FastQCFastQC Report
Mon 16 Jan 2017
ERR1146658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059506
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107210.2118981576462208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88200.17432531950747762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.11265922008986647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86500.041.8221131
GTATCAA149250.038.580051
GGGCACC50750.037.9020594
GTGGTAT22950.035.4616661
GTACTAG55750.032.2341
CTGTGCG67100.032.0138059
CAACGCA171650.031.5157685
ATCAACG174300.031.1169913
TAGTACT74150.029.3721354
TATCAAC196800.029.2106932
ACTGTGC80050.029.0034168
CTAACGC23200.028.183343
TAGGCAT69300.028.0100065
AACGCAG202850.027.7093816
TCTAACG23950.027.4990862
GGCGTCG35350.027.1221628
TAACGCC25000.026.718624
CGCCTAC24800.026.5308597
CTAGTAC85800.026.261163
CTAGGCA76500.026.0102544
Produced by FastQC (version 0.11.5)