##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146658_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5059506
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266628006765878	31.0	31.0	34.0	28.0	34.0
2	31.382423106129334	31.0	31.0	34.0	28.0	34.0
3	31.518690559908418	33.0	31.0	34.0	28.0	34.0
4	34.949441902035495	37.0	35.0	37.0	32.0	37.0
5	35.10501973908124	37.0	35.0	37.0	32.0	37.0
6	35.18515384703566	37.0	35.0	37.0	32.0	37.0
7	35.10407399457576	37.0	35.0	37.0	32.0	37.0
8	35.086790093736425	37.0	35.0	37.0	32.0	37.0
9	36.585419208910906	39.0	37.0	39.0	32.0	39.0
10-11	36.42449203538844	39.0	37.0	39.0	31.5	39.0
12-13	36.34124724824913	39.0	37.0	39.0	31.0	39.0
14-15	37.50876903792584	40.0	37.0	41.0	31.0	41.0
16-17	37.52873146113474	40.0	37.0	41.0	31.0	41.0
18-19	37.493368720187306	40.0	37.0	41.0	31.0	41.0
20-21	37.54887048261233	40.0	37.0	41.0	31.0	41.0
22-23	37.49043276161744	40.0	37.0	41.0	31.0	41.0
24-25	37.450191184672974	40.0	37.0	41.0	31.0	41.0
26-27	37.48538375090374	40.0	37.0	41.0	31.0	41.0
28-29	37.45996308730536	40.0	37.0	41.0	31.0	41.0
30-31	37.31589309312016	40.0	36.0	41.0	30.0	41.0
32-33	37.29350691549728	40.0	36.0	41.0	30.0	41.0
34-35	37.220204897474176	40.0	36.0	41.0	30.0	41.0
36-37	37.113174784257595	40.0	36.0	41.0	30.0	41.0
38-39	37.01107766252278	40.0	36.0	41.0	30.0	41.0
40-41	36.93048343059579	40.0	36.0	41.0	30.0	41.0
42-43	36.77107834243106	39.0	35.0	41.0	30.0	41.0
44-45	36.63168726353916	39.0	35.0	41.0	29.5	41.0
46-47	36.6355795407694	39.0	35.0	41.0	29.5	41.0
48-49	36.49789623730064	39.0	35.0	41.0	29.0	41.0
50-51	36.32606859246733	39.0	35.0	41.0	29.0	41.0
52-53	36.11886170309908	39.0	35.0	40.5	28.0	41.0
54-55	35.90505308225744	38.0	34.5	40.0	28.0	41.0
56-57	35.668886448597945	38.0	34.0	40.0	28.0	41.0
58-59	35.38039543781547	37.0	34.0	40.0	27.5	41.0
60-61	35.12746985575272	37.0	34.0	40.0	27.0	41.0
62-63	34.818119496251214	36.0	33.0	40.0	26.5	41.0
64-65	34.66511928239635	36.0	33.0	39.0	26.5	41.0
66-67	34.402971653754335	35.5	33.0	39.0	26.0	41.0
68-69	34.28180102958669	35.0	33.0	39.0	26.0	41.0
70-71	34.184772880988774	35.0	33.0	39.0	26.0	41.0
72-73	33.95167779225877	35.0	33.0	37.5	26.0	40.0
74-75	33.68920157422484	35.0	33.0	37.0	26.0	40.0
76-77	33.41764235480697	35.0	33.0	36.5	26.0	39.0
78-79	33.15748395199057	35.0	33.0	36.0	26.0	39.0
80-81	32.95081634452059	35.0	33.0	36.0	26.0	38.5
82-83	32.687311962867525	35.0	32.5	35.5	25.0	37.0
84-85	32.494661040030394	35.0	32.0	35.0	25.0	37.0
86-87	32.31313402929061	35.0	32.0	35.0	25.0	36.5
88-89	32.172692254935555	35.0	32.0	35.0	25.0	36.0
90-91	32.01133855755879	35.0	32.0	35.0	25.0	36.0
92-93	31.85372939571571	35.0	32.0	35.0	24.0	36.0
94-95	31.76681478389392	35.0	32.0	35.0	24.0	35.5
96-97	31.65455204519967	35.0	32.0	35.0	24.0	35.0
98-99	31.544396923335995	35.0	31.5	35.0	23.5	35.0
100	30.56392718972959	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37275748078938875
1101	2	0.44707217132243926
1101	3	0.5002813781558793
1101	4	0.5512661298837003
1101	5	0.4566850521749686
1101	6	0.43224220160780646
1101	7	0.4967698394861131
1101	8	0.48329919645938446
1101	9	0.635332299895822
1101	10-11	0.7028399591062211
1101	12-13	0.5560113771277884
1101	14-15	0.7849755259273223
1101	16-17	0.736085041691787
1101	18-19	0.7125421858947689
1101	20-21	0.7340459801184167
1101	22-23	0.6969525809679169
