073499772
2216	8	-0.6133106392244514
2216	9	-0.783514468267974
2216	10-11	-1.0925667383678217
2216	12-13	-0.6524501684748003
2216	14-15	-0.7549041495246058
2216	16-17	-0.8061798216196863
2216	18-19	-1.0914958526780367
2216	20-21	-1.1358316659057124
2216	22-23	-0.9694721687586565
2216	24-25	-1.0646147825750703
2216	26-27	-1.3928107883560727
2216	28-29	-1.3121526862705721
2216	30-31	-1.4923740222122674
2216	32-33	-1.6942813306861666
2216	34-35	-1.3066687792038394
2216	36-37	-1.3808309391817062
2216	38-39	-1.2849083684481855
2216	40-41	-1.1486954793310105
2216	42-43	-1.1092209941945157
2216	44-45	-1.0535015722186714
2216	46-47	-1.1624057936515655
2216	48-49	-1.1643901654982045
2216	50-51	-1.2649607027907876
2216	52-53	-1.2401119747909988
2216	54-55	-1.3109038101846977
2216	56-57	-1.1131867371711053
2216	58-59	-1.0831720084107488
2216	60-61	-1.1829627046137716
2216	62-63	-1.2396961292923834
2216	64-65	-1.1676316445881199
2216	66-67	-1.1832786065581757
2216	68-69	-1.1822956092902217
2216	70-71	-1.176106922284319
2216	72-73	-1.0831003956947285
2216	74-75	-1.1313188244262307
2216	76-77	-1.099181204472778
2216	78-79	-0.9843286104910902
2216	80-81	-1.0930801988277707
2216	82-83	-1.0611018931606537
2216	84-85	-1.0550568649619407
2216	86-87	-1.0821469206963386
2216	88-89	-1.155374643517309
2216	90-91	-1.2229593506547936
2216	92-93	-1.139377181474977
2216	94-95	-1.113337601783254
2216	96-97	-1.1639340246970917
2216	98-99	-1.1015821539294066
2216	100	-0.7791758877112969
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	27.0
4	41.0
5	34.0
6	51.0
7	208.0
8	584.0
9	1085.0
10	1566.0
11	2544.0
12	4575.0
13	6968.0
14	9671.0
15	13063.0
16	16258.0
17	19049.0
18	22152.0
19	25139.0
20	28765.0
21	32843.0
22	37995.0
23	43802.0
24	51247.0
25	58571.0
26	65738.0
27	73024.0
28	84043.0
29	100679.0
30	118208.0
31	137059.0
32	168416.0
33	218939.0
34	318804.0
35	456953.0
36	718649.0
37	1223350.0
38	1003434.0
39	417116.0
40	598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15185275106014	21.688496130936624	12.340514086165383	23.819137031837855
2	15.368826175303479	19.82708556192175	41.139248012310475	23.664840250464298
3	18.03180672209112	27.56174493974844	29.176949431603177	25.229498906557264
4	11.764771352007504	16.860833741883656	41.21179522332756	30.162599682781277
5	12.65178380348797	39.145192297342	35.51372916487641	12.689294734293622
6	28.905679007300318	39.518865986491114	18.367622466445813	13.207832539762757
7	26.220513842183923	32.17495898720284	24.542601495726498	17.06192567488674
8	26.14205335347498	35.12661507193707	20.01052693033252	18.720804644255434
9	26.285350654999206	14.822481385558126	21.93477447024645	36.957393489196214
10-11	25.55535172876689	26.04147405871324	27.818665269414268	20.584508943105597
12-13	25.610223878820264	24.14152510747327	27.739511441421214	22.508739572285247
14-15	23.08377931572744	25.23463847626769	25.203979419663558	26.477602788341315
16-17	21.710758927642935	27.706379621815085	26.61853152045689	23.96432993008509
18-19	21.03455872392383	28.70945157313369	28.305441668327653	21.950548034614826
20-21	23.046421639623187	27.783336673535896	27.014728240845603	22.155513445995307
