##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146661_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5415377
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58212900043709	31.0	31.0	34.0	28.0	34.0
2	31.62289144412291	33.0	31.0	34.0	28.0	34.0
3	31.725520494695015	33.0	31.0	34.0	28.0	34.0
4	35.14750164208328	37.0	35.0	37.0	32.0	37.0
5	35.29143511153517	37.0	35.0	37.0	32.0	37.0
6	35.368407776596165	37.0	35.0	37.0	32.0	37.0
7	35.265409407322885	37.0	35.0	37.0	32.0	37.0
8	35.270799983085205	37.0	35.0	37.0	32.0	37.0
9	36.80652298076385	39.0	37.0	39.0	32.0	39.0
10-11	36.64983176609864	39.0	37.0	39.0	32.0	39.0
12-13	36.60832265232873	39.0	37.0	39.0	32.0	39.0
14-15	37.809528219364964	40.0	38.0	41.0	32.0	41.0
16-17	37.85923870120215	40.0	38.0	41.0	32.0	41.0
18-19	37.82930089631802	40.0	38.0	41.0	32.0	41.0
20-21	37.89278207223615	40.0	38.0	41.0	32.0	41.0
22-23	37.836188597765215	40.0	38.0	41.0	32.0	41.0
24-25	37.793976485847615	40.0	37.0	41.0	32.0	41.0
26-27	37.82685822242847	40.0	37.0	41.0	32.0	41.0
28-29	37.81789578084776	40.0	37.0	41.0	32.0	41.0
30-31	37.678671494154514	40.0	37.0	41.0	31.0	41.0
32-33	37.65899456307474	40.0	37.0	41.0	31.0	41.0
34-35	37.59123464534417	40.0	37.0	41.0	31.0	41.0
36-37	37.473269174057506	40.0	36.5	41.0	31.0	41.0
38-39	37.37374664774032	40.0	36.0	41.0	31.0	41.0
40-41	37.28325193241394	40.0	36.0	41.0	30.0	41.0
42-43	37.11860356167262	40.0	36.0	41.0	30.0	41.0
44-45	36.950121108835084	39.0	35.0	41.0	30.0	41.0
46-47	36.93569367377377	39.0	35.0	41.0	30.0	41.0
48-49	36.77850480215874	39.0	35.0	41.0	30.0	41.0
50-51	36.58416320784315	39.0	35.0	41.0	30.0	41.0
52-53	36.35315177502878	39.0	35.0	41.0	29.0	41.0
54-55	36.12697915583716	38.0	35.0	40.0	29.0	41.0
56-57	35.851269911586954	38.0	34.5	40.0	29.0	41.0
58-59	35.52689655770965	37.0	34.0	40.0	28.0	41.0
60-61	35.24474011689306	37.0	34.0	40.0	28.0	41.0
62-63	34.920039269657494	36.0	34.0	39.5	27.5	41.0
64-65	34.7616581449454	35.5	34.0	39.0	28.0	41.0
66-67	34.48921580159609	35.0	33.5	39.0	27.5	41.0
68-69	34.35493373776193	35.0	33.0	39.0	27.0	41.0
70-71	34.271302995156205	35.0	34.0	38.0	28.0	41.0
72-73	34.04697419958019	35.0	33.0	37.0	27.5	40.0
74-75	33.78484757386236	35.0	33.0	37.0	27.0	39.5
76-77	33.533543925012054	35.0	33.0	36.0	27.0	39.0
78-79	33.28279979399403	35.0	33.0	36.0	26.0	39.0
80-81	33.09308650164152	35.0	33.0	36.0	26.0	38.5
82-83	32.828843587436296	35.0	33.0	35.0	26.0	37.0
84-85	32.636227542422255	35.0	33.0	35.0	25.5	37.0
86-87	32.46391119214785	35.0	33.0	35.0	25.0	36.5
88-89	32.33803482195238	35.0	33.0	35.0	25.0	36.0
90-91	32.163500435888395	35.0	32.5	35.0	25.0	36.0
92-93	31.99604616631492	35.0	32.0	35.0	25.0	36.0
94-95	31.90188965606642	35.0	32.0	35.0	24.5	35.5
96-97	31.77647751947833	35.0	32.0	35.0	24.0	35.0
98-99	31.664629627817234	35.0	32.0	35.0	24.0	35.0
100	30.69896278689369	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4035059965821688
1101	2	0.4518089113309962
1101	3	0.5181241100512644
1101	4	0.5361601963700906
1101	5	0.41830026807239307
1101	6	0.4392202506390106
1101	7	0.41714304087993526
1101	8	0.4074653357777507
1101	9	0.592051475064153
1101	10-11	0.6155218257050592
1101	12-13	0.5408429328173128