1101	24-25	0.7499331913475586
1101	26-27	0.70699551866592
1101	28-29	0.7092802611943299
1101	30-31	0.6985545850133619
1101	32-33	0.6825562821430537
1101	34-35	0.7479674939996812
1101	36-37	0.6945893591758718
1101	38-39	0.7199909013322454
1101	40-41	0.7326236506645927
1101	42-43	0.7253587387731955
1101	44-45	0.6944501959809486
1101	46-47	0.6972395493991002
1101	48-49	0.7064471905261911
1101	50-51	0.6355162096936979
1101	52-53	0.6377487192086235
1101	54-55	0.664451947340531
1101	56-57	0.6107360174484597
1101	58-59	0.597226362054144
1101	60-61	0.569799258443382
1101	62-63	0.5304783292545352
1101	64-65	0.6231967040633748
1101	66-67	0.7139794262671586
1101	68-69	0.7022006127743197
1101	70-71	0.6116364626302442
1101	72-73	0.5991653514536068
1101	74-75	0.5281537515187082
1101	76-77	0.5974347796264965
1101	78-79	0.600008718527512
1101	80-81	0.551977278400507
1101	82-83	0.5117005706867559
1101	84-85	0.5281756251163685
1101	86-87	0.5223245145262183
1101	88-89	0.5537836448070124
1101	90-91	0.5737886719673426
1101	92-93	0.5364774638529681
1101	94-95	0.5487986373406848
1101	96-97	0.5887328293428133
1101	98-99	0.5470833577809238
1101	100	0.7500839673620199
1102	1	0.3790037749778534
1102	2	0.4570596487004188
1102	3	0.4603104685564432
1102	4	0.5345289428113986
1102	5	0.36499116165182244
1102	6	0.45600409393390606
1102	7	0.42834838673525866
1102	8	0.464826862490888
1102	9	0.5592403012107354
1102	10-11	0.6646666100709169
1102	12-13	0.5677257304177772
1102	14-15	0.7881745197063736
1102	16-17	0.7042663895313765
1102	18-19	0.8035301684480416
1102	20-21	0.7259375412977676
1102	22-23	0.7582526576710293
1102	24-25	0.743858945362156
1102	26-27	0.723361860617274
1102	28-29	0.6763863810891095
1102	30-31	0.6980008983482833
1102	32-33	0.6792602069538276
1102	34-35	0.6758643387541312
1102	36-37	0.7325290089131826
1102	38-39	0.7315401282357286
1102	40-41	0.625599520273525
1102	42-43	0.6403484487303146
1102	44-45	0.656752692504206
1102	46-47	0.5822257655986363
1102	48-49	0.5863519134810673
1102	50-51	0.5552223750612981
1102	52-53	0.5640223393210277
1102	54-55	0.48282371552678427
1102	56-57	0.5004364599989088
1102	58-59	0.5032700347277768
1102	60-61	0.5090773880606108
1102	62-63	0.49737902371217757
1102	64-65	0.5474552543252713
1102	66-67	0.5868118711442705
1102	68-69	0.5395065408761184
1102	70-71	0.5100588633180223
1102	72-73	0.5227272619406733
1102	74-75	0.4775475880986093
1102	76-77	0.5183715031951834
1102	78-79	0.4936848647979275
1102	80-81	0.4754815266360879
1102	82-83	0.47749095884110915
1102	84-85	0.45327359228443953
1102	86-87	0.4817328118367712
1102	88-89	0.5009808018409672
1102	90-91	0.43547112624170126
1102	92-93	0.4951465346190389
1102	94-95	0.52850926786655
1102	96-97	0.48919388098496697
1102	98-99	0.46613846177593743
1102	100	0.48773351086235195
1103	1	0.24461789803998002
1103	2	0.28910032434235333
1103	3	0.34373543624517566
1103	4	0.4056149797817241
1103	5	0.2432996480680103
1103	6	0.31315052792400877
1103	7	0.32927346044604633
1103	8	0.4089160040155875
1103	9	0.4599395036416922
1103	10-11	0.5703560774331535
1103	12-13	0.5225860258021058
1103	14-15	0.6290588176897387
1103	16-17	0.6419375244916665
1103	18-19	0.6584706895353989
1103	20-21	0.5715431037721572
1103	22-23	0.6701148277704192
1103	24-25	0.6337857271334215
1103	26-27	0.5383802622543925
1103	28-29	0.5744641933144621
