FastQCFastQC Report
Mon 16 Jan 2017
ERR1146659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096924
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79450.19392597958858893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71310.17405741478240747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.10524969464896101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC45750.042.96557694
GGTATCA78500.038.2973061
GTATCAA131850.037.573811
CTGTGCG62050.033.6337369
CAACGCA144100.032.875895
TAGTACT65200.032.3251764
ATCAACG146700.032.261043
ACTGTGC67900.031.9100888
GTACTAG49150.031.0140511
TACTGTG78050.030.5292427
GTACTGT70900.029.961436
TAGGCAT59650.029.7825785
TATCAAC168400.029.3069362
AACGCAG171100.028.8684226
CTAGTAC78450.027.7050863
TATTCTC25100.027.337585
TCTAACG21200.027.2964552
CTAGGCA68450.027.2852254
CTAACGC20750.026.979843
ACGCAGA177800.026.723937
Produced by FastQC (version 0.11.5)