##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146659_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4096924
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459951173124033	31.0	31.0	34.0	28.0	34.0
2	31.466779710826952	31.0	31.0	34.0	28.0	34.0
3	31.631754945905758	33.0	31.0	34.0	28.0	34.0
4	35.08310405562808	37.0	35.0	37.0	32.0	37.0
5	35.21275547215423	37.0	35.0	37.0	32.0	37.0
6	35.30005413817781	37.0	35.0	37.0	32.0	37.0
7	35.19382102279661	37.0	35.0	37.0	32.0	37.0
8	35.175786516908786	37.0	35.0	37.0	32.0	37.0
9	36.7013564322892	39.0	37.0	39.0	32.0	39.0
10-11	36.56501109124797	39.0	37.0	39.0	32.0	39.0
12-13	36.51080090819356	39.0	37.0	39.0	32.0	39.0
14-15	37.69998259669938	40.0	38.0	41.0	32.0	41.0
16-17	37.726593048833706	40.0	38.0	41.0	32.0	41.0
18-19	37.68989795758964	40.0	37.0	41.0	31.5	41.0
20-21	37.763505498271385	40.0	37.5	41.0	32.0	41.0
22-23	37.697378691916185	40.0	37.0	41.0	31.5	41.0
24-25	37.656749429572045	40.0	37.0	41.0	31.0	41.0
26-27	37.69671062973099	40.0	37.0	41.0	31.0	41.0
28-29	37.68262347556362	40.0	37.0	41.0	31.0	41.0
30-31	37.548373975206765	40.0	37.0	41.0	31.0	41.0
32-33	37.5414481694071	40.0	37.0	41.0	31.0	41.0
34-35	37.47458800797867	40.0	37.0	41.0	31.0	41.0
36-37	37.355814630683895	40.0	36.0	41.0	30.0	41.0
38-39	37.25733721201565	40.0	36.0	41.0	30.0	41.0
40-41	37.175114915482936	40.0	36.0	41.0	30.0	41.0
42-43	37.00549021656248	39.5	35.5	41.0	30.0	41.0
44-45	36.84054671260682	39.0	35.0	41.0	30.0	41.0
46-47	36.84166926210982	39.0	35.0	41.0	30.0	41.0
48-49	36.698553841857944	39.0	35.0	41.0	30.0	41.0
50-51	36.50878891089998	39.0	35.0	41.0	29.5	41.0
52-53	36.290721160558505	39.0	35.0	40.5	29.0	41.0
54-55	36.07670828162787	38.0	35.0	40.0	29.0	41.0
56-57	35.82708209866719	38.0	34.0	40.0	28.5	41.0
58-59	35.51959030726467	37.0	34.0	40.0	28.0	41.0
60-61	35.265020293273686	37.0	34.0	40.0	28.0	41.0
62-63	34.94972435417401	36.0	34.0	40.0	27.5	41.0
64-65	34.79475369814036	36.0	34.0	39.0	28.0	41.0
66-67	34.5390302578227	35.0	33.0	39.0	27.0	41.0
68-69	34.41388344035671	35.0	33.0	39.0	27.0	41.0
70-71	34.336701876822715	35.0	33.0	38.5	27.5	41.0
72-73	34.113896669794215	35.0	33.0	37.5	27.0	40.0
74-75	33.85827659971237	35.0	33.0	37.0	27.0	40.0
76-77	33.59276032457522	35.0	33.0	36.5	26.5	39.0
78-79	33.33618905305541	35.0	33.0	36.0	26.0	39.0
80-81	33.14611718450233	35.0	33.0	36.0	26.0	38.5
82-83	32.87816517953469	35.0	33.0	35.5	26.0	37.0
84-85	32.69314502783064	35.0	33.0	35.0	26.0	37.0
86-87	32.51387333521442	35.0	33.0	35.0	25.5	36.5
88-89	32.38700229733332	35.0	33.0	35.0	25.0	36.0
90-91	32.22241161905859	35.0	32.0	35.0	25.0	36.0
92-93	32.058009252795514	35.0	32.0	35.0	25.0	36.0
94-95	31.97825270861749	35.0	32.0	35.0	25.0	35.5
96-97	31.86524475435717	35.0	32.0	35.0	24.5	35.0
98-99	31.7601300390244	35.0	32.0	35.0	24.0	35.0
100	30.773588184696617	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46968523340190416
1101	2	0.5406359164030299
1101	3	0.5082106619600886
1101	4	0.603400461658886
1101	5	0.47897234109630205
1101	6	0.4776498092512895
1101	7	0.4993179277478319
1101	8	0.5099965811220244
1101	9	0.7347479288288952
1101	10-11	0.7761730591671991
1101	12-13	0.6250869751247805
1101	14-15	0.8959067724432614
1101	16-17	0.8225292350612108
1101	18-19	0.859667555434207
1101	20-21	0.8103295931927974
