FastQCFastQC Report
Mon 16 Jan 2017
ERR1146660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5857673
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC167220.2854717223033788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113110.19309715649883494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96490.1647241148490194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59870.10220782211639332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC68400.043.2412994
GGTATCA113800.040.1004071
GTATCAA193100.038.3479161
GTACTAG76950.034.4206471
CTGTGCG95200.032.6865739
CAACGCA219000.032.3851055
TAGGCAT91750.032.2214245
ATCAACG221700.031.9979043
TAGTACT99650.031.581644
GTGGTAT27950.031.1393241
ACTGTGC102400.030.7505428
TATCAAC247200.029.9744592
GTACTGT107000.029.4719736
TACCCCG4150.029.445945
CTAGGCA106150.028.9830194
TACTGTG115750.028.9493647
AACGCAG262550.028.1749526
CTAGTAC118950.027.6834013
GCATCGT90750.027.4995618
TATTCTC34950.026.7612275
Produced by FastQC (version 0.11.5)