22-23	23.754943718508255	27.3667271279016	26.612430348255984	22.26589880533416
24-25	23.01448290702805	27.37557413446416	27.67482509030209	21.9351178682057
26-27	22.884847301712988	27.687413224060265	26.417305380153877	23.010434094072874
28-29	21.774065467643158	28.33527046446539	27.075574204072637	22.815089863818816
30-31	22.758282026363712	27.884016372618003	27.058716957668434	22.298984643349854
32-33	23.38803058423962	28.12912595541539	25.897277422480048	22.58556603786494
34-35	23.178885275236606	27.819460666957852	27.16371231435637	21.83794174344918
36-37	22.722166482296142	27.37462576250121	26.862155983849835	23.041051771352816
38-39	22.988934612157685	27.480544094836638	27.536438000959514	21.99408329204616
40-41	22.48648770939854	26.427657611765582	28.251132653254846	22.834722025581033
42-43	22.45493614543634	26.78986829853694	27.396701505938964	23.358494050087756
44-45	22.690220626776572	27.9869237931401	27.17906748543502	22.143788094648308
46-47	22.606078914155987	27.561782294910024	26.691259730471383	23.14087906046261
48-49	23.44879699589067	27.051126043743594	27.029730423395147	22.470346536970585
50-51	22.97042649486513	26.844500743301847	26.8765155483929	23.308557213440125
52-53	22.169171892648265	27.729681400876636	26.72297718676661	23.378169519708482
54-55	22.29010961299017	27.559189746499037	26.69630712037831	23.454393520132484
56-57	23.67256409084411	26.791171188974033	26.414121411889386	23.122143308292475
58-59	22.342480559798254	27.359129643891066	27.508983717499568	22.78940607881111
60-61	22.402790593625184	28.138218100795726	26.528019522623396	22.930971782955694
62-63	22.92052056172468	27.192937561403458	26.099026640330408	23.787515236541463
64-65	21.83606703943233	27.481395928041447	26.708249287721998	23.974287744804226
66-67	22.77812060830746	26.849013177221572	26.66482438338858	23.70804183108239
68-69	21.996887232938736	27.066395388923013	27.013257037912318	23.923460340225933
70-71	22.09510823876872	26.61942153634617	27.157055209239054	24.12841501564605
72-73	22.79489643568262	26.753554273219756	27.662044407045176	22.789504884052448
74-75	22.958515459132176	27.136555485085328	27.04974999288405	22.855179062898447
76-77	22.542263699649595	27.04519157618897	26.76672240489932	23.645822319262113
78-79	22.34108918930261	26.95649555978462	26.832913974590326	23.86950127632244
80-81	21.867187937614617	27.380363518794198	27.568778991469532	23.183669552121653
82-83	22.857389948338	26.154905353841958	28.778564234125525	22.209140463694517
84-85	23.156450716498945	26.866603000577665	27.101117781413176	22.875828501510213
86-87	21.542610597323986	27.69055246160159	27.653659774618887	23.113177166455536
88-89	22.978707837395483	26.142085138910343	27.34092958178973	23.538277441904444
90-91	22.46874427236436	26.22514268854681	27.61578639245602	23.69032664663281
92-93	22.766030021421198	26.48687373609609	27.532043074474803	23.215053168007913
94-95	21.330885297012482	26.609422561861006	28.292198179645496	23.767493961481012
96-97	21.842370449732325	26.4660268149481	27.67121793025004	24.020384805069526
98-99	21.04958215135604	27.350815421787427	27.52226998026275	24.077332446593786