1101	22-23	0.8201034966959213
1101	24-25	0.8608085341139713
1101	26-27	0.8187193175676342
1101	28-29	0.8218263724023771
1101	30-31	0.8549071208310366
1101	32-33	0.814112781924031
1101	34-35	0.8347619431678837
1101	36-37	0.848197731209666
1101	38-39	0.822115787810823
1101	40-41	0.8206866289128953
1101	42-43	0.8106981406135745
1101	44-45	0.8399685156212016
1101	46-47	0.7736745875639173
1101	48-49	0.7447284140156327
1101	50-51	0.7584934875807647
1101	52-53	0.7350815904734844
1101	54-55	0.7228161408531548
1101	56-57	0.7479697204492552
1101	58-59	0.694857675774017
1101	60-61	0.7717242087196681
1101	62-63	0.7240793745763625
1101	64-65	0.7910959504562527
1101	66-67	0.7951264274522956
1101	68-69	0.7777662848183766
1101	70-71	0.7814886912289865
1101	72-73	0.7873220292995526
1101	74-75	0.7750404041660701
1101	76-77	0.7000870548644329
1101	78-79	0.7153290621532591
1101	80-81	0.7104785127884838
1101	82-83	0.6904721808186522
1101	84-85	0.6802710480129406
1101	86-87	0.692230120446574
1101	88-89	0.651685358662391
1101	90-91	0.6743561426132345
1101	92-93	0.6643084897883043
1101	94-95	0.6331115408035188
1101	96-97	0.5771153076142355
1101	98-99	0.6623508139998933
1101	100	0.7499938752714606
1102	1	0.41879981492713725
1102	2	0.516877614934895
1102	3	0.5295563320711487
1102	4	0.5776548837721407
1102	5	0.5055551575811066
1102	6	0.47666493982104186
1102	7	0.4971056505443201
1102	8	0.5464137631158223
1102	9	0.6483076285161928
1102	10-11	0.7239920051793192
1102	12-13	0.6858544894815779
1102	14-15	0.8824757829418743
1102	16-17	0.860735718231723
1102	18-19	0.8296546526045816
1102	20-21	0.8047713214057453
1102	22-23	0.8464476198228539
1102	24-25	0.8920758149363337
1102	26-27	0.8174405695611853
1102	28-29	0.8354555999324234
1102	30-31	0.8762130409878495
1102	32-33	0.8326655820759541
1102	34-35	0.8593079271846378
1102	36-37	0.8440303482185811
1102	38-39	0.8765475688535744
1102	40-41	0.7670276483899627
1102	42-43	0.8393506517415545
1102	44-45	0.7872250979798778
1102	46-47	0.7474516506231907
1102	48-49	0.7705393566496994
1102	50-51	0.7787495469938079
1102	52-53	0.6427354483144327
1102	54-55	0.6455240145113734
1102	56-57	0.6512672931156729
1102	58-59	0.6830366562360979
1102	60-61	0.6609512657417582
1102	62-63	0.68397705094673
1102	64-65	0.7010916255395188
1102	66-67	0.7634926420934676
1102	68-69	0.6544604053243575
1102	70-71	0.5841051759527147
1102	72-73	0.5762193442689778
1102	74-75	0.5929528898148035
1102	76-77	0.6090083608814041
1102	78-79	0.5707064190911098
1102	80-81	0.6368154853495298
1102	82-83	0.5903300823391788
1102	84-85	0.6414378875886797
1102	86-87	0.6280334187522811
1102	88-89	0.660702291396035
1102	90-91	0.6281139171247716
1102	92-93	0.6596083688925702
1102	94-95	0.6895891858801306
1102	96-97	0.6125196264730448
1102	98-99	0.6015702017980402
1102	100	0.6845554370103741
1103	1	0.3114998634304911
1103	2	0.3485000636388307
1103	3	0.38093160508068635
1103	4	0.4442574675497042
1103	5	0.3005905481959985
1103	6	0.3319929171063194
1103	7	0.3356115572156142
1103	8	0.3615888102650757
1103	9	0.47359322883037436
1103	10-11	0.5645947418109998
1103	12-13	0.5139188174440719
1103	14-15	0.6303985212948575
1103	16-17	0.6288402498132797
1103	18-19	0.6622007419894871
1103	20-21	0.544515495743056
1103	22-23	0.6216184098627977
1103	24-25	0.6655854092471287
