##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5059506
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.155789517790865	33.0	31.0	34.0	30.0	34.0
2	32.204513049297695	33.0	31.0	34.0	30.0	34.0
3	32.363736894471515	34.0	31.0	34.0	30.0	34.0
4	35.96372037111924	37.0	35.0	37.0	35.0	37.0
5	35.87497514579486	37.0	35.0	37.0	35.0	37.0
6	35.91733343136662	37.0	35.0	37.0	35.0	37.0
7	35.83194960140377	37.0	35.0	37.0	35.0	37.0
8	35.843238055256776	37.0	35.0	37.0	35.0	37.0
9	37.55536291487746	39.0	37.0	39.0	35.0	39.0
10-11	37.49490157734767	39.0	37.0	39.0	34.5	39.0
12-13	37.465739540579655	39.0	37.0	39.0	34.0	39.0
14-15	38.69411569034605	40.0	38.0	41.0	34.0	41.0
16-17	38.69055249662714	40.0	38.0	41.0	34.0	41.0
18-19	38.7495412595617	40.0	38.0	41.0	34.0	41.0
20-21	38.74780512168579	40.0	38.0	41.0	34.0	41.0
22-23	38.70828634257969	40.0	38.0	41.0	34.0	41.0
24-25	38.642135319139854	40.0	38.0	41.0	34.0	41.0
26-27	38.50466972467272	40.0	38.0	41.0	34.0	41.0
28-29	38.44649398577647	40.0	38.0	41.0	34.0	41.0
30-31	38.32132623224481	40.0	38.0	41.0	34.0	41.0
32-33	38.187093364450995	40.0	37.0	41.0	33.5	41.0
34-35	38.089824283240304	40.0	37.0	41.0	33.0	41.0
36-37	38.082582667161574	40.0	37.0	41.0	33.0	41.0
38-39	37.989302414109204	40.0	37.0	41.0	33.0	41.0
40-41	37.884363216487934	40.0	37.0	41.0	33.0	41.0
42-43	37.74909170974399	40.0	37.0	41.0	33.0	41.0
44-45	37.551113389330894	40.0	36.5	41.0	32.0	41.0
46-47	37.31823235311906	40.0	36.0	41.0	31.5	41.0
48-49	37.07485898820952	39.5	36.0	41.0	31.0	41.0
50-51	36.871729473193625	39.0	35.0	41.0	31.0	41.0
52-53	36.69064608283892	39.0	35.0	41.0	31.0	41.0
54-55	36.512063628346326	39.0	35.0	41.0	30.0	41.0
56-57	36.29626301461052	38.5	35.0	41.0	30.0	41.0
58-59	36.03280112722467	38.0	35.0	40.0	30.0	41.0
60-61	35.85940830982313	37.0	35.0	40.0	30.0	41.0
62-63	35.65073932119064	37.0	35.0	40.0	30.0	41.0
64-65	35.40965343256832	36.5	34.5	40.0	29.0	41.0
66-67	35.15874445054517	36.0	34.0	39.0	29.0	41.0
68-69	34.89943603189718	35.5	34.0	39.0	29.0	41.0
70-71	34.66760638291564	35.0	34.0	39.0	29.0	41.0
72-73	34.414991700770784	35.0	34.0	38.0	29.0	40.0
74-75	34.15056796058746	35.0	34.0	37.0	29.0	39.5
76-77	33.897812750889116	35.0	34.0	37.0	29.0	39.0
78-79	33.66407224341665	35.0	34.0	36.0	29.0	39.0
80-81	33.521904114749546	35.0	34.0	36.0	29.0	38.5
82-83	33.35339369100461	35.0	33.0	36.0	29.0	37.0
84-85	33.220813949029804	35.0	33.0	35.0	29.0	37.0
86-87	33.07160007320873	35.0	33.0	35.0	29.0	36.5
88-89	32.96145503138054	35.0	33.0	35.0	29.0	36.0
90-91	32.893556208847265	35.0	33.0	35.0	29.0	36.0
92-93	32.82324440370266	35.0	33.0	35.0	29.0	36.0
94-95	32.775754194184174	35.0	33.0	35.0	29.0	35.5
96-97	32.68398594645406	35.0	33.0	35.0	28.5	35.0
98-99	32.60921095854022	35.0	33.0	35.0	27.5	35.0
100	31.379620460969903	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3624462634054524
1101	2	0.4025340599846743
1101	3	0.3611060424815733
1101	4	0.3039236690043339
1101	5	0.2289515385378067
1101	6	0.2892308997036608
1101	7	0.3378160618391348
1101	8	0.29360822611202053
1101	9	0.3844267911685293
1101	10-11	0.4118395038606195
