##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4096924
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11422154767821	33.0	31.0	34.0	30.0	34.0
2	32.17799817619267	33.0	31.0	34.0	30.0	34.0
3	32.33774753937344	34.0	31.0	34.0	30.0	34.0
4	35.95327689749676	37.0	35.0	37.0	35.0	37.0
5	35.85935399338626	37.0	35.0	37.0	35.0	37.0
6	35.89825854714415	37.0	35.0	37.0	35.0	37.0
7	35.81946211352712	37.0	35.0	37.0	35.0	37.0
8	35.82293740376927	37.0	35.0	37.0	35.0	37.0
9	37.53360301533541	39.0	37.0	39.0	35.0	39.0
10-11	37.468428752888755	39.0	37.0	39.0	34.0	39.0
12-13	37.43759061676516	39.0	37.0	39.0	34.0	39.0
14-15	38.65265818941235	40.0	38.0	41.0	34.0	41.0
16-17	38.65291045184143	40.0	38.0	41.0	34.0	41.0
18-19	38.70571226119889	40.0	38.0	41.0	34.0	41.0
20-21	38.703146555806256	40.0	38.0	41.0	34.0	41.0
22-23	38.66412276625097	40.0	38.0	41.0	34.0	41.0
24-25	38.58907756160475	40.0	38.0	41.0	34.0	41.0
26-27	38.44604391001639	40.0	38.0	41.0	34.0	41.0
28-29	38.390585351351405	40.0	38.0	41.0	34.0	41.0
30-31	38.26260946017061	40.0	37.5	41.0	34.0	41.0
32-33	38.126534443890094	40.0	37.0	41.0	33.0	41.0
34-35	38.02901982072403	40.0	37.0	41.0	33.0	41.0
36-37	38.02142949197984	40.0	37.0	41.0	33.0	41.0
38-39	37.9284656000453	40.0	37.0	41.0	33.0	41.0
40-41	37.813780900011814	40.0	37.0	41.0	33.0	41.0
42-43	37.67963306129184	40.0	37.0	41.0	33.0	41.0
44-45	37.47644440072601	40.0	36.0	41.0	32.0	41.0
46-47	37.24066116432719	40.0	36.0	41.0	31.5	41.0
48-49	36.979545263714925	39.0	35.5	41.0	31.0	41.0
50-51	36.775331077657285	39.0	35.0	41.0	31.0	41.0
52-53	36.588189578327544	39.0	35.0	41.0	30.0	41.0
54-55	36.40216416023338	39.0	35.0	41.0	30.0	41.0
56-57	36.17631520623766	38.0	35.0	40.0	30.0	41.0
58-59	35.89772009439277	37.5	35.0	40.0	30.0	41.0
60-61	35.71460051492291	37.0	35.0	40.0	30.0	41.0
62-63	35.505987174768194	37.0	35.0	40.0	29.5	41.0
64-65	35.25896306594899	36.0	34.0	39.5	29.0	41.0
66-67	35.0103664358919	36.0	34.0	39.0	29.0	41.0
68-69	34.75855410058864	35.0	34.0	39.0	29.0	41.0
70-71	34.54588466859527	35.0	34.0	38.5	29.0	41.0
72-73	34.31360650087724	35.0	34.0	37.5	29.0	40.0
74-75	34.05798948186494	35.0	34.0	37.0	29.0	39.5
76-77	33.8143894053197	35.0	34.0	36.5	29.0	39.0
78-79	33.58963285625997	35.0	33.5	36.0	28.0	39.0
80-81	33.458988621707405	35.0	33.0	36.0	29.0	38.5
82-83	33.29620979056482	35.0	33.0	35.5	29.0	37.0
84-85	33.166100591565765	35.0	33.0	35.0	29.0	37.0
86-87	33.02765745715566	35.0	33.0	35.0	29.0	36.5
88-89	32.92240519960829	35.0	33.0	35.0	29.0	36.0
90-91	32.860404781733806	35.0	33.0	35.0	29.0	36.0
92-93	32.794345342993914	35.0	33.0	35.0	29.0	36.0
94-95	32.751928886159476	35.0	33.0	35.0	29.0	35.5
96-97	32.66777038090041	35.0	33.0	35.0	28.5	35.0
98-99	32.59327571124092	35.0	33.0	35.0	27.0	35.0
100	31.355040757407263	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39372715425729155
1101	2	0.45409304192899924
1101	3	0.37513235058614924
1101	4	0.24956184509471058
1101	5	0.2376039622095476
1101	6	0.24047156066408348
1101	7	0.26943831640176796
1101	8	0.30799135085107565
1101	9	0.3214586430970954
1101	10-11	0.3659105360751056
1101	12-13	0.3542591524188978