1103	26-27	0.5610882389227996
1103	28-29	0.5085296957616308
1103	30-31	0.5894285825319301
1103	32-33	0.5416355258796628
1103	34-35	0.6385146496163614
1103	36-37	0.6063156584357401
1103	38-39	0.6979078048733669
1103	40-41	0.5882104596052216
1103	42-43	0.6513639943040559
1103	44-45	0.6059320254614633
1103	46-47	0.5157131359606666
1103	48-49	0.5191729888178074
1103	50-51	0.5553675234252182
1103	52-53	0.5022863938718984
1103	54-55	0.5977310935574494
1103	56-57	0.6253576108934098
1103	58-59	0.6289029365773331
1103	60-61	0.6066844028172866
1103	62-63	0.5039560477594662
1103	64-65	0.4985278167516043
1103	66-67	0.5683987976753357
1103	68-69	0.5299054639756804
1103	70-71	0.5441765992783871
1103	72-73	0.5279388241142939
1103	74-75	0.4787682586038713
1103	76-77	0.4131219692281647
1103	78-79	0.4341424791690187
1103	80-81	0.45062248815860073
1103	82-83	0.5301833858274989
1103	84-85	0.5160531492984077
1103	86-87	0.4626110425115115
1103	88-89	0.5354083289028537
1103	90-91	0.5548802611461667
1103	92-93	0.5054762918046904
1103	94-95	0.4388949960605615
1103	96-97	0.46750110275329604
1103	98-99	0.5392080165488977
1103	100	0.6530474524190453
1104	1	0.22687556065108083
1104	2	0.3279179015602516
1104	3	0.2563981835309548
1104	4	0.40123739809913417
1104	5	0.3158440131520095
1104	6	0.27498339738084354
1104	7	0.2816723013999294
1104	8	0.31866791812429796
1104	9	0.3326521131425153
1104	10-11	0.50824471548232
1104	12-13	0.39916643731428536
1104	14-15	0.5266900861839687
1104	16-17	0.5410105543701533
1104	18-19	0.4826558703413042
1104	20-21	0.5144819000576533
1104	22-23	0.5257840552818251
1104	24-25	0.5348671885446734
1104	26-27	0.49766527520902315
1104	28-29	0.46146240373207803
1104	30-31	0.3867865614311583
1104	32-33	0.4460402259159224
1104	34-35	0.4490290713966374
1104	36-37	0.46123372472422375
1104	38-39	0.4747913569818607
1104	40-41	0.4951144728207453
1104	42-43	0.5157983999154396
1104	44-45	0.4470064974626595
1104	46-47	0.45418762280274194
1104	48-49	0.407145140866227
1104	50-51	0.3060765195003228
1104	52-53	0.40009183465603826
1104	54-55	0.41805447931049144
1104	56-57	0.393966497672416
1104	58-59	0.4117567563918243
1104	60-61	0.47134143490256264
1104	62-63	0.47058003917133107
1104	64-65	0.407591813364391
1104	66-67	0.3603725782527505
1104	68-69	0.35992962868991185
1104	70-71	0.36159730934718937
1104	72-73	0.32757401880564885
1104	74-75	0.3025282082453842
1104	76-77	0.29115434060684464
1104	78-79	0.2444626467519626
1104	80-81	0.27470676797300086
1104	82-83	0.3216094690367015
1104	84-85	0.3386787369649724
1104	86-87	0.35581696029381504
1104	88-89	0.3092245932144806
1104	90-91	0.32225588641095726
1104	92-93	0.3523717496875989
1104	94-95	0.4151711962706983
1104	96-97	0.45727312853516366
1104	98-99	0.34190511557951453
1104	100	0.5379724351939643
1105	1	0.17160266118577283
1105	2	0.2519407963003424
1105	3	0.2641833981678836
1105	4	0.358719354706281
1105	5	0.31051591056921524
1105	6	0.26346377564001244
1105	7	0.3254120713083921
1105	8	0.347879981511241
1105	9	0.46601568785497705
1105	10-11	0.5026708386912375
1105	12-13	0.36567213163997536
1105	14-15	0.5292020863412219
1105	16-17	0.5198403163226146
1105	18-19	0.45795474967139427
1105	20-21	0.453345890211061
1105	22-23	0.47381195194602554
1105	24-25	0.5273822582019108