1103	30-31	0.5607898419888784
1103	32-33	0.5875834178922474
1103	34-35	0.5630632114775977
1103	36-37	0.5578674014319986
1103	38-39	0.6860334573079783
1103	40-41	0.6326758488855688
1103	42-43	0.5644227122264169
1103	44-45	0.5666868839795001
1103	46-47	0.5941769644195674
1103	48-49	0.5821141691879959
1103	50-51	0.5029968284134654
1103	52-53	0.5069905449411252
1103	54-55	0.4795800284179137
1103	56-57	0.5604236409757988
1103	58-59	0.5167339004647147
1103	60-61	0.542265587505014
1103	62-63	0.4915075378797269
1103	64-65	0.4592743201034466
1103	66-67	0.5102111040794526
1103	68-69	0.4921621818780295
1103	70-71	0.5037388060659396
1103	72-73	0.5038568591146486
1103	74-75	0.4622252107477536
1103	76-77	0.4090255192817551
1103	78-79	0.47279064536891724
1103	80-81	0.42059659406039884
1103	82-83	0.4631842736324643
1103	84-85	0.45753973608227483
1103	86-87	0.4716568911012331
1103	88-89	0.5292003299134009
1103	90-91	0.5614642871441688
1103	92-93	0.4947797256220028
1103	94-95	0.42194346299320173
1103	96-97	0.4150834275776809
1103	98-99	0.42713021023773123
1103	100	0.539266175831969
1104	1	0.23637592230167215
1104	2	0.34421063736459345
1104	3	0.26787007680340125
1104	4	0.4608138187446613
1104	5	0.3179845799828911
1104	6	0.2939256036438138
1104	7	0.33214729564650725
1104	8	0.36881066647455185
1104	9	0.4185181327055574
1104	10-11	0.5297982630862137
1104	12-13	0.43196010477959135
1104	14-15	0.5634622959018714
1104	16-17	0.49494593185953306
1104	18-19	0.5163729353410176
1104	20-21	0.4892899142907794
1104	22-23	0.5662943283959265
1104	24-25	0.5661778244467044
1104	26-27	0.5505783236391721
1104	28-29	0.4884382408596011
1104	30-31	0.46019789395963073
1104	32-33	0.5053347600376128
1104	34-35	0.5820324295334061
1104	36-37	0.5401025427913169
1104	38-39	0.47401657280968834
1104	40-41	0.5181942934392296
1104	42-43	0.5983462005345288
1104	44-45	0.5540485689333607
1104	46-47	0.4911359293391939
1104	48-49	0.5312219433234802
1104	50-51	0.49174886544092544
1104	52-53	0.5267431710310646
1104	54-55	0.5227170242462762
1104	56-57	0.5044402916911395
1104	58-59	0.5444695381963811
1104	60-61	0.5329764875517355
1104	62-63	0.5070017575816195
1104	64-65	0.45934959409784426
1104	66-67	0.4468646451954683
1104	68-69	0.42586957772802236
1104	70-71	0.414968304496675
1104	72-73	0.41702491397780506
1104	74-75	0.4436496563313952
1104	76-77	0.3597859899360145
1104	78-79	0.39526293826818915
1104	80-81	0.3929934512964266
1104	82-83	0.4465561919303198
1104	84-85	0.4367816767433439
1104	86-87	0.4146280097947681
1104	88-89	0.33834065533613256
1104	90-91	0.43021108316158774
1104	92-93	0.4062394796815596
1104	94-95	0.4412512792997667
1104	96-97	0.39480023373026185
1104	98-99	0.3893431304591566
1104	100	0.45423752419325325
1105	1	0.07040563990476656
1105	2	0.22974340062362586
1105	3	0.22516283706286444
1105	4	0.2790817396060419
1105	5	0.16200270550234563
1105	6	0.2118882754160012
1105	7	0.24420905786194425
1105	8	0.2995692527402767
1105	9	0.3453517948978657
1105	10-11	0.32269736977712427
1105	12-13	0.25420411423448286
1105	14-15	0.4006783230510962
1105	16-17	0.40771711358028284
1105	18-19	0.4130976444453367
1105	20-21	0.3564543219071794
1105	22-23	0.4275988361854388
1105	24-25	0.3627085380003763
1105	26-27	0.3976725694663372
1105	28-29	0.389962748274165
1105	30-31	0.4194996915918381
1105	32-33	0.3571913583859754