1101	12-13	0.382294075149737
1101	14-15	0.48086826954779127
1101	16-17	0.5267359184913403
1101	18-19	0.5393635520326541
1101	20-21	0.5066568431593836
1101	22-23	0.5180747947723958
1101	24-25	0.5025629054448189
1101	26-27	0.5599574208814033
1101	28-29	0.560612808507436
1101	30-31	0.610430122342855
1101	32-33	0.6392485874241487
1101	34-35	0.6928134586548609
1101	36-37	0.5573382593132905
1101	38-39	0.5987968868126075
1101	40-41	0.5848724727910266
1101	42-43	0.5620854543700631
1101	44-45	0.5598360953482953
1101	46-47	0.5732523977260442
1101	48-49	0.5066443825298776
1101	50-51	0.49552558110537603
1101	52-53	0.53486250798219
1101	54-55	0.4890727489783231
1101	56-57	0.4879183111902847
1101	58-59	0.5228818664441945
1101	60-61	0.5684086343548458
1101	62-63	0.5189088411813714
1101	64-65	0.5593618713989628
1101	66-67	0.5175467983595965
1101	68-69	0.5263045810076861
1101	70-71	0.49607154436298373
1101	72-73	0.531181666869962
1101	74-75	0.5345217533321787
1101	76-77	0.5200312827150242
1101	78-79	0.5128368189446277
1101	80-81	0.5045345149304765
1101	82-83	0.48924745912682255
1101	84-85	0.48310697109391754
1101	86-87	0.4442796932981281
1101	88-89	0.46124227228132497
1101	90-91	0.41426480292990675
1101	92-93	0.41631912400686844
1101	94-95	0.4431727590565586
1101	96-97	0.4755586962082248
1101	98-99	0.5028722203905431
1101	100	0.8760784441252163
1102	1	0.34761785523789257
1102	2	0.38739991869937285
1102	3	0.29564008808156217
1102	4	0.2066922079167881
1102	5	0.17793458290289976
1102	6	0.19635197072219768
1102	7	0.2748874811423718
1102	8	0.2665477948992745
1102	9	0.31812353160298557
1102	10-11	0.31410439262937473
1102	12-13	0.3312578205517198
1102	14-15	0.44684847972230557
1102	16-17	0.42104857858767986
1102	18-19	0.4074543765899534
1102	20-21	0.42724729992184507
1102	22-23	0.4802450728675183
1102	24-25	0.44501228291541395
1102	26-27	0.4607090715000126
1102	28-29	0.4736854099161505
1102	30-31	0.46610813539200535
1102	32-33	0.5496598676856834
1102	34-35	0.5518703740540687
1102	36-37	0.558637273106946
1102	38-39	0.5288873021614933
1102	40-41	0.5127299570462398
1102	42-43	0.5359363565643278
1102	44-45	0.5570405613512648
1102	46-47	0.5925426205780369
1102	48-49	0.6535577098893341
1102	50-51	0.5864180660539446
1102	52-53	0.615277767701663
1102	54-55	0.6172659061408652
1102	56-57	0.5289558257935525
1102	58-59	0.5364109088613205
1102	60-61	0.5487163008884792
1102	62-63	0.5603727514164376
1102	64-65	0.5380295953437795
1102	66-67	0.5240609903125986
1102	68-69	0.49533461394849354
1102	70-71	0.48198946053485514
1102	72-73	0.4987729591777992
1102	74-75	0.4982821721325834
1102	76-77	0.44939097714146214
1102	78-79	0.46772528736249086
1102	80-81	0.4704670936215578
1102	82-83	0.39901635961621196
1102	84-85	0.4158450399352489
1102	86-87	0.4716836343223747
1102	88-89	0.47874191504520525
1102	90-91	0.4126707907001048
1102	92-93	0.4170328336852336
1102	94-95	0.4598516386232845
1102	96-97	0.42545195945054104
1102	98-99	0.44934428781044744
1102	100	0.6947545376021189
1103	1	0.2469496587735307
1103	2	0.31520231683303734
1103	3	0.23539674025984425
