##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5481258
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1497501121093	33.0	31.0	34.0	30.0	34.0
2	32.21180101356295	33.0	31.0	34.0	30.0	34.0
3	32.36297433910245	34.0	31.0	34.0	30.0	34.0
4	35.96538239944188	37.0	35.0	37.0	35.0	37.0
5	35.87910439537785	37.0	35.0	37.0	35.0	37.0
6	35.92007966054508	37.0	35.0	37.0	35.0	37.0
7	35.84333085579989	37.0	35.0	37.0	35.0	37.0
8	35.84943547630854	37.0	35.0	37.0	35.0	37.0
9	37.555138437198174	39.0	37.0	39.0	35.0	39.0
10-11	37.49269383050387	39.0	37.0	39.0	34.5	39.0
12-13	37.457257257366834	39.0	37.0	39.0	34.0	39.0
14-15	38.65544077655166	40.0	38.0	41.0	34.0	41.0
16-17	38.662493080967906	40.0	38.0	41.0	34.0	41.0
18-19	38.73554264367778	40.0	38.0	41.0	34.0	41.0
20-21	38.73788106671863	40.0	38.0	41.0	34.0	41.0
22-23	38.698058000553885	40.0	38.0	41.0	34.0	41.0
24-25	38.61175500222759	40.0	38.0	41.0	34.0	41.0
26-27	38.45963034398308	40.0	38.0	41.0	34.0	41.0
28-29	38.40275015334071	40.0	38.0	41.0	34.0	41.0
30-31	38.257607742602154	40.0	37.5	41.0	34.0	41.0
32-33	38.09710316500336	40.0	37.0	41.0	33.0	41.0
34-35	37.98752512653117	40.0	37.0	41.0	33.0	41.0
36-37	37.968627457419444	40.0	37.0	41.0	33.0	41.0
38-39	37.864903823173435	40.0	37.0	41.0	33.0	41.0
40-41	37.73861064376098	40.0	37.0	41.0	33.0	41.0
42-43	37.59366964663951	40.0	36.0	41.0	32.5	41.0
44-45	37.35401854829676	40.0	36.0	41.0	32.0	41.0
46-47	37.06651310702762	40.0	35.5	41.0	31.0	41.0
48-49	36.75245947189495	39.0	35.0	41.0	30.5	41.0
50-51	36.52347681864273	39.0	35.0	41.0	30.0	41.0
52-53	36.32983596101479	39.0	35.0	41.0	30.0	41.0
54-55	36.13965443699239	38.5	35.0	41.0	30.0	41.0
56-57	35.89867143637464	38.0	35.0	40.0	29.0	41.0
58-59	35.60208568908816	37.0	35.0	40.0	29.0	41.0
60-61	35.416614032764016	37.0	35.0	40.0	29.0	41.0
62-63	35.19735013385613	36.5	34.0	40.0	29.0	41.0
64-65	34.950305203659454	36.0	34.0	39.5	29.0	41.0
66-67	34.70643107841302	35.5	34.0	39.0	28.5	41.0
68-69	34.46745117635404	35.0	34.0	39.0	28.0	41.0
70-71	34.27544525362609	35.0	34.0	38.0	28.0	41.0
72-73	34.05364516685768	35.0	34.0	37.5	28.0	40.0
74-75	33.80524242792439	35.0	34.0	37.0	28.0	39.5
76-77	33.57506424620041	35.0	33.5	36.5	27.0	39.0
78-79	33.36090574463016	35.0	33.0	36.0	27.0	39.0
80-81	33.243198732845634	35.0	33.0	36.0	27.0	38.5
82-83	33.087617203933846	35.0	33.0	35.5	27.0	37.0
84-85	32.96185611040386	35.0	33.0	35.0	27.0	37.0
86-87	32.82510237613336	35.0	33.0	35.0	27.0	36.5
88-89	32.72337600237026	35.0	33.0	35.0	27.0	36.0
90-91	32.656862348023026	35.0	33.0	35.0	27.0	36.0
92-93	32.587023362155186	35.0	33.0	35.0	27.0	36.0
94-95	32.54252445332805	35.0	33.0	35.0	27.0	35.5
96-97	32.45266369508606	35.0	33.0	35.0	27.0	35.0
98-99	32.38207743550842	35.0	33.0	35.0	27.0	35.0
100	31.168666937407433	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.337305631671839
1101	2	0.4002723117773357
1101	3	0.32688314065302393
1101	4	0.2322332661071016
1101	5	0.1870901363418085
1101	6	0.22967477905193334
1101	7	0.32011594278856137
1101	8	0.27715453562597503
1101	9	0.3225411374332978
1101	10-11	0.37041939792991485
1101	12-13	0.3633563378911049