1101	14-15	0.7264289786583973
1101	16-17	0.7153438852610208
1101	18-19	0.686889831171257
1101	20-21	0.6846000939090118
1101	22-23	0.7257750892065005
1101	24-25	0.7211830933827983
1101	26-27	0.6775119939420833
1101	28-29	0.670009329769016
1101	30-31	0.7055387791365391
1101	32-33	0.6756685493712951
1101	34-35	0.672712659271447
1101	36-37	0.6688786676574949
1101	38-39	0.6245704873764737
1101	40-41	0.6291166784829798
1101	42-43	0.6044290274943549
1101	44-45	0.641124975781878
1101	46-47	0.6433087383437979
1101	48-49	0.6095994934661846
1101	50-51	0.5793944294538207
1101	52-53	0.6133843427610799
1101	54-55	0.5660449717849332
1101	56-57	0.5282967061407149
1101	58-59	0.5359457189620684
1101	60-61	0.5603408772269347
1101	62-63	0.555415787765007
1101	64-65	0.5530337575436803
1101	66-67	0.5878076496724702
1101	68-69	0.589451031308073
1101	70-71	0.5920753801128456
1101	72-73	0.5996263760300167
1101	74-75	0.47382156204943016
1101	76-77	0.5200699619016618
1101	78-79	0.5164065067391803
1101	80-81	0.44506639541053516
1101	82-83	0.460305142991281
1101	84-85	0.49008156521686175
1101	86-87	0.5262044044126029
1101	88-89	0.5132945849961317
1101	90-91	0.5133197182159179
1101	92-93	0.5712127243148686
1101	94-95	0.556059472694777
1101	96-97	0.5816161692091555
1101	98-99	0.5773464086531206
1101	100	0.6206856911398972
1102	1	0.3037923026573033
1102	2	0.3613419565346483
1102	3	0.3777000099859045
1102	4	0.4584265969079979
1102	5	0.3252027328223903
1102	6	0.3778330985873666
1102	7	0.3601198357931352
1102	8	0.41755446302953914
1102	9	0.5263155531521235
1102	10-11	0.5774091162919035
1102	12-13	0.5424878838711535
1102	14-15	0.6488080309599908
1102	16-17	0.6079607533772773
1102	18-19	0.6476490746455497
1102	20-21	0.5930845070815707
1102	22-23	0.6683407493017128
1102	24-25	0.6436303250024693
1102	26-27	0.5881376141885752
1102	28-29	0.629409575308685
1102	30-31	0.6881618180987559
1102	32-33	0.6747755708998397
1102	34-35	0.6514190640683566
1102	36-37	0.6984729624780428
1102	38-39	0.7063991990461957
1102	40-41	0.6876928307811028
1102	42-43	0.6812303527261676
1102	44-45	0.7157838585940439
1102	46-47	0.6236696240043216
1102	48-49	0.6150435443647879
1102	50-51	0.6277986275115097
1102	52-53	0.5697479320639189
1102	54-55	0.5673654386985447
1102	56-57	0.5831405260884921
1102	58-59	0.5428962445428382
1102	60-61	0.5497744947486041
1102	62-63	0.5644904020577215
1102	64-65	0.5098293060636436
1102	66-67	0.5682953053333861
1102	68-69	0.5118800856979888
1102	70-71	0.4951172718624477
1102	72-73	0.4804652816549009
1102	74-75	0.44454528051061715
1102	76-77	0.4612273278857728
1102	78-79	0.4558538601286415
1102	80-81	0.4431634335809562
1102	82-83	0.5094999034783356
1102	84-85	0.5178856087975063
1102	86-87	0.43010262604421
1102	88-89	0.4968283678776544
1102	90-91	0.48090412909425595
1102	92-93	0.4991577737845887
1102	94-95	0.49454178577961727
1102	96-97	0.46111966611990596
1102	98-99	0.46652025061946034
1102	100	0.4888623032219286
1103	1	0.3115973815432014
1103	2	0.34113948787477
1103	3	0.3553248053692819
1103	4	0.4212936663975242
1103	5	0.24942578951169736
1103	6	0.2792371521714827
1103	7	0.3127637292576608
1103	8	0.31573174931515524
1103	9	0.42606854733001853
1103	10-11	0.5469398846810947
