FastQCFastQC Report
Mon 16 Jan 2017
ERR1146662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481258
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177700.32419564997670247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145650.26572367146374065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.17260636153233436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126150.046.1715281
GGGCACC61500.044.58080794
GTGGTAT25650.044.3883361
GTATCAA222900.040.736911
CAGTACG9000.035.5175139
CAACGCA264100.032.974955
GTACTAG66250.032.8803141
ATCAACG265550.032.8461463
CTGTGCG89500.032.3544359
ACTGTGC94750.031.6462698
TAGTACT92800.031.5328674
TATCAAC291050.031.1336152
TAGGCAT82650.030.1945615
GTACTGT99250.029.3579926
AACGCAG305350.029.3352746
TATTCTC34850.029.2640235
TACTGTG108900.028.8282157
CTAGTAC108800.027.8902133
CCGTGTT7300.027.6899229
ACGCAGA314400.027.6091797
Produced by FastQC (version 0.11.5)