1105	26-27	0.5294771647584398
1105	28-29	0.5080407428906497
1105	30-31	0.5237618388517049
1105	32-33	0.5112623627062476
1105	34-35	0.5404079220377369
1105	36-37	0.5279160287040199
1105	38-39	0.5234700103231731
1105	40-41	0.4762036564118688
1105	42-43	0.4892320791849514
1105	44-45	0.5655621414742455
1105	46-47	0.5852499797704667
1105	48-49	0.47409247273234456
1105	50-51	0.4841705591009031
1105	52-53	0.5270241642780036
1105	54-55	0.4931306308833072
1105	56-57	0.48000274246933117
1105	58-59	0.4508360147623165
1105	60-61	0.5037466477098818
1105	62-63	0.5063525161393656
1105	64-65	0.5129888536910201
1105	66-67	0.44804134089900316
1105	68-69	0.32614829452593597
1105	70-71	0.4850164349529962
1105	72-73	0.3894698307022537
1105	74-75	0.38304537615031364
1105	76-77	0.36881954485082247
1105	78-79	0.4315086259967984
1105	80-81	0.28024654760911005
1105	82-83	0.34572422196589514
1105	84-85	0.3097008804588057
1105	86-87	0.3085183596884278
1105	88-89	0.3416723077600565
1105	90-91	0.345379679748433
1105	92-93	0.3011143761463302
1105	94-95	0.2678645312975405
1105	96-97	0.2936649993500424
1105	98-99	0.24847650426112722
1105	100	0.35256523628406455
1106	1	0.15672390774169642
1106	2	0.20676914896803922
1106	3	0.22169479678457904
1106	4	0.354282854495068
1106	5	0.18143904696025714
1106	6	0.28125565709925127
1106	7	0.31321272634389175
1106	8	0.2571509695885865
1106	9	0.39176504293164527
1106	10-11	0.45251816197448846
1106	12-13	0.32534207736407694
1106	14-15	0.4687314850966331
1106	16-17	0.37965243860172393
1106	18-19	0.4008045032692209
1106	20-21	0.3766802565857077
1106	22-23	0.39903785092234045
1106	24-25	0.40879529867530096
1106	26-27	0.4274379993976183
1106	28-29	0.39922395349577044
1106	30-31	0.45728036787847515
1106	32-33	0.419279587821471
1106	34-35	0.4405554820475004
1106	36-37	0.4131835742802892
1106	38-39	0.437964792494526
1106	40-41	0.4158675930364879
1106	42-43	0.4139231246567192
1106	44-45	0.40153375563282623
1106	46-47	0.4316429923372027
1106	48-49	0.36562243117305115
1106	50-51	0.405857176074889
1106	52-53	0.3963922339893742
1106	54-55	0.3761753673922996
1106	56-57	0.3673987411559594
1106	58-59	0.4030020793065461
1106	60-61	0.38853608430466835
1106	62-63	0.4360315991866699
1106	64-65	0.3155253096221031
1106	66-67	0.2801660401409052
1106	68-69	0.35022109144408375
1106	70-71	0.3479245529602579
1106	72-73	0.34953167738242996
1106	74-75	0.2962927730559457
1106	76-77	0.2179361975253542
1106	78-79	0.26033334022507404
1106	80-81	0.2586332620187761
1106	82-83	0.2848120804324452
1106	84-85	0.2801430948919972
1106	86-87	0.282610968690058
1106	88-89	0.3593119974984589
1106	90-91	0.3044946203873593
1106	92-93	0.38720318956985267
1106	94-95	0.33385455590138946
1106	96-97	0.3227292530649919
1106	98-99	0.2851372976271591
1106	100	0.2632280171633923
1107	1	0.26911409766568184
1107	2	0.26851164718895504
1107	3	0.2641249254975584
1107	4	0.30828390730899
1107	5	0.24957626210906625
1107	6	0.2958111215346264
1107	7	0.24037830076809286
1107	8	0.22456705038557345
1107	9	0.3037697609200336
1107	10-11	0.4234906088647321
1107	12-13	0.3394153539811029
1107	14-15	0.4498300681165759
1107	16-17	0.34413030959507296
1107	18-19	0.3598229294323687
1107	20-21	0.35652558795097633
1107	22-23	0.4860913107405054
1107	24-25	0.5367929272047363
1107	26-27	0.4309693647590862