1105	34-35	0.47058443651052784
1105	36-37	0.3882042609818299
1105	38-39	0.390937988012503
1105	40-41	0.40089264969114424
1105	42-43	0.4651872659595
1105	44-45	0.39363183391714074
1105	46-47	0.4484195600516472
1105	48-49	0.26763063535525333
1105	50-51	0.4108039094814657
1105	52-53	0.42688853099494395
1105	54-55	0.41816188803148435
1105	56-57	0.3322087511469718
1105	58-59	0.4104137598863957
1105	60-61	0.4332050875029907
1105	62-63	0.3939450114419998
1105	64-65	0.36781172727556566
1105	66-67	0.33717435848667066
1105	68-69	0.3548765603180186
1105	70-71	0.3642091948686854
1105	72-73	0.34537095642170357
1105	74-75	0.22264919858469057
1105	76-77	0.22076866898840564
1105	78-79	0.3018047908456296
1105	80-81	0.28618126648549236
1105	82-83	0.3718026799351719
1105	84-85	0.27595416093311087
1105	86-87	0.2139110620747573
1105	88-89	0.2625732265915133
1105	90-91	0.20495801407152214
1105	92-93	0.2898901892670551
1105	94-95	0.25802869176547816
1105	96-97	0.17472644941488724
1105	98-99	0.15910586573783192
1105	100	0.29006122219291797
1106	1	0.12902765698733631
1106	2	0.2311227660774655
1106	3	0.18471961998942632
1106	4	0.3325527304407103
1106	5	0.22670731201219496
1106	6	0.25028200910143283
1106	7	0.22965469120313742
1106	8	0.24453764466017702
1106	9	0.3876120009338422
1106	10-11	0.4130546222587199
1106	12-13	0.27278885991120205
1106	14-15	0.4666611256933919
1106	16-17	0.33299141704829793
1106	18-19	0.39209060619347014
1106	20-21	0.4003389765140781
1106	22-23	0.3761102947486634
1106	24-25	0.37237625678993425
1106	26-27	0.43038090755609915
1106	28-29	0.36535756388946794
1106	30-31	0.43336875144171927
1106	32-33	0.39192855280103345
1106	34-35	0.3381930036700851
1106	36-37	0.3617015929402285
1106	38-39	0.4284239723911085
1106	40-41	0.4301309482327582
1106	42-43	0.41048661045967094
1106	44-45	0.40556805396113305
1106	46-47	0.36081854927801515
1106	48-49	0.21363737236638514
1106	50-51	0.2798416419441807
1106	52-53	0.20970106666818822
1106	54-55	0.2952637873067232
1106	56-57	0.2847609418964083
1106	58-59	0.3036887032531368
1106	60-61	0.2566462935567273
1106	62-63	0.2526369956481034
1106	64-65	0.2403957131914609
1106	66-67	0.2088947506450296
1106	68-69	0.23042941633383407
1106	70-71	0.21603889936103826
1106	72-73	0.20436140677342252
1106	74-75	0.26215766566890863
1106	76-77	0.1809684740380888
1106	78-79	0.17911219971871617
1106	80-81	0.17205396405502427
1106	82-83	0.23549706772521972
1106	84-85	0.1672708651438839
1106	86-87	0.14117629724546532
1106	88-89	0.20431656695168243
1106	90-91	0.20377599300623928
1106	92-93	0.2741724467587545
1106	94-95	0.2555420605120382
1106	96-97	0.214053458595437
1106	98-99	0.23979931555937029
1106	100	0.27197403107565066
1107	1	0.06255587330744916
1107	2	0.14219155243031523
1107	3	0.19255469191926977
1107	4	0.2698879225038979
1107	5	0.11345785511503692
1107	6	0.20277777940647468
1107	7	0.12563673126761188
1107	8	0.16740953245562906
1107	9	0.23164712992702619
1107	10-11	0.3009421774449734
1107	12-13	0.2331894045157128
1107	14-15	0.3627021687511842
1107	16-17	0.16762442218399798
1107	18-19	0.17128851587321492
1107	20-21	0.1899509457999784
1107	22-23	0.25605265576939473
1107	24-25	0.3859400000449469
1107	26-27	0.2363381450897606
1107	28-29	0.27043219673192453
1107	30-31	0.32908061661206034
1107	32-33	0.26573954550293877
1107	34-35	0.280724713868409