1103	12-13	0.49874184898238383
1103	14-15	0.5907087244338598
1103	16-17	0.5443844548448169
1103	18-19	0.5606697987748603
1103	20-21	0.4714525127842535
1103	22-23	0.54209995799323
1103	24-25	0.5481107924424506
1103	26-27	0.4526471623551842
1103	28-29	0.4568638962341538
1103	30-31	0.5072561888782658
1103	32-33	0.4795994979134264
1103	34-35	0.5165022498674361
1103	36-37	0.5086526230831936
1103	38-39	0.4911467226120152
1103	40-41	0.4711424713955381
1103	42-43	0.46928593805385077
1103	44-45	0.3841013277747294
1103	46-47	0.39858031092902735
1103	48-49	0.46862516056577164
1103	50-51	0.3628720002934216
1103	52-53	0.32974427095282977
1103	54-55	0.3750277676212548
1103	56-57	0.3498044252051713
1103	58-59	0.3984144200764348
1103	60-61	0.4324434876403416
1103	62-63	0.3553968710773532
1103	64-65	0.3363618024368975
1103	66-67	0.403625329312554
1103	68-69	0.3777199646842888
1103	70-71	0.3193865850378401
1103	72-73	0.33223344815091593
1103	74-75	0.2522630345873935
1103	76-77	0.24322513296704074
1103	78-79	0.32482734988654727
1103	80-81	0.3320258537454208
1103	82-83	0.28804060429985867
1103	84-85	0.31873834259000233
1103	86-87	0.2876334751558218
1103	88-89	0.37485575041442587
1103	90-91	0.34117016506673536
1103	92-93	0.3040153165990489
1103	94-95	0.26902110832787685
1103	96-97	0.2974473428724025
1103	98-99	0.2822798163646496
1103	100	0.3842741635216207
1104	1	0.19164967550756629
1104	2	0.27707202763057026
1104	3	0.21003951124862397
1104	4	0.36848795458544714
1104	5	0.22523627816077152
1104	6	0.23781575374016484
1104	7	0.2615190915129517
1104	8	0.2885757783433789
1104	9	0.3571125298083828
1104	10-11	0.45790171668389235
1104	12-13	0.3662646850819229
1104	14-15	0.47897622885729874
1104	16-17	0.4456409193313391
1104	18-19	0.4482251232739429
1104	20-21	0.42048953394588295
1104	22-23	0.4611724246277191
1104	24-25	0.47450353596449446
1104	26-27	0.4487271707057374
1104	28-29	0.38117637125766635
1104	30-31	0.3727640029212367
1104	32-33	0.45087305881398265
1104	34-35	0.43847729496125254
1104	36-37	0.42647630803653414
1104	38-39	0.42036769111031447
1104	40-41	0.42018788592896783
1104	42-43	0.47090590535476196
1104	44-45	0.3859420826432469
1104	46-47	0.379815376816687
1104	48-49	0.37661472274857744
1104	50-51	0.31265890949428865
1104	52-53	0.36686841816840854
1104	54-55	0.42832500366375115
1104	56-57	0.4487657539780159
1104	58-59	0.43028084128636124
1104	60-61	0.42119111775397755
1104	62-63	0.38129409226932864
1104	64-65	0.38417312446556195
1104	66-67	0.37641557715160445
1104	68-69	0.38082877030561235
1104	70-71	0.32696464393858804
1104	72-73	0.36265646540145724
1104	74-75	0.37774675112030565
1104	76-77	0.31196369854941963
1104	78-79	0.31907335995530417
1104	80-81	0.29693756462766885
1104	82-83	0.32505057993859765
1104	84-85	0.4456109992529065
1104	86-87	0.4120950620752666
1104	88-89	0.3073733281673796
1104	90-91	0.2951695927660012
1104	92-93	0.3374262733655513
1104	94-95	0.330182305407007
1104	96-97	0.3773875845475203
1104	98-99	0.33375071831328285
1104	100	0.3673576096793525
1105	1	0.039000039512853135
1105	2	0.15650350137634916
1105	3	0.1557532979294578
1105	4	0.2056662629112509
1105	5	0.10919889256825854
1105	6	0.1255216940944237