100	22.005868690161183	26.552567067217026	26.46676097836867	24.97480326425312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1128.0
1	742.0
2	401.0
3	455.0
4	454.0
5	401.0
6	332.5
7	332.5
8	408.5
9	428.0
10	358.5
11	323.5
12	324.5
13	336.5
14	382.5
15	649.0
16	819.0
17	915.5
18	1274.0
19	1560.0
20	1908.5
21	2462.0
22	3393.0
23	4657.5
24	6022.5
25	7990.5
26	11080.5
27	14392.5
28	19753.5
29	32334.5
30	50292.5
31	66381.5
32	88016.0
33	108751.5
34	122269.5
35	139615.5
36	155184.0
37	184189.0
38	193699.5
39	172729.5
40	155529.5
41	139080.5
42	134637.5
43	143217.0
44	159704.0
45	196350.0
46	227845.0
47	248397.0
48	261863.0
49	254290.0
50	240426.0
51	248371.5
52	254957.5
53	242345.5
54	228220.5
55	194275.0
56	168022.0
57	135991.5
58	101189.0
59	98214.0
60	82027.5
61	53623.5
62	38386.0
63	32564.5
64	23465.5
65	9445.0
66	4152.5
67	2528.5
68	1728.5
69	1199.0
70	780.5
71	574.5
72	370.5
73	206.5
74	138.0
75	97.5
76	64.5
77	52.5
78	39.0
79	29.5
80	30.0
81	29.5
82	23.5
83	15.0
84	15.5
85	18.5
86	17.0
87	14.0
88	12.5
89	10.0
90	11.5
91	10.0
92	5.0
93	7.5
94	7.5
95	6.5
96	5.0
97	3.5
98	4.5
99	5.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05816183073301785
2	0.007242862861043942
3	0.0073705707704326264
4	0.009067261566596574
5	0.0034481135534944717
6	0.0022622543948852616
7	7.662474563321048E-4
8	0.0014230309903310518
9	5.473196116657892E-4
10-11	4.4697768286039446E-4
12-13	2.0980585113855252E-4
14-15	1.1858591586092099E-4
16-17	4.378556893326313E-4
18-19	0.0014686409579698675
20-21	9.760533074706573E-4
22-23	0.0010946392233315784
24-25	0.0013226890615256571
26-27	0.003429869566438945
28-29	0.003913335223410392
30-31	0.00506270640790855
32-33	0.006029637721851443
34-35	0.0035028455146610504
36-37	0.008155062213820258
38-39	0.006175589618295655
40-41	0.00587456383187947
42-43	0.013810698201033412
44-45	0.019621408078218543
46-47	0.0179976932302767
48-49	0.021245122926160383
50-51	0.017897351301471305
52-53	0.024565528570266167
54-55	0.018654476764275646
56-57	0.014859727456726174
58-59	0.016903054006945122
60-61	0.01545265703603078
62-63	0.010973758213899072
64-65	0.011001124194482362
66-67	0.013500550421089465
68-69	0.005153926343186181
70-71	0.008784479767235915
72-73	0.008410478032597627
74-75	0.011201808052093152
76-77	0.010572390498677494
78-79	0.012186983353091572
80-81	0.010052436867594994
82-83	0.006266809553573285
84-85	0.010581512492205257
86-87	0.009067261566596574
88-89	0.007999988323848285
90-91	0.012816400906507229
92-93	0.008602039896680652
94-95	0.007343204789849337
96-97	0.009286189411262889
98-99	0.009222335456568547
100	0.2605241351529156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5481258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.37074721928458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.81303140239747	48.25213017295239
2	15.267864568885473	19.961434306217672
3	5.455320587889342	10.698551494532191
4	2.358991053412208	6.168360313806532
5	1.2423845984360775	4.060780476674859
6	0.6703892527914967	2.62943078296548
7	0.375624767682409	1.7188410216228478
8	0.2467385656537291	1.290358752367902
9	0.15200979252325425	0.8943294349704194