1107	28-29	0.4741555853717472
1107	30-31	0.5482117962936073
1107	32-33	0.4855719073183522
1107	34-35	0.5138163356521375
1107	36-37	0.5630987109517562
1107	38-39	0.5464434715729425
1107	40-41	0.45980837057364
1107	42-43	0.5199269472254144
1107	44-45	0.5325082467287743
1107	46-47	0.4788260192876095
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2215	26-27	-1.043561669327147
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2215	100	-0.41775527306028337
2216	1	-0.695424412326048
2216	2	-0.8896905139048705
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2216	4	-0.6891156371521561
2216	5	-0.6455320387560164
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2216	12-13	-1.0011115391446666
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2216	38-39	-1.5250649772699845
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2216	64-65	-1.3691667076305691
2216	66-67	-1.3294056056494483
2216	68-69	-1.3472430605279655
2216	70-71	-1.3880726524691909
2216	72-73	-1.2768090108301067
2216	74-75	-1.3107414539084274
2216	76-77	-1.2931771019625202
2216	78-79	-1.1764172441117964
2216	80-81	-1.193901619364219
2216	82-83	-1.1480380432014456
2216	84-85	-1.271038773555361
2216	86-87	-1.2008989142879116
2216	88-89	-1.2496736922897043
2216	90-91	-1.3128441384018608
2216	92-93	-1.2683329068858455
2216	94-95	-1.3035809447143656
2216	96-97	-1.3638445560767636
2216	98-99	-1.330412588018742
2216	100	-0.9708668068395632
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	9.0
3	22.0
4	33.0
5	25.0
6	33.0
7	158.0
8	406.0
9	667.0
10	962.0
11	1653.0
12	2807.0
13	4244.0
14	6173.0
15	8558.0
16	10531.0
17	12935.0
18	14567.0
19	16572.0
20	19200.0
21	21926.0
22	25498.0
23	29853.0
24	35694.0
25	41354.0
26	46377.0
27	51722.0
28	59456.0
29	71710.0
30	84308.0
31	98892.0
32	122504.0
33	161250.0
34	234791.0
35	334547.0
36	545013.0
37	935634.0
38	796064.0
39	300304.0
40	471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24388415819362	20.767938089015114	11.496163411160849	24.492014341630412
2	15.887512144157867	20.059121910257723	39.80518085641334	24.248185089171074
3	18.588169587007407	26.805498496196	29.032461222215144	25.573870694581448
4	11.91272272078501	16.43982147918018	40.81748728748292	30.829968512551893
5	12.765906550670072	38.959579787770274	35.542218816843544	12.732294844716115
6	29.519041598117564	39.05394916056844	18.26145644670745	13.16555279460655
7	26.667768317466795	31.9060690544663	24.093639992560195	17.33252263550671
8	26.402690451392154	34.95307014471331	20.054925004741435	18.589314399153107
9	26.46016571562192	14.480321452962706	21.440660714521083	37.618852116894296
10-11	25.722441696841887	25.769831128620265	27.880900338182112	20.626826836355736
12-13	25.584156076481456	23.809747555141804	27.856951859747138	22.749144508629605
14-15	23.04616734221447	25.03081581733799	25.027874584871277	26.89514225557626
16-17	22.018245726743206	27.500128450638766	26.511125655967817	23.97050016665021
18-19	21.315658339926795	28.55085533157516	27.94334150666309	22.19014482183496
20-21	23.079469099472576	27.588306050836636	26.926608684445362	22.405616165245426
22-23	23.603687935126125	27.322073117969865	26.496724025460882	22.577514921443125
24-25	23.04284458625436	27.26498745263771	27.547007147151355	22.145160813956583