1105	7	0.16987432997601815
1105	8	0.1555902806963303
1105	9	0.294378290391748
1105	10-11	0.31981506364661527
1105	12-13	0.1727128042769337
1105	14-15	0.33023524360744005
1105	16-17	0.2738384625851893
1105	18-19	0.25175670025446806
1105	20-21	0.2257144700787137
1105	22-23	0.27617550908254884
1105	24-25	0.27020265195645976
1105	26-27	0.2666351442091681
1105	28-29	0.2888310699499286
1105	30-31	0.30334571043159286
1105	32-33	0.2717563036125412
1105	34-35	0.3546257138990043
1105	36-37	0.30346041727157314
1105	38-39	0.3354974763882268
1105	40-41	0.3270296425177719
1105	42-43	0.3281456618865022
1105	44-45	0.31530817885376905
1105	46-47	0.3092195634645307
1105	48-49	0.22535603866399612
1105	50-51	0.2721787504987816
1105	52-53	0.29509517181173095
1105	54-55	0.2529386353215415
1105	56-57	0.23910627466031542
1105	58-59	0.33446608339761497
1105	60-61	0.3612007396428467
1105	62-63	0.33731043571066976
1105	64-65	0.3375680087720667
1105	66-67	0.3205757548126229
1105	68-69	0.32685859020597974
1105	70-71	0.28016366879783305
1105	72-73	0.2548260634849555
1105	74-75	0.24147555589652825
1105	76-77	0.2587525956110568
1105	78-79	0.3196261784767458
1105	80-81	0.21566090192057885
1105	82-83	0.2469811895609908
1105	84-85	0.1642900129826188
1105	86-87	0.20367498760171543
1105	88-89	0.18841087014713054
1105	90-91	0.1947592463776786
1105	92-93	0.23700780410560895
1105	94-95	0.2056998329665447
1105	96-97	0.1768365998621917
1105	98-99	0.20948634138162348
1105	100	0.26803865935052684
1106	1	0.09761924473875538
1106	2	0.217742131710569
1106	3	0.1995070843121418
1106	4	0.284748036032191
1106	5	0.15615430679852693
1106	6	0.216819083272199
1106	7	0.19422434356245333
1106	8	0.1798013983820539
1106	9	0.2760331580217894
1106	10-11	0.36180237412949623
1106	12-13	0.2617615105838027
1106	14-15	0.41297340491278334
1106	16-17	0.2907729817987388
1106	18-19	0.3777793019893778
1106	20-21	0.3050514837512637
1106	22-23	0.3128848372074984
1106	24-25	0.35728491402809226
1106	26-27	0.3538589780156727
1106	28-29	0.350031434308832
1106	30-31	0.3913417080023649
1106	32-33	0.3428897124136796
1106	34-35	0.3604553125102683
1106	36-37	0.30667491387075785
1106	38-39	0.3351439956012854
1106	40-41	0.29731522331670135
1106	42-43	0.29611042829639445
1106	44-45	0.30676013380274014
1106	46-47	0.29952425064744403
1106	48-49	0.227290914242964
1106	50-51	0.2640863396841837
1106	52-53	0.14287237183617663
1106	54-55	0.24951217553490324
1106	56-57	0.25393178026614294
1106	58-59	0.2421304473620438
1106	60-61	0.2557729043846422
1106	62-63	0.1971240637062337
1106	64-65	0.25693548148041856
1106	66-67	0.22818041519388998
1106	68-69	0.17728006347510927
1106	70-71	0.2356903227665086
1106	72-73	0.20585472528823345
1106	74-75	0.18947067245277083
1106	76-77	0.2230909029643584
1106	78-79	0.17249193345423208
1106	80-81	0.1633943203485302
1106	82-83	0.21897876287328444
1106	84-85	0.16460544433574853
1106	86-87	0.21858125545092122
1106	88-89	0.24036193529617123
1106	90-91	0.2517255362497721
1106	92-93	0.2829058075559274
1106	94-95	0.19942997456487888
1106	96-97	0.19869498648947825
1106	98-99	0.22560698939255985
1106	100	0.3426155187119022
1107	1	0.08441672535890987
1107	2	0.1956975580671596