1107	36-37	0.3064025234731318
1107	38-39	0.4142099448205059
1107	40-41	0.29352456514753555
1107	42-43	0.33546884073527394
1107	44-45	0.32598402819252215
1107	46-47	0.27883999057785047
1107	48-49	0.24318373132483373
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2216	92-93	-1.0589595002728984
2216	94-95	-1.0630813939086572
2216	96-97	-1.1347583410565925
2216	98-99	-1.1572768629820835
2216	100	-0.730201383159045
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	15.0
3	22.0
4	41.0
5	25.0
6	42.0
7	196.0
8	486.0
9	840.0
10	1251.0
11	2244.0
12	4380.0
13	6722.0
14	9355.0
15	12472.0
16	15415.0
17	18138.0
18	20969.0
19	23806.0
20	27758.0
21	31203.0
22	35957.0
23	41626.0
24	47080.0
25	54801.0
26	61113.0
27	67823.0
28	78152.0
29	93608.0
30	109720.0
31	127294.0
32	158124.0
33	202589.0
34	286283.0
35	406624.0
36	649421.0
37	1101986.0
38	994692.0
39	366420.0
40	812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15783179635694	20.034576777592257	11.516970090217228	24.290621335833578
2	15.840624613940157	19.727798779433204	39.78717663627604	24.6443999703506
3	18.847933344890027	26.20502747811525	28.704020879532933	26.24301829746179
4	12.08770225731198	16.098217324836835	40.23110377373036	31.582976644120826
5	12.978553127197051	38.678609992332994	35.38855569867921	12.954281181790744
6	30.348128117851758	38.389638399857056	17.991300503046524	13.270932979244662
7	27.201914828855255	31.721922892956954	23.564787546497058	17.511374731690736
8	26.33683925909499	35.04623244418249	19.920390304528134	18.696537992194383
9	26.15636997909862	14.874310081579612	21.185992617811138	37.783327321510626
10-11	25.660487706150096	25.81548384112916	27.841818162772032	20.682210289948713
12-13	25.644276447830205	23.608084485136704	27.8651212814089	22.88251778562419
14-15	22.951576891411406	25.05441501035034	25.041963175972782	26.952044922265472
16-17	22.192825247271088	27.66613746182289	26.304603588018722	23.836433702887305
18-19	21.44307262858579	28.471705379663554	27.89021735338551	22.195004638365145
20-21	23.164909003188676	27.54974193901606	27.011809356052215	22.27353970174305
22-23	23.490893278913717	27.263249957999385	26.583413216851635	22.662443546235263
24-25	22.99346460389902	27.209161235656413	27.489616704271224	22.307757456173334
26-27	23.041910131514253	27.370984837441657	26.441950156517528	23.145154874526554
28-29	22.089295729409876	27.961200261893293	26.968170082397556	22.981333926299275
30-31	22.910333997299006	27.654715449283714	26.975177632642012	22.459772920775272
32-33	23.274035673867782	27.717332812038126	26.103360150189946	22.90527136390415
34-35	23.164264308306194	27.505092567519757	27.124953600612255	22.205689523561798
36-37	22.8241442630987	27.120667946211668	26.75852771941199	23.296660071277643
38-39	23.071977008154477	27.33895874533151	27.256257677867087	22.332806568646927
40-41	22.696099951118686	26.513171171765933	27.807992302329644	22.982736574785743
42-43	22.675883649755303	26.677828783732828	27.231144283376764	23.415143283135105
44-45	22.816111881874352	27.599724660263426	27.14240330787245	22.44176014998978
46-47	22.70184325402081	27.36021993636917	26.69543509864677	23.24250171096325
48-49	23.410710759054872	27.079265437017845	26.867064327933942	22.64295947599334
50-51	22.930945439647253	26.769469054758037	27.0491625848992	23.250422920695513