1101	14-15	0.4891933647904594
1101	16-17	0.5505334359183749
1101	18-19	0.4941975617628671
1101	20-21	0.5597781646734958
1101	22-23	0.5319466196346454
1101	24-25	0.5583792379494383
1101	26-27	0.5945451434069042
1101	28-29	0.6055545953913253
1101	30-31	0.6059005199284115
1101	32-33	0.5982768782407746
1101	34-35	0.5964170825713637
1101	36-37	0.47521068355429463
1101	38-39	0.5978688525914748
1101	40-41	0.5346418055365945
1101	42-43	0.47021900144626727
1101	44-45	0.5315015091312318
1101	46-47	0.5551744209155984
1101	48-49	0.5250028273287697
1101	50-51	0.5345353687286689
1101	52-53	0.5860638787610242
1101	54-55	0.6389511169821134
1101	56-57	0.6343951053563117
1101	58-59	0.573427178935205
1101	60-61	0.6210193563249362
1101	62-63	0.611019036228619
1101	64-65	0.6525950430552854
1101	66-67	0.6103918807002628
1101	68-69	0.5839232586919465
1101	70-71	0.5136215797496106
1101	72-73	0.5942271465021491
1101	74-75	0.5752515408619274
1101	76-77	0.5321675668849295
1101	78-79	0.4945560188370024
1101	80-81	0.5814723614951234
1101	82-83	0.47496685049653564
1101	84-85	0.5078240503958327
1101	86-87	0.5066203519678396
1101	88-89	0.48760401617807503
1101	90-91	0.45489365842801277
1101	92-93	0.4841314779760211
1101	94-95	0.4464571548353007
1101	96-97	0.4112503421591711
1101	98-99	0.46806337968090617
1101	100	0.7722361815975347
1102	1	0.3699538877750612
1102	2	0.43992662295037377
1102	3	0.3182810365763089
1102	4	0.26045084300167787
1102	5	0.2882425271061848
1102	6	0.27164066988752467
1102	7	0.293341482525733
1102	8	0.34063450013179164
1102	9	0.42153126905735405
1102	10-11	0.42313803623866164
1102	12-13	0.4319431629652186
1102	14-15	0.5837269300584609
1102	16-17	0.5444004771622062
1102	18-19	0.5198207501766774
1102	20-21	0.5394580267851339
1102	22-23	0.5497458026997819
1102	24-25	0.5289104420092983
1102	26-27	0.5806044720808003
1102	28-29	0.5951330397960888
1102	30-31	0.7289338204041798
1102	32-33	0.6675041759202145
1102	34-35	0.6405798626449979
1102	36-37	0.651332827943996
1102	38-39	0.6367375307201542
1102	40-41	0.6123718452575062
1102	42-43	0.6450180560225149
1102	44-45	0.6253209444799523
1102	46-47	0.6444871464194861
1102	48-49	0.6280575435418996
1102	50-51	0.5943633236239521
1102	52-53	0.6238868730722444
1102	54-55	0.5733249521625368
1102	56-57	0.575394859581543
1102	58-59	0.5222127376136143
1102	60-61	0.5144905024085062
1102	62-63	0.6267158561078006
1102	64-65	0.6145941559425268
1102	66-67	0.587228611428344
1102	68-69	0.5425875246863541
1102	70-71	0.511330563336081
1102	72-73	0.5139668437745684
1102	74-75	0.5442528834256137
1102	76-77	0.5064933286864388
1102	78-79	0.5133840950291173
1102	80-81	0.5001419081201988
1102	82-83	0.525809794333334
1102	84-85	0.5058910601882332
1102	86-87	0.468962581370441
1102	88-89	0.4743276006115238
1102	90-91	0.4887865108634344
1102	92-93	0.5000500345520038
1102	94-95	0.5420277872814196
1102	96-97	0.48755206671798845
1102	98-99	0.5124524744732071
1102	100	0.7729936713312213
1103	1	0.2813380306673281
1103	2	0.3523727082763628
1103	3	0.2665804530835487
1103	4	0.2031168658529623
1103	5	0.1679222556503106
1103	6	0.16004584352423734
1103	7	0.2232565398915014