1107	3	0.1574770455524508
1107	4	0.25531489649731043
1107	5	0.12332969439542296
1107	6	0.18956617629545747
1107	7	0.15425341332989717
1107	8	0.1726550805525946
1107	9	0.26440525104504786
1107	10-11	0.3090528586256198
1107	12-13	0.21331189818069873
1107	14-15	0.3447200134399182
1107	16-17	0.2432923616436966
1107	18-19	0.20741835237696904
1107	20-21	0.20348898375125657
1107	22-23	0.3438320271299773
1107	24-25	0.32585322798157534
1107	26-27	0.2664679896435871
1107	28-29	0.28323153120805955
1107	30-31	0.3580931614907428
1107	32-33	0.29867944109584954
1107	34-35	0.27833321948701695
1107	36-37	0.26858140611881964
1107	38-39	0.347377522733062
1107	40-41	0.2664891574252266
1107	42-43	0.3066482189940629
1107	44-45	0.2647300950430491
1107	46-47	0.29122725452341314
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2216	84-85	-0.9522176312338608
2216	86-87	-0.9153525626310994
2216	88-89	-0.9200793665882685
2216	90-91	-0.9513901505645244
2216	92-93	-0.8734411653325012
2216	94-95	-0.9328400455221342
2216	96-97	-0.9592385954480349
2216	98-99	-0.9686241813453904
2216	100	-0.6169559947681194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14.0
3	33.0
4	40.0
5	42.0
6	85.0
7	380.0
8	775.0
9	1174.0
10	1491.0
11	2191.0
12	3358.0
13	5008.0
14	7900.0
15	11571.0
16	14586.0
17	16978.0
18	19099.0
19	21203.0
20	23984.0
21	28013.0
22	32801.0
23	38122.0
24	44338.0
25	51592.0
26	57888.0
27	65422.0
28	76314.0
29	91530.0
30	108728.0
31	130240.0
32	163063.0
33	211906.0
34	311538.0
35	452775.0
36	730314.0
37	1281079.0
38	1020776.0
39	388761.0
40	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.166279145393645	20.879383819003667	11.333500610475342	24.620836425127344
2	15.817940934111089	20.708444954313116	39.957879200768545	23.51573491080725
3	18.168538377568204	28.68553391637662	29.00799476114241	24.13793294491277
4	11.704822387710191	17.187785096775006	41.51077374941549	29.596618766099308
5	12.504092674588005	39.55434028486307	35.42602503378014	12.51554200676877
6	28.111957509492203	39.85041825531791	19.062282269294784	12.975341965895096
7	25.50128043857699	32.41197400270196	25.313554182827573	16.773191375893482
8	25.88375311733362	34.53290085378701	20.71565947129283	18.867686557586534
9	26.996446203235813	14.48104318569396	21.739615370055592	36.782895241014636
10-11	25.3463008110752	25.692659328565092	28.1276207368232	20.833419123536505
12-13	25.939503907220907	24.096514025480865	27.6242032382716	22.33977882902663
14-15	23.23790797558134	25.045874083880186	25.272867009752066	26.44335093078641
16-17	22.16883679978491	27.18493367724551	26.65185502801673	23.99437449495285
18-19	21.36398579503853	28.503851408908588	28.003665915645158	22.12849688040772
20-21	23.22394018462402	27.395080262883333	26.595175178613307	22.78580437387934
22-23	23.8925564741446	27.131964744400598	26.19247097580748	22.783007805647323
24-25	23.379266675542734	27.096689368348375	27.186296410307737	22.337747545801157
26-27	23.10051920865185	27.438033271014504	26.31163155908113	23.14981596125251
28-29	22.278446393285034	28.261259174182108	26.522131469271688	22.938162963261167
30-31	23.051561473782243	27.777058077190457	26.55832746467006	22.61305298435724