1101	14-15	0.4887481046564517
1101	16-17	0.4750135362410788
1101	18-19	0.4835881326348499
1101	20-21	0.4949797942108276
1101	22-23	0.4987600649549506
1101	24-25	0.4983484974513601
1101	26-27	0.5663323349307063
1101	28-29	0.563715493972822
1101	30-31	0.5572125467168902
1101	32-33	0.5699604919891783
1101	34-35	0.5722826365801126
1101	36-37	0.554018247903457
1101	38-39	0.5812645584158318
1101	40-41	0.6101203671623452
1101	42-43	0.5255607580718831
1101	44-45	0.5081636587633795
1101	46-47	0.5763778953216345
1101	48-49	0.46352949343630456
1101	50-51	0.4793308538799508
1101	52-53	0.5321164667805007
1101	54-55	0.5919675339063986
1101	56-57	0.6075690192255223
1101	58-59	0.5750383117136977
1101	60-61	0.5680942064429075
1101	62-63	0.5335561822613286
1101	64-65	0.5502814775976645
1101	66-67	0.5548542637869076
1101	68-69	0.539184677924716
1101	70-71	0.5169514120404202
1101	72-73	0.5277658957679421
1101	74-75	0.5373989880060037
1101	76-77	0.42490563676790316
1101	78-79	0.3835205796237062
1101	80-81	0.4327558721134821
1101	82-83	0.46775645214056283
1101	84-85	0.3842634415821209
1101	86-87	0.33940033141258397
1101	88-89	0.37429067067065347
1101	90-91	0.4409948671568884
1101	92-93	0.4085406837923955
1101	94-95	0.41257204111850854
1101	96-97	0.4266509181529088
1101	98-99	0.46035359729020087
1101	100	0.742955885905836
1102	1	0.31097834434258687
1102	2	0.3725617219568562
1102	3	0.30703030549464216
1102	4	0.22239450667858307
1102	5	0.23782172065310192
1102	6	0.21126451731465323
1102	7	0.25980777991509285
1102	8	0.26359898463721265
1102	9	0.34784567469490213
1102	10-11	0.32201416041658604
1102	12-13	0.3550323599133023
1102	14-15	0.44063519599847467
1102	16-17	0.46170213684418115
1102	18-19	0.46915381777877485
1102	20-21	0.46879768346042994
1102	22-23	0.4940184649642205
1102	24-25	0.5138498047762852
1102	26-27	0.5560550532863431
1102	28-29	0.536716328907616
1102	30-31	0.4940906927777533
1102	32-33	0.5146282352760494
1102	34-35	0.5312341118418189
1102	36-37	0.5019694271081079
1102	38-39	0.5340699771775164
1102	40-41	0.5324326036763978
1102	42-43	0.5184120308750124
1102	44-45	0.511528371080388
1102	46-47	0.5119780543231371
1102	48-49	0.5760016214261938
1102	50-51	0.5455610092989147
1102	52-53	0.5310142754804943
1102	54-55	0.46160805927135584
1102	56-57	0.4787111905450345
1102	58-59	0.49788178770293
1102	60-61	0.47728904219076185
1102	62-63	0.5008899061820955
1102	64-65	0.4281911491303987
1102	66-67	0.4355538105486403
1102	68-69	0.4631281049877387
1102	70-71	0.4049597175096764
1102	72-73	0.4716945627987812
1102	74-75	0.4404748235653244
1102	76-77	0.41755979446327274
1102	78-79	0.40307112477325546
1102	80-81	0.3465893153115829
1102	82-83	0.3237678049126629
1102	84-85	0.34245181808819325
1102	86-87	0.40454298513298426
1102	88-89	0.3730326141847726
1102	90-91	0.42222673134766353
1102	92-93	0.37775875209145937
1102	94-95	0.3488775159189146
1102	96-97	0.37444810040464205
1102	98-99	0.39246722030754455
1102	100	0.6229457108148786
1103	1	0.27110756977793926
1103	2	0.3656810251602636
1103	3	0.2752431091799039
1103	4	0.22120191904811293
1103	5	0.1865650933813825
1103	6	0.22561835258154161
