FastQCFastQC Report
Mon 16 Jan 2017
ERR1146660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5857673
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA83430.14242857189194413No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC69100.042.89079794
GTACTAG78000.037.254871
GCATCGT79550.034.0243388
TAGGCAT92100.033.7781145
CTGTGCG94550.033.5466849
TAGTACT97400.033.4391944
ACTGTGC102950.031.6310988
CAACGCA98100.031.376855
CTAGGCA105300.030.7965624
GTACTGT104600.030.7735356
TACTGTG113400.030.3325637
TATTCTC30500.029.8908965
GTATCAA118450.029.2564531
ATCAACG105850.028.9937133
CGCCTAC35400.028.937727
TCTAACG36000.028.8521842
CTAGTAC119150.028.7945543
AGGGCTA70000.028.598855
CTAACGC36000.028.590593
TACCCCG3800.028.4433845
Produced by FastQC (version 0.11.5)