1103	4	0.19875185542147733
1103	5	0.20019254158259514
1103	6	0.23596598452488848
1103	7	0.23107704722393407
1103	8	0.19161948543336393
1103	9	0.22556045443997164
1103	10-11	0.28710517388898893
1103	12-13	0.2853863443237685
1103	14-15	0.3726892503725949
1103	16-17	0.3749203807723944
1103	18-19	0.34066339461008965
1103	20-21	0.35315343083177453
1103	22-23	0.3156266314744087
1103	24-25	0.3686631288389606
1103	26-27	0.4112750057359946
1103	28-29	0.4672729010984398
1103	30-31	0.3758357225401099
1103	32-33	0.3919320817576093
1103	34-35	0.3837961269187318
1103	36-37	0.42919995605502237
1103	38-39	0.41944145631602936
1103	40-41	0.4498629006920041
1103	42-43	0.4490645898334549
1103	44-45	0.5143786100290768
1103	46-47	0.5481160416024196
1103	48-49	0.5123822730995684
1103	50-51	0.4682980096066629
1103	52-53	0.43667277720820863
1103	54-55	0.37106139231529767
1103	56-57	0.3400091101035443
1103	58-59	0.3454159182193308
1103	60-61	0.4467464880551191
1103	62-63	0.42653851208922333
1103	64-65	0.4071171020340145
1103	66-67	0.4327254920924659
1103	68-69	0.45370394551705573
1103	70-71	0.4085169941362281
1103	72-73	0.3711508175858853
1103	74-75	0.38263682203483285
1103	76-77	0.37022835947415444
1103	78-79	0.3938431643175164
1103	80-81	0.373594602744717
1103	82-83	0.4130079759168126
1103	84-85	0.42429335441802607
1103	86-87	0.40370512249715773
1103	88-89	0.32619424018385956
1103	90-91	0.3631092417947599
1103	92-93	0.324289415527133
1103	94-95	0.29551075064081544
1103	96-97	0.3345797209070227
1103	98-99	0.32244811373377047
1103	100	0.6354431903208315
1104	1	0.22755091598885713
1104	2	0.23310986537680378
1104	3	0.22889245966073446
1104	4	0.195912607555897
1104	5	0.1920571693013784
1104	6	0.21495555031960123
1104	7	0.18415173391951356
1104	8	0.22745720636119415
1104	9	0.25032586804571366
1104	10-11	0.2853638044578375
1104	12-13	0.3310757727881395
1104	14-15	0.385015985461429
1104	16-17	0.3899922528623563
1104	18-19	0.31637358135426297
1104	20-21	0.3700071875454327
1104	22-23	0.3689146671567727
1104	24-25	0.4105643378083812
1104	26-27	0.3979654220460205
1104	28-29	0.3987502221927528
1104	30-31	0.3603895135863624
1104	32-33	0.36673204840483464
1104	34-35	0.4354212270554356
1104	36-37	0.34473971738086107
1104	38-39	0.3343263853306624
1104	40-41	0.40525116655161497
1104	42-43	0.40286561056155534
1104	44-45	0.4475739565530148
1104	46-47	0.42626669795833294
1104	48-49	0.4157869259093516
1104	50-51	0.4185303375361329
1104	52-53	0.35655511070300605
1104	54-55	0.38401422675229213
1104	56-57	0.34962164572948495
1104	58-59	0.3640892822873809
1104	60-61	0.35184109470403513
1104	62-63	0.32637866079321043
1104	64-65	0.30362964048180885
1104	66-67	0.25793680307199907
1104	68-69	0.277000631952383
1104	70-71	0.2853025285566062
1104	72-73	0.20912120534028844
1104	74-75	0.2588579117259613
1104	76-77	0.2213608203444153
1104	78-79	0.23313619919252915
1104	80-81	0.2783323866548031
1104	82-83	0.19858004553670838
1104	84-85	0.2257362586646039
1104	86-87	0.2196532740245658
1104	88-89	0.27455816616186723
1104	90-91	0.25383819976164546
1104	92-93	0.27622788867560644
