FastQCFastQC Report
Mon 16 Jan 2017
ERR1146658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059506
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC55450.040.62060594
CTGTGCG69350.035.0983779
GTACTAG53900.034.197391
TAGTACT74400.033.7318954
ACTGTGC79200.032.2761548
CAACGCA75650.031.6185995
CTAGTAC85000.030.3549923
TAGGCAT66100.030.2149075
GCATCGT54700.030.0669088
GTATCAA95300.029.9496291
ATCAACG80800.029.7807253
TCTAACG26200.028.3424112
CGCCTAC25850.028.1762687
TACTGTG98000.028.0026477
CTAGGCA73750.027.7841364
GTACTGT93800.027.3530926
CTAACGC27000.027.32833
CCTAGTA96250.026.6607822
GTACATG192700.026.1094511
GGTATCA65200.025.9627441
Produced by FastQC (version 0.11.5)