1103	7	0.2026976024429885
1103	8	0.18911230340446394
1103	9	0.2933286077054831
1103	10-11	0.311689970780499
1103	12-13	0.2988392575112684
1103	14-15	0.3867017726810502
1103	16-17	0.4034827838749564
1103	18-19	0.36209758556789495
1103	20-21	0.3510197833362767
1103	22-23	0.3300403437770001
1103	24-25	0.34294796182975773
1103	26-27	0.4559084853329338
1103	28-29	0.447788242535232
1103	30-31	0.38326573117732465
1103	32-33	0.4074717026286834
1103	34-35	0.45677961488960506
1103	36-37	0.42068913642856387
1103	38-39	0.39841456167531675
1103	40-41	0.35724050445566036
1103	42-43	0.40637296916276
1103	44-45	0.44580731428243325
1103	46-47	0.41481371944056633
1103	48-49	0.4187557247249316
1103	50-51	0.36197699162338637
1103	52-53	0.3656808438411545
1103	54-55	0.32513473520664604
1103	56-57	0.2670209832726371
1103	58-59	0.28958373282852534
1103	60-61	0.38685465589424695
1103	62-63	0.33634124182089664
1103	64-65	0.38773150356184516
1103	66-67	0.4179458405305567
1103	68-69	0.3395443038041819
1103	70-71	0.34418298488195376
1103	72-73	0.34117035260400286
1103	74-75	0.2573224709177424
1103	76-77	0.2968415683861849
1103	78-79	0.29074249299875277
1103	80-81	0.23465121348383633
1103	82-83	0.2467326546118329
1103	84-85	0.2497637118654623
1103	86-87	0.23888250748255757
1103	88-89	0.2508189449498488
1103	90-91	0.26053620319750337
1103	92-93	0.24970647478338748
1103	94-95	0.2406069503767725
1103	96-97	0.29029194883781173
1103	98-99	0.2586374705836789
1103	100	0.5123392597307799
1104	1	0.20603999373582127
1104	2	0.211754133880369
1104	3	0.1704952984260899
1104	4	0.1422724977377996
1104	5	0.12193203484459758
1104	6	0.17052865311637078
1104	7	0.1886169785736982
1104	8	0.1799178251427307
1104	9	0.25438205396339697
1104	10-11	0.2163866493769433
1104	12-13	0.2539734511361047
1104	14-15	0.3322716874356999
1104	16-17	0.28983214661205636
1104	18-19	0.28048038789880536
1104	20-21	0.3269038071558512
1104	22-23	0.34040897259028213
1104	24-25	0.3434262446041032
1104	26-27	0.37901110137722327
1104	28-29	0.34708803483481176
1104	30-31	0.3172527377941421
1104	32-33	0.2664790636763854
1104	34-35	0.3653174995308248
1104	36-37	0.31229611641923327
1104	38-39	0.2832614308981576
1104	40-41	0.36953705702722317
1104	42-43	0.35445723045108934
1104	44-45	0.3437684702349131
1104	46-47	0.3681359990992732
1104	48-49	0.3460601408013133
1104	50-51	0.3419261484759346
1104	52-53	0.33020205108206113
1104	54-55	0.38095909439199716
1104	56-57	0.3898137237062471
1104	58-59	0.2882390170876903
1104	60-61	0.27071569253941874
1104	62-63	0.27747767116665045
1104	64-65	0.26815802491381646
1104	66-67	0.22585461614017532
1104	68-69	0.2658211113642963
1104	70-71	0.254772211340061
1104	72-73	0.1872274432356491
1104	74-75	0.25104785225765625
1104	76-77	0.19590465423901549
1104	78-79	0.20239389457830725
1104	80-81	0.17769854587690048
1104	82-83	0.19777347466920503
1104	84-85	0.2034184722941461
1104	86-87	0.24873430029814614
1104	88-89	0.21609573170952956
1104	90-91	0.18197488758615066
1104	92-93	0.2210912459307437
1104	94-95	0.19306332225220046
1104	96-97	0.18956958795396162
1104	98-99	0.1727229213642687