1103	8	0.19200542915223195
1103	9	0.23411157007136296
1103	10-11	0.29288164992988186
1103	12-13	0.28218195340317465
1103	14-15	0.3967168556690055
1103	16-17	0.3973085267233145
1103	18-19	0.3752468065907095
1103	20-21	0.3323604237304423
1103	22-23	0.32839569360456977
1103	24-25	0.3611910050608813
1103	26-27	0.3908297608576561
1103	28-29	0.33396463704966095
1103	30-31	0.3454071637187326
1103	32-33	0.4191505817240042
1103	34-35	0.4269990459180306
1103	36-37	0.350560330933547
1103	38-39	0.31288824324975906
1103	40-41	0.3879727716674779
1103	42-43	0.3867262564286449
1103	44-45	0.37147505482352017
1103	46-47	0.35009292859476915
1103	48-49	0.39327963473553496
1103	50-51	0.39116058191522995
1103	52-53	0.39896786471264534
1103	54-55	0.31261437871661
1103	56-57	0.28914636792993775
1103	58-59	0.30368069641794193
1103	60-61	0.34705793163983856
1103	62-63	0.3446241547695408
1103	64-65	0.3556413233833524
1103	66-67	0.3908379505162003
1103	68-69	0.3866804665049983
1103	70-71	0.37883835299089696
1103	72-73	0.3304426577329238
1103	74-75	0.24656442862190886
1103	76-77	0.31858028828911245
1103	78-79	0.3317346796553906
1103	80-81	0.30064245783844257
1103	82-83	0.29085530237032486
1103	84-85	0.26093388173946863
1103	86-87	0.3714159035107656
1103	88-89	0.363679994645004
1103	90-91	0.2909100699317051
1103	92-93	0.2554671043385923
1103	94-95	0.1813788849056479
1103	96-97	0.26578660439062674
1103	98-99	0.28405459602318217
1103	100	0.5022739089908619
1104	1	0.26874634820190835
1104	2	0.2828120076755738
1104	3	0.231926858739385
1104	4	0.16648972505226567
1104	5	0.14516694914176043
1104	6	0.1977283271949659
1104	7	0.20176754959138066
1104	8	0.24283767598389971
1104	9	0.29475099520803383
1104	10-11	0.3138841581978511
1104	12-13	0.2798251497378743
1104	14-15	0.36392867567845855
1104	16-17	0.32218076219549374
1104	18-19	0.3217471557218232
1104	20-21	0.33862679483161884
1104	22-23	0.3645033770419204
1104	24-25	0.41146108400194237
1104	26-27	0.37196262022626314
1104	28-29	0.46082746879861247
1104	30-31	0.40578478350826686
1104	32-33	0.34363252313372783
1104	34-35	0.41680458934500564
1104	36-37	0.3280149131355685
1104	38-39	0.3427901624722338
1104	40-41	0.321249020950404
1104	42-43	0.29493019608788984
1104	44-45	0.315176164728463
1104	46-47	0.36687962079339087
1104	48-49	0.30893078774119687
1104	50-51	0.36681023760542075
1104	52-53	0.3977337566596404
1104	54-55	0.43175657831802283
1104	56-57	0.34037654090030145
1104	58-59	0.302016136618775
1104	60-61	0.34737228852762314
1104	62-63	0.37195290030355466
1104	64-65	0.3000557215795894
1104	66-67	0.2574068904525504
1104	68-69	0.284945749803839
1104	70-71	0.2625518197167054
1104	72-73	0.2248330552318265
1104	74-75	0.3040673085071788
1104	76-77	0.22790056129257863
1104	78-79	0.22749451236874307
1104	80-81	0.28050233450567674
1104	82-83	0.27102960206119775
1104	84-85	0.1593945038634672
1104	86-87	0.2585142555775164
1104	88-89	0.24618505370629862
1104	90-91	0.2264535220401669
1104	92-93	0.25806482765189287
1104	94-95	0.24811890504005163
1104	96-97	0.23322568038295088
1104	98-99	0.26000440262213687
1104	100	0.45423203918244326
