FastQCFastQC Report
Mon 16 Jan 2017
ERR1146661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5415377
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC51100.037.81212294
GTACTAG61450.030.5291631
TAGTACT69000.029.6956444
CTGTGCG69800.029.487779
TAGGCAT72550.028.8892825
TATTCTC30150.028.8352475
TACCCCG3750.028.8227795
CTAACGC32650.028.4995043
CGCCTAC32900.028.4239527
ACTGTGC75100.028.2204468
TCTAACG33650.028.0720632
TAACGCC33950.027.6850644
GTACATG246200.027.5576521
GTACTGT77500.027.225256
ACATGGG242950.027.1197973
TACATGG248300.027.0848372
GCATCGT61550.026.7218958
CTAGGCA84850.025.976244
CATGGGG196700.025.7315924
AGGGCTA66200.025.697445
Produced by FastQC (version 0.11.5)