1104	100	0.4015408607502593
1105	1	0.1530065828022913
1105	2	0.17137724997895276
1105	3	0.10390869656897905
1105	4	0.1690042774549454
1105	5	0.1331086070439369
1105	6	0.12056087062909882
1105	7	0.20654006440823736
1105	8	0.17664197908595014
1105	9	0.1530540308857553
1105	10-11	0.16444876204833037
1105	12-13	0.1725707852871068
1105	14-15	0.23450507157354394
1105	16-17	0.2130353357921848
1105	18-19	0.19457503008150923
1105	20-21	0.2244979111549057
1105	22-23	0.20078090843271923
1105	24-25	0.2090363329245264
1105	26-27	0.1834703878023234
1105	28-29	0.2043435135183671
1105	30-31	0.2615338212081113
1105	32-33	0.2686781563850218
1105	34-35	0.20021623573274638
1105	36-37	0.24021590591562614
1105	38-39	0.2513790880872051
1105	40-41	0.2394863098505482
1105	42-43	0.25077942756840343
1105	44-45	0.2982927476124573
1105	46-47	0.29130803320489207
1105	48-49	0.32034406284021344
1105	50-51	0.2972674068778005
1105	52-53	0.26819902856885136
1105	54-55	0.24715626476690744
1105	56-57	0.22467142168736842
1105	58-59	0.21343205423815448
1105	60-61	0.20042099595617913
1105	62-63	0.2069624491601445
1105	64-65	0.22351488015596033
1105	66-67	0.2016372864322733
1105	68-69	0.21528424495851795
1105	70-71	0.21366097878291868
1105	72-73	0.19253122598210837
1105	74-75	0.22759960892051367
1105	76-77	0.16078989716659464
1105	78-79	0.21177793427915503
1105	80-81	0.11816519036619866
1105	82-83	0.16067784167020704
1105	84-85	0.18952869074780665
1105	86-87	0.1806812866071894
1105	88-89	0.17039873470662315
1105	90-91	0.20954745281205334
1105	92-93	0.16909615989285953
1105	94-95	0.19204755100527393
1105	96-97	0.2673265309955042
1105	98-99	0.2227269472021547
1105	100	0.4044287161022595
1106	1	0.13984649346191702
1106	2	0.2312879387473643
1106	3	0.16045740532130282
1106	4	0.13189098931000132
1106	5	0.08514939809476374
1106	6	0.12323092098810662
1106	7	0.13110111697868376
1106	8	0.05945059736139058
1106	9	0.11789775080116982
1106	10-11	0.15468919816294857
1106	12-13	0.1527662279836619
1106	14-15	0.19041374316287119
1106	16-17	0.16705285738157727
1106	18-19	0.19168710320879256
1106	20-21	0.17972316505002794
1106	22-23	0.18961888529332782
1106	24-25	0.20525105931317
1106	26-27	0.21189372011401986
1106	28-29	0.17809661949273448
1106	30-31	0.194267555025462
1106	32-33	0.22318167760193575
1106	34-35	0.2165400692793753
1106	36-37	0.14681045734945286
1106	38-39	0.1861550090659776
1106	40-41	0.1844381300591067
1106	42-43	0.21066084410532682
1106	44-45	0.15824190599268206
1106	46-47	0.186637399482791
1106	48-49	0.16035021256613646
1106	50-51	0.1631140248452354
1106	52-53	0.20631266489396438
1106	54-55	0.1524989034623161
1106	56-57	0.14532097827282797
1106	58-59	0.1471091216958058
1106	60-61	0.11120481585808761
1106	62-63	0.20087700528871721
1106	64-65	0.08985771954040445
1106	66-67	0.10596223633212531
1106	68-69	0.17683815440866368
1106	70-71	0.18060155570696423
1106	72-73	0.1394766817105193
1106	74-75	0.1223080405156054
1106	76-77	0.1163445603083062
1106	78-79	0.1625609694449892
1106	80-81	0.08992041599031353
1106	82-83	0.08691077756351717
1106	84-85	0.11962526234921
1106	86-87	0.07655614704444957