1105	1	0.2350457081179158
1105	2	0.2506869434441441
1105	3	0.2074838860420556
1105	4	0.17444424101001488
1105	5	0.23874282337592945
1105	6	0.19116015165988642
1105	7	0.21532123169350115
1105	8	0.20529320540585871
1105	9	0.2278266971923486
1105	10-11	0.2206566898523903
1105	12-13	0.23783924121250521
1105	14-15	0.28780586997402224
1105	16-17	0.32321351789327935
1105	18-19	0.3133909208440855
1105	20-21	0.3210553565037415
1105	22-23	0.3027747243684402
1105	24-25	0.3200820821539381
1105	26-27	0.350611563932695
1105	28-29	0.33253654521934095
1105	30-31	0.4288692126505751
1105	32-33	0.3528720456929122
1105	34-35	0.31985649892429535
1105	36-37	0.3810622487062645
1105	38-39	0.399905265949414
1105	40-41	0.43126231959686834
1105	42-43	0.3299774847844503
1105	44-45	0.34405297722662453
1105	46-47	0.411429947866516
1105	48-49	0.3917510508764863
1105	50-51	0.44792154780561333
1105	52-53	0.3582444125875952
1105	54-55	0.3624160571128954
1105	56-57	0.2540869741509013
1105	58-59	0.35879120098880435
1105	60-61	0.36729117610858
1105	62-63	0.4127059182041819
1105	64-65	0.33392578899014325
1105	66-67	0.3038956143243112
1105	68-69	0.3385686922244986
1105	70-71	0.4342893734361013
1105	72-73	0.37494536415356094
1105	74-75	0.3611094420566374
1105	76-77	0.2673856967726991
1105	78-79	0.2656313611615886
1105	80-81	0.220160186062337
1105	82-83	0.2819073127283147
1105	84-85	0.2932628953975964
1105	86-87	0.2853900338205122
1105	88-89	0.25341478461094624
1105	90-91	0.2819714144781287
1105	92-93	0.2745713410149335
1105	94-95	0.2804927298159541
1105	96-97	0.27204641276363617
1105	98-99	0.2724483647165883
1105	100	0.4842894499206025
1106	1	0.15415396551301797
1106	2	0.22057645652278524
1106	3	0.1827656807985676
1106	4	0.1082081537243269
1106	5	0.09278766512903758
1106	6	0.13699992937655736
1106	7	0.18181790728894498
1106	8	0.07504303965850312
1106	9	0.08676543881222898
1106	10-11	0.19163751650853555
1106	12-13	0.15408233668928517
1106	14-15	0.17821437234055537
1106	16-17	0.18250606403942982
1106	18-19	0.15124150620991372
1106	20-21	0.08930888133286885
1106	22-23	0.20048273063265043
1106	24-25	0.1729750100888836
1106	26-27	0.20188751545832417
1106	28-29	0.17578933042968004
1106	30-31	0.1893087984058539
1106	32-33	0.25456669419939004
1106	34-35	0.2660680987657926
1106	36-37	0.18793119747275
1106	38-39	0.2352963399874355
1106	40-41	0.23070040331977992
1106	42-43	0.26957172749483505
1106	44-45	0.20214256373364492
1106	46-47	0.22459414372143982
1106	48-49	0.35472995403879537
1106	50-51	0.34366228935468257
1106	52-53	0.2697390342252959
1106	54-55	0.26960454544364865
1106	56-57	0.21167740285334702
1106	58-59	0.16848175903966478
1106	60-61	0.25257836401976164
1106	62-63	0.29922130979232264
1106	64-65	0.31981519633967537
1106	66-67	0.30593446443777594
1106	68-69	0.2930555350125914
1106	70-71	0.36485529577952036
1106	72-73	0.30341030840570227
1106	74-75	0.2717631292623963
1106	76-77	0.2996859402891445
1106	78-79	0.350904241693037
1106	80-81	0.2797327203551916
1106	82-83	0.24358817280067768
1106	84-85	0.26820543550554277
1106	86-87	0.24313326048868333
1106	88-89	0.27781485677834183
1106	90-91	0.25412823500006