32-33	23.509577975186158	27.999983010161678	25.516890438534507	22.97354857611766
34-35	23.48182974865055	27.446054325164308	26.877032484668074	22.19508344151707
36-37	23.225642924483438	27.130097388596848	26.607779822545364	23.03647986437435
38-39	23.43510070273354	27.09487189004906	27.17957184463814	22.290455562579258
40-41	22.79204049631663	26.315154299778893	27.85347065776967	23.03933454613481
42-43	22.837424050783117	26.722424398457083	26.872325900066958	23.567825650692843
44-45	22.704161519357346	27.934751280258997	26.873299679465422	22.487787520918232
46-47	22.849747947211313	27.3595387952959	26.438069482866062	23.35264377462672
48-49	23.61196419654682	26.550225930269466	26.91770604564448	22.920103827539233
50-51	23.126263364572168	26.63746223816049	26.579819245538	23.656455151729343
52-53	22.39051010626213	27.44638407674959	26.526837053031326	23.63626876395695
54-55	22.46140715715852	27.371078531008596	26.394528133622114	23.772986178210775
56-57	23.85487845791047	26.47638337888828	26.150057341149225	23.518680822052023
58-59	22.494335241240645	26.71022523008797	27.53998529660693	23.25545423206446
60-61	22.656749673144795	27.631893163457892	26.39335248815352	23.318004675243795
62-63	23.167449452573127	26.79929411219354	25.92741500217508	24.105841433058252
64-65	21.904762784195185	27.10786339331926	26.59582611730844	24.391547705177118
66-67	23.035909310727288	26.358750330241477	26.466902393593912	24.138437965437326
68-69	22.13640510734075	26.554908769852126	26.786281650915406	24.522404471891722
70-71	22.26822825554706	26.299211827727337	26.799029967388872	24.63352994933673
72-73	22.907176420084692	26.289366180724798	27.4961612719175	23.30729612727301
74-75	22.917106824202435	26.849021779117994	26.790671664393855	23.44319973228572
76-77	22.505464441264056	26.518960463863678	26.755239662992803	24.220335431879462
78-79	22.36766530986134	26.677744575856742	26.600907163488884	24.35368295079303
80-81	22.07154594214254	26.952729777659172	27.35969978424763	23.61602449595066
82-83	22.899652150403398	25.553436538316642	28.469428533860857	23.077482777419103
84-85	23.114024626555782	26.367342476297907	26.96925231004968	23.549380587096632
86-87	21.547876706389978	27.165716452250905	27.516706201606866	23.76970063975225
88-89	22.901932032815058	25.716854028034717	27.09247690512785	24.288737034022375
90-91	22.393002506452664	25.63476931779849	27.344934076222643	24.6272940995262
92-93	22.884007763065824	25.933101638063032	27.261295790795387	23.92159480807576
94-95	21.50158247542814	26.008405642617248	27.982381687385292	24.507630194569316
96-97	21.850758385753476	25.80497776150968	27.488818425937612	24.855445426799232
98-99	21.249112049086452	26.740330695866344	27.253354637691558	24.75720261735565
100	22.13100291801797	25.703385630302805	26.296894303912655	25.868717147766574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	879.0
1	660.0
2	564.0
3	682.5
4	635.0
5	536.5
6	459.0
7	447.0
8	480.0
9	464.0
10	373.5
11	332.5
12	331.0
13	317.0
14	355.5
15	463.0
16	519.5
17	540.0
18	674.0
19	822.5
20	972.0
21	1194.5
22	1606.0
23	2244.0
24	2902.5
25	3695.5
26	5495.5
27	7689.5
28	11311.0
29	21155.0
30	36122.0
31	50536.0
32	71597.5
33	91444.5
34	108828.5