1104	94-95	0.2504230732166022
1104	96-97	0.2655928809470325
1104	98-99	0.23800455634505369
1104	100	0.45791237721864064
1105	1	0.17713833857811068
1105	2	0.13312531237505
1105	3	0.06702412667110025
1105	4	0.1447604880168285
1105	5	0.17033802580325386
1105	6	0.1352512851514689
1105	7	0.20022168948840147
1105	8	0.19557438717270514
1105	9	0.16583625561499815
1105	10-11	0.15544312508675517
1105	12-13	0.1733674863230732
1105	14-15	0.25732647949978826
1105	16-17	0.25990055315356386
1105	18-19	0.2557891263071781
1105	20-21	0.2738742781355441
1105	22-23	0.21285560650611757
1105	24-25	0.24249928817489064
1105	26-27	0.2741494803532589
1105	28-29	0.2851127978068817
1105	30-31	0.305884018673126
1105	32-33	0.3203292949725167
1105	34-35	0.2700252273397652
1105	36-37	0.3190227598966189
1105	38-39	0.24070908109939637
1105	40-41	0.29730799197671587
1105	42-43	0.29921664291315153
1105	44-45	0.2898078658972949
1105	46-47	0.33969579291656515
1105	48-49	0.364363019267806
1105	50-51	0.3060863939311531
1105	52-53	0.28705802083155874
1105	54-55	0.29700207654575905
1105	56-57	0.19110185482442432
1105	58-59	0.2663802322601967
1105	60-61	0.2855765967881112
1105	62-63	0.32489161869613525
1105	64-65	0.3050290486918783
1105	66-67	0.25265958315673487
1105	68-69	0.23226106717107342
1105	70-71	0.3094513520850981
1105	72-73	0.259315942971746
1105	74-75	0.30247107592735745
1105	76-77	0.21571129066742856
1105	78-79	0.24187459148613755
1105	80-81	0.15113695029565122
1105	82-83	0.20452777453503757
1105	84-85	0.24679187449795137
1105	86-87	0.25798096754362376
1105	88-89	0.24638667870381425
1105	90-91	0.2389527418899604
1105	92-93	0.26053546672688555
1105	94-95	0.2494363010026106
1105	96-97	0.24737186740522787
1105	98-99	0.24340983402388616
1105	100	0.42410161251606837
1106	1	0.13815750256215154
1106	2	0.14795707418305426
1106	3	0.12823088435686003
1106	4	0.13134059395209619
1106	5	0.09353527442895881
1106	6	0.13688337572409637
1106	7	0.15561897210674402
1106	8	0.11729083149199937
1106	9	0.07340453261948454
1106	10-11	0.1527063847524559
1106	12-13	0.14168135316324992
1106	14-15	0.15907053649579694
1106	16-17	0.21067956168469948
1106	18-19	0.20629600347452026
1106	20-21	0.1836229355531671
1106	22-23	0.20174729112039103
1106	24-25	0.2002554601218378
1106	26-27	0.23855105454219228
1106	28-29	0.17444437316273564
1106	30-31	0.21936366938608387
1106	32-33	0.24544630321446448
1106	34-35	0.23008422612972623
1106	36-37	0.1719529760542926
1106	38-39	0.21180641229677377
1106	40-41	0.21679804532486457
1106	42-43	0.25776629880111557
1106	44-45	0.24485042962714942
1106	46-47	0.2945660469481126
1106	48-49	0.3321616693288192
1106	50-51	0.3448043102077989
1106	52-53	0.3613233581070219
1106	54-55	0.3553128009694717
1106	56-57	0.3004492437331834
1106	58-59	0.20122944066297777
1106	60-61	0.21440901308124438
1106	62-63	0.3217890135662884
1106	64-65	0.2555439325207658
1106	66-67	0.32889830089860794
1106	68-69	0.3313853920747434
1106	70-71	0.3154214360266181
1106	72-73	0.2978839840571794
1106	74-75	0.2624387195018656
1106	76-77	0.25074847328654215
1106	78-79	0.2888685102285109