1106	92-93	0.28368106794767556
1106	94-95	0.25371722036410915
1106	96-97	0.23343404430059422
1106	98-99	0.21639799206996457
1106	100	0.37440460250274654
1107	1	0.141929279473743
1107	2	0.20432933988453783
1107	3	0.18175816570335712
1107	4	0.13256692814142923
1107	5	0.08028370028169718
1107	6	0.06250086774375063
1107	7	0.15541492716332073
1107	8	0.13083637142108984
1107	9	0.13163489036526954
1107	10-11	0.181040181365816
1107	12-13	0.12420000807986042
1107	14-15	0.224138101394054
1107	16-17	0.15004383745793604
1107	18-19	0.12650601731125022
1107	20-21	0.19593587473334395
1107	22-23	0.1529090252602785
1107	24-25	0.13091010319860885
1107	26-27	0.1727682850010055
1107	28-29	0.2592113019941067
1107	30-31	0.2870717127713931
1107	32-33	0.33562650449134424
1107	34-35	0.21524837707528377
1107	36-37	0.1809360790935628
1107	38-39	0.22189904284965678
1107	40-41	0.2192842102354291
1107	42-43	0.2704140026286126
1107	44-45	0.26530036249838673
1107	46-47	0.2622891530283411
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2216	68-69	-0.8084271291768914
2216	70-71	-0.8669491838465007
2216	72-73	-0.8463922060311404
2216	74-75	-0.6746402748744629
2216	76-77	-0.737084387770814
2216	78-79	-0.7789011436793061
2216	80-81	-0.8358474454437541
2216	82-83	-0.7969398425032423
2216	84-85	-0.7107822649041182
2216	86-87	-0.7403307551864273
2216	88-89	-0.7235936174112751
2216	90-91	-0.7011978336742644
2216	92-93	-0.7845719919669136
2216	94-95	-0.749584452124985
2216	96-97	-0.7346665757054751
2216	98-99	-0.7771330990981866
2216	100	-0.3136446209913686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	11.0
13	56.0
14	220.0
15	655.0
16	1677.0
17	3279.0
18	5784.0
19	8963.0
20	12834.0
21	18365.0
22	24747.0
23	28734.0
24	29691.0
25	29274.0
26	31636.0
27	38461.0
28	49166.0
29	62298.0
30	78320.0
31	97815.0
32	123631.0
33	158502.0
34	227408.0
35	325898.0
36	531709.0
37	998355.0
38	876629.0
39	332036.0
40	767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.834666203229546	21.845364961615104	12.749980229069418	25.569988606085932
2	14.503570829652496	20.99069804794971	39.172673785787325	25.33305733661047
3	17.264220668970186	27.157545514634883	29.76318330532858	25.81505051106635
4	11.48616625510221	17.381570576871024	39.37589597109831	31.756367196928466
5	12.225682143883217	39.05461227464775	35.65706792672148	13.062637654747553
6	29.389373439530104	39.512507313415455	17.657105100584022	13.441014146470412
7	25.803993435074705	32.53457960167189	24.18238659052499	17.47904037272842
8	26.611574927921534	34.705330145250436	19.6505719901077	19.03252293672033
9	26.286379732697018	13.84533860037433	21.545310579351728	38.322971087576924
10-11	25.59571522439762	25.93492093092281	27.712010278931213	20.757353565748353
12-13	25.702172062498285	23.787644964348154	28.220507516330894	22.289675456822668
14-15	22.969025688895744	24.761958581794072	25.84673564818838	26.4222800811218
16-17	21.4524848398457	27.142534252527017	27.334934697348544	24.070046210278736
18-19	20.80028108900265	27.475266193677548	29.79465320680911	21.92979951051069
20-21	22.391530816778637	26.640999442508573	29.001746188115767	21.965723552597023