1106	88-89	0.07978668190396121
1106	90-91	0.045444889205093375
1106	92-93	0.08369038785723149
1106	94-95	0.1206123335550302
1106	96-97	0.05226441418437133
1106	98-99	0.03869359648409443
1106	100	0.21982804491651464
1107	1	0.09884705711628072
1107	2	0.1518393857889393
1107	3	0.12213706072053299
1107	4	0.11840285459764743
1107	5	0.03319577345812519
1107	6	-0.007796704995911341
1107	7	0.11670345380870373
1107	8	0.08750573376125459
1107	9	0.10725209493620724
1107	10-11	0.14615583096652784
1107	12-13	-0.0024582666564327837
1107	14-15	0.1220853745322259
1107	16-17	0.13292188680613037
1107	18-19	0.0923013653596314
1107	20-21	0.1526598527208094
1107	22-23	0.10554389608026327
1107	24-25	0.08114961398435128
1107	26-27	0.12183249320214173
1107	28-29	0.1825346657815956
1107	30-31	0.09211408394916276
1107	32-33	0.1744639065037319
1107	34-35	0.18883646773906548
1107	36-37	0.16481320399667965
1107	38-39	0.1926068757861188
1107	40-41	0.27271840295224337
1107	42-43	0.23914484915849243
1107	44-45	0.204769549450333
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2216	68-69	-0.7026741962305962
2216	70-71	-0.6853519490170399
2216	72-73	-0.6674961588109127
2216	74-75	-0.6244887846991816
2216	76-77	-0.6949661925107762
2216	78-79	-0.6558027848461307
2216	80-81	-0.7103329520304982
2216	82-83	-0.7163064815848657
2216	84-85	-0.6873683851786012
2216	86-87	-0.6584505264959546
2216	88-89	-0.6190483033975127
2216	90-91	-0.659269534293152
2216	92-93	-0.6531232566730836
2216	94-95	-0.6266813183105988
2216	96-97	-0.653997913643046
2216	98-99	-0.6831409589334072
2216	100	-0.07423133700892492
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	7.0
12	27.0
13	94.0
14	345.0
15	1021.0
16	2466.0
17	5044.0
18	9077.0
19	14262.0
20	20739.0
21	29798.0
22	41383.0
23	49325.0
24	49427.0
25	46423.0
26	45354.0
27	52775.0
28	65366.0
29	82532.0
30	102236.0
31	128492.0
32	162936.0
33	210391.0
34	303055.0
35	446312.0
36	708348.0
37	1328353.0
38	1112306.0
39	462411.0
40	949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.42795613707656	22.450868030660114	13.632509179462089	24.488666652801236
2	14.124147720534438	20.74372957676582	40.600249229595924	24.531873473103822
3	16.851076887823925	27.949459777299303	29.979449972980653	25.22001336189612
4	11.373417008429657	17.697768605258226	39.805023902257396	31.123790484054727
5	12.060744334296357	39.22926543580967	35.474315385590785	13.235674844303189
6	28.690431536129672	40.00411584498151	17.704847210201457	13.600605408687352
7	25.23882308683378	33.06706822927746	24.432109878505905	17.261998805382856
8	26.47233536534861	34.642339404567345	19.5701059866184	19.31521924346564
9	26.38383378414225	14.116066056368812	21.968551744873167	37.53154841461577
10-11	25.515739270072675	26.195300421910446	27.446682860029576	20.842277447987307
12-13	25.904440879358532	24.148352778579248	28.11866390585862	21.828542436203595
14-15	23.150527518851426	24.888865615521375	26.240669163647617	25.719937701979585
16-17	21.049346701067527	27.18406066636528	27.69881476113695	24.067777871430245
18-19	20.407685942640676	27.21742389765407	30.83611257899311	21.53877758071215