26-27	22.973386778565942	27.453515621233706	26.38866700050613	23.184430599694224
28-29	22.05792809253869	28.12843297915434	26.863650382504282	22.949988545802693
30-31	22.94097989946069	27.739774345535167	26.925583627431404	22.39366212757274
32-33	23.414964873824044	27.832453755010178	25.915575127054723	22.837006244111056
34-35	23.231271407832132	27.597923001676318	27.06984941212941	22.10095617836214
36-37	22.875155754599223	27.17116937666549	26.729094220715606	23.22458064801969
38-39	23.122814075062053	27.274826541791075	27.346494611225136	22.25586477192174
40-41	22.668321184417948	26.35067281606342	28.04839647498378	22.932609524534854
42-43	22.63588267345779	26.684154686831718	27.23668612474392	23.443276514966573
44-45	22.687524756826168	27.712594381108335	27.175973198847437	22.423907663218053
46-47	22.74721657024914	27.389209968701177	26.640374565400943	23.223198895648743
48-49	23.51832429288986	26.94888328672732	26.8917427035589	22.641049716823915
50-51	22.994652080039607	26.672647047047832	26.941520033826706	23.39118083908586
52-53	22.341752365201433	27.472226179432912	26.78054644176363	23.40547501360202
54-55	22.425249209988714	27.323807463800097	26.72630649703721	23.524636829173975
56-57	23.65160756874292	26.659252648948943	26.42983799138465	23.259301790923477
58-59	22.375656551230833	27.161623596417666	27.51531596852216	22.947403883829338
60-61	22.429001454618337	27.86784834520533	26.652557873779358	23.05059232639697
62-63	22.921251930937377	27.03621130069504	26.174100246652227	23.868436521715356
64-65	21.949874208278672	27.372857110076176	26.754432003874573	23.92283667777058
66-67	22.70928555229493	26.82117483527421	26.691864690372142	23.77767492205872
68-69	22.071413498285995	26.952402189035276	26.997902331162187	23.978281981516545
70-71	22.10035880003559	26.550323850078044	27.090531679967018	24.258785669919348
72-73	22.74073743173861	26.55330594944208	27.636895655852495	23.069060962966816
74-75	22.904873418354978	26.84605708312594	27.082127511963417	23.166941986555667
76-77	22.564928347918762	26.83300229842745	26.813436798981765	23.788632554672024
78-79	22.41132501061903	26.768776150882967	26.747855444510083	24.072043393987922
80-81	21.942292770991546	27.157344637974695	27.47951080541298	23.420851785620783
82-83	22.78360136081688	26.0456879841295	28.59959962476722	22.571111030286403
84-85	23.08072184614626	26.594748623112814	27.158258387681975	23.166271143058953
86-87	21.56953304753864	27.355661089304718	27.690175869555954	23.384629993600683
88-89	22.86368245527613	26.014040485458366	27.365850360129496	23.75642669913601
90-91	22.469721414801423	26.019846317856	27.52953402482242	23.98089824252016
92-93	22.847163068673016	26.203621057688448	27.45520607445432	23.49400979918422
94-95	21.549859896319127	26.326737552937075	28.15432226995547	23.96908028078833
96-97	21.903433831296947	26.22494594493982	27.671198768222034	24.200421455541203
98-99	21.248652062870168	26.91429042209427	27.464328106358117	24.37272940867745
100	22.196986122891673	26.121271625319544	26.51886866792388	25.162873583864908
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	278.0
2	201.0
3	217.5
4	182.5
5	161.5
6	144.0
7	152.5
8	161.5
9	162.5
10	172.0
11	165.5
12	167.5
13	180.5
14	186.5
15	295.5
16	388.0
17	438.5
18	589.0
19	761.5
20	965.0
21	1261.5