22-23	22.796566399571972	26.313155918928445	28.6965416004788	22.193736081020784
24-25	22.32109992765304	26.3279963211424	29.656188398906107	21.694715352298456
26-27	22.300279429152212	26.509534958422464	28.482576196190116	22.707609416235204
28-29	21.47079125705041	27.14304683220875	28.994167331393015	22.391994579347823
30-31	22.429827841570894	26.708196197928007	28.922223111778493	21.939752848722602
32-33	22.944213756467047	26.875053088609896	28.079493297898615	22.101239857024442
34-35	22.685934618264824	26.69378294544883	28.980254454317432	21.640027981968913
36-37	22.40179461469141	26.221404155898426	28.65407071256387	22.722730516846298
38-39	22.568749139598392	26.250181843744237	29.396249478877323	21.784819537780052
40-41	22.104229905167877	25.503316634626366	29.8769515861168	22.51550187408895
42-43	22.314180101949656	25.769589575007984	28.883682001423505	23.032548321618854
44-45	22.340089784433395	26.82541828942885	28.64373368898227	22.190758237155485
46-47	22.54015451592463	26.572814140560087	27.87092218454626	23.016109158969023
48-49	23.46565374412608	26.390225935360284	27.782782887844636	22.361337432669
50-51	22.710013659028093	26.261897950755248	27.65563871821884	23.37244967199782
52-53	22.001640743152667	26.993214909527246	27.48649962752543	23.518644719794658
54-55	22.16442140493697	26.942005758466596	27.271264978310555	23.622307858285872
56-57	23.451508985765905	26.24665480736279	27.00721321654978	23.29462299032152
58-59	22.069633470090423	26.7722109041089	28.08572090740772	23.07243471839296
60-61	22.327421743727733	27.444211803782547	27.105115935760587	23.123250516729136
62-63	22.767263927766297	26.5414003286368	26.702130671694178	23.989205071902724
64-65	21.81611774014165	26.903231341687007	27.181782023839613	24.09886889433173
66-67	22.49805555077554	26.403136894229164	27.1669182581982	23.93188929679709
68-69	21.990168721704382	26.313863767060358	27.631376613283525	24.06459089795173
70-71	22.030982705575937	25.990844049468155	27.63364774597174	24.344525498984172
72-73	22.62391468284019	25.922846814842877	28.12833064207182	23.324907860245116
74-75	22.705200633160885	26.39056800194293	27.53677471164208	23.367456653254102
76-77	22.397248078037187	26.36180094868863	27.112609147582507	24.128341825691678
78-79	22.083946394905055	26.41990673978819	27.260574030663005	24.235572834643747
80-81	21.715670097858784	26.89622751117668	27.812964067676138	23.5751383232884
82-83	22.51862616929189	25.717477307365233	28.921600693593536	22.842295829749343
84-85	22.938807261252588	26.290956336998196	27.32218122669593	23.448055175053284
86-87	21.327744913012786	26.988919003623206	27.883187484073417	23.80014859929059
88-89	22.42700865332137	25.782672561170283	27.629582584397465	24.160736201110883
90-91	21.963770868095185	25.731316958771995	27.834394780083787	24.47051739304903
92-93	22.434929229831944	26.004521929135127	27.48881844037136	24.07173040066157
94-95	21.14447326823734	26.038803746420484	28.185170142282356	24.63155284305982
96-97	21.501192113888372	25.934457168353624	27.737273134673725	24.827077583084282
