FastQCFastQC Report
Mon 16 Jan 2017
ERR1146661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5415377
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123410.22788810455855613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84460.15596328750519123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64460.1190314173879307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC54350.041.7902994
GTATCAA228900.033.109611
GTACTAG56800.032.052871
CTGTGCG75100.031.6078329
TAGTACT75700.031.3195084
ACTGTGC80250.030.0432858
TATTCTC28350.030.0063135
TAGGCAT72900.029.0760975
GTACTGT83250.028.8474716
AGGGCTA67350.028.7505875
CAACGCA255300.028.4238935
CTAACGC28150.028.0744423
ATCAACG260900.027.838993
TACTGTG93300.027.7048727
CGCCTAC29100.027.4556037
TATCAAC279700.027.0288622
TCTAACG29550.026.7458342
TAACGCC30100.026.7245184
CTAGTAC94000.026.723523
GCTATTG71850.026.6875348
Produced by FastQC (version 0.11.5)