1106	80-81	0.21917887602934627
1106	82-83	0.2222336265960294
1106	84-85	0.2649559771303558
1106	86-87	0.177383391548517
1106	88-89	0.23907320379368002
1106	90-91	0.19517810356909848
1106	92-93	0.2455872674488475
1106	94-95	0.25602457848908244
1106	96-97	0.17315647267205492
1106	98-99	0.19361461430207072
1106	100	0.3549772971252665
1107	1	0.12347583090661374
1107	2	0.14060591439568526
1107	3	0.13461634722063565
1107	4	0.16892969285187576
1107	5	0.039794372027294855
1107	6	0.040366241705335426
1107	7	0.08217069451862358
1107	8	0.08146318780750761
1107	9	0.11870193773916782
1107	10-11	0.1319995877077318
1107	12-13	0.07272412313792387
1107	14-15	0.18089713902460858
1107	16-17	0.1266788208623879
1107	18-19	0.08630856742050241
1107	20-21	0.13986298701028232
1107	22-23	0.1032784281825272
1107	24-25	0.0936821011934228
1107	26-27	0.1258017152756068
1107	28-29	0.14581672146935176
1107	30-31	0.0898392097358709
1107	32-33	0.18882582948862847
1107	34-35	0.1426424157201751
1107	36-37	0.10429149681232985
1107	38-39	0.15539998866654514
1107	40-41	0.21262503828705093
1107	42-43	0.21244399103949974
1107	44-45	0.1980429107812327
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2216	66-67	-0.8165402488001448
2216	68-69	-0.7180256899969706
2216	70-71	-0.7896634367708018
2216	72-73	-0.7415700910538661
2216	74-75	-0.6749937780238326
2216	76-77	-0.6700406423981207
2216	78-79	-0.6546123422752146
2216	80-81	-0.7288332662624128
2216	82-83	-0.6992091106081375
2216	84-85	-0.6964100675413931
2216	86-87	-0.7353402290176518
2216	88-89	-0.6901093388904656
2216	90-91	-0.6641623807929022
2216	92-93	-0.7438821897591268
2216	94-95	-0.7706374798205147
2216	96-97	-0.7569752598569544
2216	98-99	-0.7901265562802138
2216	100	-0.25029001267298767
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	16.0
13	65.0
14	268.0
15	739.0
16	1855.0
17	3648.0
18	6697.0
19	10581.0
20	15053.0
21	21710.0
22	30025.0
23	35559.0
24	35751.0
25	35505.0
26	38623.0
27	47006.0
28	58700.0
29	74839.0
30	94263.0
31	119201.0
32	150099.0
33	192030.0
34	272079.0
35	395601.0
36	641850.0
37	1202850.0
38	1146841.0
39	426922.0
40	1126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53713939661303	21.219364103926353	12.744544625502966	25.498951873957655
2	14.589471174933353	20.67853289138568	39.28771420964235	25.44428172403862
3	17.545902702753985	26.46942211354231	29.469635968412728	26.51503921529098
4	11.592816697761286	16.924625732022605	38.81504672303724	32.66751084717887
5	12.38189322275675	38.70860370181719	35.502230644996466	13.40727243042959
6	30.280729043651505	38.81745348606237	17.37284576257954	13.528971707706583
7	26.434003635928093	32.308193724841914	23.562873529550117	17.694929109679876
8	26.630485268719912	34.80901099830695	19.48455046797059	19.07595326500255
9	26.11343874283379	14.244058609674543	21.276365716336734	38.36613693115494
10-11	25.5941884444845	25.958581727148854	27.554794875230904	20.89243495313574
12-13	25.764211947718874	23.585910683145524	28.194662983030117	22.455214386105485
14-15	22.942460020569126	24.760888382161117	25.820042106811197	26.476609490458564