52-53	22.480507743178364	27.409508055837357	26.815198963510234	23.294785237474045
54-55	22.441162141301003	27.295594985234377	26.83471115142576	23.428531722038855
56-57	23.482757962898372	26.73222203256096	26.543014333745855	23.242005670794814
58-59	22.45244165018657	27.187648508647012	27.38326479037399	22.976645050792428
60-61	22.459076588541986	27.804030195579386	26.67866912119003	23.058224094688594
62-63	22.82814326130734	27.14420765229853	26.276841728129675	23.75080735826446
64-65	22.133660464400528	27.318827566419422	26.762395727713596	23.785116241466454
66-67	22.658463031058268	26.9167338431498	26.774961483066978	23.649841642724954
68-69	22.193130179889035	26.965170589200333	27.040527906420543	23.801171324490088
70-71	22.172485211662103	26.65231483988904	27.12149346498377	24.05370648346509
72-73	22.739248951859096	26.566686627490217	27.561225848235342	23.13283857241534
74-75	22.86745951258448	26.832117385658304	27.08080542824265	23.219617673514563
76-77	22.571730920504685	26.78957536417822	26.80575458367567	23.832939131641425
78-79	22.508460591842628	26.762818089442646	26.710168257082994	24.018553061631735
80-81	22.066404690273533	27.091083578112585	27.383089081198186	23.459422650415696
82-83	22.8579199447816	26.117972012477114	28.402471308114375	22.621636734626914
84-85	22.98153349579722	26.622916658636374	27.19216291140658	23.203386934159827
86-87	21.71037260443586	27.215193876780525	27.649495813235635	23.424937705547972
88-89	22.88558969297277	26.06461628687172	27.345839284230046	23.703954735925468
90-91	22.48689205800397	26.140827563835767	27.453554016759306	23.918726361400953
92-93	22.828891216060487	26.30248144225603	27.35384973613748	23.514777605545994
94-95	21.78594936283511	26.33364827549401	27.94511111594286	23.93529124572802
96-97	22.0524817843099	26.25828678868673	27.57250078399393	24.116730643009436
98-99	21.447294582602712	26.826502904792655	27.42163404890204	24.304568463702594
100	22.276271801101423	26.10948061805045	26.538735164549887	25.075512416298235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	563.0
1	403.0
2	284.0
3	323.5
4	311.0
5	264.0
6	232.0
7	241.5
8	255.0
9	252.0
10	234.0
11	252.5
12	275.5
13	274.0
14	301.0
15	463.5
16	569.0
17	670.5
18	933.5
19	1225.0
20	1643.0
21	2262.5
22	3289.5
23	4800.5
24	6485.5
25	8652.0
26	12069.0
27	16016.5
28	21232.5
29	30857.5
30	44183.5
31	57139.5
32	74285.5
33	91611.5
34	104701.0
35	119166.0
36	131350.0
37	151449.5
38	160694.0
39	152284.0
40	143111.5
41	134243.5
42	134101.0
43	143303.0
44	158678.5
45	184589.0
46	207947.5
47	224706.5
48	236600.5
49	235192.5
50	225126.5
51	227949.5
52	229522.0
53	217939.5
54	206089.5
55	178663.5
56	155659.5
57	129640.0
58	103010.5
59	98634.5
60	82519.0
61	56590.0
62	42143.5
63	34979.5
64	25235.5
65	12672.0
66	7276.5
67	4968.5
68	3694.5
69	2852.0
70	2226.5
71	1782.0
72	1462.0
73	1256.0
74	948.5
75	699.0
76	461.5
77	241.5
78	144.5
79	92.5
80	56.5
81	40.0
82	33.5
83	29.5
84	25.5
85	18.5
86	14.5
87	13.0
88	7.0
89	10.5
90	10.5
91	7.0
92	10.5
93	7.0
94	4.5
95	6.0
96	5.0
97	4.0
98	4.5
99	7.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05797008640764533
2	0.0075303794481121275
3	0.007688497651747029
4	0.00948709221809402
5	0.0033007175008785443
6	0.002628715135430218
7	9.091796709006769E-4
8	0.001423063832714103
9	6.127080390852387E-4
10-11	4.941193863590635E-4