>10	0.4141634347121369	3.758500204774376
>50	0.0023982692748340372	0.10000394474225588
>100	7.545587556177224E-4	0.09907590278150985
>500	1.234303690939813E-4	0.04790139082916049
>1k	2.057172168878073E-4	0.32030180076240167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	9.121993527763152E-6
14-15	0.0	0.0	0.0	0.0	1.8243987055526304E-5
16-17	0.0	0.0	0.0	0.0	1.8243987055526304E-5
18-19	0.0	0.0	0.0	9.121993527763152E-6	1.8243987055526304E-5
20-21	0.0	0.0	0.0	4.560996763881576E-5	1.8243987055526304E-5
22-23	0.0	0.0	0.0	1.1858591586092097E-4	1.8243987055526304E-5
24-25	0.0	0.0	0.0	2.3717183172184195E-4	1.8243987055526304E-5
26-27	0.0	0.0	0.0	4.925876504992102E-4	4.560996763881576E-5
28-29	0.0	0.0	0.0	0.002827817993606577	5.473196116657891E-5
30-31	0.0	0.0	0.0	0.010681854421010651	5.473196116657891E-5
32-33	0.0	0.0	0.0	0.025112848181931956	7.297594822210522E-5
34-35	0.0	0.0	0.0	0.04767153817609023	7.297594822210522E-5
36-37	0.0	0.0	0.0	0.08531800546516877	7.297594822210522E-5
38-39	0.0	0.0	0.0	0.1406885061786911	7.297594822210522E-5
40-41	0.0	0.0	0.0	0.19961658436804106	7.297594822210522E-5
42-43	0.0	0.0	0.0	0.2645104463245481	7.297594822210522E-5
44-45	0.0	0.0	0.0	0.3421842212134514	7.297594822210522E-5
46-47	0.0	0.0	0.0	0.43806549518376986	7.297594822210522E-5
48-49	0.0	0.0	0.0	0.5536593971675845	7.297594822210522E-5
50-51	0.0	0.0	0.0	0.6767242118506371	7.297594822210522E-5
52-53	0.0	0.0	0.0	0.7969612085400833	7.297594822210522E-5
54-55	0.0	0.0	0.0	0.9296588483884538	7.297594822210522E-5
56-57	0.0	0.0	0.0	1.0941192697004958	7.297594822210522E-5
58-59	0.0	0.0	0.0	1.2941372217837583	7.297594822210522E-5
60-61	0.0	0.0	0.0	1.5001483236147615	7.297594822210522E-5
62-63	0.0	0.0	0.0	1.719468414002771	7.297594822210522E-5
64-65	0.0	0.0	0.0	1.947034786539878	7.297594822210522E-5
66-67	0.0	0.0	0.0	2.207805215518044	7.297594822210522E-5
68-69	0.0	0.0	0.0	2.501788822930794	7.297594822210522E-5
70-71	0.0	0.0	0.0	2.833373287664985	7.297594822210522E-5
72-73	0.0	0.0	0.0	3.182280418108398	8.209794174986838E-5
74-75	0.0	0.0	0.0	3.521509113418854	9.121993527763153E-5
76-77	0.0	0.0	0.0	3.916774214970359	9.121993527763153E-5
78-79	0.0	0.0	0.0	4.343154436445064	9.121993527763153E-5
80-81	0.0	0.0	0.0	4.8144422320569475	9.121993527763153E-5
82-83	0.0	0.0	0.0	5.309410722866904	9.121993527763153E-5
84-85	0.0	0.0	0.0	5.785077075372113	9.121993527763153E-5
86-87	0.0	0.0	0.0	6.290417272823136	9.121993527763153E-5
88	0.0	0.0	0.0	6.698316335410594	9.121993527763153E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6315	0.0	44.105682	94
GTACTAG	6955	0.0	37.517044	1
TAGGCAT	8530	0.0	33.550797	5
CAACGCA	10665	0.0	33.399727	5
GTATCAA	12295	0.0	33.38247	1
GCATCGT	7320	0.0	33.2532	8
CTGTGCG	8955	0.0	32.954052	9
TAGTACT	9425	0.0	31.414736	4
ATCAACG	11430	0.0	31.249393	3
ACTGTGC	9630	0.0	31.083359	8
TATTCTC	3220	0.0	30.939592	5
CTAGGCA	9610	0.0	30.565453	4
GTACTGT	10045	0.0	29.752668	6
AGGGCTA	7720	0.0	29.21854	5
TACTGTG	11195	0.0	28.8788	7
CTAACGC	3310	0.0	28.823202	3
TCTAACG	3340	0.0	28.705023	2
CGCCTAC	3295	0.0	28.522615	7
GGTATCA	7855	0.0	28.490068	1
TAACGCC	3360	0.0	28.394287	4
>>END_MODULE