22	1879.5
23	2769.5
24	3870.0
25	5242.5
26	7350.5
27	9682.5
28	13325.5
29	21733.5
30	34150.5
31	46156.0
32	61129.5
33	75144.5
34	85461.0
35	97910.0
36	109351.5
37	129096.5
38	136951.0
39	127601.5
40	118423.0
41	107890.5
42	106581.0
43	114684.0
44	126672.0
45	150740.0
46	173167.0
47	188416.5
48	198679.0
49	194369.5
50	183359.5
51	187101.0
52	190164.0
53	179904.0
54	168871.0
55	144304.5
56	125821.0
57	103547.0
58	79239.5
59	76434.5
60	62851.0
61	41349.5
62	30136.0
63	25426.5
64	18674.0
65	8398.0
66	4126.0
67	2818.5
68	2140.0
69	1585.5
70	1184.0
71	936.5
72	731.5
73	576.0
74	436.5
75	321.0
76	204.0
77	106.0
78	70.5
79	50.0
80	30.0
81	26.0
82	27.5
83	19.0
84	11.0
85	7.5
86	7.0
87	8.0
88	7.0
89	5.5
90	4.5
91	4.5
92	4.5
93	6.5
94	6.0
95	4.0
96	4.5
97	4.5
98	2.5
99	1.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05543183129586979
2	0.007420201106976844
3	0.007639878113433396
4	0.009055574377264505
5	0.0033439722093941697
6	0.0025384898523868153
7	0.001025159363463906
8	0.0015865561577417596
9	6.346224630967038E-4
10-11	4.637625691860527E-4
12-13	2.3188128459302636E-4
14-15	4.88171125459003E-5
16-17	4.759668473225278E-4
18-19	0.0015255347670593842
20-21	8.787080258262053E-4
22-23	0.0012570406480569326
24-25	0.0011472021448286568
26-27	0.0035270363814412964
28-29	0.0042104759570839
30-31	0.0053210652675031314
32-33	0.006443858856058838
34-35	0.003575853493987196
36-37	0.008189070629574774
38-39	0.006346224630967038
40-41	0.006395041743512938
42-43	0.014547499538678286
44-45	0.020295714540958045
46-47	0.018635932714397434
48-49	0.021625980857833828
50-51	0.01835523431725851
52-53	0.02503097445791037
54-55	0.019038673892901113
56-57	0.015365186173822116
58-59	0.017305666397521652
60-61	0.01612185141828357
62-63	0.011423204335740669
64-65	0.011472021448286568
66-67	0.013937285631854533
68-69	0.005699397889733859
70-71	0.008604016086214927
72-73	0.008665037476897302
74-75	0.01164288134219722
76-77	0.010434657806686187
78-79	0.012301912361566874
80-81	0.010629926256869789
82-83	0.006517084524877688
84-85	0.01111809738232879
86-87	0.009604766893405881
88-89	0.00834772624534895
90-91	0.01335148028130373
92-93	0.008750467423852626
94-95	0.007688695225979296
96-97	0.009922078124954234
98-99	0.00943390699949523
100	0.2593653189563683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4096924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.38455965424752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32152111734224	50.75507532980213
2	14.704639175069275	19.81731271373281
3	5.150519204029737	10.411964056628678
4	2.1514802728167868	5.799062031542381
5	1.0888690088593964	3.6686479341573692
6	0.5732557690597038	2.3177152540407087
7	0.32533300271246696	1.5345694790141593
8	0.1996747455969694	1.0763995844900545
9	0.1354736483365176	0.8215948924119559
>10	0.3431735448139575	3.2561380688659485
>50	0.003682007673100866	0.16760640009522673
>100	0.0022246469805797153	0.2192647200336823
>500	1.025711395248421E-4	0.046792283944391014
>1k	5.128556975831364E-5	0.10785725124051998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4408556272950145E-5
2	0.0	0.0	0.0	0.0	2.4408556272950145E-5
3	0.0	0.0	0.0	0.0	2.4408556272950145E-5
4	0.0	0.0	0.0	0.0	2.4408556272950145E-5
5	0.0	0.0	0.0	0.0	4.881711254590029E-5