20-21	22.16496650951296	26.438182621580665	29.90112853655128	21.49572233235509
22-23	22.71860766269349	25.998575509490706	29.635952184699203	21.646864643116597
24-25	22.101486556553258	26.04662104940143	30.534304351300378	21.31758804274493
26-27	22.02401547455123	26.365382396284065	29.330778566779614	22.279823562385094
28-29	21.005196252393155	26.96897318097415	29.96675215799001	22.059078408642687
30-31	22.0861889734072	26.421088005709635	29.867094378699193	21.625628642183965
32-33	22.786028316857188	26.733315600177914	28.878507452121394	21.602148630843505
34-35	22.389896625920546	26.606392182232618	29.8511947439803	21.152516447866528
36-37	22.069203821458505	26.074470495641695	29.49965135740737	22.35667432549243
38-39	22.275132825347757	26.075391816988	30.34507771756046	21.304397640103787
40-41	21.742280695417	25.27085935381987	30.78002349095773	22.206836459805395
42-43	21.994941672148986	25.560838406073934	29.723368248675758	22.720851673101322
44-45	22.24748406296511	26.784526471842778	29.25542822468857	21.712561240503547
46-47	22.296934389879112	26.49139120982811	28.381194608974802	22.830479791317977
48-49	23.384741240058396	26.251583121976747	28.288396568816864	22.075279069147996
50-51	22.634539370341624	26.309845294638567	27.865190801089824	23.190424533929985
52-53	21.74896711667285	27.07409503438809	27.753355160439448	23.42358268849961
54-55	21.990946238983824	27.039540922904926	27.475754653402557	23.493758184708692
56-57	23.313042370930177	26.308613825512317	27.218423215984362	23.159920587573144
58-59	21.94950702461981	26.827329312662286	28.33121779080804	22.891945871909865
60-61	22.210047401527166	27.544497996627783	27.286427677733833	22.959026924111217
62-63	22.76397133650706	26.593703489235498	26.813050945604093	23.82927422865335
64-65	21.695445969115827	26.868961007827767	27.338488601331008	24.097104421725394
66-67	22.496971037075674	26.30941414995013	27.34380430445689	23.84981050851731
68-69	21.916291843952614	26.205772470480316	27.846426860403213	24.031508825163858
70-71	21.989599957308833	25.867271519016498	27.860310487021383	24.282818036653286
72-73	22.69401793355619	25.902037005177487	28.3466603225953	23.057284738671026
74-75	22.82862893129035	26.508157883862417	27.657397794637937	23.0058153902093
76-77	22.482947326632676	26.450015434429947	27.153659419417313	23.913377819520065
78-79	22.094010552688918	26.52710865409157	27.390040277257572	23.98884051596194
80-81	21.71237880063299	27.035463391798014	28.040843908460428	23.211313899108564
82-83	22.69646858440161	25.811364836320422	29.207501270693697	22.28466530858427
84-85	23.1431817294497	26.540604364910394	27.3224595521685	22.99375435347141
86-87	21.43683074213985	27.276785730574986	27.952816670917514	23.333566856367643
88-89	22.61943334905965	25.89390063375962	27.709742909383213	23.77692310779752
90-91	22.12053327903923	25.854329425836188	28.044957927541454	23.980179367583133
92-93	22.544058316539743	26.342191883688017	27.53521180721652	23.578537992555724
94-95	21.127649893509847	26.382356021190756	28.327028211406947	24.162965873892453
96-97	21.66346667133713	26.21765842804699	27.789961720466362	24.32891318014952