35	128017.0
36	144989.5
37	175579.0
38	188075.0
39	174346.5
40	158237.5
41	142629.5
42	140067.0
43	150782.5
44	168474.5
45	201998.0
46	233234.0
47	257129.5
48	275828.0
49	266804.0
50	244657.0
51	243168.5
52	245129.0
53	236040.5
54	222898.5
55	193063.5
56	171387.0
57	139979.0
58	106492.0
59	101620.5
60	83540.0
61	56626.5
62	42114.5
63	34676.5
64	25300.5
65	12133.0
66	6237.0
67	4506.0
68	3501.0
69	2590.0
70	1911.5
71	1381.0
72	928.0
73	650.0
74	470.5
75	362.5
76	249.0
77	124.0
78	80.5
79	64.0
80	52.5
81	44.5
82	43.0
83	41.0
84	30.0
85	22.0
86	21.0
87	17.0
88	11.0
89	13.0
90	14.5
91	12.0
92	11.5
93	10.5
94	7.5
95	8.0
96	9.5
97	4.5
98	4.0
99	9.0
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05910945812267549
2	0.008771319152849377
3	0.008420466386735402
4	0.010137798347188016
5	0.003951710102546877
6	0.0026590946484427586
7	8.30967077638362E-4
8	0.0016988660253939847
9	7.755692724624711E-4
10-11	6.370747595227442E-4
12-13	3.5085276611397507E-4
14-15	1.8465935058630266E-4
16-17	7.571033374038409E-4
18-19	0.0015880704150422029
20-21	8.678989477556227E-4
22-23	0.0012372176489282279
24-25	0.0012187517138695977
26-27	0.003933244167488247
28-29	0.004588784862069621
30-31	0.005844468446056479
32-33	0.006933958614515665
34-35	0.003822448557136465
36-37	0.008715921347673487
38-39	0.007017055322279501
40-41	0.006758532231458677
42-43	0.015169765650664763
44-45	0.02106039893436782
46-47	0.019112242785682326
48-49	0.022279150648237416
50-51	0.018752157052039035
52-53	0.025981570627492786
54-55	0.019638521934853288
56-57	0.015760675572540933
58-59	0.018105849324986975
60-61	0.01661934155276724
62-63	0.011735101729759535
64-65	0.011716635794700904
66-67	0.014375730443143662
68-69	0.005696740965587437
70-71	0.00937146204225486
72-73	0.009186802691668558
74-75	0.011984391853051043
76-77	0.011014930262472954
78-79	0.012870756735865296
80-81	0.011162657742941997
82-83	0.007257112478041695
84-85	0.011310385223411039
86-87	0.010091633509541441
88-89	0.008946745535906365
90-91	0.013895616131619274
92-93	0.009343763139666915
94-95	0.008180409230973209
96-97	0.010220895054951853
98-99	0.009648451068134315
100	0.2605174118071558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5415377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.56646372530213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15646966598192	46.50298075293805
2	15.412145504156877	19.593911762381328
3	5.582262598497926	10.645340789175876
4	2.482687610433721	6.3126268771956875
5	1.2456762245712345	3.9591616271339345
6	0.7031590636759765	2.681840106856582
7	0.42747501041321456	1.9021152320033325
8	0.2696820084482377	1.371418528591322
9	0.18166934978498772	1.0393270319795982
>10	0.5283284593794221	4.861261903559339
>50	0.006031877775985182	0.2566589610259959
>100	0.004105835965157949	0.5075618754326953
>500	1.3148182115161242E-4	0.06903571220392854
>1k	1.7530909422015564E-4	0.296758839522419
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.232967529315133E-5
2	0.0	0.0	0.0	0.0	9.232967529315133E-5
3	0.0	0.0	0.0	0.0	9.232967529315133E-5
4	0.0	0.0	0.0	0.0	9.232967529315133E-5
5	0.0	0.0	0.0	0.0	9.232967529315133E-5
6	0.0	0.0	0.0	0.0	1.661934155276724E-4