98-99	20.854218921317553	26.594635389868102	27.59510549866192	24.956040190152418
100	21.73511656970333	25.784301537940014	26.664090855471343	25.81649103688531
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	233.0
1	152.5
2	111.0
3	151.5
4	101.5
5	81.5
6	188.5
7	411.0
8	632.5
9	670.0
10	563.5
11	453.5
12	447.0
13	525.5
14	676.5
15	977.0
16	1307.5
17	1782.5
18	2484.5
19	3268.0
20	4201.0
21	5216.0
22	6346.5
23	7551.0
24	8935.0
25	10413.5
26	12272.5
27	14181.5
28	17482.5
29	25507.5
30	37019.0
31	47775.5
32	62201.0
33	75619.5
34	83610.5
35	95052.5
36	106716.0
37	125291.5
38	133745.0
39	125537.5
40	116385.0
41	106673.0
42	105162.5
43	111450.5
44	122322.5
45	147903.0
46	171648.5
47	186480.0
48	197873.5
49	192597.0
50	181371.5
51	185105.5
52	187079.5
53	177238.5
54	168527.0
55	144213.0
56	123671.5
57	102311.0
58	78645.5
59	76813.0
60	62849.0
61	40245.0
62	28812.5
63	24106.0
64	17548.5
65	7440.5
66	3516.5
67	2360.5
68	1765.5
69	1334.0
70	1016.5
71	832.0
72	602.5
73	430.0
74	347.5
75	244.0
76	125.5
77	54.5
78	28.5
79	9.5
80	3.0
81	1.5
82	1.0
83	0.5
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.006272998962148188
3	0.0
4	0.08787080258262052
5	0.004173863122674475
6	2.440855627295015E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.806983971389267E-4
14-15	0.008982348708445654
16-17	0.0
18-19	2.440855627295015E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.2204278136475074E-5
60-61	0.0
62-63	0.0
64-65	8.54299469553255E-5
66-67	1.342470595012258E-4
68-69	0.0
70-71	6.224181849602287E-4
72-73	5.491925161413782E-4
74-75	4.637625691860527E-4
76-77	1.342470595012258E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.059093114736812294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4096924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.73436889887435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.33756244751282	47.4746082162153
2	16.003949249707258	20.72011109138826
3	5.4341676509432935	10.553322401234777
4	2.3525269055279487	6.091573781878943
5	1.1682872198353809	3.7814167934331935
6	0.6012613620413477	2.335336488901455
7	0.34595879943029106	1.56767971822925
8	0.21883687395983842	1.1333013542074122
9	0.14482380005410134	0.8437569568235114
>10	0.3804464951422158	3.547532920393283
>50	0.00659711534773333	0.29888791626533334
>100	0.00472771696822766	0.5807325271841152
>500	5.339772060259818E-4	0.25710694677828133
>1k	2.1359088237745118E-4	0.2935056580848343
>5k	1.0679544118872559E-4	0.5211272289820943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7945	0.19392597958858893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7131	0.17405741478240747	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4312	0.10524969464896101	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.322566881885043E-5
2	0.0	0.0	0.0	0.0	7.322566881885043E-5
3	0.0	0.0	0.0	0.0	7.322566881885043E-5
4	0.0	0.0	0.0	0.0	7.322566881885043E-5
5	0.0	0.0	0.0	0.0	7.322566881885043E-5
6	0.0	0.0	0.0	0.0	7.322566881885043E-5
7	0.0	0.0	0.0	0.0	7.322566881885043E-5
8	0.0	0.0	0.0	0.0	7.322566881885043E-5
9	0.0	0.0	0.0	0.0	7.322566881885043E-5
10-11	0.0	0.0	0.0	0.0	9.763422509180058E-5