16-17	21.641608884345626	27.32516771400212	27.125237127893513	23.90798627375874
18-19	20.99116622981559	27.424711253839185	29.684497760600863	21.899624755744362
20-21	22.46932803321115	26.579215441191295	29.077888236519534	21.873568289078023
22-23	22.7251929338556	26.261822794557414	28.739544927904028	22.27343934368296
24-25	22.28925116404645	26.20553271406339	29.605953624721465	21.899262497168696
26-27	22.35578928061356	26.426710433785434	28.565881728374272	22.651618557226733
28-29	21.55156056737555	27.003920936154636	29.008019755288363	22.436498741181452
30-31	22.412158420209405	26.658007718540112	28.98456884921176	21.945265012038725
32-33	22.81245441748661	26.739092709841632	28.25264956697354	22.195803305698224
34-35	22.630144128695566	26.62508948502087	29.022606159573684	21.72216022670988
36-37	22.347250897617275	26.19393079087168	28.638873044127234	22.81994526738381
38-39	22.503214740727653	26.360281023483324	29.2646455997878	21.871858636001225
40-41	22.148397491770936	25.689148308154984	29.62879182275898	22.533662377315096
42-43	22.350274908261795	25.75066617175669	28.865634312915134	23.033424607066383
44-45	22.49616859827817	26.64584249924795	28.637746451926333	22.220242450547545
46-47	22.449800435062237	26.55902572306466	27.94024752614188	23.050926315731218
48-49	23.33609249598676	26.482970867116272	27.772770701329343	22.408165935567624
50-51	22.692788584498167	26.33426069659765	27.738844464261923	23.23410625464225
52-53	22.139987579815106	26.96269161455684	27.52776654479706	23.369554260831
54-55	22.21253418812034	26.866031980197274	27.427124308183448	23.494309523498934
56-57	23.289556332179465	26.32538631241864	27.14222495239654	23.24283240300535
58-59	22.150852701993575	26.818819232316205	27.99457654454339	23.035751521146832
60-61	22.294627182970036	27.399038562262795	27.206114589052767	23.1002196657144
62-63	22.676077466851506	26.693327372277054	26.81015695998779	23.820438200883643
64-65	21.971004066111245	26.870754585060485	27.253677609606875	23.904563739221395
66-67	22.437150156848517	26.510033255265665	27.314747239326735	23.738069348559083
68-69	22.130026132986107	26.35968808021969	27.625572536132974	23.88471325066123
70-71	22.094789338632953	26.151884110677326	27.598445097892917	24.154881452796804
72-73	22.65486151958459	26.06224187111423	27.94054457684256	23.34235203245862
74-75	22.739826557428923	26.414272046885408	27.455544136957037	23.39035725872863
76-77	22.420554371204414	26.462437577799218	27.021198545784582	24.095809505211783
78-79	22.25281151543595	26.542490475104824	27.07573439256749	24.12896361689173
80-81	21.865939085752643	26.986666287182974	27.638350463464217	23.509044163600162
82-83	22.667707084446583	26.005206832445698	28.578135889155977	22.748950193951742
84-85	22.9402831027377	26.512608147910093	27.221155583173534	23.325953166178675
86-87	21.554989755916882	27.084907103578885	27.68423438968152	23.67586875082271
88-89	22.535836502615076	26.088871126944014	27.43210503159795	23.94318733884296
90-91	22.165474257763503	26.129962095113633	27.549339797205498	24.155223849917363