7	0.0	0.0	0.0	0.0	1.661934155276724E-4
8	0.0	0.0	0.0	0.0	1.661934155276724E-4
9	0.0	0.0	0.0	0.0	1.661934155276724E-4
10-11	0.0	0.0	0.0	0.0	1.661934155276724E-4
12-13	0.0	0.0	0.0	0.0	3.323868310553448E-4
14-15	0.0	0.0	0.0	0.0	3.877846362312356E-4
16-17	0.0	0.0	0.0	0.0	4.801143115243869E-4
18-19	0.0	0.0	0.0	2.76989025879454E-5	5.078132141123323E-4
20-21	0.0	0.0	0.0	2.4005715576219346E-4	6.093758569347988E-4
22-23	0.0	0.0	0.0	6.093758569347988E-4	6.463077270520593E-4
24-25	0.0	0.0	0.0	0.0011541209411643917	6.463077270520593E-4
26-27	0.0	0.0	0.0	0.0021605144018597413	7.386374023452106E-4
28-29	0.0	0.0	0.0	0.007294044348158955	7.571033374038409E-4
30-31	0.0	0.0	0.0	0.02369179468022263	7.848022399917863E-4
32-33	0.0	0.0	0.0	0.05208316983286666	8.217341101090468E-4
34-35	0.0	0.0	0.0	0.0920065214296253	9.232967529315134E-4
36-37	0.0	0.0	0.0	0.1552800479080219	9.417626879901437E-4
38-39	0.0	0.0	0.0	0.2574705325224818	9.602286230487739E-4
40-41	0.0	0.0	0.0	0.3784131741889808	9.602286230487739E-4
42-43	0.0	0.0	0.0	0.5038984358799028	9.694615905780891E-4
44-45	0.0	0.0	0.0	0.6399739113269491	0.0010710242334005555
46-47	0.0	0.0	0.0	0.8055025531925109	0.0011448879736350765
48-49	0.0	0.0	0.0	0.9960248381599286	0.001181819843752337
50-51	0.0	0.0	0.0	1.213516621280476	0.0012372176489282279
52-53	0.0	0.0	0.0	1.4299189142325641	0.0013110813891627489
54-55	0.0	0.0	0.0	1.6466905258858247	0.0013572462268093247
56-57	0.0	0.0	0.0	1.9066262607386335	0.0014218769995145305
58-59	0.0	0.0	0.0	2.225726851519294	0.0014772748046904213
60-61	0.0	0.0	0.0	2.5747976549001113	0.0014772748046904213
62-63	0.0	0.0	0.0	2.9424082570797934	0.0016434682202180937
64-65	0.0	0.0	0.0	3.312474828622273	0.0016988660253939845
66-67	0.0	0.0	0.0	3.705817711306157	0.0017265649279819299
68-69	0.0	0.0	0.0	4.143903554637101	0.001735797895511245
70-71	0.0	0.0	0.0	4.640129394500143	0.0017542638305698753
72-73	0.0	0.0	0.0	5.176407847505354	0.0018281275708043965
74-75	0.0	9.232967529315133E-6	0.0	5.694211132484405	0.0019112242785682327
76-77	0.0	1.8465935058630267E-5	0.0	6.261724714641289	0.0020127869213906993
78-79	0.0	1.8465935058630267E-5	0.0	6.868201419771883	0.0020404858239786447
80-81	0.0	1.8465935058630267E-5	0.0	7.538007049186049	0.0020589517590372747
82-83	0.0	1.8465935058630267E-5	0.0	8.268796429131342	0.002225145174564947
84-85	0.0	1.8465935058630267E-5	0.0	8.975016882481128	0.002234378142094262
86-87	0.0	1.8465935058630267E-5	0.0	9.700044890688128	0.002280542979740838
88	0.0	1.8465935058630267E-5	0.0	10.259876643860622	0.0023267078173874137
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5110	0.0	37.812122	94
GTACTAG	6145	0.0	30.529163	1
TAGTACT	6900	0.0	29.695644	4
CTGTGCG	6980	0.0	29.48777	9
TAGGCAT	7255	0.0	28.889282	5
TATTCTC	3015	0.0	28.835247	5
TACCCCG	375	0.0	28.822779	5
CTAACGC	3265	0.0	28.499504	3
CGCCTAC	3290	0.0	28.423952	7
ACTGTGC	7510	0.0	28.220446	8
TCTAACG	3365	0.0	28.072063	2
TAACGCC	3395	0.0	27.685064	4
GTACATG	24620	0.0	27.557652	1
GTACTGT	7750	0.0	27.22525	6
ACATGGG	24295	0.0	27.119797	3
TACATGG	24830	0.0	27.084837	2
GCATCGT	6155	0.0	26.721895	8
CTAGGCA	8485	0.0	25.97624	4
CATGGGG	19670	0.0	25.731592	4
AGGGCTA	6620	0.0	25.69744	5
>>END_MODULE