6	0.0	0.0	0.0	0.0	7.322566881885043E-5
7	0.0	0.0	0.0	0.0	7.322566881885043E-5
8	0.0	0.0	0.0	0.0	7.322566881885043E-5
9	0.0	0.0	0.0	0.0	7.322566881885043E-5
10-11	0.0	0.0	0.0	0.0	7.322566881885043E-5
12-13	0.0	0.0	0.0	0.0	2.3188128459302636E-4
14-15	0.0	0.0	0.0	0.0	2.684941190024516E-4
16-17	0.0	0.0	0.0	0.0	3.1731123154835186E-4
18-19	0.0	0.0	0.0	0.0	3.1731123154835186E-4
20-21	0.0	0.0	0.0	2.4408556272950145E-5	4.027411785036774E-4
22-23	0.0	0.0	0.0	9.763422509180058E-5	4.1494545664015245E-4
24-25	0.0	0.0	0.0	1.5865561577417596E-4	4.1494545664015245E-4
26-27	0.0	0.0	0.0	5.12579681731953E-4	4.881711254590029E-4
28-29	0.0	0.0	0.0	0.002672736911888041	4.881711254590029E-4
30-31	0.0	0.0	0.0	0.010153959409547261	4.881711254590029E-4
32-33	0.0	0.0	0.0	0.02461602900127022	4.881711254590029E-4
34-35	0.0	0.0	0.0	0.049524960677815845	5.613967942778534E-4
36-37	0.0	0.0	0.0	0.09416821010104165	5.858053505508035E-4
38-39	0.0	0.0	0.0	0.16291490884380574	6.346224630967037E-4
40-41	0.0	0.0	0.0	0.2375318653702143	6.346224630967037E-4
42-43	0.0	0.0	0.0	0.316774243310347	6.590310193696539E-4
44-45	0.0	0.0	0.0	0.414701371077423	6.71235297506129E-4
46-47	0.0	0.0	0.0	0.5422848947161334	6.956438537790791E-4
48-49	0.0	0.0	0.0	0.6884677382348318	7.566652444614545E-4
50-51	0.0	0.0	0.0	0.8435719090712934	7.566652444614545E-4
52-53	0.0	0.0	0.0	0.9986150585170728	8.054823570073548E-4
54-55	0.0	0.0	0.0	1.1682789331703494	8.054823570073548E-4
56-57	0.0	0.0	0.0	1.3798889117786906	8.054823570073548E-4
58-59	0.0	0.0	0.0	1.6384731569343245	8.054823570073548E-4
60-61	0.0	0.0	0.0	1.9007308898090371	8.054823570073548E-4
62-63	0.0	0.0	0.0	2.1822713821393807	8.542994695532551E-4
64-65	2.4408556272950145E-5	0.0	0.0	2.4724891162247578	8.542994695532551E-4
66-67	2.4408556272950145E-5	0.0	0.0	2.803603386345463	8.542994695532551E-4
68-69	2.4408556272950145E-5	0.0	0.0	3.1820702556356917	8.542994695532551E-4
70-71	2.4408556272950145E-5	0.0	0.0	3.595002006383326	8.665037476897301E-4
72-73	2.4408556272950145E-5	0.0	0.0	4.025080767912707	9.397294165085805E-4
74-75	2.4408556272950145E-5	0.0	0.0	4.443882776443985	0.0010373636416003812
76-77	2.4408556272950145E-5	0.0	0.0	4.92176569543394	0.0010983850322827564
78-79	2.4408556272950145E-5	0.0	0.0	5.435150859522901	0.0010983850322827564
80-81	2.4408556272950145E-5	0.0	0.0	5.992056479446531	0.0010983850322827564
82-83	2.4408556272950145E-5	0.0	0.0	6.578166936950746	0.0011594064229651319
84-85	2.4408556272950145E-5	0.0	0.0	7.140308192194925	0.0011960192573745572
86-87	2.4408556272950145E-5	0.0	0.0	7.740294913940312	0.0012570406480569324
88	2.4408556272950145E-5	0.0	0.0	8.224658304620736	0.0013180620387393078
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4400	0.0	41.979805	94
GTACTAG	4995	0.0	33.79214	1
GCATCGT	4760	0.0	32.184746	8
TAGGCAT	5510	0.0	31.898869	5
CTGTGCG	6205	0.0	31.429327	9
TAGTACT	6235	0.0	31.129644	4
CAACGCA	6885	0.0	31.125488	5
CCGTGTT	515	0.0	31.02419	9
TGAACCG	505	0.0	30.709862	5
ACTGTGC	6710	0.0	29.554888	8
TATTCTC	2160	0.0	29.154549	5
GTATCAA	8425	0.0	29.075325	1
CGCCTAC	2280	0.0	29.061876	7
CTAACGC	2300	0.0	28.810572	3
TACTGTG	7640	0.0	28.725191	7
TCTAACG	2340	0.0	28.719757	2
GTACTGT	6925	0.0	28.70516	6
ATCAACG	7560	0.0	28.471102	3
TAACGCC	2345	0.0	28.45811	4
TACCCCG	215	1.8189894E-12	28.415817	5
>>END_MODULE