98-99	20.875071014719616	27.094966155579613	27.679248085019896	24.350714744680875
100	21.83153513335889	26.339175201270603	26.536064406594008	25.29322525877649
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	753.0
1	481.5
2	303.5
3	398.5
4	277.0
5	208.5
6	405.0
7	902.5
8	1524.5
9	1695.0
10	1275.5
11	887.0
12	898.0
13	1080.5
14	1371.5
15	1963.0
16	2663.5
17	3575.5
18	4974.0
19	6601.0
20	8344.0
21	10176.5
22	12259.0
23	14234.0
24	15892.0
25	17866.0
26	20576.5
27	23039.5
28	27557.0
29	38998.0
30	55091.5
31	68776.0
32	88758.5
33	107924.0
34	118973.0
35	134706.5
36	149785.5
37	177512.5
38	187240.0
39	167829.0
40	151130.0
41	135641.0
42	131795.5
43	139664.0
44	154167.5
45	191621.0
46	224879.5
47	245001.5
48	259796.5
49	251367.0
50	235743.0
51	241920.5
52	246987.0
53	235550.0
54	226657.0
55	193748.5
56	164302.5
57	133677.0
58	100407.0
59	99565.0
60	82969.0
61	51956.0
62	35861.0
63	30635.5
64	21987.0
65	8053.0
66	3218.5
67	1934.5
68	1349.5
69	914.0
70	541.0
71	384.5
72	241.0
73	120.0
74	42.5
75	18.0
76	7.0
77	3.0
78	1.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0067137872364336805
3	0.0
4	0.09116520331646494
5	0.004615728725048155
6	3.648797411105261E-5
7	7.297594822210522E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.280498381940788E-4
14-15	0.00923145745009631
16-17	0.0
18-19	8.209794174986837E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.121993527763152E-6
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	9.121993527763152E-6
60-61	0.0
62-63	0.0
64-65	7.297594822210522E-5
66-67	1.6419588349973673E-4
68-69	0.0
70-71	5.564416051935522E-4
72-73	4.560996763881576E-4
74-75	4.8346565697144706E-4
76-77	1.0946392233315783E-4
78-79	1.8243987055526304E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06491210594356259
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5481258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.86303936959776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8233929969297	44.432133886271544
2	16.29789863077656	20.164750892748877
3	5.876661419590753	10.90644410285817
4	2.602729677478483	6.440510740250875
5	1.325064547990667	4.098626014980245
6	0.7228545202474601	2.6830786586736197
7	0.42766688591683266	1.8519741380382406
8	0.2631697255134235	1.3024383272258524
9	0.17392914547599775	0.9683807016691932
>10	0.4747712820174661	4.120136838945689
>50	0.005069321459781968	0.22097396343498957
>100	0.005228642317296788	0.6924531156005032
>500	8.25024506688326E-4	0.36186312654725145
>1k	6.079127590284399E-4	0.6334954897694859
>5k	4.3422339930602464E-5	0.2541449700508448
>10k+	8.684467986120493E-5	0.8685950329345805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17770	0.32419564997670247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14565	0.26572367146374065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9461	0.17260636153233436	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8243987055526304E-5
2	0.0	0.0	0.0	0.0	1.8243987055526304E-5
3	0.0	0.0	0.0	0.0	1.8243987055526304E-5
4	0.0	0.0	0.0	0.0	1.8243987055526304E-5
5	0.0	0.0	0.0	0.0	1.8243987055526304E-5