12-13	2.470596931795317E-4
14-15	2.0753014227080667E-4
16-17	5.632961004493325E-4
18-19	0.0014329462204412842
20-21	8.498853445375893E-4
22-23	0.0011068274254443023
24-25	0.0011364745886258461
26-27	0.0033303646640600883
28-29	0.0038244840504191514
30-31	0.005109194454952716
32-33	0.006176492329488295
34-35	0.0033995413781503572
36-37	0.008222146589014817
38-39	0.006344492920850376
40-41	0.006186374717215475
42-43	0.01439863891850311
44-45	0.02031818916708469
46-47	0.018816066232553136
48-49	0.021681958673435708
50-51	0.01845041788664743
52-53	0.02528903019385687
54-55	0.01906312592573267
56-57	0.0154066424666756
58-59	0.01735347284893031
60-61	0.016177468709395737
62-63	0.011236274845805106
64-65	0.011374628273985643
66-67	0.013677224614418879
68-69	0.005455078025404061
70-71	0.008933678505371868
72-73	0.008488971057648712
74-75	0.011018862315807116
76-77	0.01082121456126349
78-79	0.012066395414888331
80-81	0.010396271888994696
82-83	0.006384022471759101
84-85	0.01058403725581114
86-87	0.009249914912641669
88-89	0.007797203916746023
90-91	0.012758162555791019
92-93	0.008538382996284618
94-95	0.007431555570840315
96-97	0.009378385953095026
98-99	0.00895344328082623
100	0.2631877499502916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5059506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.45309395699071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1144665406646	50.66703169096684
2	15.019908211867156	20.26278559680903
3	5.104294692226653	10.329014083768003
4	2.1296789596811467	5.7461373986239455
5	1.0612817293606889	3.579336810270207
6	0.5468436541937882	2.213177783167064
7	0.3285813964617845	1.5514682265639177
8	0.206469688504621	1.1141615438378243
9	0.1297555891474211	0.7877174351585111
>10	0.3537307956980767	3.3853685761947125
>50	0.004024985872857125	0.1772137119668691
>100	8.403176854958375E-4	0.1050724905007572
>500	8.229242383847317E-5	0.04343562502008482
>1k	4.1146211893339255E-5	0.038079027152310874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.976477545436254E-5	0.0
3	0.0	0.0	0.0	1.976477545436254E-5	0.0
4	0.0	0.0	0.0	1.976477545436254E-5	0.0
5	0.0	0.0	0.0	1.976477545436254E-5	0.0
6	0.0	0.0	0.0	1.976477545436254E-5	1.976477545436254E-5
7	0.0	0.0	0.0	1.976477545436254E-5	1.976477545436254E-5
8	0.0	0.0	0.0	7.905910181745016E-5	1.976477545436254E-5
9	0.0	0.0	0.0	7.905910181745016E-5	1.976477545436254E-5
10-11	1.976477545436254E-5	0.0	0.0	1.2847104045335652E-4	1.976477545436254E-5
12-13	1.976477545436254E-5	0.0	0.0	1.8776536681644414E-4	1.976477545436254E-5
14-15	1.976477545436254E-5	0.0	0.0	1.976477545436254E-4	1.976477545436254E-5
16-17	1.976477545436254E-5	0.0	0.0	2.1741252999798796E-4	1.976477545436254E-5
18-19	1.976477545436254E-5	0.0	0.0	2.1741252999798796E-4	3.952955090872508E-5
20-21	1.976477545436254E-5	0.0	0.0	2.2729491772516922E-4	7.905910181745016E-5
22-23	1.976477545436254E-5	0.0	0.0	2.470596931795318E-4	7.905910181745016E-5
24-25	1.976477545436254E-5	0.0	0.0	3.360011827241632E-4	8.894148954463144E-5
26-27	1.976477545436254E-5	0.0	0.0	6.324728145396013E-4	9.88238772718127E-5
28-29	1.976477545436254E-5	0.0	0.0	0.003241423174515457	9.88238772718127E-5
30-31	1.976477545436254E-5	0.0	9.88238772718127E-6	0.011641452742619535	9.88238772718127E-5
32-33	1.976477545436254E-5	0.0	1.976477545436254E-5	0.027977039655650175	9.88238772718127E-5
