FastQCFastQC Report
Mon 16 Jan 2017
ERR1146659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096924
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC44000.041.97980594
GTACTAG49950.033.792141
GCATCGT47600.032.1847468
TAGGCAT55100.031.8988695
CTGTGCG62050.031.4293279
TAGTACT62350.031.1296444
CAACGCA68850.031.1254885
CCGTGTT5150.031.024199
TGAACCG5050.030.7098625
ACTGTGC67100.029.5548888
TATTCTC21600.029.1545495
GTATCAA84250.029.0753251
CGCCTAC22800.029.0618767
CTAACGC23000.028.8105723
TACTGTG76400.028.7251917
TCTAACG23400.028.7197572
GTACTGT69250.028.705166
ATCAACG75600.028.4711023
TAACGCC23450.028.458114
TACCCCG2151.8189894E-1228.4158175
Produced by FastQC (version 0.11.5)