12-13	0.0	0.0	0.0	0.0	1.830641720471261E-4
14-15	0.0	0.0	0.0	0.0	2.562898408659765E-4
16-17	0.0	0.0	0.0	0.0	3.2951550968482696E-4
18-19	0.0	0.0	0.0	0.0	3.661283440942522E-4
20-21	0.0	0.0	0.0	2.4408556272950145E-5	3.783326222307272E-4
22-23	0.0	0.0	0.0	9.763422509180058E-5	3.905369003672023E-4
24-25	0.0	0.0	0.0	1.830641720471261E-4	3.905369003672023E-4
26-27	0.0	0.0	0.0	5.369882380049032E-4	3.905369003672023E-4
28-29	0.0	0.0	0.0	0.002684941190024516	3.905369003672023E-4
30-31	0.0	0.0	0.0	0.010239389356502586	4.027411785036774E-4
32-33	0.0	0.0	0.0	0.024799093173317348	4.1494545664015245E-4
34-35	0.0	0.0	0.0	0.04990329330004657	4.881711254590029E-4
36-37	0.0	0.0	0.0	0.09469299406091009	4.881711254590029E-4
38-39	0.0	0.0	0.0	0.16402549815422499	5.00375403595478E-4
40-41	0.0	0.0	0.0	0.23877670174013477	5.12579681731953E-4
42-43	0.0	0.0	0.0	0.3182631652429969	5.12579681731953E-4
44-45	0.0	0.0	0.0	0.41655642135416715	5.12579681731953E-4
46-47	0.0	0.0	0.0	0.5447989760122472	5.369882380049032E-4
48-49	0.0	0.0	0.0	0.6918239147223624	5.858053505508035E-4
50-51	0.0	0.0	0.0	0.8476115251344667	6.102139068237536E-4
52-53	0.0	0.0	0.0	1.00347236121539	6.590310193696539E-4
54-55	0.0	0.0	0.0	1.1740393524507655	6.834395756426041E-4
56-57	0.0	0.0	0.0	1.3867233075351166	7.078481319155543E-4
58-59	0.0	0.0	0.0	1.6475653441459983	7.322566881885044E-4
60-61	0.0	1.2204278136475072E-5	0.0	1.9126422652702368	7.322566881885044E-4
62-63	0.0	2.4408556272950145E-5	0.0	2.1965625918371927	7.566652444614545E-4
64-65	0.0	2.4408556272950145E-5	0.0	2.48889166604018	7.810738007344046E-4
66-67	0.0	2.4408556272950145E-5	0.0	2.822764103019729	7.810738007344046E-4
68-69	0.0	2.4408556272950145E-5	0.0	3.2041111819501653	7.810738007344046E-4
70-71	0.0	2.4408556272950145E-5	0.0	3.6204113134634666	7.810738007344046E-4
72-73	0.0	2.4408556272950145E-5	0.0	4.05269904933555	8.176866351438299E-4
74-75	0.0	2.4408556272950145E-5	0.0	4.474051751997353	8.787080258262052E-4
76-77	0.0	2.4408556272950145E-5	0.0	4.955644771540795	8.787080258262052E-4
78-79	0.0	2.4408556272950145E-5	0.0	5.473191594474294	8.787080258262052E-4
80-81	0.0	2.4408556272950145E-5	0.0	6.035613548115611	9.031165820991554E-4
82-83	0.0	2.4408556272950145E-5	0.0	6.626532491205597	9.519336946450556E-4
84-85	0.0	2.4408556272950145E-5	0.0	7.194531799955284	9.519336946450556E-4
86-87	0.0	2.4408556272950145E-5	0.0	7.801987539920194	9.763422509180058E-4
88	0.0	2.4408556272950145E-5	0.0	8.293563658979274	0.001000750807190956
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4575	0.0	42.965576	94
GGTATCA	7850	0.0	38.297306	1
GTATCAA	13185	0.0	37.57381	1
CTGTGCG	6205	0.0	33.633736	9
CAACGCA	14410	0.0	32.87589	5
TAGTACT	6520	0.0	32.325176	4
ATCAACG	14670	0.0	32.26104	3
ACTGTGC	6790	0.0	31.910088	8
GTACTAG	4915	0.0	31.014051	1
TACTGTG	7805	0.0	30.529242	7
GTACTGT	7090	0.0	29.96143	6
TAGGCAT	5965	0.0	29.782578	5
TATCAAC	16840	0.0	29.306936	2
AACGCAG	17110	0.0	28.868422	6
CTAGTAC	7845	0.0	27.705086	3
TATTCTC	2510	0.0	27.33758	5
TCTAACG	2120	0.0	27.296455	2
CTAGGCA	6845	0.0	27.285225	4
CTAACGC	2075	0.0	26.97984	3
ACGCAGA	17780	0.0	26.72393	7
>>END_MODULE