34-35	1.976477545436254E-5	0.0	1.976477545436254E-5	0.05515360590539867	9.88238772718127E-5
36-37	1.976477545436254E-5	0.0	1.976477545436254E-5	0.10219377148678152	9.88238772718127E-5
38-39	1.976477545436254E-5	0.0	1.976477545436254E-5	0.17157801571932121	1.1858865272617524E-4
40-41	1.976477545436254E-5	0.0	1.976477545436254E-5	0.24998487994677743	1.1858865272617524E-4
42-43	1.976477545436254E-5	0.0	1.976477545436254E-5	0.33164304973647624	1.1858865272617524E-4
44-45	1.976477545436254E-5	0.0	1.976477545436254E-5	0.43100057594555674	1.1858865272617524E-4
46-47	1.976477545436254E-5	0.0	1.976477545436254E-5	0.5542141861280527	1.1858865272617524E-4
48-49	1.976477545436254E-5	0.0	1.976477545436254E-5	0.6946824452822074	1.1858865272617524E-4
50-51	1.976477545436254E-5	0.0	1.976477545436254E-5	0.8471676879126144	1.383534281805378E-4
52-53	1.976477545436254E-5	0.0	1.976477545436254E-5	0.9978148064257657	1.383534281805378E-4
54-55	1.976477545436254E-5	0.0	1.976477545436254E-5	1.1653706903401242	1.383534281805378E-4
56-57	1.976477545436254E-5	0.0	1.976477545436254E-5	1.3681671688896109	1.383534281805378E-4
58-59	1.976477545436254E-5	0.0	1.976477545436254E-5	1.6135863857064305	1.383534281805378E-4
60-61	1.976477545436254E-5	0.0	1.976477545436254E-5	1.8599444293573324	1.383534281805378E-4
62-63	1.976477545436254E-5	0.0	1.976477545436254E-5	2.125138304016242	1.383534281805378E-4
64-65	1.976477545436254E-5	0.0	1.976477545436254E-5	2.400501155646421	1.4823581590771905E-4
66-67	1.976477545436254E-5	0.0	1.976477545436254E-5	2.7179926261575735	1.5811820363490032E-4
68-69	1.976477545436254E-5	0.0	1.976477545436254E-5	3.0733929359901935	1.5811820363490032E-4
70-71	1.976477545436254E-5	0.0	1.976477545436254E-5	3.463431014806584	1.5811820363490032E-4
72-73	1.976477545436254E-5	0.0	1.976477545436254E-5	3.8733722225055174	1.976477545436254E-4
74-75	1.976477545436254E-5	0.0	1.976477545436254E-5	4.272175979235918	2.371773054523505E-4
76-77	1.976477545436254E-5	0.0	1.976477545436254E-5	4.721992621414027	2.371773054523505E-4
78-79	1.976477545436254E-5	0.0	1.976477545436254E-5	5.213740234718568	2.371773054523505E-4
80-81	1.976477545436254E-5	0.0	1.976477545436254E-5	5.747754820332261	2.371773054523505E-4
82-83	1.976477545436254E-5	1.976477545436254E-5	1.976477545436254E-5	6.305358665450738	2.371773054523505E-4
84-85	1.976477545436254E-5	1.976477545436254E-5	1.976477545436254E-5	6.840391137000331	2.371773054523505E-4
86-87	1.976477545436254E-5	1.976477545436254E-5	1.976477545436254E-5	7.410239260512785	2.470596931795318E-4
88	3.952955090872508E-5	1.976477545436254E-5	1.976477545436254E-5	7.873871480733494	2.5694208090671305E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5545	0.0	40.620605	94
CTGTGCG	6935	0.0	35.098377	9
GTACTAG	5390	0.0	34.19739	1
TAGTACT	7440	0.0	33.731895	4
ACTGTGC	7920	0.0	32.276154	8
CAACGCA	7565	0.0	31.618599	5
CTAGTAC	8500	0.0	30.354992	3
TAGGCAT	6610	0.0	30.214907	5
GCATCGT	5470	0.0	30.066908	8
GTATCAA	9530	0.0	29.949629	1
ATCAACG	8080	0.0	29.780725	3
TCTAACG	2620	0.0	28.342411	2
CGCCTAC	2585	0.0	28.176268	7
TACTGTG	9800	0.0	28.002647	7
CTAGGCA	7375	0.0	27.784136	4
GTACTGT	9380	0.0	27.353092	6
CTAACGC	2700	0.0	27.3283	3
CCTAGTA	9625	0.0	26.660782	2
GTACATG	19270	0.0	26.109451	1
GGTATCA	6520	0.0	25.962744	1
>>END_MODULE