6	0.0	0.0	0.0	0.0	1.8243987055526304E-5
7	0.0	0.0	0.0	0.0	1.8243987055526304E-5
8	0.0	0.0	0.0	0.0	1.8243987055526304E-5
9	0.0	0.0	0.0	0.0	1.8243987055526304E-5
10-11	0.0	0.0	0.0	0.0	1.8243987055526304E-5
12-13	0.0	0.0	0.0	0.0	3.648797411105261E-5
14-15	0.0	0.0	0.0	0.0	4.560996763881576E-5
16-17	0.0	0.0	0.0	0.0	5.473196116657891E-5
18-19	0.0	0.0	0.0	9.121993527763152E-6	7.297594822210522E-5
20-21	0.0	0.0	0.0	4.560996763881576E-5	7.297594822210522E-5
22-23	0.0	0.0	0.0	1.1858591586092097E-4	7.297594822210522E-5
24-25	0.0	0.0	0.0	2.3717183172184195E-4	7.297594822210522E-5
26-27	0.0	0.0	0.0	4.925876504992102E-4	7.297594822210522E-5
28-29	0.0	0.0	0.0	0.0029190379288842085	7.297594822210522E-5
30-31	0.0	0.0	0.0	0.010800440336871572	7.297594822210522E-5
32-33	0.0	0.0	0.0	0.025213190110737353	7.297594822210522E-5
34-35	0.0	0.0	0.0	0.04795431997545089	7.297594822210522E-5
36-37	0.0	0.0	0.0	0.08599303298622324	7.297594822210522E-5
38-39	0.0	0.0	0.0	0.14156421755735635	7.297594822210522E-5
40-41	0.0	0.0	0.0	0.20089366346192788	7.297594822210522E-5
42-43	0.0	0.0	0.0	0.26590611133429587	7.297594822210522E-5
44-45	0.0	0.0	0.0	0.34440086564069783	7.297594822210522E-5
46-47	0.0	0.0	0.0	0.4407747272615155	7.297594822210522E-5
48-49	0.0	0.0	0.0	0.5567973629411351	7.297594822210522E-5
50-51	0.0	0.0	0.0	0.6801084714494373	9.121993527763153E-5
52-53	0.0	0.0	0.0	0.8012394235046043	1.2770790938868414E-4
54-55	0.0	0.0	0.0	0.9347124328028347	1.2770790938868414E-4
56-57	0.0	0.0	0.0	1.100349591279958	1.2770790938868414E-4
58-59	0.0	0.0	0.0	1.3024108699134396	1.2770790938868414E-4
60-61	0.0	0.0	0.0	1.5103558343723282	1.2770790938868414E-4
62-63	0.0	0.0	0.0	1.7312631516341686	1.2770790938868414E-4
64-65	0.0	0.0	0.0	1.9612468524561333	1.2770790938868414E-4
66-67	0.0	0.0	0.0	2.2242065598809617	1.3682990291644727E-4
68-69	0.0	0.0	0.0	2.5205983735850417	1.4595189644421043E-4
70-71	0.0	0.0	0.0	2.8548738264099227	1.4595189644421043E-4
72-73	0.0	0.0	0.0	3.20628950507347	1.550738899719736E-4
74-75	0.0	0.0	0.0	3.5489480699503653	1.6419588349973673E-4
76-77	0.0	0.0	0.0	3.9483363125764193	1.6419588349973673E-4
78-79	0.0	0.0	0.0	4.379478214672616	1.6419588349973673E-4
80-81	0.0	0.0	0.0	4.856147986465881	1.6419588349973673E-4
82-83	0.0	0.0	0.0	5.357219090945911	1.8243987055526306E-4
84-85	0.0	0.0	0.0	5.838504591464222	1.8243987055526306E-4
86-87	0.0	0.0	0.0	6.349582522844209	1.915618640830262E-4
88	0.0	0.0	0.0	6.762261510040213	2.0068385761078935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12615	0.0	46.171528	1
GGGCACC	6150	0.0	44.580807	94
GTGGTAT	2565	0.0	44.388336	1
GTATCAA	22290	0.0	40.73691	1
CAGTACG	900	0.0	35.517513	9
CAACGCA	26410	0.0	32.97495	5
GTACTAG	6625	0.0	32.880314	1
ATCAACG	26555	0.0	32.846146	3
CTGTGCG	8950	0.0	32.354435	9
ACTGTGC	9475	0.0	31.646269	8
TAGTACT	9280	0.0	31.532867	4
TATCAAC	29105	0.0	31.133615	2
TAGGCAT	8265	0.0	30.194561	5
GTACTGT	9925	0.0	29.357992	6
AACGCAG	30535	0.0	29.335274	6
TATTCTC	3485	0.0	29.264023	5
TACTGTG	10890	0.0	28.828215	7
CTAGTAC	10880	0.0	27.890213	3
CCGTGTT	730	0.0	27.689922	9
ACGCAGA	31440	0.0	27.609179	7
>>END_MODULE