92-93	22.561896359051655	26.345072028771188	27.206490119786398	23.88654149239076
94-95	21.458191768129144	26.407548483982428	27.803188690753604	24.331071057134825
96-97	21.80599252179956	26.30748930824472	27.427074896244815	24.459443273710914
98-99	21.130017436484906	26.969293049558594	27.31489003076585	24.58579948319065
100	21.899686313304205	26.304155256027162	26.420643159579132	25.375515271089498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	246.5
2	183.5
3	270.0
4	197.5
5	143.5
6	309.0
7	682.0
8	1085.5
9	1149.0
10	883.0
11	672.0
12	717.0
13	823.0
14	978.5
15	1380.5
16	1793.5
17	2278.0
18	3085.0
19	4065.0
20	5210.0
21	6697.5
22	8499.5
23	10485.0
24	12504.5
25	14650.5
26	17924.0
27	21573.5
28	26252.5
29	35332.5
30	47687.0
31	59141.0
32	75373.0
33	91679.5
34	102880.5
35	116866.5
36	128658.0
37	147795.0
38	157604.5
39	149722.5
40	141129.5
41	132530.5
42	132000.5
43	140799.5
44	154800.5
45	181072.5
46	205631.0
47	223244.5
48	236962.5
49	234270.5
50	222590.0
51	224603.0
52	225774.5
53	214041.5
54	205242.0
55	178244.0
56	152786.5
57	127201.0
58	100960.0
59	98287.0
60	81719.0
61	55134.5
62	40556.5
63	32939.5
64	23400.5
65	11364.0
66	6361.5
67	4340.0
68	3279.0
69	2525.5
70	2022.5
71	1638.0
72	1256.5
73	1101.0
74	830.5
75	580.5
76	364.0
77	156.5
78	65.5
79	23.5
80	8.0
81	2.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007609438549929578
3	0.0
4	0.08904031342190324
5	0.0040913085190530454
6	1.976477545436254E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.5694208090671305E-4
14-15	0.009230150137187306
16-17	0.0
18-19	2.9647163181543808E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.976477545436254E-5
60-61	0.0
62-63	0.0
64-65	6.91767140902689E-5
66-67	1.2847104045335652E-4
68-69	0.0
70-71	4.644722231775197E-4
72-73	3.5576595817852575E-4
74-75	4.2494267226879465E-4
76-77	1.5811820363490032E-4
78-79	2.9647163181543808E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.06299033937305341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5059506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42591657620709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30847569105985	47.22965739201127
2	15.858971282522589	20.434575216645282
3	5.528349362910587	10.685069245770153
4	2.389249232456169	6.157182869199519
5	1.1464983079369617	3.693210217095464
6	0.6245612650262378	2.4142759174386454
7	0.34749929041314953	1.567157220611411
8	0.22730182730995807	1.171530285111263
9	0.15165457846485486	0.8793436698518932
>10	0.40667057897091446	3.7869554381553288
>50	0.006171377992534372	0.2651625900429645
>100	0.003512908561147264	0.45870929544058303
>500	6.939497033718415E-4	0.30337406167467196
>1k	2.602311144620695E-4	0.24849455072369422
>5k	8.674370482068815E-5	0.405728199314966
>10k+	4.3371852410344074E-5	0.29957383091293044
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10721	0.2118981576462208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8820	0.17432531950747762	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5700	0.11265922008986647	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.976477545436254E-5	0.0
8	0.0	0.0	0.0	3.952955090872508E-5	0.0
9	0.0	0.0	0.0	7.905910181745016E-5	0.0
10-11	0.0	0.0	0.0	8.894148954463144E-5	0.0
12-13	0.0	0.0	0.0	1.383534281805378E-4	0.0
14-15	0.0	0.0	0.0	1.383534281805378E-4	9.88238772718127E-6
16-17	0.0	0.0	0.0	1.383534281805378E-4	1.976477545436254E-5
18-19	0.0	0.0	0.0	1.383534281805378E-4	3.952955090872508E-5
20-21	0.0	0.0	0.0	1.4823581590771905E-4	6.91767140902689E-5
22-23	0.0	0.0	0.0	1.680005913620816E-4	7.905910181745016E-5
24-25	0.0	0.0	0.0	2.470596931795318E-4	7.905910181745016E-5
26-27	0.0	0.0	0.0	5.534137127221511E-4	9.88238772718127E-5
28-29	0.0	0.0	0.0	0.0031722464604251877	9.88238772718127E-5
30-31	0.0	0.0	0.0	0.011690864681255443	9.88238772718127E-5
32-33	0.0	0.0	0.0	0.028253746512011253	9.88238772718127E-5
34-35	0.0	0.0	0.0	0.05548960708812284	1.1858865272617524E-4
36-37	0.0	0.0	0.0	0.10279659713813957	1.1858865272617524E-4
38-39	0.0	0.0	0.0	0.1725860192674937	1.1858865272617524E-4
40-41	0.0	0.0	0.0	0.25113123692313044	1.1858865272617524E-4
42-43	0.0	0.0	0.0	0.33301670163055447	1.1858865272617524E-4
44-45	0.0	0.0	0.0	0.4326212875328145	1.2847104045335652E-4
46-47	0.0	0.0	0.0	0.5562894875507609	1.383534281805378E-4
48-49	0.0	0.0	0.0	0.6976471616003618	1.5811820363490032E-4
50-51	0.0	0.0	0.0	0.8505276997398561	1.5811820363490032E-4
52-53	0.0	0.0	0.0	1.002360704780269	1.7788297908926287E-4
54-55	0.0	0.0	0.0	1.1713001229764328	1.7788297908926287E-4
56-57	0.0	0.0	0.0	1.376073079071356	1.7788297908926287E-4
58-59	0.0	0.0	0.0	1.6239727752076982	1.7788297908926287E-4
60-61	0.0	0.0	0.0	1.8728014157903954	1.7788297908926287E-4
62-63	0.0	0.0	0.0	2.140100239035194	1.7788297908926287E-4
64-65	0.0	0.0	0.0	2.4177854517812607	1.7788297908926287E-4
66-67	0.0	0.0	0.0	2.73788587265239	1.7788297908926287E-4
68-69	0.0	0.0	0.0	3.0966363119245237	1.7788297908926287E-4
70-71	0.0	0.0	0.0	3.4900047554049745	1.7788297908926287E-4
72-73	0.0	0.0	0.0	3.903137974339787	1.7788297908926287E-4
74-75	0.0	0.0	0.0	4.306102216303331	1.7788297908926287E-4
76-77	0.0	0.0	0.0	4.760494403999125	1.7788297908926287E-4
78-79	0.0	0.0	0.0	5.258378980082245	1.7788297908926287E-4
80-81	0.0	0.0	0.0	5.798728176229063	1.7788297908926287E-4
82-83	0.0	0.0	0.0	6.363081692165204	1.7788297908926287E-4
84-85	0.0	0.0	0.0	6.904982423185189	1.7788297908926287E-4
86-87	0.0	0.0	0.0	7.481698806168033	1.8776536681644414E-4
88	0.0	0.0	0.0	7.950341396966423	1.976477545436254E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8650	0.0	41.822113	1
GTATCAA	14925	0.0	38.58005	1
GGGCACC	5075	0.0	37.90205	94
GTGGTAT	2295	0.0	35.461666	1
GTACTAG	5575	0.0	32.234	1
CTGTGCG	6710	0.0	32.013805	9
CAACGCA	17165	0.0	31.515768	5
ATCAACG	17430	0.0	31.116991	3
TAGTACT	7415	0.0	29.372135	4
TATCAAC	19680	0.0	29.210693	2
ACTGTGC	8005	0.0	29.003416	8
CTAACGC	2320	0.0	28.18334	3
TAGGCAT	6930	0.0	28.010006	5
AACGCAG	20285	0.0	27.709381	6
TCTAACG	2395	0.0	27.499086	2
GGCGTCG	3535	0.0	27.122162	8
TAACGCC	2500	0.0	26.71862	4
CGCCTAC	2480	0.0	26.530859	7
CTAGTAC	8580	0.0	26.26116	3
CTAGGCA	7650	0.0	26.010254	4
>>END_MODULE